Heterogeneity of Borrelia burgdorferi sensu lato demonstrated by an ospA -type-specific PCR in synovial fluid from patients with Lyme arthritis

1998 ◽  
Vol 187 (2) ◽  
pp. 97-102 ◽  
Author(s):  
Vlad Vasiliu ◽  
Peter Herzer ◽  
Dieter Rössler ◽  
Gisela Lehnert ◽  
B. Wilske
Keyword(s):  
Synovial Fluid ◽  
Borrelia Burgdorferi ◽  
Lyme Arthritis ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Specific Pcr

scholarly journals Tick-borne pathogens in Ixodidae ticks collected from privately-owned dogs in Italy: a country-wide molecular survey

2020 ◽  
Author(s):  
Stefania Zanet ◽  
Elena Battisti ◽  
Paola Pepe ◽  
Lavinia Ciuca ◽  
Liliana Colombo ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Urban Areas ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Specific Pcr ◽  
The Family ◽  
Borrelia Burgdorferi S.L ◽  
Privately Owned ◽  
Prevalent Species ◽  
Minimum Infection Rate ◽  
Theileria Spp

Abstract Background Ticks and tick-borne diseases are increasingly recognized as a cause of disease in dogs worldwide. The epidemiology of ticks and tick-transmitted protozoa and bacteria has changed due to the spread of ticks to urban and peri-urban areas and the movement of infected animals, posing new risks for animals and humans. This countrywide study reports information on distribution and prevalence of pathogens in ticks collected from privately-owned dogs in Italy. We analyzed 2681 Ixodidae ticks, collected from 1454 pet dogs from Italy. Specific PCR protocols were used to detect i) Piroplasms of the genera Babesia and Theileria , ii) Gram-negative cocci of the family Anaplasmataceae and iii) Borrelia burgdorferi sensu lato. Sequencing of positive amplicons allowed for species identification. Results Babesia / Theileria spp. DNA was detected in 435 homogeneous tick-pools (Minimum Infection Rate (MIR) = 27.6%; 95% confidence interval (CI) = 25.4-29.8%) with higher prevalence in Ixodes ricinus and Rhipicephalus sanguneus group. The zoonotic B. venatorum was the most prevalent species (MIR = 7.5%; 95% CI = 6.3-9.0%). Anaplasma and Ehrlichia species were detected in 165 tick-pools (MIR = 10.5%; 95% CI = 9.3-11.8%) and specifically, A. phagocytophilum was identified with MIR = 5.1% (95% CI = 4.1-6.3%). Borrelia burgdorferi s.l. and B. afzelii were detected with MIR = 0.4% (95% CI = 0.2-0.8%) and MIR = 0.3% (95% CI 0.1-0.7%) respectively. Conclusions Zoonotic pathogens B. venatorum and A. phagocytophilum were the most frequently detected in ticks collected from privately-owned dogs which might be used as markers of pathogens presence and distribution.

scholarly journals Genospecies Identification and Characterization of Lyme Disease Spirochetes of Genospecies Borrelia burgdorferi Sensu Lato Isolated from Rodents in Taiwan

1998 ◽  
Vol 36 (11) ◽  
pp. 3127-3132 ◽  
Author(s):  
Chien-Ming Shih ◽  
Han-Ming Chang ◽  
Show-Li Chen ◽  
Li-Lian Chao
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Pcr Primers ◽  
Sensu Stricto ◽  
Surface Proteins ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Pcr Analysis ◽  
Protein Profiles ◽  
Specific Pcr ◽  
Plasmid Profiles

Lyme disease spirochetes of the genospecies Borrelia burgdorferi sensu lato were identified and characterized for the first time in Taiwan. Seven isolates, designated TWKM1 to TWKM7, were purified from the ear tissues of three species of rodents captured from seven localities of Taiwan. The immunological characteristics of these Taiwan isolates were compared with those of other genospecies of Lyme disease spirochetes by analyzing the protein profiles and reactivities with B. burgdorferi-specific monoclonal antibodies (MAbs). The genospecies of these Taiwan isolates were also identified by the similarities in their plasmid profiles and differential reactivities with genospecies-specific PCR primers. Although two distinct protein profiles were observed among the seven Taiwan isolates, the MAb reactivities against the outer surface proteins of B. burgdorferi of all of these isolates were consistent with those of B. burgdorferi sensu lato. The similarities of the plasmid profiles also confirmed the identities of these Taiwan isolates. PCR analysis indicated that all of these Taiwan isolates were genetically related to the genospecies B. burgdorferi sensu stricto. These results demonstrate the first identification of Lyme disease spirochetes in Taiwan and also highlight the increasing demand for defining the reservoirs and vector ticks of B. burgdorferi. A serosurvey for Lyme disease infection in the human population of Taiwan may also be required.

scholarly journals Induction of Host Matrix Metalloproteinases by Borrelia burgdorferi Differs in Human and Murine Lyme Arthritis

