scholarly journals Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

2020 ◽  
Author(s):  
Stefania Zanet ◽  
Elena Battisti ◽  
Paola Pepe ◽  
Lavinia Ciuca ◽  
Liliana Colombo ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Areas ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Specific Pcr ◽  
The Family ◽  
Borrelia Burgdorferi S.L ◽  
Privately Owned ◽  
Prevalent Species ◽  
Minimum Infection Rate ◽  
Theileria Spp

Abstract Background Ticks and tick-borne diseases are increasingly recognized as a cause of disease in dogs worldwide. The epidemiology of ticks and tick-transmitted protozoa and bacteria has changed due to the spread of ticks to urban and peri-urban areas and the movement of infected animals, posing new risks for animals and humans. This countrywide study reports information on distribution and prevalence of pathogens in ticks collected from privately-owned dogs in Italy. We analyzed 2681 Ixodidae ticks, collected from 1454 pet dogs from Italy. Specific PCR protocols were used to detect i) Piroplasms of the genera Babesia and Theileria , ii) Gram-negative cocci of the family Anaplasmataceae and iii) Borrelia burgdorferi sensu lato. Sequencing of positive amplicons allowed for species identification. Results Babesia / Theileria spp. DNA was detected in 435 homogeneous tick-pools (Minimum Infection Rate (MIR) = 27.6%; 95% confidence interval (CI) = 25.4-29.8%) with higher prevalence in Ixodes ricinus and Rhipicephalus sanguneus group. The zoonotic B. venatorum was the most prevalent species (MIR = 7.5%; 95% CI = 6.3-9.0%). Anaplasma and Ehrlichia species were detected in 165 tick-pools (MIR = 10.5%; 95% CI = 9.3-11.8%) and specifically, A. phagocytophilum was identified with MIR = 5.1% (95% CI = 4.1-6.3%). Borrelia burgdorferi s.l. and B. afzelii were detected with MIR = 0.4% (95% CI = 0.2-0.8%) and MIR = 0.3% (95% CI 0.1-0.7%) respectively. Conclusions Zoonotic pathogens B. venatorum and A. phagocytophilum were the most frequently detected in ticks collected from privately-owned dogs which might be used as markers of pathogens presence and distribution.

scholarly journals Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

2019 ◽  
Author(s):  
Stefania Zanet ◽  
Elena Battisti ◽  
Paola Pepe ◽  
Lavinia Ciuca ◽  
Liliana Colombo ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Areas ◽  
Gram Negative ◽  
Specific Pcr ◽  
The Family ◽  
Borrelia Burgdorferi S.L ◽  
Privately Owned ◽  
Prevalent Species ◽  
Minimum Infection Rate ◽  
Theileria Spp

Abstract Background Ticks and tick-borne diseases are increasingly recognized as a cause of disease in dogs worldwide. The epidemiology of ticks and tick-transmitted protozoa and bacteria has changed due to the spread of ticks to urban and peri-urban areas and the movement of infected animals, posing new risks for animals and humans. This countrywide study reports information on distribution and prevalence of pathogens in ticks collected from privately-owned dogs in Italy. We analyzed 2681 Ixodidae ticks, collected from 1454 pet dogs from Italy. Specific PCR protocols were used to detect i) Piroplasms of the genera Babesia and Theileria , ii) Gram-negative cocci of the family Anaplasmataceae and iii) Borrelia burgdorferi s.l. Sequencing of positive amplicons allowed for species identification.Results Babesia / Theileria spp. DNA was detected in 435 tick-pools (Minimum Infection Rate (MIR) = 27.57%; 95% confidence interval (CI) = 25.42-29.82%) with higher prevalence in Ixodes ricinus and Rhipicephalus sanguneus group. The zoonotic B. venatorum was the most prevalent species (MIR = 7.54%; 95% CI = 6.34-8.95%). Anaplasma and Ehrlichia species were detected in 165 tick-pools (MIR = 10.46%; 95% CI = 9.26-11.79%) and specifically, A. phagocytophilum was identified with MIR = 5.07% (95% CI = 4.09-6.27%). Borrelia burgdorferi s.l. and B. afzelii were detected with MIR = 0.38% (95% CI = 0.17-0.83%) and MIR = 0.25% (95% CI 0.1-0.65%) respectively.Conclusions Zoonotic pathogens were the most frequently detected in ticks collected from privately-owned dogs which might be used as markers of pathogens presence and distribution.

