Luminol chemiluminescence method for detection of DNA dot-hybridization by using peroxidase labels.

Abstract A novel chemiluminescence method has been developed for the determination of the organophosphorous pesticide phosphamidon in environmental water samples, based on the reaction of phosphamidon with luminolH2O2 in an alkaline medium using sodium dodecyl benzene sulfonate as the enhancer. Under optimum conditions, the increased chemiluminescence intensity was proportional to the concentration of phosphamidon in the range of 0.011.0 g/mL and the detection limit was 0.0038 g/mL (3). The relative standard deviation was <2 for 0.5 g/mL phosphamidon (n = 11). The proposed method was applied to the determination of phosphamidon residue in an environmental water sample with satisfactory results. Further study was focused on the mechanism of phosphamidon, and a possible mechanism was proposed.

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Porphyrin-Induced Photogeneration of Hydrogen Peroxide Determined Using the Luminol Chemiluminescence Method in Aqueous Solution: A Structure-Activity Relationship Study Related to the Aggregation of Porphyrin

Analytical Sciences ◽

10.2116/analsci.22.255 ◽

2006 ◽

Vol 22 (2) ◽

pp. 255-258 ◽

Cited By ~ 19

Author(s):

Keiko KOMAGOE ◽

Takashi KATSU

Keyword(s):

Aqueous Solution ◽

Hydrogen Peroxide ◽

Structure Activity Relationship ◽

Activity Relationship ◽

Chemiluminescence Method ◽

Luminol Chemiluminescence ◽

Structure Activity ◽

Relationship Study

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Are specimens with "at risk" biochemical profiles more likely to be infectious for hepatitis B virus?

Clinical Chemistry ◽

10.1093/clinchem/32.10.1951 ◽

1986 ◽

Vol 32 (10) ◽

pp. 1951-1953

Author(s):

L Burnett ◽

J B Whitfield ◽

B N Nightingale

Keyword(s):

At Risk ◽

Hepatitis B Virus ◽

Hepatitis B ◽

Hbv Dna ◽

Biochemical Tests ◽

Dot Hybridization ◽

B Virus ◽

Biochemical Profiles ◽

Dna Dot Hybridization ◽

Predictive Feature

Abstract The potential infectivity of 640 plasma specimens with various biochemical profiles was directly assessed by hepatitis B virus (HBV) DNA dot-hybridization. We found that specimens with "at risk" biochemical profiles typical of various forms of liver disease were not significantly more likely to carry HBV than the general patient population. Specimens containing HBV cannot be distinguished from non-infectious specimens by any simple biochemical tests, including aminotransferases and bilirubin. The only predictive feature of HBV-positive samples was that they were more likely to be labeled as "biohazardous" by the medical staff, but even this was not always the case.

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False-positive results with hepatitis B virus DNA dot-hybridization in hepatitis B surface antigen-negative specimens.

Journal of Clinical Microbiology ◽

10.1128/jcm.23.4.797-799.1986 ◽

1986 ◽

Vol 23 (4) ◽

pp. 797-799 ◽

Cited By ~ 19

Author(s):

P S Diegutis ◽

E Keirnan ◽

L Burnett ◽

B N Nightingale ◽

Y E Cossart

Keyword(s):

Hepatitis B Virus ◽

Hepatitis B ◽

Surface Antigen ◽

False Positive ◽

Hepatitis B Surface Antigen ◽

Hepatitis B Virus Dna ◽

Dot Hybridization ◽

B Virus ◽

Positive Results ◽

Dna Dot Hybridization

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A Discrepancy in Superoxide Scavenging Activity between the ESR-Spin Trapping Method and the Luminol Chemiluminescence Method

Bioscience Biotechnology and Biochemistry ◽

10.1271/bbb.70011 ◽

2007 ◽

Vol 71 (6) ◽

pp. 1505-1513 ◽

Cited By ~ 4

Author(s):

Emiko SATO ◽

Yoshimi NIWANO ◽

Takayuki MOKUDAI ◽

Masahiro KOHNO ◽

Yukihiko MATSUYAMA ◽

...

Keyword(s):

Spin Trapping ◽

Scavenging Activity ◽

Chemiluminescence Method ◽

Superoxide Scavenging Activity ◽

Luminol Chemiluminescence ◽

Esr Spin Trapping ◽

Trapping Method

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A Sensitive Chemiluminescence Procedure for the Determination of Carbon Monoxide with Myoglobin—Luminol Chemiluminescence System

Applied Spectroscopy ◽

10.1366/000370207781393398 ◽

2007 ◽

Vol 61 (7) ◽

pp. 706-710 ◽

Cited By ~ 6

Author(s):

Xiaofeng Xie ◽

Xili He ◽

Zhenghua Song

Keyword(s):

Carbon Monoxide ◽

Injection Technique ◽

Chemiluminescence Method ◽

Chemiluminescence Intensity ◽

Whole Process ◽

Enhanced Chemiluminescence ◽

Luminol Chemiluminescence ◽

Relative Standard ◽

Artificial Water

A novel chemiluminescence method combined with the flow injection technique for the determination of carbon monoxide is presented in this paper. The chemiluminescence signal based on the reaction between myoglobin and luminol in an alkaline medium was remarkably enhanced by carbon monoxide. The enhanced chemiluminescence intensity was linear with carbon monoxide concentration in the range from 0.01 to 10.0 pmol·L−1, and the detection limit was 3 × 10−3 pmol·L−1 (3σ). The whole process, including sampling and washing, could be completed in 0.5 min with a relative standard deviation of less than 4.0%. The proposed method was applied successfully in the assay of carbon monoxide in human serum and artificial water samples without any pretreatment procedure.

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