scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Respiratory Burst ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Group I ◽  
Experimental Group

Abstract Background The objective of this study was to evaluate the effect of HMB on the chemotactic activity, phagocytic activity and respiratory burst of peripheral blood granulocytes and monocytes in calves. Method The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB (Metabolic Technologies Inc. Ames, IA, USA) at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine: chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and respiratory burst (BURSTTEST® kit) of peripheral blood granulocytes and monocytes by flow cytometry. Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism and MFI. Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Respiratory Metabolism ◽  
Group I ◽  
Experimental Group

Abstract Background The objective of this study was to evaluate the effect of HMB on the chemotactic activity, phagocytic activity and respiratory metabolism of peripheral blood granulocytes and monocytes in calves.Method The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB (Metabolic Technologies Inc. Ames, IA, USA) at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine: chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and respiratory metabolism (BURSTTEST® kit) of peripheral blood granulocytes and monocytes by flow cytometry.Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism and MFI.Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Respiratory Burst ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Escherichia Coli Bacteria ◽  
Leucine Metabolite

Abstract Background A healthy immune system plays a particularly important role in newborns, including in calves that are far more susceptible to infections (viral, bacterial and other) than adult individuals. Therefore the objective of this study was to evaluate the effect of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and respiratory burst (BURSTTEST® kit) of peripheral blood granulocytes and monocytes in calves by flow cytometry. Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism and MFI. Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Newborn Calves ◽  
Leucine Metabolite ◽  
Positive Effect

Abstract Background A healthy immune system plays a particularly important role in newborns, including in calves that are far more susceptible to infections (viral, bacterial and other) than adult individuals. Therefore, the present study aimed to evaluate the influence of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and oxidative burst (BURSTTEST® kit) of monocytes and granulocytes in the peripheral blood of calves by flow cytometry.Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of monocytes and granulocytes stimulated with PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria, expressed as MFI values and the percentage of oxidative metabolism.Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Newborn Calves ◽  
Escherichia Coli Bacteria ◽  
Leucine Metabolite

Abstract Background A healthy immune system plays a particularly important role in newborns, including in calves that are far more susceptible to infections (viral, bacterial and other) than adult individuals. Therefore, the present study aimed to evaluate the influence of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and oxidative burst (BURSTTEST® kit) of monocytes and granulocytes in the peripheral blood of calves by flow cytometry. Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of monocytes and granulocytes stimulated with PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria, expressed as MFI values and the percentage of oxidative metabolism. Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The influence of dietary supplementation with the leucine metabolite β-hydroxy-β-methylbutyrate (HMB) on the chemotaxis, phagocytosis and respiratory burst of peripheral blood granulocytes and monocytes in calves

2020 ◽  
Author(s):  
Roman Wójcik ◽  
Joanna Małaczewska ◽  
Grzegorz Zwierzchowski ◽  
Jan Miciński ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Cell Mediated Immunity ◽  
Specific Cell ◽  
Newborn Calves ◽  
Escherichia Coli Bacteria ◽  
Leucine Metabolite

Abstract Background A healthy immune system plays a particularly important role in newborns, including in calves that are far more susceptible to infections (viral, bacterial and other) than adult individuals. Therefore, the present study aimed to evaluate the influence of HMB on the chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit) and oxidative burst (BURSTTEST® kit) of monocytes and granulocytes in the peripheral blood of calves by flow cytometry. Results An analysis of granulocyte and monocyte chemotactic activity and phagocytic activity revealed significantly higher levels of phagocytic activity in calves administered HMB than in the control group, expressed in terms of the percentage of phagocytising cells and mean fluorescence intensity (MFI). HMB also had a positive effect on the oxidative metabolism of monocytes and granulocytes stimulated with PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria, expressed as MFI values and the percentage of oxidative metabolism. Conclusion HMB stimulates non-specific cell-mediated immunity, which is a very important consideration in newborn calves that are exposed to adverse environmental factors in the first weeks of their life. The supplementation of animal diets with HMB for both preventive and therapeutic purposes can also reduce the use of antibiotics in animal production.

scholarly journals The Effects of β-Hydroxy-β-Methylbutyrate (HMB) on Chemotaxis, Phagocytosis, and Oxidative Burst of Peripheral Blood Granulocytes and Monocytes in Goats

