scholarly journals A novel high performance metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

2020 ◽  
Author(s):  
Shohreh Ariaeenejad ◽  
Atefeh Sheykhabdolahzadeh ◽  
Morteza Maleki ◽  
Kaveh Kavousi ◽  
Mehdi Foroozandeh Shahraki ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Specific Activity ◽  
Agricultural Waste ◽  
Extreme Temperature ◽  
In Silico Analysis ◽  
Cellulose Content ◽  
Biomass Hydrolysis ◽  
Harsh Condition

Abstract Background: Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results: The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. Using a multi-step in-silico analysis, primary candidates with desired properties were found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes' kinetic parameters, including V max , Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85°C and was able to retain more than 70% of its activity after 150 hours of storage at 85°C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl, MgCl 2 , CaCl 2 , and MnCl 2 . Our results showed that treatment with MnCl 2 could enhance the enzyme’s activity by 89%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 hours in the harsh condition ( T= 85°C, pH = 8.5). Conclusion: Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.

scholarly journals A novel high performance in-silico screened metagenome-derived alkali-thermostable endo-β-1,4-glucanase for lignocellulosic biomass hydrolysis in the harsh conditions

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Shohreh Ariaeenejad ◽  
Atefeh Sheykh Abdollahzadeh Mamaghani ◽  
Morteza Maleki ◽  
Kaveh Kavousi ◽  
Mehdi Foroozandeh Shahraki ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
In Silico ◽  
Specific Activity ◽  
Agricultural Waste ◽  
Extreme Temperature ◽  
Cellulose Content ◽  
Biomass Hydrolysis ◽  
Harsh Condition

Abstract Background Lignocellulosic biomass, is a great resource for the production of bio-energy and bio-based material since it is largely abundant, inexpensive and renewable. The requirement of new energy sources has led to a wide search for novel effective enzymes to improve the exploitation of lignocellulose, among which the importance of thermostable and halotolerant cellulase enzymes with high pH performance is significant. Results The primary aim of this study was to discover a novel alkali-thermostable endo-β-1,4-glucanase from the sheep rumen metagenome. At first, the multi-step in-silico screening approach was utilized to find primary candidate enzymes with superior properties. Among the computationally selected candidates, PersiCel4 was found and subjected to cloning, expression, and purification followed by functional and structural characterization. The enzymes’ kinetic parameters, including Vmax, Km, and specific activity, were calculated. The PersiCel4 demonstrated its optimum activity at pH 8.5 and a temperature of 85 °C and was able to retain more than 70% of its activity after 150 h of storage at 85 °C. Furthermore, this enzyme was able to maintain its catalytic activity in the presence of different concentrations of NaCl and several metal ions contains Mg2+, Mn2+, Cu2+, Fe2+ and Ca2+. Our results showed that treatment with MnCl2 could enhance the enzyme’s activity by 78%. PersiCel4 was ultimately used for enzymatic hydrolysis of autoclave pretreated rice straw, the most abundant agricultural waste with rich cellulose content. In autoclave treated rice straw, enzymatic hydrolysis with the PersiCel4 increased the release of reducing sugar up to 260% after 72 h in the harsh condition (T = 85 °C, pH = 8.5). Conclusion Considering the urgent demand for stable cellulases that are operational on extreme temperature and pH conditions and due to several proposed distinctive characteristics of PersiCel4, it can be used in the harsh condition for bioconversion of lignocellulosic biomass.

scholarly journals Peran Mikroba Dekomposer Selulolitik dari Sarang Rayap dalam Menurunkan Kandungan Selulosa Limbah Pertanian Berselulosa Tinggi

SoilREns ◽  
2020 ◽  
Vol 17 (2) ◽  
Author(s):  
Mieke Rochimi Setiawati ◽  
Nizar Ulfah ◽  
Pujawati Suryatmana ◽  
Reginawanti Hindersah
Keyword(s):  
Rice Straw ◽  
Oil Palm ◽  
Cellulose Degradation ◽  
Agricultural Waste ◽  
Block Design ◽  
Organic Fertilizer ◽  
Added Value ◽  
Cellulose Content ◽  
Cellulosic Material ◽  
Empty Fruit Bunches

