scholarly journals Studies on surface structures of diatomaceous earth supports and packing materials for gas chromatograph using scanning electron microscopy

1970 ◽  
Vol 19 (7) ◽  
pp. 907-918 ◽  
Author(s):  
Nobuta HAYASHI ◽  
Shigeo INOUE ◽  
Keiji TAKEUCHI
Author(s):  
Jane A. Westfall ◽  
S. Yamataka ◽  
Paul D. Enos

Scanning electron microscopy (SEM) provides three dimensional details of external surface structures and supplements ultrastructural information provided by transmission electron microscopy (TEM). Animals composed of watery jellylike tissues such as hydras and other coelenterates have not been considered suitable for SEM studies because of the difficulty in preserving such organisms in a normal state. This study demonstrates 1) the successful use of SEM on such tissue, and 2) the unique arrangement of batteries of nematocysts within large epitheliomuscular cells on tentacles of Hydra littoralis.Whole specimens of Hydra were prepared for SEM (Figs. 1 and 2) by the fix, freeze-dry, coat technique of Small and Màrszalek. The specimens were fixed in osmium tetroxide and mercuric chloride, freeze-dried in vacuo on a prechilled 1 Kg brass block, and coated with gold-palladium. Tissues for TEM (Figs. 3 and 4) were fixed in glutaraldehyde followed by osmium tetroxide. Scanning micrographs were taken on a Cambridge Stereoscan Mark II A microscope at 10 KV and transmission micrographs were taken on an RCA EMU 3G microscope (Fig. 3) or on a Hitachi HU 11B microscope (Fig. 4).


Author(s):  
Д.А. Кочуев ◽  
К.С. Хорьков ◽  
А.С. Черников ◽  
Р.В. Чкалов ◽  
В.Г. Прокошев

The paper presents the results of processing titanium surface in n-hexane at various pressures by femtosecond laser radiation. The effect of laser-induced plasma on the nature of the formation of periodic surface structures in the pressure range from 6 mbar to 22 bar is considered. The study of the surface of the processed samples was carried out using scanning electron microscopy. The dependence of the period of obtained laser-induced periodic surface structures on the pressure in working vessel and the treatment regime is shown.


1991 ◽  
Vol 69 (10) ◽  
pp. 2271-2278 ◽  
Author(s):  
N. Brun ◽  
M. Colson ◽  
A. Perrin ◽  
B. Voirin

The effects of ageing on monoterpene composition in Mentha ×piperita have been studied by analyses of microsamples obtained by coupling a desorption–concentration–introduction technique with a gas chromatograph. The appearance and the development of the two types of glands of shoot meristem studied by scanning electron microscopy show that the capitate glands appear before the peltate glands. The chromatographic analyses of meristem and different primordial leaf pairs show that the monoterpenes are only detected if the leaf bears peltate glands and that the first cyclic compound accumulated is limonene. The biochemical variations of the reduction of menthone to menthol in the midstem leaves and of acetylation of menthol in the basal leaves start at the distal extremity of the leaf and progressively move towards the base. The maturation of the plant followed by the shift of the level of leaf in which menthone and menthol are present with the same percentage as a function of time is a linear phenomenon. Key words: peppermint, trichomes, monoterpenes, biochemical variations, ageing.


2000 ◽  
Vol 8 (9) ◽  
pp. 36-37
Author(s):  
Greg Strout

Diatoms make for beautiful specimens for both transmission and scanning electron microscopy. As well, they are studied by many people, and there is always a need for good diatom preparations for EM.I find that the diatoms in diatomaceous earth are usually broken, and like to prepare my own from fresh specimens. The materials needed for this are a plankton net, some potassium dichromate, and 30% hydrogen peroxide. Once the diatoms are collected (plankton net tows from shoreline, or wherever), they can be cleaned using the chemicals.


2001 ◽  
Vol 15 (2) ◽  
pp. 91-94 ◽  
Author(s):  
S. Sabri Uslu ◽  
Fikret İleri ◽  
Ahmet Köybaşıoğlu ◽  
Hamdi Çelik ◽  
Mustafa Sargon ◽  
...  

2020 ◽  
Vol 44 (6) ◽  
pp. 490-497
Author(s):  
Alyssa Cristina Oliveira FREITAS ◽  
Adriana Aparecida Sinopólis GIGLIOLLI ◽  
Ronaldo Roberto Tait CALEFFE ◽  
Helio CONTE

Sitophilus zeamais Motschulsky, 1855 (Coleoptera: Curculionidae) which develops inside the grain, is an aggressive pest of stored grains. Here, we evaluated the efficiency of diatomaceous earth (DE), Diatomita CI/325 CX, and dolomite (DOL), Dolomita Dr. Kanyo, against S. zeamais reared on corn under laboratory conditions and observed external alterations on treated insects using scanning electron microscopy (SEM). Insects treated with both DE and DOL were impregnated with particles on the body surface, and they showed 100% mortality after 10 days of exposure. Damages caused by inert dusts may have contributed to dehydration and desiccation, consequently, leading insects to death. Thus, diatomaceous and dolomite were effective against S. zeamais under laboratory conditions.


2002 ◽  
Vol 116 (6) ◽  
pp. 420-425 ◽  
Author(s):  
J. Skladzień ◽  
J. A. Litwin ◽  
M. Nowogrodzka-Zagórska ◽  
A. J. Miodoński

Surfaces of aural polyps collected from 30 patients were examined by scanning electron microscopy. In the polyps not associated with cholesteatoma, the epithelial lining showed individually variable metaplasia towards cuboidal ‘cobblestone’-type and squamous epithelium covered with microvilli of various shapes and sizes. Squamous epithelium was present on the surface of all polyps with underlying cholesteatoma, with superficial cells possessing elongated microvilli, microplicae of different sizes, grooves and pits. Such surface structures reflect different stages of the keratinization process that seems to becharacteristic for the epithelial lining of polyps with underlying cholesteatoma. Incomplete epithelium accompanied by granulation tissue was found in several polyps; in two cholesteatoma-associated polyps plate-likecholesterol crystals were observed.


Sign in / Sign up

Export Citation Format

Share Document