scholarly journals Epidemiology and Clinical Features of Viral Anterior Uveitis in Southern Taiwan—Diagnosis with Polymerase Chain Reaction

2019 ◽  
Author(s):  
Yu-Ting Hsiao ◽  
Ming-Tse Kuo ◽  
Wei-Yu Chiang ◽  
Tsai-Ling Chao ◽  
Hsi-Kung Kuo
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Features ◽  
Anterior Uveitis ◽  
Case Series ◽  
Pcr Analysis ◽  
Chang Gung Memorial Hospital ◽  
Chain Reaction ◽  
Case Series Study ◽  
Southern Taiwan ◽  
Polymerase Chain

Abstract Background To report the epidemiology and clinical features of viral anterior uveitis in patients in southern Taiwan. Methods A retrospective, case series study. HLA-B27 negative anterior uveitis patients with increased intraocular pressure or corneal edema seen at Kaohsiung Chang Gung Memorial Hospital from January 1, 2007 to January 31, 2018 had their aqueous sent for polymerase chain reaction analysis. Their records were reviewed for demographic data, ocular findings, and laboratory results. Results In the aqueous samples obtained from 102 eligible eyes, 42 eyes were herpesviridae-positive, which included 9 with herpes simplex virus (8.8%), 5 with varicella-zoster virus (4.9%), 27 with cytomegalovirus (26.5%), and 1 with Epstein-Barr virus (1%). Herpesviridae-positive patients were more likely to be male, and have glaucoma. Glaucoma and pseudophakic eyes were significantly associated with CMV-positive eyes. Conclusion PCR analysis of the anterior chamber fluid is important for the confirmation of the diagnosis of viral anterior uveitis. Cytomegalovirus anterior uveitis is not uncommon in patients in southern Taiwan, and it may follow an uneventful cataract extraction in immunocompetent patients.

scholarly journals Epidemiology and Clinical Features of Viral Anterior Uveitis in Southern Taiwan—Diagnosis with Polymerase Chain Reaction

2019 ◽  
Author(s):  
Yu-Ting Hsiao ◽  
Ming-Tse Kuo ◽  
Wei-Yu Chiang ◽  
Tsai-Ling Chao ◽  
Hsi-Kung Kuo
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Features ◽  
Anterior Uveitis ◽  
Case Series ◽  
Pcr Analysis ◽  
Chang Gung Memorial Hospital ◽  
Chain Reaction ◽  
Case Series Study ◽  
Southern Taiwan ◽  
Polymerase Chain

Abstract Background To report the epidemiology and clinical features of viral anterior uveitis in patients in southern Taiwan. Methods A retrospective, case series study. HLA-B27 negative anterior uveitis patients with increased intraocular pressure or corneal edema seen at Kaohsiung Chang Gung Memorial Hospital from January 1, 2007 to January 31, 2018 had their aqueous sent for polymerase chain reaction analysis. Their records were reviewed for demographic data, ocular findings, and laboratory results. Results In the aqueous samples obtained from 102 eligible eyes, 42 eyes were herpesviridae-positive, which included 9 with herpes simplex virus (8.8%), 5 with varicella-zoster virus (4.9%), 27 with cytomegalovirus (26.5%), and 1 with Epstein-Barr virus (1%). Herpesviridae-positive patients were more likely to be male, and have glaucoma. Glaucoma and pseudophakic eyes were significantly associated with CMV-positive eyes. Conclusion PCR analysis of the anterior chamber fluid is important for the confirmation of the diagnosis of viral anterior uveitis. Cytomegalovirus anterior uveitis is not uncommon in patients in southern Taiwan, and it may follow an uneventful cataract extraction in immunocompetent patients.

scholarly journals Epidemiology and Clinical Features of Viral Anterior Uveitis in Southern Taiwan—Diagnosis with Polymerase Chain Reaction

2019 ◽  
Author(s):  
Yu-Ting Hsiao ◽  
Ming-Tse Kuo ◽  
Wei-Yu Chiang ◽  
Tsai-Ling Chao ◽  
Hsi-Kung Kuo
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Features ◽  
Anterior Uveitis ◽  
Case Series ◽  
Pcr Analysis ◽  
Chang Gung Memorial Hospital ◽  
Chain Reaction ◽  
Case Series Study ◽  
Southern Taiwan ◽  
Polymerase Chain