Human pathogenic borreliae in Ixodes ricinus ticks in natural and urban ecosystem (Czech Republic)

2014 ◽  
Vol 59 (4) ◽  
Author(s):  
Kristýna Venclíková ◽  
Lenka Betášová ◽  
Silvie Šikutová ◽  
Petra Jedličková ◽  
Zdeněk Hubálek ◽  
...  
Keyword(s):  
Czech Republic ◽  
Borrelia Burgdorferi ◽  
Ixodes Ricinus ◽  
Urban Areas ◽  
Urban Ecosystem ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Urban Park ◽  
Borrelia Burgdorferi S.L ◽  
Ixodes Ricinus Ticks ◽  
First Time

AbstractA total of 1279 field-collected Ixodes ricinus ticks were screened for Borrelia burgdorferi sensu lato (s.l.) in a natural and an urban ecosystem of Ostrava city (Czech Republic) by using molecular methods. Minimal prevalence rate for Borrelia burgdorferi s.l. in ticks for the urban park Bělský les was found to be 13.8% (17.6% in males, 17.8% in females and 11.7% in nymphs), similarly for the natural site Proskovice was minimal prevalence 15% (12.5% in males, 20% in females and 14.9% in nymphs). Six proven human pathogenic genomic species have been recorded in the study: B. afzelii, B. garinii, B. burgdorferi s.s., B. valaisiana, B. lusitaniae, and B. spielmanii. Emerging B. spielmanii was detected for the first time in Ixodes ricinus ticks in the region. Our results highlight the need for surveillance of zoonotic tick-borne pathogens even in urban areas.

scholarly journals SEROPREVALENCE OF MOSQUITO-BORN AND TICK-BORN MICROORGANISMS IN HUMAN POPULATION OF SOUTH BACKA DISTRICT

2016 ◽  
Vol 9 (1) ◽  
pp. 23-30 ◽  
Author(s):  
Ivana Hrnjaković Cvjetković ◽  
Tamaš Petrović ◽  
Dušan Petrić ◽  
Dejan Cvjetković ◽  
Gordana Kovačević ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Areas ◽  
Human Population ◽  
Chikungunya Virus ◽  
Bird Species ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Igg Antibodies ◽  
Public Attention ◽  
Usutu Virus ◽  
The Family

Chikungunya virus is an Arbo virus belonging to the family Togaviridae. In urban areas, antropophilic Aedes aegypti and Aedes albopictus mosquitoes are vectors for virus transmission to human population. Chikungunya virus has attracted the professional and scientifi c public attention in 2013 causing a massive outbreak on the American continent. In Europe, autochthonous transmissions of Chikungunya virus infections have beenrecorded in Italy in 2007 as well as in France in 2010 and 2014. Usutu virus is a RNA virus from the family Flaviviridae. Th e virus circulates in a transmission cycle between wild birds and Culex mosquitoes. The virus has been detected in numerous bird species across Europe. Manifestations recorded in humans include meningoencephalitis and skin rash. First human cases in Europe were recorded in immunocompromised individuals in Italy in 2009. Spirochete Borrelia burgdorferi sensu lato is transmitted to humans by ticks and causes Lyme disease, a multisystemic disease with dermatological, neurological, cardiological or articular manifestations. Ninety three persons interviewed about risk factors for vector-borne infections were examined. The examination was performed using commercial ELISA IgG for Chikungunya and Usutu virus and ELISA IgM and IgG test for Borrelia burgdorferi in line with manufacturer’s instructions (Euroimmun, Germany). Out of 93 examined individuals Usutu virus specific IgG antibodies were identified in 7.5% (7/93) persons. Th e results of ELISA IgG test for Chikungunya virus were negative in the majority of tested samples, whereas 7.5% (7/93) of samples revealed borderline result. In 9.7% (9/93) participants, antibodies against Borrelia burgdorferi were detected only by ELISA IgM test. Recent infection with Borrelia burgdorferi was confi rmed in 2.15% (2/93) individuals, whereas IgG antibodies against Borrelia burgdorferi were detected in only one participant. Th e obtained results indicated that Usutu virus is active in the territory of South Bačka District contrary to Chikungunya virus as well as that Borrelia burgdorferi is an important pathogen in the investigated region.

