scholarly journals Study of lncRNA TUG1/miR-320 in EPC Transplantation to Improve Lower Limb Ischemia and Promote Angiogenesis in Mice

2021 ◽  
Author(s):  
Wei Qian ◽  
Qiong Yan ◽  
Xinmiao Jiang ◽  
Jungang Nie ◽  
Jiaqi He ◽  
...  
Keyword(s):  
Blood Flow ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Nude Mouse Model ◽  
Flow Measurements ◽  
Lower Limb Ischemia ◽  
Lower Limb Muscles ◽  
Blood Flow Measurements ◽  
Related Proteins ◽  
Lncrna Tug1

Abstract To The aim of the current study was to investigate the changes in lncRNA TUG1/miR-320 and related proteins with ischaemic time in an ischemia model. A nude mouse model of lower limb ischemia was established by ligating the femoral artery, and laser Doppler measurements were used to demonstrate the successful establishment of the ischemia model. The cells were extracted from the bone marrow of nude mice, and the proliferation, migration and vascular-forming ability of the cells were analysed. When transplanted into ischemia model mice, blood flow measurements indicated that EPCs can speed up blood flow recovery. The results of HE staining indicated an improvement in inflammatory damage, and immunohistochemistry revealed an increase in capillaries. RT-PCR and Western blot experiments showed that the improvement of ischemia was related to an increase in lncRNA TUG1 and a decrease in miR-320 and that the expression of the related downstream proteins STAT3, VEGFR-2, Wnt-5a and β-catenin increased gradually. These changes promoted an increase in capillaries, the recovery of blood flow, and the improvement of muscle damage. Therefore, EPC transplantation can improve the inflammatory response of lower limb muscles by increasing the expression of lncRNA TUG1 and thereby accelerate the recovery of ischaemic limbs.

scholarly journals Quality of life assessment in patients with acute limb ishemia

2020 ◽  
Vol 23 (2) ◽  
pp. 15-18
Author(s):  
Gennady V. Sinyavin ◽  
Aleksandr N. Kosenkov ◽  
Ivan A. Vinоkurоv ◽  
Gevorg V. Mnatsakanjan ◽  
Sanija N. Odinokova ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Blood Flow ◽  
Lower Limb ◽  
Short Form ◽  
Surgical Care ◽  
Limb Ischemia ◽  
Life Assessment ◽  
Quality Of Life Assessment ◽  
Lower Limb Ischemia

Background. In conditions of permanent progress in the quality of surgical care in various areas of surgery, it is very important to allow patients themselves to evaluate provided treatment. Aims: this paper analyzes the quality of life in individuals with acute lower limb ischemia who have undergone lower limb revascularization surgery. Materials and methods. An assessment of the quality of life of 122 patients with acute lower limb ischemia (ALI) of II degree who were treated at the Moscow Сity Сlinical Hospital оf S.S. Yudin from 2016 to 2018 (average age 72.1 14.7 years) was performed. The quality of life was assessed using the medical Outcomes Study-Short Form (MOS SF-36) quality of life assessment methodology. Results. When assessing the quality of life of patients, it was found that the psychological and physical components of patients health are significantly affected by the angiotropic effect of the affecting factor associated with the presence of diabetes mellitus. 1 month after surgical restoration of blood flow against the background of the initial embolism, 83.6% of the respondents who did not suffer from diabetes noted a persistent improvement in their General condition and quality of life. Conclusion. This method of assessing the quality of life after surgical restoration of blood flow in patients with acute lower limb ischemia can be used in angiosurgical practice and clearly reflects of patient satisfaction with the treatment.

Organ dysfunction after blood flow restoration in patients with acute lower limb ischemia

2019 ◽  
Vol 12 (5) ◽  
pp. 477
Author(s):  
Yu. V. Belov ◽  
G. V. Sinyavin ◽  
I. A. Vinokurov ◽  
G. V. Mnatsakanyan
Keyword(s):  
Blood Flow ◽  
Organ Dysfunction ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Lower Limb Ischemia ◽  
Flow Restoration

scholarly journals Comparison of Digital Subtraction Angiography Combined Arterial Thrombectomy Versus Simple Arterial Thrombectomy in the Treatment of Acute Lower Limb Ischemia

2021 ◽  
Author(s):  
Hongwei Ge ◽  
Bin Song ◽  
Xin Wang ◽  
Yunfeng Zhu ◽  
Yiming Huang ◽  
...  
Keyword(s):  
Blood Flow ◽  
Success Rate ◽  
Digital Subtraction Angiography ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Digital Subtraction ◽  
Lower Limb Ischemia ◽  
Primary Surgery ◽  
Group A ◽  
Group B

Abstract Background: This study aimed to compare the clinical efficacy of digital subtraction angiography (DSA) combined arterial thrombectomy versus simple arterial thrombectomy in the treatment of acute lower limb ischemia (ALI). Methods: This retrospective cohort study collected the clinical data from 125 patients (129 affected lower limbs) with ALI who underwent emergency surgery from March 2010 to November 2019. Patients were consecutively divided into Group A and Group B. Patients in Group A underwent simple arterial thrombectomy via the Fogarty catheterization. Patients in Group B underwent arterial thrombectomy, and the DSA was performed during the surgery. The differences in the success rate of primary surgery, the second intervention rate, and the amputation/mortality rate within 30-d after surgery were compared. Results: In Group A, 4 of 70 limbs (5.7%) were amputated, 54 of 70 limbs (77.1%) had improved blood flow, 14 of 70 limbs (20.0%) received a second intervention, and 3 of 68 patients (4.4%) died within 30 d. In Group B, 1 of 59 limbs (1.7%) was amputated, 56 of 59 limbs (94.9%) had improved blood flow, 3 of 59 limbs (5.1%) received a second intervention, and 2 of 57 patients (3.5%) died within 30-d. The success rate of primary surgery, the second intervention rate, and the amputation rate of Group B were significantly lower than Group A (P<0.05). Conclusion: Arterial thrombectomy combined with DSA may effectively improve the clinical efficacy of patients with ALI.

