Biodecolorization of Azo Dye Mixture (Remazol Brilliant Violet 5r and Reactive Red 120) by Indigenous Bacterial Consortium Obtained From Dye Contaminated Soil

Abstract Discharge of the untreated wastewater containing dyestuff into the surrounding aquatic environment is of significant environmental concern. These dying effluents not only change the color of water bodies but also has many unfavorable conditions and release toxic by-products, which are mutagenic, carcinogenic, and hazardous to different life forms. The present study investigated the biodegradation and removal of dye mixture (Remazol Brilliant violet 5R and Reactive Red 120) using a new bacterial consortium isolated from dye contaminated soil. Among the total 15 isolates screened, the two most efficient bacterial species (SS07 and SS09) were selected and identified as Enterobacter cloacae (MT573884) and Achromobacter pulmonis (MT573885) through biochemical assays and 16S rRNA gene sequencing. The removal efficiency of dye mixture by Enterobacter cloacae and Achromobacter pulmonis at an initial concentration of 100 mg L− 1 was 82.78 and 84.96%, discretely. The bacterial consortium was developed using selected isolates, and the optimum conditions for the removal of dyes were investigated by studying the effects of pH, temperature, carbon and nitrogen sources, dye concentration, and inoculum size. The maximum decolorization efficiency was achieved at pH, 7; temperature, 37°C; dye concentration, 100 ppm; and initial inoculum concentration, 0.5 ml, respectively. Mannitol and Ammonium sulfate was identified as the most suitable carbon and nitrogen sources for better bacterial growth and decolorization. The maximum removal efficiency of 91.3% achieved at the optimal conditions after 72 h of incubation. Decolorization of azo dyestuff by the developed microbial consortia conforms to the zero-order reaction kinetics model. Consortia of Enterobacter cloacae and Achromobacter pulmonis was established as an effective decolorizer for the Remazol Brilliant violet 5R and Reactive Red 120 dye mixture with > 90% color removal.

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Carbon and Nitrogen Sources for Lipase Production ofMicrococcussp. Isolated from Palm Oil Mill Effluent-Contaminated Soil

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/217/1/012029 ◽

2019 ◽

Vol 217 ◽

pp. 012029 ◽

Cited By ~ 1

Author(s):

S. Sumarsih ◽

S. Hadi ◽

D.G.T. Andini ◽

F.K. Nafsihana

Keyword(s):

Palm Oil ◽

Contaminated Soil ◽

Nitrogen Sources ◽

Lipase Production ◽

Palm Oil Mill Effluent ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Palm Oil Mill

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Biodegradation of alachlor in liquid and soil cultures under variable carbon and nitrogen sources by bacterial consortium isolated from corn field soil

Iranian Journal of Environmental Health Science & Engineering ◽

10.1186/1735-2746-10-21 ◽

2013 ◽

Vol 10 (1) ◽

Cited By ~ 16

Author(s):

Mansooreh Dehghani ◽

Simin Nasseri ◽

Zahra Zamanian

Keyword(s):

Nitrogen Sources ◽

Bacterial Consortium ◽

Field Soil ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Corn Field

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Facile Degradation of Polyvinyl alcohol (PVA) by a novel bacterial strain Enterobacter cloacae VVD-MBB8 isolated from Gulf of Mannar, Tamil Nadu

10.31219/osf.io/ew2sd ◽

2020 ◽

Author(s):

Prakash Shyam Karuppiah ◽

Rajkumar Prabhakaran ◽

Ramya Venkatesan ◽

Sivabalan Sivasamy ◽

L.R.Monisha Miriam

Keyword(s):

Polyvinyl Alcohol ◽

Tamil Nadu ◽

Culture Medium ◽

Nitrogen Sources ◽

Enterobacter Cloacae ◽

Industrial Applications ◽

Gulf Of Mannar ◽

Rrna Gene ◽

Carbon And Nitrogen ◽

Distance Analysis

Polyvinyl alcohol (PVA) is a synthetic polymer, widely used for industrial applications, and its contamination related to the ecosystem concerns an increasingly serious problem. In the present study, we account a novel, potent PVA degrader, VVD-MBB8, isolated from the Gulf of Mannar. The VVD-MBB8 was identified as Enterobacter cloacae based on the molecular analysis using 16S rRNA gene sequence, biochemical and evolutionary distance analysis. This is the first report for the PVA degrader from the Enterobacter genus. The results showed 83% of the PVA present in the culture medium was degraded by Enterobacter cloacae VVD-MBB8 after 48 hrs of incubation with 30⁰C at the agitation of 145rpm. The results obtained from the FTIR showed notable differences in the PVA degradation stretches (3000 to 3300cm-1, 2500 to 3000cm-1) at 24 and 48 hrs compared with control (before degradation of PVA). The effects of carbon and nitrogen sources for PVA degradation were identified and the results revealed nitrogen sources have a significant increase in PVA degrading enzyme production and the enzyme activity was 83 U/ml.

