scholarly journals Identification Candidate Diagnostic Biomarkers Between Minimal Change Disease and Focal Segmental Glomerulosclerosis by Bioinformatics Analysis

2020 ◽  
Author(s):  
Xue Jiang ◽  
Laite Chen ◽  
Yuanyuan Du ◽  
Yayu Li
Keyword(s):  
Differential Diagnosis ◽  
Differential Expression ◽  
Focal Segmental Glomerulosclerosis ◽  
Minimal Change Disease ◽  
Bioinformatics Analysis ◽  
Minimal Change ◽  
Hub Genes ◽  
U6 Snrna ◽  
Segmental Glomerulosclerosis ◽  
Healthy Control

Abstract Background: Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are common causes of nephrotic syndrome which have similar clinical as well as histologic magnification and hard to differentiate. This study aimed to identify novel biomarkers to distinguish FSGS and MCD through bioinformatics analysis and elucidate the possible molecular mechanism.Methods: Based on the microarray datasets GSE104948 and GSE108113 downloaded from the Gene Expression Omnibus database, the differentially expressed genes (DEGs) between FSGS vs healthy control , MCD vs healthy control were identified, and further defined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Hub genes were checked by protein-protein interaction networks.Results: A total of and 358 and 368 genes were identified in FSGS and MCD compared with healthy controls, among them, there were156 overlapping DEGs. GO analysis showed the DEGs in these two diseases were simultaneously enriched in mRNA splicing, RNA polymerase II transcription, mRNA export, insulin stimulus, integrin-mediated signaling pathway, viral process and phagocytosis. Module analysis showed that genes in the top 1 significant module of the PPI network were mainly associated with Spliceosome among FSGS and MCD. The top 10 hub genes analysis discovered that Most of hub genes were same between two disease, while among these genes, CD2 cytoplasmic tail binding protein 2 (CD2BP2), U6 snRNA-associated Sm-like protein (LSM8) and Small nuclear ribonucleoprotein polypeptides B (SNRPB) only differential expression in FSGS and Splicing factor 3A, subunit 3 (SF3A3) only differential expression in MCD, which may be used for differential diagnosis of these two diseases in the future.Conclusions: We identified key genes and mainly pathway associated with FSGS and MCD. Our results provide a set of potential genes used for differential diagnosis of these two diseases.

scholarly journals Identification Candidate Diagnostic Biomarkers Between Minimal Change Disease and Focal Segmental Glomerulosclerosis by Bioinformatics Analysis

2021 ◽  
Author(s):  
Yayu Li ◽  
Yuanyuan Du ◽  
Xue Jiang
Keyword(s):  
Differential Diagnosis ◽  
Differential Expression ◽  
Focal Segmental Glomerulosclerosis ◽  
Minimal Change Disease ◽  
Bioinformatics Analysis ◽  
Minimal Change ◽  
Hub Genes ◽  
U6 Snrna ◽  
Segmental Glomerulosclerosis ◽  
Healthy Control

Abstract Background: Minimal change disease (MCD) and focal segmental glomerulosclerosis (FSGS) are common causes of nephrotic syndrome which have similar clinical as well as histologic magnification and hard to differentiate. This study aimed to identify novel biomarkers to distinguish FSGS and MCD through bioinformatics analysis and elucidate the possible molecular mechanism. Material and Methods: Based on the microarray datasets GSE104948 and GSE108113 downloaded from the Gene Expression Omnibus database, the differentially expressed genes (DEGs) between FSGS vs healthy control, MCD vs healthy control were identified, and further defined by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Hub genes were checked by protein-protein interaction networks. Results: A total of and 358 and 368 genes were identified in FSGS and MCD compared with healthy controls, among them, there were156 overlapping DEGs. GO analysis showed the DEGs in these two diseases were simultaneously enriched in mRNA splicing, RNA polymerase II transcription, mRNA export, insulin stimulus, integrin-mediated signaling pathway, viral process and phagocytosis. Module analysis showed that genes in the top 1 significant module of the PPI network were mainly associated with Spliceosome among FSGS and MCD. The top 10 hub genes analysis discovered that most of hub genes were same between two disease, while among these genes, CD2 cytoplasmic tail binding protein 2 (CD2BP2), U6 snRNA-associated Sm-like protein (LSM8) and Small nuclear ribonucleoprotein polypeptides B (SNRPB) only differential expression in FSGS and Splicing factor 3A, subunit 3 (SF3A3) only differential expression in MCD, which may be used for differential diagnosis of these two diseases in the future. Conclusions: We identified key genes and mainly pathway associated with FSGS and MCD. Our results provide a set of potential genes used for differential diagnosis of these two diseases.

scholarly journals Clinical manifestations of focal segmental glomerulosclerosis in Japan from the Japan Renal Biopsy Registry: age stratification and comparison with minimal change disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Takaya Ozeki ◽  
Shoichi Maruyama ◽  
Toshiyuki Imasawa ◽  
Takehiko Kawaguchi ◽  
Hiroshi Kitamura ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
Renal Biopsy ◽  
Focal Segmental Glomerulosclerosis ◽  
Clinical Diagnosis ◽  
Clinical Characteristics ◽  
Minimal Change Disease ◽  
Multivariable Analysis ◽  
Laboratory Data ◽  
Minimal Change ◽  
Segmental Glomerulosclerosis

AbstractFocal segmental glomerulosclerosis (FSGS) is a serious condition leading to kidney failure. We aimed to investigate the clinical characteristics of FSGS and its differences compared with minimal change disease (MCD) using cross-sectional data from the Japan Renal Biopsy Registry. In Analysis 1, primary FSGS (n = 996) were stratified by age into three groups: pediatric (< 18 years), adult (18–64 years), and elderly (≥ 65 years), and clinical characteristics were compared. Clinical diagnosis of nephrotic syndrome (NS) was given to 73.5% (97/132) of the pediatric, 41.2% (256/622) of the adult, and 65.7% (159/242) of the elderly group. In Analysis 2, primary FSGS (n = 306) and MCD (n = 1303) whose clinical diagnosis was nephrotic syndrome (NS) and laboratory data were consistent with NS, were enrolled. Logistic regression analysis was conducted to elucidate the variables which can distinguish FSGS from MCD. On multivariable analysis, higher systolic blood pressure, higher serum albumin, lower eGFR, and presence of hematuria associated with FSGS. In Japanese nationwide registry, primary FSGS patients aged 18–64 years showed lower rate of NS than those in other ages. Among primary nephrotic cases, FSGS showed distinct clinical features from MCD.

Pathogenesis of proteinuria in minimal change disease and focal segmental glomerulosclerosis

2018 ◽  
Author(s):  
Patrick Niaudet ◽  
Alain Meyrier
Keyword(s):  
Serum Albumin ◽  
Focal Segmental Glomerulosclerosis ◽  
Minimal Change Disease ◽  
Minimal Change ◽  
Slit Diaphragm ◽  
Segmental Glomerulosclerosis ◽  
Podocyte Loss ◽  
Glomerular Filtrate

It is now well established that the podocyte, and in particular the slit diaphragm structure, are critical to the barrier to serum albumin entering glomerular filtrate in large quantities. In minimal change disease there is proteinuria without podocyte death, whereas in focal segmental glomerulosclerosis there is not only podocyte dysfunction but also podocyte loss.

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