Agricultural waste is rich in cellulosic material that is difficult to degrade but can be decomposed by cellulase enzymes. Cellulosic material is an organic component abundantly in nature in the form of cellulose and hemicellulose polymer. Utilization of microbes in the process of waste bioconversion can be done to get added value from the waste material into other products such as organic fertilizer. Cellulase-producing microbes can be isolated from various sources, one of which is from termite nests. The use of microbes from termite nests as decomposers for composting high-yielding agricultural waste was carried out in this study. Cellulolytic microbial isolates (combination of bacteria and fungi and without decomposers) were applied to agricultural wastes high in cellulose content: rice straw, bagasse, and oil palm empty fruit bunches. Randomized Block Design was applied and each treatment was and repeated 3 times. The composting process was carried out for 1 month indoors. The results showed that cellulolytic microbial consortium obtained from termite nests reduced the compost cellulose content of bagasse agricultural waste in one month. Whereas rice straw and oil palm empty fruit bunches showed a tendency to decrease cellulose content because cellulose degradation by decomposer microbes still continues after one month of incubation.

scholarly journals Improvement of potassium permanganate pretreatment by enzymatic saccharification of rice straw for production of biofuels

2021 ◽  
Vol 302 ◽  
pp. 02013
Author(s):  
Parita Mutrakulcharoen ◽  
Peerapong Pornwongthong ◽  
S.T Anne Sahithi ◽  
Theerawut Phusantisampan ◽  
Atthasit Tawai ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Rice Straw ◽  
Potassium Permanganate ◽  
Agricultural Waste ◽  
Complex Structure ◽  
Enzymatic Saccharification ◽  
Value Added ◽  
Reducing Sugar ◽  
Optimum Conditions ◽  
Pm2.5 And Pm10

Commonly, the agricultural waste, i.e. lignocellulosic biomass is disposed through combustion causing air pollution with production of PM2.5 and PM10 particles. However, it has been found that these biomasses can be used as source for the production of biofuels and other valuable biochemicals. Though deconstruction of lignocellulosic biomass is challenging due to its complex structure. In this study, rice straw (RS) was pretreated using potassium permanganate (KMnO4) to enhance the enzymatic saccharification efficiency. The study was carried out by varying the operational factors in pretreatment, including temperature (30-90°C), time (30-360 min) and concentration of KMnO4 (0.5-3.0, % w/v), respectively, based on Box-Behnken design (BBD). Through multi-regression analysis of the experimental data obtained after pretreatment, the optimum conditions were determined. The optimum conditions for temperature, time and potassium permanganate concentration were 48.09°C, 360 min, and 1.36% w/v, respectively. The saccharifications of pretreatment and untreated rice straw were carried out using Cellic Ctec2. The reducing sugar was determined by using DNS method and the yields of the untreated and pretreated RS were 32.38 and 49.011 mg/mL, respectively. The results showed that the sugar for pretreated RS were 1.51 fold times higher compared to untreated RS. Therefore, this work illustrates the pretreatment efficiency for KMnO4 to enhance the reducing sugar yield during saccharification, which can be used for biofuel and value-added product productions.

KINETIC AND ENZYME RECYCLING STUDIES OF IMMOBILIZED b-GLUCOSIDASE FOR LIGNOCELLULOSIC BIOMASS HYDROLYSIS

2018 ◽  
Vol 17 (6) ◽  
pp. 1385-1398 ◽  
Author(s):  
Deepak K. Tuli ◽  
Ruchi Agrawal ◽  
Alok Satlewal ◽  
Anshu S. Mathur ◽  
Ravi P. Gupta ◽  
...  
Keyword(s):  
Lignocellulosic Biomass ◽  
Biomass Hydrolysis ◽  
Enzyme Recycling

Recycling cellulases by pH-triggered adsorption-desorption during the enzymatic hydrolysis of lignocellulosic biomass

