scholarly journals Biodecolorization of azo dye mixture (Remazol Brilliant Violet 5R and Reactive Red 120) by indigenous bacterial consortium isolated from dye contaminated soil

Author(s):  
Ramaswamy, R. ◽  
Sundaravadivel, K. ◽  
Prakash, S. ◽  
Mohan, A.
2021 ◽  
Author(s):  
R Rajendran ◽  
S Kiruthika ◽  
P Saranya ◽  
Arunkumar Mohan ◽  
C V Vaishali

Abstract Discharge of the untreated wastewater containing dyestuff into the surrounding aquatic environment is of significant environmental concern. These dying effluents not only change the color of water bodies but also has many unfavorable conditions and release toxic by-products, which are mutagenic, carcinogenic, and hazardous to different life forms. The present study investigated the biodegradation and removal of dye mixture (Remazol Brilliant violet 5R and Reactive Red 120) using a new bacterial consortium isolated from dye contaminated soil. Among the total 15 isolates screened, the two most efficient bacterial species (SS07 and SS09) were selected and identified as Enterobacter cloacae (MT573884) and Achromobacter pulmonis (MT573885) through biochemical assays and 16S rRNA gene sequencing. The removal efficiency of dye mixture by Enterobacter cloacae and Achromobacter pulmonis at an initial concentration of 100 mg L− 1 was 82.78 and 84.96%, discretely. The bacterial consortium was developed using selected isolates, and the optimum conditions for the removal of dyes were investigated by studying the effects of pH, temperature, carbon and nitrogen sources, dye concentration, and inoculum size. The maximum decolorization efficiency was achieved at pH, 7; temperature, 37°C; dye concentration, 100 ppm; and initial inoculum concentration, 0.5 ml, respectively. Mannitol and Ammonium sulfate was identified as the most suitable carbon and nitrogen sources for better bacterial growth and decolorization. The maximum removal efficiency of 91.3% achieved at the optimal conditions after 72 h of incubation. Decolorization of azo dyestuff by the developed microbial consortia conforms to the zero-order reaction kinetics model. Consortia of Enterobacter cloacae and Achromobacter pulmonis was established as an effective decolorizer for the Remazol Brilliant violet 5R and Reactive Red 120 dye mixture with > 90% color removal.


Author(s):  
Motharasan Manogaran ◽  
Nur Adeela Yasid ◽  
Ahmad Razi Othman ◽  
Baskaran Gunasekaran ◽  
Mohd Izuan Effendi Halmi ◽  
...  

The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of Pseudomonas aeruginosa strain MM01, Enterobacter sp. strain MM05 and Serratia marcescens strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean’s seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.


2020 ◽  
Vol 31 (15) ◽  
pp. 12178-12190
Author(s):  
E. G. Villabona-Leal ◽  
Alondra G. Escobar-Villanueva ◽  
Víctor M. Ovando-Medina ◽  
Erick Balam Pérez-Pérez ◽  
Paola E. Díaz-Flores ◽  
...  

2021 ◽  
Vol 326 ◽  
pp. 124749
Author(s):  
Fang Tian ◽  
Yongbo Wang ◽  
Guang Guo ◽  
Keqiang Ding ◽  
Feng Yang ◽  
...  

Author(s):  
Carolina Rosai Mendes ◽  
Guilherme Dilarri ◽  
Paulo Renato Matos Lopes ◽  
Ederio Dino Bidoia ◽  
Renato Nallin Montagnolli
Keyword(s):  

2019 ◽  
Vol 9 (22) ◽  
pp. 4757 ◽  
Author(s):  
Mikel Anza ◽  
Oihane Salazar ◽  
Lur Epelde ◽  
José María Becerril ◽  
Itziar Alkorta ◽  
...  

Here, we aimed to bioremediate organically contaminated soil with Brassica napus and a bacterial consortium. The bioaugmentation consortium consisted of four endophyte strains that showed plant growth-promoting traits (three Pseudomonas and one Microbacterium) plus three strains with the capacity to degrade organic compounds (Burkholderia xenovorans LB400, Paenibacillus sp. and Lysinibacillus sp.). The organically contaminated soil was supplemented with rhamnolipid biosurfactant and sodium dodecyl benzenesulfonate to increase the degradability of the sorbed contaminants. Soils were treated with organic amendments (composted horse manure vs. dried cow slurry) to promote plant growth and stimulate soil microbial activity. Apart from quantification of the expected decrease in contaminant concentrations (total petroleum hydrocarbons, polycyclic aromatic hydrocarbons), the effectiveness of our approach was assessed in terms of the recovery of soil health, as reflected by the values of different microbial indicators of soil health. Although the applied treatments did not achieve a significant decrease in contaminant concentrations, a significant improvement of soil health was observed in our amended soils (especially in soils amended with dried cow slurry), pointing out a not-so-uncommon situation in which remediation efforts fail from the point of view of the reduction in contaminant concentrations while succeeding to recover soil health.


2020 ◽  
Vol 204 ◽  
pp. 111073 ◽  
Author(s):  
Guang Guo ◽  
Jiuxiao Hao ◽  
Fang Tian ◽  
Chong Liu ◽  
Keqiang Ding ◽  
...  
Keyword(s):  
Azo Dye ◽  

2015 ◽  
Vol 754-755 ◽  
pp. 671-675
Author(s):  
Muhammad Ridwan Fahmi ◽  
M.N. Nasruddin ◽  
Che Zulzikrami Azner Abidin ◽  
M. Ali Umi Fazara

In this study the performance of regenerated spent carbon for azo dye removal was evaluated in batch and continuous column. The spent carbon was generated by thermal method through pyrolysis process and chemical method by using NaOH 6.0 M solution. Reactive Red 120 (RR120) was selected as a model of azo dye due to its common application in the industries. The regeneration of spent granular activated carbon (GAC) by pyrolysis could produce adsorbent that has the adsorption capacity closer to new GAC. The result indicated that paralysed GAC could potentially apply to replace new GAC for RR120 adsorption. In addition, the continuous adsorption operation in mini column test confirmed that the order of adsorption capacity of each GAC is as follows: new GAC, pirolysed GAC, chemical treated GAC and spent GAC.


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