2005 ◽  
Vol 73 (1) ◽  
pp. 126-134 ◽  
Author(s):  
Aruna K. Behera ◽  
Ethan Hildebrand ◽  
Joanna Scagliotti ◽  
Allen C. Steere ◽  
Linden T. Hu
Keyword(s):  
Synovial Fluid ◽  
Matrix Metalloproteinases ◽  
Borrelia Burgdorferi ◽  
Articular Surface ◽  
Expression Patterns ◽  
Gene Array ◽  
Lyme Arthritis ◽  
Cartilage Tissue ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tissue Inhibitor

ABSTRACT Matrix metalloproteinases (MMPs) are induced from host tissues in response to Borrelia burgdorferi. Upregulation of MMPs may play a role in the dissemination of the organism through extracellular matrix tissues, but it can also result in destructive pathology. Although mice are a well-accepted model for Lyme arthritis, there are significant differences compared to human disease. We sought to determine whether MMP expression could account for some of these differences. MMP expression patterns following B. burgdorferi infection were analyzed in primary human chondrocytes, synovial fluid samples from patients with Lyme arthritis, and cartilage tissue from Lyme arthritis-susceptible and -resistant mice by using a gene array, real-time PCR, an enzyme-linked immunosorbent assay, and immunohistochemistry. B. burgdorferi infection significantly induced transcription of MMP-1, -3, -13, and -19 from primary human chondrocyte cells. Transcription of MMP-10 and tissue inhibitor of metalloprotease 1 was increased with B. burgdorferi infection, but protein expression was only minimally increased. The synovial fluid levels of MMPs from patients with high and low spirochete burdens were consistent with results seen in the in vitro studies. B. burgdorferi-susceptible C3H/HeN mice infected with B. burgdorferi showed induction of MMP-3 and MMP-19 but no other MMP or tissue inhibitor of metalloprotease. As determined by immunohistochemistry, MMP-3 expression was increased only in chondrocytes near the articular surface. The levels of MMPs were significantly lower in the more Lyme arthritis-resistant BALB/c and C57BL/6 mice. Differences between human and murine Lyme arthritis may be related to the lack of induction of collagenases, such MMP-1 and MMP-13, in mouse joints.

Detection of Borrelia burgdorferi DNA by Polymerase Chain Reaction in Synovial Fluid from Patients with Lyme Arthritis

1994 ◽  
Vol 330 (4) ◽  
pp. 229-234 ◽  
Author(s):  
James J. Nocton ◽  
Frank Dressler ◽  
Barbara J. Rutledge ◽  
Paul N. Rys ◽  
David H. Persing ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Synovial Fluid ◽  
Borrelia Burgdorferi ◽  
Lyme Arthritis ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Misdiagnosis of Late-Onset Lyme Arthritis by Inappropriate Use of Borrelia burgdorferi Immunoblot Testing with Synovial Fluid

2012 ◽  
Vol 19 (11) ◽  
pp. 1806-1809 ◽  
Author(s):  
Sam S. Barclay ◽  
Michael T. Melia ◽  
Paul G. Auwaerter
Keyword(s):  
Lyme Disease ◽  
Synovial Fluid ◽  
Borrelia Burgdorferi ◽  
Late Onset ◽  
Medical Center ◽  
Academic Medical Center ◽  
Primary Objective ◽  
Lyme Arthritis ◽  
Serum Samples ◽  
Content Type

ABSTRACTThe primary objective of this study was to determine whether patients with putative late-onset Lyme arthritis based upon synovial fluidBorrelia burgdorferiIgM and IgG immunoblot testing offered by commercial laboratories satisfied conventional criteria for the diagnosis of Lyme arthritis. Secondary objectives included assessing the prior duration and responsiveness of associated antibiotic therapy. We conducted a retrospective analysis of 11 patients referred to an academic medical center infectious disease clinic during the years 2007 to 2009 with a diagnosis of Lyme disease based upon previously obtained synovial fluidB. burgdorferiimmunoblot testing. Ten of the 11 (91%) patients with a diagnosis of late-onset Lyme arthritis based upon interpretation of synovial fluidB. burgdorferiimmunoblot testing were seronegative and did not satisfy published criteria for the diagnosis of late-onset Lyme arthritis. None of the 10 patients had a clinical response to previously received antibiotics despite an average course of 72 days. Diagnosis of Lyme arthritis should not be based on synovial fluidB. burgdorferiimmunoblot testing. This unvalidated test does not appear useful for the diagnosis of Lyme disease, and this study reinforces the longstanding recommendation to useB. burgdorferiimmunoblot testing only on serum samples and not other body fluids. Erroneous interpretations of “positive” synovial fluid immunoblots may lead to inappropriate antibiotic courses and delays in diagnosis of other joint diseases.

Lyme-Arthritis bei Kindern

2011 ◽  
Vol 31 (02) ◽  
pp. 129-134 ◽  
Author(s):  
H.-I. Huppertz ◽  
F. Dressler
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Arthritis ◽  
Borrelia Burgdorferi Sensu Lato

ZusammenfassungDie Lyme-Arthritis ist durch die Infektion mit Borrelia burgdorferi sensu lato verursacht. In dieser Arbeit wird der aktuelle Wissensstand zu Epidemiologie, Mikrobiologie, Pathogenese sowie zu den klinischen Manifestationen und deren Diagnose, Behandlung und Prognose dargestellt, besonders bezogen auf das Kindes- und Jugendalter.

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