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Study of the Bioremediation of Atrazine under Variable Carbon and Nitrogen Sources by Mixed Bacterial Consortium Isolated from Corn Field Soil in Fars Province of Iran

Journal of Environmental and Public Health ◽

10.1155/2013/973165 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 9

Author(s):

Mansooreh Dehghani ◽

Simin Nasseri ◽

Hassan Hashemi

Keyword(s):

Nitrogen Sources ◽

Bacterial Consortium ◽

Fars Province ◽

Field Soil ◽

Biodegradation Rate ◽

Soil Culture ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Corn Field ◽

Mixed Bacterial Consortium

Atrazine herbicide that is widely used in corn production is frequently detected in water resources. The main objectives of this research were focused on assessing the effects of carbon and nitrogen sources on atrazine biodegradation by mixed bacterial consortium and by evaluating the feasibility of using mixed bacterial consortium in soil culture. Shiraz corn field soil with a long history of atrazine application has been explored for their potential of atrazine biodegradation. The influence of different carbon compounds and the effect of nitrogen sources and a different pH (5.5–8.5) on atrazine removal efficiency by mixed bacterial consortium in liquid culture were investigated. Sodium citrate and sucrose had the highest atrazine biodegradation rate (87.22%) among different carbon sources. Atrazine biodegradation rate decreased more quickly by the addition of urea (26.76%) compared to ammonium nitrate. Based on the data obtained in this study, pH of 7.0 is optimum for atrazine biodegradation. After 30 days of incubation, the percent of atrazine reduction rates were significantly enhanced in the inoculated soils (60.5%) as compared to uninoculated control soils (12%) at the soil moisture content of 25%. In conclusion, bioaugmentation of soil with mixed bacterial consortium may enhance the rate of atrazine degradation in a highly polluted soil.

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Improved Biosurfactant Production by Enterobacter cloacae B14, Stability Studies, and its Antimicrobial Activity

Polish Journal of Microbiology ◽

10.33073/pjm-2020-030 ◽

2020 ◽

Vol 69 (3) ◽

pp. 273-282

Author(s):

JINDARAT EKPRASERT ◽

SASIWIMON KANAKAI ◽

SULADDA YOSPRASONG

Keyword(s):

Antimicrobial Activity ◽

Nitrogen Sources ◽

Enterobacter Cloacae ◽

Diffusion Method ◽

Infrared Spectrometry ◽

Carbon And Nitrogen Sources ◽

Gram Positive ◽

Carbon And Nitrogen ◽

Wide Range ◽

Layer Chromatography

This work aimed to optimize carbon and nitrogen sources for the growth of Enterobacter cloacae B14 and its biosurfactant (BS) production via One-Variable-At-a-Time (OVAT) method. The BS stability under a range of pH and temperatures was assessed. Antimicrobial activity against Gram-positive and Gram-negative pathogens was determined by the agar well diffusion method. The results showed that the optimum carbon and nitrogen sources for BS production were maltose and yeast extract, respectively, with a maximum BS yield of (39.8 ± 5.2) mg BS/g biomass. The highest emulsification activity (E24) was 79%, which is significantly higher than in the previous studies. We found that B14 BS can withstand a wide range of pH values from 2 to10. It could also function under a range of temperatures from 30–37°C. Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectrometry (FTIR) analysis confirmed that B14 BS is a glycolipid-like compound, which is rarely found in Enterobacter spp. Cell-free broth showed inhibition against various pathogens, preferable to Gram-positive ones. It had better antimicrobial activity against Bacillus subtilis than a commonly-used antibiotic, tetracycline. Furthermore, B14 broth could inhibit the growth of a tetracycline-resistant Serratia marcescens. Our results showed promising B14 BS applications not only for bioremediation but also for the production of antimicrobial products.