Animals ◽  
2019 ◽  
Vol 9 (12) ◽  
pp. 1031 ◽  
Author(s):  
Roman Wójcik ◽  
Katarzyna Ząbek ◽  
Joanna Małaczewska ◽  
Stanisław Milewski ◽  
Edyta Kaczorek-Łukowska
Keyword(s):  
Fluorescence Intensity ◽  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Mean Fluorescence Intensity ◽  
Chemotactic Activity ◽  
Control Group ◽  
Phagocytic Cells ◽  
Escherichia Coli Bacteria ◽  
Experimental Group

The objective of this study was to determine the effect of β-hydroxy-β-methylbutyrate (HMB) on the chemotactic activity, phagocytic activity, and oxidative metabolism of peripheral blood granulocytes and monocytes in goats. Goat kids aged 30 ± 3 days were divided into two groups of 12 animals each: I—control, and II—experimental. Experimental group animals were fed a diet supplemented with HMB in the amount of 50 mg/Kg BW; whereas the diets of control goats were not supplemented. At the beginning of the experiment (day 0) and on experimental days 15, 30, and 60, blood was sampled from the jugular vein to determine and compare chemotactic activity (MIGRATEST® kit), phagocytic activity (PHAGOTEST® kit), and oxidative metabolism (BURSTTEST® kit) of peripheral blood granulocytes and monocytes by flow cytometry. The analyses of the chemotactic and phagocytic activity of granulocytes and monocytes revealed statistically higher levels of phagocytic activity in the experimental group than in the control group, as expressed by the percentage of phagocytic cells and mean fluorescence intensity. HMB also enhanced the oxidative metabolism of both granulocytes and monocytes, expressed by the rate of oxidative metabolism and mean fluorescence intensity after stimulation with Escherichia coli bacteria and PMA (4-phorbol-12-β-myristate-13-acetate).

scholarly journals The effect of Leiber Beta-S (1,3-1,6-β-D-glucan) on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in calves

2014 ◽  
Vol 83 (4) ◽  
pp. 347-354 ◽  
Author(s):  
Roman Wójcik
Keyword(s):  
Escherichia Coli ◽  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Mean Fluorescence Intensity ◽  
Jugular Vein ◽  
Dietary Supplementation ◽  
Control Group ◽  
E Coli ◽  
Experimental Group

The effect of the Leiber Beta-S (1,3-1,6-β-D-glucan) dietary supplement on the phagocytic activity (Phagotest) and oxidative metabolism (Phagoburst) of peripheral blood granulocytes and monocytes in calves was determined by flow cytometry. Fourteen animals were divided into two groups: a control group without dietary supplementation and an experimental group administered 50 mg/kg body weight/day of 1,3-1,6-β-D-glucan for 60 days. At the beginning of the experiment (day 0) and on days 15, 30 and 60, blood was sampled from the jugular vein to determine and compare immunological indicators. Leiber Beta-S significantly influenced (P ≤ 0.05, P ≤ 0.01, P ≤ 0.001) the percentages of phagocytic granulocytes (days 15 and 30) and monocytes (days 15, 30 and 60), and the percentage of bacteria engulfed by granulocytes (days 15 and 30) and monocytes (day 30). Leiber Beta-S increased the percentage of cells generating a respiratory burst in the population of granulocytes stimulated with fMLP (N-formyl-met-leu-phe) (day 15), PMA (4-phorbol-12-β-myristate-13-acetate) and Escherichia coli bacteria (days 15 and 30), and in the population of monocytes stimulated with fMLP (day 30), PMA (days 30 and 60) and E. coli (days 15, 30 and 60). The analyzed supplement increased mean fluorescence intensity in granulocytes stimulated with E. coli and fMLP (days 15 and 30) and PMA (days 30 and 60) as well as in monocytes stimulated with PMA, E. coli and fMLP (day 60). The findings of this study contribute to a better understanding of the effects of Leiber Beta-S on phagocytosis in calves, which have not been investigated to date.

