Vitamin D status and its relation with insulin resistance and VDR-FokI polymorphism in Iranian non-melanoma skin cancer (NMSC) patients: a case-control study

Abstract Background: Sunlight exposure, the main source of endogenous vitamin D synthesis, may increase the risk of non-melanoma skin cancer (NMSC) development. Vitamin D receptor (VDR) polymorphisms are associated with 25(OH)D levels, cancer development and insulin resistance. This study was aimed to examine the associations among vitamin D status, VDR FokI polymorphism, insulin resistance and NMSC. Methods: This case-control study included 73 diagnosed cases of NMSC and 72 healthy controls from dermatology clinics at Razi Hospital, Tehran, Iran. A questionnaire was used to assess sunlight exposure. The extracted DNA from whole blood samples were genotyped. Fasting serum 25-hydroxyvitaminD (25(OH)D)), lipid profile, glucose, and insulin were measured. To evaluate insulin resistance, HOMA-IR formula was used. Results: We found a significant higher duration of cumulative sunlight exposure in cases compared with controls (p<0.001). However, 25(OH)D concentrations were not significantly different between cases and controls (30±15 vs. 29±15 ng/mL, p=0.78). Higher levels of insulin (p = 0.004) and HOMA-IR score (p= 0.019) were observed in Ff and ff genotype of FokI. We did not observe any significant increased risk of NMSC due to f allele, as compared with FF (OR =2.33, 95% CI 0.81-6.75, p=0.12). The components of lipid profile, fasting serum glucose, iPTH and anthropometric measures did not differ significantly across VDR genotypes. Conclusion: In conclusion, sunlight exposure was associated with NMSC risk. VDR FokI polymorphisms appears to influence insulin resistance in the NMSC patients. Keywords: sun exposure; non-melanoma skin cancer; polymorphisms; insulin resistance

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Vitamin D status and its relation with insulin resistance and VDR-FokI polymorphism in Iranian non-melanoma skin cancer (NMSC) patients: a case-control study

10.21203/rs.2.10291/v1 ◽

2019 ◽

Author(s):

Fatemeh Rezaiian ◽

Sayed Hossein Davoodi ◽

Bahareh Nikooyeh ◽

Amir Houshang Ehsani ◽

Ali Kalayi ◽

...

Keyword(s):

Insulin Resistance ◽

Vitamin D ◽

Case Control Study ◽

Case Control ◽

Sunlight Exposure ◽

Vitamin D Status ◽

Fasting Serum ◽

Non Melanoma Skin Cancer ◽

Control Study ◽

Melanoma Skin

Abstract Background Sunlight exposure, the main source of endogenous vitamin D synthesis, may increase the risk of non-melanoma skin cancer (NMSC) development. Vitamin D receptor (VDR) polymorphisms are associated with 25(OH)D levels, cancer development and insulin resistance. Objective This study aimed to examine the associations among vitamin D status, VDR FokI polymorphism, insulin resistance and NMSC. Methods This case-control study included 73 diagnosed cases of NMSC and 72 healthy controls from dermatology clinics at Razi Hospital, Tehran, Iran. A questionnaire was used to assess sunlight exposure. The extracted DNA from whole blood samples were genotyped. Fasting serum 25-hydroxyvitaminD (25(OH)D)), lipid profile, glucose, and insulin were measured. To evaluate insulin resistance, HOMA-IR formula was used. Results We found a significant higher duration of cumulative sunlight exposure in cases compared with controls (p<0.001). However, 25(OH)D concentrations were not significantly different between cases and controls (30±15 vs. 29±15 ng/mL, p=0.78). Higher levels of insulin (p = 0.004) and HOMA-IR score (p= 0.019) were observed in Ff and ff genotype of FokI. We did not observe any significant increased risk of NMSC due to f allele, as compared with FF (OR =2.33, 95% CI 0.81-6.75, p=0.12). The components of lipid profile, fasting serum glucose, iPTH and anthropometric measures did not differ significantly across VDR genotypes. Conclusion In conclusion, sunlight exposure was associated with NMSC risk. VDR FokI polymorphisms appears to influence insulin resistance in the NMSC patients.

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Vitamin D Receptor Polymorphisms and Non-Melanoma Skin Cancer Risk: A Case-Control Study

Journal of Clinical Medicine ◽

10.3390/jcm9123819 ◽

2020 ◽

Vol 9 (12) ◽

pp. 3819

Author(s):

Carolina Morgado-Águila ◽

Purificación Rey-Sánchez ◽

Guadalupe Gil-Fernández ◽

María Carmen Costa-Fernández ◽

Francisco José Rodríguez-Velasco

Keyword(s):

Vitamin D ◽

Skin Cancer ◽

Basal Cell ◽

Protective Factor ◽

Case Control Study ◽

Case Control ◽

Non Melanoma Skin Cancer ◽

Skin Cancers ◽

Control Study ◽

Melanoma Skin

Exposure to sunlight is the major source of vitamin D and the main environmental cause of non-melanocytic skin cancers. Vitamin D, partly mediated through the vitamin D receptor (VDR), has potential therapeutic applications in skin cancer. The aim of this study was to investigate the association of BsmI and ApaI VDR polymorphisms among patients with non-melanoma cancers and controls. An observational case-control study was conducted in a sample of 154 subjects. We observed no significant effects between these polymorphisms and skin cancer risk. When stratified for gender, GG and AG BsmI polymorphisms significantly increased the risk of basal cell carcinomas in males. In relation to ApaI, all three polymorphisms significantly increased the risk of basal cell carcinoma in males. When stratified for age, we found that being 70 years of age or younger was a protective factor against both skin cancers. Being a female and 70 years old or younger was a protective factor for basal cell carcinoma. A comparison of the frequencies of the VDR genotypes in patients older than 70 years vs. 70 years or younger also revealed age-dependent variations in patients with non-melanoma skin cancer. Our study suggests a role for VDR polymorphisms in non-melanoma skin cancer development.

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