Abstract Background To report the epidemiology and clinical features of viral anterior uveitis in patients in southern Taiwan. Methods A retrospective, case series study. HLA-B27 negative anterior uveitis patients with increased intraocular pressure or corneal edema seen at Kaohsiung Chang Gung Memorial Hospital from January 1, 2007 to January 31, 2018 had their aqueous sent for polymerase chain reaction analysis. Their records were reviewed for demographic data, ocular findings, and laboratory results. Results In the aqueous samples obtained from 102 eligible eyes, 42 eyes were herpesviridae-positive, which included 9 with herpes simplex virus (8.8%), 5 with varicella-zoster virus (4.9%), 27 with cytomegalovirus (26.5%), and 1 with Epstein-Barr virus (1%). Herpesviridae-positive patients were more likely to be male, and have glaucoma. Glaucoma and pseudophakic eyes were significantly associated with CMV-positive eyes. Conclusion PCR analysis of the anterior chamber fluid is important for the confirmation of the diagnosis of viral anterior uveitis. Cytomegalovirus anterior uveitis is not uncommon in patients in southern Taiwan, and it may follow an uneventful cataract extraction in immunocompetent patients.

scholarly journals The Value of Routine Polymerase Chain Reaction Analysis of Intraocular Fluid Specimens in the Diagnosis of Infectious Posterior Uveitis

2013 ◽  
Vol 2013 ◽  
pp. 1-8 ◽  
Author(s):  
Marius A. Scheepers ◽  
Karin A. Lecuona ◽  
Graeme Rogers ◽  
Catey Bunce ◽  
Craig Corcoran ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Diagnosis ◽  
Tertiary Care ◽  
Case Series ◽  
Posterior Uveitis ◽  
Pcr Analysis ◽  
Care Hospital ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Intraocular Fluid

Objective. To assess the value of routine polymerase chain reaction (PCR) analysis on intraocular fluid from patients presenting with a first episode of suspected active infectious posterior uveitis in a population with a high prevalence of human immunodeficiency virus infection.Design. Retrospective, interventional case series.Participants. 159 consecutive patients presenting at a tertiary care hospital over a five-year period.Methods. PCR analysis was performed for cytomegalovirus, varicella zoster virus, herpes simplex virus types 1 and 2,Toxoplasma gondii, andMycobacterium tuberculosis.Results. PCR analysis confirmed the initial clinical diagnosis in 55 patients (35%) and altered the initial clinical diagnosis in 36 patients (23%). The clinical diagnosis prior to PCR testing was nonspecific (uncertain) in 51 patients (32%), with PCR providing a definitive final diagnosis in 20 of these patients (39%); necrotizing herpetic retinopathy and ocular toxoplasmosis were particularly difficult to diagnose correctly without the use of PCR analysis.Conclusion. The clinical phenotype alone was unreliable in diagnosing the underlying infectious cause in a quarter of patients in this study. Since the outcome of incorrectly treated infective uveitis can be blinding, PCR analysis of ocular fluids is recommended early in the disease even in resource poor settings.

6166 Findings In Detection Of Herpesviridae By Real-time Polymerase Chain Reaction And Intraocular Antibody Production In A Case-series Of Anterior Uveitis

SciVee ◽  
2012 ◽  
Author(s):  
Marie-Helene Errera ◽  
Pablo Goldschmidt ◽  
Laurence Batellier ◽  
Sandrine Degorge ◽  
Emmanuel Héron ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Real Time ◽  
Antibody Production ◽  
Anterior Uveitis ◽  
Case Series ◽  
Chain Reaction ◽  
Polymerase Chain

Findings in Detection ofHerpesviridaeby Polymerase Chain Reaction and Intraocular Antibody Production in a Case Series of Anterior Uveitis

2013 ◽  
Vol 21 (1) ◽  
pp. 61-68 ◽  
Author(s):  
Marie-Hélène Errera ◽  
Pablo Goldschmidt ◽  
Laurence Batellier ◽  
Sandrine Degorge ◽  
Emmanuel Héron ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Antibody Production ◽  
Anterior Uveitis ◽  
Case Series ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals A Case Series of Patients Suspected to Have Covid-19 Based on Clinical Features, Laboratory Markers and Imaging Studies but Who Were RT-PCR Negative

2021 ◽  
Vol 8 (15) ◽  
pp. 982-988
Author(s):  
Dilip Pandurang Patil ◽  
Nikhil Dilip Patil
Keyword(s):  
Computed Tomography ◽  
Polymerase Chain Reaction ◽  
Clinical Features ◽  
Case Series ◽  
Rt Pcr ◽  
Imaging Studies ◽  
Chain Reaction ◽  
High Resolution Computed Tomography ◽  
Laboratory Markers ◽  
Polymerase Chain