scholarly journals Genospecies Identification and Characterization of Lyme Disease Spirochetes of Genospecies Borrelia burgdorferi Sensu Lato Isolated from Rodents in Taiwan

1998 ◽  
Vol 36 (11) ◽  
pp. 3127-3132 ◽  
Author(s):  
Chien-Ming Shih ◽  
Han-Ming Chang ◽  
Show-Li Chen ◽  
Li-Lian Chao
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Pcr Primers ◽  
Sensu Stricto ◽  
Surface Proteins ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Pcr Analysis ◽  
Protein Profiles ◽  
Specific Pcr ◽  
Plasmid Profiles

Lyme disease spirochetes of the genospecies Borrelia burgdorferi sensu lato were identified and characterized for the first time in Taiwan. Seven isolates, designated TWKM1 to TWKM7, were purified from the ear tissues of three species of rodents captured from seven localities of Taiwan. The immunological characteristics of these Taiwan isolates were compared with those of other genospecies of Lyme disease spirochetes by analyzing the protein profiles and reactivities with B. burgdorferi-specific monoclonal antibodies (MAbs). The genospecies of these Taiwan isolates were also identified by the similarities in their plasmid profiles and differential reactivities with genospecies-specific PCR primers. Although two distinct protein profiles were observed among the seven Taiwan isolates, the MAb reactivities against the outer surface proteins of B. burgdorferi of all of these isolates were consistent with those of B. burgdorferi sensu lato. The similarities of the plasmid profiles also confirmed the identities of these Taiwan isolates. PCR analysis indicated that all of these Taiwan isolates were genetically related to the genospecies B. burgdorferi sensu stricto. These results demonstrate the first identification of Lyme disease spirochetes in Taiwan and also highlight the increasing demand for defining the reservoirs and vector ticks of B. burgdorferi. A serosurvey for Lyme disease infection in the human population of Taiwan may also be required.

scholarly journals Molecular Detection of Borrelia burgdorferi Sensu Lato and Anaplasma phagocytophilum in Ticks Collected from Dogs in Urban Areas of North-Eastern Poland

Pathogens ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 455
Author(s):  
Mirosław M. Michalski ◽  
Katarzyna Kubiak ◽  
Magdalena Szczotko ◽  
Marta Chajęcka ◽  
Małgorzata Dmitryjuk
Keyword(s):  
Borrelia Burgdorferi ◽  
Anaplasma Phagocytophilum ◽  
Urban Areas ◽  
Molecular Detection ◽  
Dermacentor Reticulatus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
16S Rna ◽  
North Eastern ◽  
The City ◽  
Rna Genes