2014 ◽  
Vol 98 (12) ◽  
pp. 5765-5774 ◽  
Author(s):  
Yaping Shang ◽  
Rongxin Su ◽  
Renliang Huang ◽  
Yang Yang ◽  
Wei Qi ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Lignocellulosic Biomass ◽  
Adsorption Desorption ◽  
Hydrolysis Of

scholarly journals Generation of highly amenable cellulose-Iβ via selective delignification of rice straw using a reusable cyclic ether-assisted deep eutectic solvent system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chiranjeevi Thulluri ◽  
Ravi Balasubramaniam ◽  
Harshad Ravindra Velankar
Keyword(s):  
Enzymatic Hydrolysis ◽  
Rice Straw ◽  
Deep Eutectic Solvent ◽  
Solvent System ◽  
Cyclic Ether ◽  
Cellulolytic Enzymes ◽  
Ammonium Bromide ◽  
Selective Delignification ◽  
Cellulose Iβ ◽  
Hydrolysis Of

AbstractCellulolytic enzymes can readily access the cellulosic component of lignocellulosic biomass after the removal of lignin during biomass pretreatment. The enzymatic hydrolysis of cellulose is necessary for generating monomeric sugars, which are then fermented into ethanol. In our study, a combination of a deep eutectic (DE) mixture (of 2-aminoethanol and tetra-n-butyl ammonium bromide) and a cyclic ether (tetrahydrofuran) was used for selective delignification of rice straw (RS) under mild conditions (100 °C). Pretreatment with DE-THF solvent system caused ~ 46% delignification whereas cellulose (~ 91%) and hemicellulose (~ 67%) recoveries remained higher. The new solvent system could be reused upto 10 subsequent cycles with the same effectivity. Interestingly, the DE-THF pretreated cellulose showed remarkable enzymatic hydrolysability, despite an increase in its crystallinity to 72.3%. Contrary to conventional pretreatments, we report for the first time that the enzymatic hydrolysis of pretreated cellulose is enhanced by the removal of lignin during DE-THF pretreatment, notwithstanding an increase in its crystallinity. The current study paves way for the development of newer strategies for biomass depolymerization with DES based solvents.

Utilization of rice straw and tea composts for strawberry and tomato production

2020 ◽  
Vol 30 (4) ◽  
pp. 335-343
Author(s):  
R Ashrafi ◽  
RM Saiem ◽  
M Kamruzzaman ◽  
MSAA Mamun ◽  
HA Begum
Keyword(s):  
Rice Straw ◽  
Agricultural Waste ◽  
Total Sugar ◽  
Fruit Yield ◽  
Compost Tea ◽  
Tomato Production ◽  
Horticultural Crops ◽  
Beneficial Effects ◽  
Combined Application ◽  
Yield And Quality

The use of agricultural waste is of great interest to sustainable agriculture. An investigation was carried out to evaluate the effects of compost and compost tea made from agricultural waste rice straw on the yield and quality of two horticultural crops strawberry and tomato. In strawberry experiment, six treatments were considered which wereT1: 100% soil (as a control), T2: 80% soil + 20% compost, T3: 60% soil + 40% compost, T4: 40% soil + 60% compost, T5: 20% soil + 80% compost & T6: 100% compost. Results revealed that number of fruit, fruit yield and total sugar (%)was increased with the increasing level of compost up to 80% after that decreased at 100% compost. The treatment T5: 20% soil + 80% compost gave the best results among the treatments to grow strawberry with good yield (185.3 g/plant) and sweetness (total sugar 5.19%). On the other hand, six treatments i.e. T1: Control, T2: Compost (5 t/ha), T3: Compost tea (50% concentration), T4: Compost (5 t/ha) + Compost tea (50% concentration), T5: Compost tea (100% concentration) and T6: Compost (5t/ha) + Compost tea (100% concentration) were considered in tomato experiment. As a result, it was observed that combined application of compost (5 t/ha) along with compost tea (100% conc.) (T6) gave the highest yield of tomato than not only control (T1) but also single application of compost or compost tea (T2, T3, T4&T5). Level of compost tea concentration also showed significant effect on fruit yield of tomato. Comparing between treatment T4 and T6, fruit yield was found higher (1027.67 g/plant) in treatment T6: Compost (5t/ha) + Compost tea (100% conc.) than 961.3 g/plant in T4: Compost (5 t/ha) + Compost tea (50% conc.). So, it could be summarized that use of rice straw as compost and compost tea affects positively both in two experiments. The results of this study confirm the beneficial effects of compost to increase the yield and sweetness of strawberry and combination of compost and compost tea to increase the yield of tomato. Progressive Agriculture 30 (4): 335-343, 2019

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