BACKGROUND Real-time reverse transcription polymerase chain reaction (rRTPCR) test is the gold standard assay to diagnose Covid-19 in-spite of few concerns. The disease is suspected in patients presenting with clinical features suggestive of Covid-19 supported by laboratory markers and imaging studies. We describe a case series of ten patients suspected to have SARS CoV-2 infection but RTPCR negative. METHODS We carried out a retrospective observational study of patients who presented with clinical features suggestive of Covid-19 but were RTPCR negative. As dedicated Covid hospitals, RTPCR negative patients were not admitted because of overload. We admitted such patients in isolation ward in non Covid hospitals, evaluated them further with laboratory biomarkers, and imaging studies. All patients were categorised in disease severity and were managed according to guidelines of Ministry of Health and Family Welfare (MoHFW) India. RESULTS Our case series included ten patients with a mean age of 55.1 years and the male to female ratio was 1.5:1. All patients (100 %) were suspected to have SARS CoV2 infection. Commonest symptom was fever in all (100 %) and least common was diarrhoea in 30 % patients. Mean SpO2 was 92.5 % on pulse oximeter (range 91 to 94 %). The laboratory findings showed (median): white blood cell count 9400 / cumm, N / L ratio 3.78, C-reactive protein (CRP) 46.21 mg / L, D-dimer 465 ng / mL, lactate dehydrogenase 499.59 U / L, serum ferritin 160.55 ng / mL, interleukin 6 31.6 pg / mL. Chest radiograph showed patchy non homogenous opacities in lungs in 6 (60 %) out of 10 patients, high resolution computed tomography (HRCT) chest revealed peripheral ground glass opacities (GGOs) in 10 (100 %) patients, Coronavirus Disease 2019 Reporting and Data System (CORADS) score was 5 in 8 (80 %) patients and less than 5 in 2 (20 %) patients. CONCLUSIONS In current pandemic, patients presenting with clinical features suggestive of Covid19, but RT-PCR negative should be suspected to have SARS CoV-2 infection, further evaluation with laboratory markers and imaging study help in diagnosis. Managing and monitoring according to MoHFW guidelines show good clinical recovery. KEYWORDS Corona Virus Disease (Covid-19), Real-Time Reverse Transcription Polymerase Chain Reaction (RT-PCR), High Resolution Computed Tomography (HRCT), Chest X-Ray (CXR)

Sensitivity of polymerase chain reaction (PCR)-southern hybridization and conventional PCR analysis for Halal authentication of gelatin capsules

LWT ◽  
2015 ◽  
Vol 63 (1) ◽  
pp. 714-719 ◽  
Author(s):  
Sahilah Abd Mutalib ◽  
Nursheila Mustafa Muin ◽  
Aminah Abdullah ◽  
Osman Hassan ◽  
Wan Aida Wan Mustapha ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Southern Hybridization ◽  
Pcr Analysis ◽  
Conventional Pcr ◽  
Chain Reaction ◽  
Gelatin Capsules ◽  
Halal Authentication ◽  
Polymerase Chain

Development of Three-Stage Bioaerosol Sampler for Size-Selective Sampling

2022 ◽  
Author(s):  
Jun-Hyung Lim ◽  
Sang Hwan Nam ◽  
Jongwoo Kim ◽  
Nam Hoon Kim ◽  
Gun-Soo Park ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Size Range ◽  
Collection Efficiency ◽  
Commercial Product ◽  
Pcr Analysis ◽  
Cyclone Separator ◽  
Chain Reaction ◽  
Selective Sampling ◽  
Sampling Flow Rate ◽  
Polymerase Chain

Abstract In this study, a three-stage bioaerosol sampler with a sampling flow rate of 170 L/min was designed and fabricated for sampling the bioaerosols released during human breathing and coughing, and its performance was evaluated. The sampler was constructed using a cyclone separator with a cutoff size of 2.5 µm as a preseparator, a multi-nozzle virtual impactor with a cutoff size of 0.34 µm as an aerosol concentrator, and a BioSampler, which is a commercial product, for collecting bioaerosols in a collection fluid. The collection efficiency of the sampler was evaluated through simulations and experiments. Only particles with sizes of 0.1-4 µm were selectively collected in the collection fluid. Bacteriophage bioaerosols were sampled using the developed sampler and ACD-200 Bobcat sampler, which is a commercial product. The amounts of collected bacteriophages were compared using the polymerase chain reaction (PCR) technique. The sampling performance of the developed sampler was similar to that of the ACD-200 Bobcat sampler. Moreover, the developed sampler showed its ability to sample bioaerosols of a specific size-range and collect them directly in a collection fluid for the PCR analysis. Therefore, the developed sampler is expected to be useful for indoor environmental monitoring by effectively sampling the bioaerosols released indoors during human breathing and coughing.

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