Biological effect of Egyptian Propolis on BHK cell line and on dental pulp tissue (An In vitro And In vivo study)

2018 ◽  
Vol 64 (3) ◽  
pp. 2161-2170
Author(s):  
Marwa Moussa ◽  
Marwa Temirek
Keyword(s):  
Cell Line ◽  
Dental Pulp ◽  
Biological Effect ◽  
In Vivo Study ◽  
Pulp Tissue ◽  
Dental Pulp Tissue

scholarly journals Human Dental Pulp Tissue during Orthodontic Tooth Movement: An Immunofluorescence Study

2020 ◽  
Vol 5 (3) ◽  
pp. 65 ◽  
Author(s):  
Giovanna Vermiglio ◽  
Antonio Centofanti ◽  
Giovanni Matarese ◽  
Angela Militi ◽  
Marco Matarese ◽  
...  
Keyword(s):  
Protein Expression ◽  
Dental Pulp ◽  
Tooth Movement ◽  
Orthodontic Tooth Movement ◽  
Control Group ◽  
Pulp Tissue ◽  
Force Application ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp

The orthodontic tooth movement is the last step of several biological processes that take place after the application of external forces. During this process, dental pulp tissue is subjected to structural and protein expression modifications in order to maintain their integrity and functional morphology. The purpose of the present work was to perform an in vivo study, evaluating protein expression modifications in the human dental pulp of patients that have undergone orthodontic tooth movement due to pre-calibrated light force application for 30 days. Dental pulp samples were extracted from molars and premolars of the control group and after 7 and 30 days of treatment; the samples were then processed for immunofluorescence reactions using antibodies against fibronectin, collagen I and vascular endothelial growth factor (VEGF). Our results show that, after 7 days of treatment, all tested proteins change their pattern expression and will reset after 30 days. These data demonstrate that the dental pulp does not involve any irreversible iatrogenic alterations, supporting the efficacy and safety of using pre-calibrated force application to induce orthodontic tooth movement in clinical practice.

scholarly journals Regenerative potential of human dental pulp stem cells in the treatment of stress urinary incontinence: In vitro and in vivo study

2019 ◽  
Vol 52 (6) ◽  
Author(s):  
Alessio Zordani ◽  
Alessandra Pisciotta ◽  
Laura Bertoni ◽  
Giulia Bertani ◽  
Antonio Vallarola ◽  
...  
Keyword(s):  
Stem Cells ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
In Vivo Study ◽  
Human Dental Pulp

scholarly journals Laminin-Modified Dental Pulp Extracellular Matrix for Dental Pulp Regeneration

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiahui Fu ◽  
Jianfeng Chen ◽  
Wenjun Li ◽  
Xiaomin Yang ◽  
Jingyan Yang ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Dental Pulp ◽  
Matrix Protein ◽  
Immunofluorescent Staining ◽  
Pulp Tissue ◽  
Pulp Regeneration ◽  
Odontogenic Differentiation ◽  
Dental Pulp Tissue ◽  
Dentin Matrix

Native dental pulp extracellular matrix (DPEM) has proven to be an effective biomaterial for dental pulp regeneration. However, as a significant extracellular matrix glycoprotein, partial laminins were lost during the decellularization process, which were essential for odontoblast differentiation. Thereby, this study investigated the feasibility of LN supplementation to improve the surface of DPEM for odontoblast layer regeneration. The influences of laminin on cell adhesion and odontogenic differentiation were evaluated in vitro. Then, we fabricated laminin-modified DPEM based on the physical coating strategy and observed the location and persistency of laminin coating by immunofluorescent staining. Finally, laminin-modified DPEM combined with treated dentin matrix (TDM) was transplanted in orthotopic jaw bone of beagles (n = 3) to assess the effect of LNs on dental pulp tissue regeneration. The in vitro results showed that laminins could improve the adhesion of dental pulp stem cells (DPSCs) and promoted DPSCs toward odontogenic differentiation. Continuous odontoblastic layer-like structure was observed in laminin-modified DPEM group, expressing the markers for odontoblastogenesis, dentine matrix protein-1 (DMP-1) and dentin sialophosphoprotein (DSPP). Overall, these studies demonstrate that the supplementation of laminins to DPEM contributes to the odontogenic differentiation of cells and to the formation of odontoblast layer in dental pulp regeneration.