From 2016 to 2018, ticks were collected from 272 dogs admitted to veterinary clinics in the city of Olsztyn (north-eastern Poland). Among 522 collected ticks, 423 were identified as Ixodes ricinus (413 females and 10 males) and 99 as Dermacentor reticulatus (62 females and 37 males). Non-engorged (86 individuals) and engorged (436 individuals) ticks were screened for the presence of Borrelia burgdorferi sensu lato and Anaplasma phagocytophilum DNA. Borrelia and A. phagocytophilum species detection was determined based on the sequence of the fla B and 16S RNA genes, respectively. DNA of B. burgdorferi s.l. was identified in 31.6% (165/522, 95% CI: 27.6–35.8%) of ticks (I. ricinus 151/423, 35.7%, 95% CI: 31.1–40.4%; D. reticulates 14/99, 14.1%, 95% CI: 7.9–22.6%). A. phagocytophilum was identified in 0.96% (5/522, 95% CI: 0.3–2.2%) of specimens. All positive samples were engorged I. ricinus females (5/402, 1.2%, 95% CI: 0.4–2.9%). In 85.4% (141/165, 95% CI: 79.1–90.4%) of Borrelia infected ticks, the DNA of one genospecies was revealed. The DNA of at least two different genospecies was detected in 14.5% of specimens (24/165, 95% CI: 9.5–20.8). The coexistence of B. burgdorferii s.l. and A. phagocytophilum was not detected.

Does more favourable handling of the cerebrospinal fluid increase the diagnostic sensitivity of Borrelia burgdorferi sensu lato-specific PCR in Lyme neuroborreliosis?

2017 ◽  
Vol 50 (4) ◽  
pp. 297-302 ◽  
Author(s):  
Kristine J. N. Forselv ◽  
Åslaug R. Lorentzen ◽  
Unn Ljøstad ◽  
Åse Mygland ◽  
Randi Eikeland ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Borrelia Burgdorferi ◽  
Lyme Neuroborreliosis ◽  
Diagnostic Sensitivity ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Specific Pcr

Identification and characterization of 31 isolates of Borrelia burgdorferi (Spirochaetales, Spirochaetaceae) obtained from various hosts and vectors using PCR-RFLP and SDS-PAGE analysis

2008 ◽  
Vol 53 (2) ◽  
Author(s):  
Alena Žákovská ◽  
Eva Janouškovcová ◽  
Kateřina Pejchalová ◽  
Jiří Halouzka ◽  
Miloš Dendis
Keyword(s):  
Czech Republic ◽  
Borrelia Burgdorferi ◽  
Etiologic Agent ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Wild Rodents ◽  
The Czech Republic ◽  
Sds Page ◽  
Pcr Rflp ◽  
Borrelia Burgdorferi S.L ◽  
Pcr Method

AbstractBorrelia burgdorferi sensu lato, the etiologic agent of Lyme borreliosis, circulates between ticks and vertebrate hosts. Two main genospecies typically occur in the Czech Republic Borrelia garinii and Borrelia afzelii, transmitted generally by Ixodes ricinus (L., 1758) ticks. The aim of our study was to identify spirochaete isolates focusing on Borrelia burgdorferi acquired from different sources: vectors (ticks), potential vectors (mosquitoes, small mites) and hosts (wild rodents). In the years 1996–2001 a total of 2398 ticks, 72 mites (from wild rodents), 2700 mosquito adults, 1798 mosquito larvae and organ parts (kidney and spleen) of 216 wild rodents were collected from seven localities in the Czech Republic. A total of 31 spirochaete strains were isolated: 13 strains from ticks, 1 strain from mite (Haemogamasus sp.), 15 strains from rodents, 1 strain from mosquito adults and 1 strain from mosquito larva. For the genospecies identification of these isolates PCR, PCR-RFLP was used and their characterization was also performed by SDS-PAGE. By nested PCR method all except one isolated strains were detected as Borrelia burgdorferi s.l. Following PCR-RFLP molecular analysis results, tick isolates were identified as B. garinii and B. afzelii, the strain isolated from the mite was identified as B. afzelii. This is the first isolated strain of B.b.s.l. from a different mite of infraorder Parasitiformes than tick. All of rodent isolates were identified as B. afzelii; mosquito adult isolate was identified as B. afzelii. Larval isolate from mosquito is spirochaete, but does not belong to Borrelia burgdorferi sensu lato group.

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