scholarly journals The expressions of tooth eruption relevant genes are different in incisors and molars dental follicle cells in rat: an in vitro study

2019 ◽  
Author(s):  
Mengting He ◽  
Xiaomeng Dong ◽  
Peiqi Wang ◽  
Zichao Xiang ◽  
Jiangyue Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Expression Patterns ◽  
In Vitro Study ◽  
Tooth Eruption ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Real Time Qpcr

Abstract Background The incisors and molars showed different patterns of tooth eruption in rodents and the dental follicle cells play key roles in tooth eruption. Little is known about the differences in incisors and molars dental follicle cells during tooth eruption in rodents. The purpose of this study was to investigate the differences between incisor dental follicle cells and molar dental follicle cells during tooth eruption in rat.Methods Incisor dental follicle cells and molar dental follicle cells were obtained as previously described. Immunofluorescence was used to identify the cells. Gene expression was measured by real-time qPCR and western blot.Results Compared with molar dental follicle cells, the incisor dental follicle cells showed higher expression of OPG, BMP-2 and BMP-3. The molar dental follicle cells showed higher expression of MCP-1 and RANKL.Conclusions The expression patterns of genes related to tooth eruption were different in incisors and molars dental follicle cells in rat.

Gene expression profiles of dental follicle cells before and after osteogenic differentiation in vitro

2009 ◽  
Vol 13 (4) ◽  
pp. 383-391 ◽  
Author(s):  
Christian Morsczeck ◽  
Gottfried Schmalz ◽  
Torsten Eugen Reichert ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
...  
Keyword(s):  
Gene Expression ◽  
Osteogenic Differentiation ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Before And After

scholarly journals ZipSeq : Barcoding for Real-time Mapping of Single Cell Transcriptomes

2020 ◽  
Author(s):  
Kenneth H. Hu ◽  
John P. Eichorst ◽  
Chris S. McGinnis ◽  
David M. Patterson ◽  
Eric D. Chow ◽  
...  
Keyword(s):  
Gene Expression ◽  
Single Cell ◽  
Real Time ◽  
Dimensional Space ◽  
Single Cells ◽  
Expression Patterns ◽  
Live Cells ◽  
Glass Substrates ◽  
Complete Mapping

ABSTRACTSpatial transcriptomics seeks to integrate single-cell transcriptomic data within the 3-dimensional space of multicellular biology. Current methods use glass substrates pre-seeded with matrices of barcodes or fluorescence hybridization of a limited number of probes. We developed an alternative approach, called ‘ZipSeq’, that uses patterned illumination and photocaged oligonucleotides to serially print barcodes (Zipcodes) onto live cells within intact tissues, in real-time and with on-the-fly selection of patterns. Using ZipSeq, we mapped gene expression in three settings: in-vitro wound healing, live lymph node sections and in a live tumor microenvironment (TME). In all cases, we discovered new gene expression patterns associated with histological structures. In the TME, this demonstrated a trajectory of myeloid and T cell differentiation, from periphery inward. A variation of ZipSeq efficiently scales to the level of single cells, providing a pathway for complete mapping of live tissues, subsequent to real-time imaging or perturbation.

Comparison of human dental follicle cells (DFCs) and stem cells from human exfoliated deciduous teeth (SHED) after neural differentiation in vitro

2009 ◽  
Vol 14 (4) ◽  
pp. 433-440 ◽  
Author(s):  
Christian Morsczeck ◽  
Florian Völlner ◽  
Michael Saugspier ◽  
Caroline Brandl ◽  
Torsten Eugen Reichert ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Differentiation ◽  
Follicle Cells ◽  
Deciduous Teeth ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Human Exfoliated Deciduous Teeth

Secretion of CSF-1 and its inhibition in rat dental follicle cells, implications for tooth eruption

1998 ◽  
Vol 106 (3) ◽  
pp. 808-815 ◽  
Author(s):  
Robert L. Grier IV ◽  
Lihong Zhao ◽  
Charles E. Adams ◽  
Gary E. Wise
Keyword(s):  
Tooth Eruption ◽  
Follicle Cells ◽  
Dental Follicle ◽  
Dental Follicle Cells

Regulation of Osteoprotegerin Gene Expression in Dental Follicle Cells

2003 ◽  
Vol 82 (4) ◽  
pp. 298-302 ◽  
Author(s):  
G.E. Wise ◽  
Y. Ren ◽  
S. Yao
Keyword(s):  
Gene Expression ◽  
Steady State ◽  
Protein Kinase ◽  
Follicle Cells ◽  
Protein Kinase Activity ◽  
Mrna Levels ◽  
Dental Follicle ◽  
Dental Follicle Cells ◽  
Parathyroid Hormone Related Protein ◽  
Mandibular Molar

Colony-stimulating factor-one (CSF-1) and parathyroid-hormone-related protein (PTHrP) down-regulate osteoprotegerin (OPG) gene expression in the dental follicle of the rat first mandibular molar. To examine this regulation at the signal transduction level, we treated cultured dental follicle cells with either phorbolmyristate acetate (PMA) or dibutyryl cyclic AMP (dbcAMP) to activate either protein kinase C (PKC) or protein kinase A (PKA). Our results demonstrate that PMA up-regulates OPG gene expression and down-regulates the expression of CSF-1 and the PTHrP receptor (PTHrP-R). Conversely, dbcAMP down-regulates OPG expression and up-regulates CSF-1 and PTHrP-R expression. Immunostaining shows that PMA also increases the steady-state levels of protein. Thus, treatment with agents that affect protein kinase activity also enhance the steady-state mRNA and protein levels of OPG, as well as decreasing the mRNA levels of CSF-1 and PTHrP-R. The PKC-α isoform may be critical in OPG regulation because PKC-α gene expression is enhanced by PMA and reduced by either CSF-1 or PTHrP.

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