3D Printing of Stem Cell Responsive Ionically-Crosslinked Polyethylene Glycol Diacrylate/ Alginate Composite Hydrogels Loaded with Basic Fibroblast Growth Factor for Dental Pulp Tissue Engineering: A Preclinical Evaluation in Animal Model

2019 ◽  
Vol 9 (12) ◽  
pp. 1635-1643 ◽  
Author(s):  
Song Feng ◽  
Jingming Liu ◽  
Murugan Ramalingam
Keyword(s):  
Dental Pulp ◽  
Fibroblast Growth ◽  
Mass Ratio ◽  
Pulp Tissue ◽  
Polyethylene Glycol Diacrylate ◽  
Glycol Diacrylate ◽  
Dental Pulp Tissue ◽  
3D Printed ◽  
Concentration Ratios

The main aim of this study was to develop a 3D printed stem cell-responsive polyethylene glycol diacrylate (PEGDA) and sodium alginate (SA) composite hydrogel system (PEGDA/SA) loaded with basic fibroblast growth factor (bFGF) suitable for dental pulp tissue engineering. The PEGDA/SA loaded with bFGF (PEGDA/SA-bFGF) at different concentration ratios were 3D printed via stereolithography (SLA) under optimal processing conditions, followed by ionic crosslinking. The morphological and porous structure of the 3D printed PEGDA/SA-bFGF constructs and release profile of the bFGF were analyzed. The cellular compatibility of the hydrogel constructs was evaluated using human dental pulp stem cells (hDPSCs)in vitro. Subsequently, biocompatibility and tissue regenerative potential of the hydrogel constructs were evaluated in immunodeficient mice model. The in vitro cell culture results of PEGDA/SA-bFGF constructs exhibited high cell compatibility and supported cell proliferation, irrespective of their concentration ratios. Thein vivo animal experiment results showed that the hDPSCs-laden PEGDA/SA (PEGDA/SA-hDPSCs) group failed to form a good pulp structure even at various concentration ratios. When the mass ratio of PEGDA/SA was 25:1, hDPSCs-laden PEGDA/SA-bFGF (PEGDA/SA-bFGF-hDPSCs) group also failed to form dental pulp-like tissue but a partial loose connective tissue formation was observed. However, when the mass ratio of PEGDA/SA reduces to 20:1 ~ 15:1, the PEGDA/SA-bFGF-hDPSCs group tends to form a well-organized pulp structure post 4 weeks implantation, thus highlighting the potential of this particular hydrogel scaffolding system for dental pulp tissue regenerative applications.

scholarly journals Anti-Tumor Activity of Eurycoma longifolia Root Extracts against K-562 Cell Line: In Vitro and In Vivo Study

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e83818 ◽  
Author(s):  
Omar Saeed Ali Al-Salahi ◽  
Dan Ji ◽  
Amin Malik Shah Abdul Majid ◽  
Chan Kit-Lam ◽  
Wan Zaidah Abdullah ◽  
...  
Keyword(s):  
Cell Line ◽  
In Vivo Study ◽  
Root Extracts ◽  
Eurycoma Longifolia ◽  
Anti Tumor Activity

The in vitro and in vivo Influence of 4-META/MMA-TBB Resin Components on Dental Pulp Tissues

2001 ◽  
Vol 15 (1) ◽  
pp. 101-104 ◽  
Author(s):  
T. Inoue ◽  
S. Miyakoshi ◽  
M. Shimono
Keyword(s):  
Dental Pulp ◽  
Superficial Layer ◽  
Rabbit Kidney ◽  
Hybrid Layer ◽  
Pulp Tissue ◽  
Adhesive Resin ◽  
Osteogenic Activity ◽  
In Vivo Effects

The purpose of this study was to qualitate the penetration of the major components of 4-META/MMA-TBB adhesive resin (4-META resin) and to characterize their influence on the in vitro and in vivo wound healing of dental pulp tissues. Fresh 4-META resin was applied to rabbit mesentery; its components penetrated the mesentery to form three of layers, depending on the amounts of monomer components in the tissue. The superficial layer was a soft-tissue hybrid layer (STHL), the intermediate layer contained small particles of polymerized 4-META resin, while the deepest layer contained unpolymerized monomer components including MMA and butanol, which were detected by gas chromatography (GC). To characterize the in vivo effects of the deepest layer, we immersed the pulp tissue in MMA or in 5% 4-META/MMA and autotransplanted it to placement beneath a rabbit kidney capsule. The MMA-immersed pulp was positive for osteocalcin and presented osteodentin formation at 7 days, as did the untreated control pulp tissue. In contrast, the 5% 4-META/MMA-immersed pulp collapsed into the cell-deficient fibrous connective tissue, with slight calcification by 7 days and less osteodentin formation at 14 days. Analysis of these data suggests that MMA does not inhibit osteogenic activity of pulp tissue, while 5% 4-META/MMA does inhibit osteogenic activity to some extent.

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