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Identification of mucin degraders of the human gut microbiota

Scientific Reports ◽

10.1038/s41598-021-90553-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Stefano Raimondi ◽

Eliana Musmeci ◽

Francesco Candeliere ◽

Alberto Amaretti ◽

Maddalena Rossi

Keyword(s):

Inflammatory Responses ◽

Nitrogen Sources ◽

Gene Profiling ◽

Rrna Gene ◽

Human Gut ◽

Carbon And Nitrogen Sources ◽

E Coli ◽

Carbon And Nitrogen ◽

Before And After ◽

Gut Mucosa

AbstractMucins are large glycoproteins consisting of approximately 80% of hetero-oligosaccharides. Gut mucin degraders of healthy subjects were investigated, through a culture dependent and independent approach. The faeces of five healthy adults were subjected to three steps of anaerobic enrichment in a medium with sole mucins as carbon and nitrogen sources. The bacterial community was compared before and after the enrichment by 16S rRNA gene profiling. Bacteria capable of fermenting sugars, such as Anaerotruncus, Holdemania, and Enterococcaceae likely took advantage of the carbohydrate chains. Escherichia coli and Enterobacteriaceae, Peptococcales, the Coriobacteriale Eggerthella, and a variety of Clostridia such as Oscillospiraceae, Anaerotruncus, and Lachnoclostridium, significantly increased and likely participated to the degradation of the protein backbone of mucin. The affinity of E. coli and Enterobacteriaceae for mucin may facilitate the access to the gut mucosa, promoting gut barrier damage and triggering systemic inflammatory responses. Only three species of strict anaerobes able to grow on mucin were isolated from the enrichments of five different microbiota: Clostridium disporicum, Clostridium tertium, and Paraclostridium benzoelyticum. The limited number of species isolated confirms that in the gut the degradation of these glycoproteins results from cooperation and cross-feeding among several species exhibiting different metabolic capabilities.

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Biodecolorization of azo dye mixture (Remazol Brilliant Violet 5R and Reactive Red 120) by indigenous bacterial consortium isolated from dye contaminated soil

Malaysian Journal of Microbiology ◽

10.21161/mjm.211202 ◽

2021 ◽

Author(s):

Ramaswamy, R. ◽

Sundaravadivel, K. ◽

Prakash, S. ◽

Mohan, A.

Keyword(s):

Contaminated Soil ◽

Azo Dye ◽

Bacterial Consortium ◽

Reactive Red 120 ◽

Remazol Brilliant Violet 5R

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Production, purification and characterization of thermostable alpha amylase from Bacillus subtilis Y25 isolated from decaying yam (Dioscorea rotundata) tuber

Notulae Scientia Biologicae ◽

10.15835/nsb12110521 ◽

2020 ◽

Vol 12 (1) ◽

pp. 154-171

Author(s):

Oluwatoyin M. ALADEJANA ◽

Olaoluwa OYEDEJI ◽

Olumide OMOBOYE ◽

Mufutau BAKARE

Keyword(s):

Bacillus Subtilis ◽

Metal Ion ◽

Specific Activity ◽

Nitrogen Sources ◽

16S Rrna Gene Sequencing ◽

Exchange Chromatography ◽

Rrna Gene ◽

Carbon And Nitrogen Sources ◽

Carbon And Nitrogen ◽

Amylase Production

Amylases have wide biotechnological potentials for applications in various industries. An α-amylase-producing bacterium was isolated from deteriorating yam tubers. Molecular characterization using the 16S rRNA gene sequencing was used to confirm the identity of the bacterium as Bacillus subtilis Y25. The effect of some cultural and nutritional factors such as pH, temperature, carbon and nitrogen sources on α-amylase production from the bacterium was determined. Maximum α-amylase production was observed using starch and peptone as carbon and nitrogen sources, respectively, with an initial medium pH of 8.0 and incubation at 45 °C for 36 h. The enzyme was purified by ion exchange chromatography on CM Sepharose CL-6B. The kinetic parameters Km and Vmax of the enzyme, as well as the effect of pH, temperature, metal ions and ethylenediaminetetra acetic acid (EDTA) on the activity of the purified enzyme were studied. The specific activity of the partially purified enzyme was determined to be 15.21 Units/mg protein with a purification fold of 3.80. The molecular weight of the purified enzyme was estimated to be 58.0 kDa. The Vmax and Km values obtained with soluble starch for Bacillus subtilis Y25 α-amylase were 314.10 ± 23.30 Units/mg protein and 53.98 ± 12.03 mg/ml, respectively. The enzyme exhibited optimum activity at a temperature of 60 °C and pH 8.0. The metal ion Ca2+ had no effect on the enzyme at 20 mM concentration, whereas Na+ and Mg2+, as well as EDTA inhibited the enzyme at the same concentration. The characteristics of the α-amylase from Bacillus subtilis Y25 revealed it to be a thermostable and an alkaline metalloenzyme with potential for applications in the detergent and saccharification industries.

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