The effect of β-hydroxy-β-methylbutyrate (HMB) on the proliferative response of blood lymphocytes and the phagocytic activity of blood monocytes and granulocytes in calves

2013 ◽  
Vol 16 (3) ◽  
pp. 567-569 ◽  
Author(s):  
R. Wójcik ◽  
J. Małaczewska ◽  
A.K. Siwicki ◽  
J. Miciński ◽  
G. Zwierzchowski
Keyword(s):  
Respiratory Burst ◽  
Phagocytic Activity ◽  
Proliferative Response ◽  
Jugular Vein ◽  
Control Group ◽  
Blood Monocytes ◽  
Respiratory Burst Activity ◽  
Killing Activity ◽  
Group I ◽  
Experimental Group

Abstract The objective of this study was to evaluate the effect of HMB on selected indicators of immunity in calves. The experiment was performed on 14 calves aged 30±2 days, divided into two equal groups of control (group I) and experimental (group II) animals. The feed administered to experimental group calves was supplemented with HMB at 40 mg/kg BW, whereas control calves were administered standard farm-made feed without supplementation. Blood was sampled from the jugular vein immediately before the experiment (day 0) and on experimental days 15, 30 and 60 to determine the following parameters of immunity: proliferative response of LPS- and ConA-stimulated lymphocytes (MTT), respiratory burst activity (RBA) and potential killing activity (PKA) of phagocytes. The results revealed a significant increase in RBA and MTT values in calves administered HMB in comparison with the control group throughout the experiment. In the group of animals receiving HMB, an increase in PKA values was noted only on day 30.

The effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs

2014 ◽  
Vol 17 (2) ◽  
pp. 281-291 ◽  
Author(s):  
R. Wójcik
Keyword(s):  
Fluorescence Intensity ◽  
Oxidative Metabolism ◽  
Peripheral Blood ◽  
Phagocytic Activity ◽  
Mean Fluorescence Intensity ◽  
Jugular Vein ◽  
Phagocytic Cells ◽  
Escherichia Coli Bacteria ◽  
Positive Effect ◽  
Experimental Group

AbstractThe objective of this study was to determine the effect of Biolex-MB40 on the phagocytic activity and oxidative metabolism of peripheral blood granulocytes and monocytes in lambs. The experimental material comprised 32 lambs aged 30 ± 3 days, divided into two equal groups: control and experimental. Experimental group animals were fed a diet supplemented with the Biolex-MB40 (Saccharomyces cerevisiae) in the amount of 3 g/kg of the concentrate. At the beginning of the experiment (day 0) and on experimental days 15, 30 and 60, blood was sampled from the jugular vein to determine and compare the phagocytic activity (PHAGOTEST) and oxidative metabolism (BURSTTEST) of peripheral blood granulocytes and monocytes by flow cytometry. Based on the results of an analysis of granulocyte and monocyte phagocytic activity, statistically higher levels of phagocytic activity were observed in the group of lambs administered Biolex-MB40 than in the control animals, expressed in terms of the percentage of phagocytic cells as well as mean fluorescence intensity. Biolex-MB40 also had a positive effect on the oxidative metabolism of both granulocytes and monocytes after stimulation with Escherichia coli bacteria and with PMA (4-phorbol-12-β-myristate-13-acetate), expressed in terms of the percentage of oxidative metabolism as well as mean fluorescence intensity

Influence of Tobacco on Physical Fitness Recovery of College Students After Sports

2021 ◽  
Vol 7 (5) ◽  
pp. 3445-3451
Author(s):  
Chen Yake
Keyword(s):  
College Students ◽  
Physical Fitness ◽  
Aerobic Exercise ◽  
Negative Impact ◽  
Control Group ◽  
Exercise Time ◽  
Activity Intensity ◽  
Group I ◽  
The Difference ◽  
Experimental Group

Objectives: In this paper, the effects of tobacco on aerobic exercise ability and physical fitness recovery of college students were studied. Methods: University group sports intervention form: traditional characteristic project (basketball) + Taiji soft ball (R&D intervention project). Exercise time: 3 times/week; Activity duration: 30min; Activity intensity: the heart rate is controlled at 120-140 beats/min. All the college students in the experimental group are students who have never smoked, and the college students in the control group are students who have smoked for more than two years. The other conditions are the same. Results: The exercise time and endurance of experimental groups I and II were significantly lower than those of the control group, and the cardiopulmonary function was significantly lower than that of the control group. The indexes of experimental group II changed significantly compared with experimental group I, and the difference was statistically significant. Conclusion: Cigarette smoke can significantly reduce the aerobic exercise ability and anti fatigue ability. The longer the smoking time, the more serious the adverse effects. Therefore, tobacco smoke and nicotine will damage college students’ aerobic exercise ability and have a negative impact on the recovery of physical fitness after exercise.

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