Role of Cilostazol in Rats with Diabetic Lower Limb Ischemia and Its Influences on Changes in Matrix Metallopeptidase-9 and Matrix Metalloproteinase-8

2020 ◽  
Vol 10 (3) ◽  
pp. 402-407
Author(s):  
Yao Tang ◽  
Jian Huang ◽  
Liming Shen ◽  
Zhanao Liu ◽  
Dayong Zhou
Keyword(s):  
Blood Flow ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Vascular Wall ◽  
Normal Group ◽  
The Other ◽  
Model Group ◽  
Lower Limb Ischemia

Objective: To assess cilostazol’s effect on rats with diabetic lower limb ischemia. Methods: 36 Sprague-Dawley rats were randomly separated into normal group (n = 12), model group (n = 12) and cilostazol group (n = 12). The model of diabetic lower limb ischemia was prepared and normal saline was intraperitoneally injected postoperatively into model group. Besides, cilostazol was administrated through intraperitoneal injection after establishment of model in cilostazol group. After intervention for 6 weeks, doppler blood flow imager was employed to detect blood flow, and the expressions of MMP-8 and MMP-9 were assessed via immunohistochemistry, western blotting or quantitative polymerase chain reaction (qPCR). TUNEL assay was performed to evaluate cell apop-tosis. Results: Doppler blood flow imager showed that compared with normal group, perfusion unit (PU) index was significantly reduced in the other two groups (P < 0.05) with higher value in cilosta-zol group (P < 0.05). The immunohistochemistry results revealed that in comparison with those in normal group, MMP-8 and MMP-9 positive staining was significantly elevated in the other two groups (P < 0.05) with higher level in cilostazol group than model group. In addition, MMP-8 and MMP-9 expressions were significantly elevated in other two groups (P < 0.05), with higher level in cilostazol group (P < 0.05). Moreover, the vascular wall smooth muscle cells in the other two groups had a significantly elevated apoptosis rate (P < 0.05) with higher apoptosis in cilostazol group (P < 0.05). Conclusion: Cilostazol up-regulates MMP-8 and MMP-9 to increase the apoptosis of vascular wall smooth muscle cells, thereby improving the blood flow in rats with diabetic lower limb ischemia.

scholarly journals Comparison of digital subtraction angiography combined arterial thrombectomy versus simple arterial thrombectomy in the treatment of acute lower limb ischemia

BMC Surgery ◽  
2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Hongwei Ge ◽  
Bin Song ◽  
Xin Wang ◽  
Yunfeng Zhu ◽  
Yiming Huang ◽  
...  
Keyword(s):  
Blood Flow ◽  
Success Rate ◽  
Digital Subtraction Angiography ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Digital Subtraction ◽  
Lower Limb Ischemia ◽  
Primary Surgery ◽  
Group A ◽  
Group B

Abstract Background This study aimed to compare the clinical efficacy of digital subtraction angiography (DSA) combined arterial thrombectomy versus simple arterial thrombectomy in the treatment of acute lower limb ischemia (ALI). Methods This retrospective cohort study collected the clinical data from 124 patients (128 affected lower limbs) with ALI who underwent emergency surgery from March 2010 to November 2019. Patients were consecutively divided into Group A and Group B. Patients in Group A underwent simple arterial thrombectomy via the Fogarty catheterization. Patients in Group B underwent arterial thrombectomy, and the DSA was performed during the surgery. The differences in the success rate of primary surgery, the second intervention rate, and the amputation/mortality rate within 30-days after surgery were compared. Results In Group A, 4 of 70 limbs (5.7%) were amputated, 54 of 70 limbs (77.1%) had improved blood flow, 14 of 70 limbs (20.0%) received a second intervention, and 3 of 68 patients (4.4%) died within 30 days. In Group B, 1 of 58 limbs (1.7%) was amputated, 56 of 58 limbs (96.6%) had improved blood flow, 3 of 58 limbs (5.2%) received a second intervention, and 2 of 56 patients (3.5%) died within 30-days. The success rate of primary surgery, the second intervention rate, and the amputation rate of Group B were significantly lower than Group A (P < 0.05). Conclusion Arterial thrombectomy combined with DSA may effectively improve the clinical efficacy of patients with ALI.

scholarly journals Foot Skin Blood Flow Following Infrainguinal Revascularization for Critical Lower Limb Ischemia

2006 ◽  
Vol 31 (4) ◽  
pp. 401-406 ◽  
Author(s):  
F. Saucy ◽  
B. Dischl ◽  
A. Delachaux ◽  
F. Feihl ◽  
L. Liaudet ◽  
...  
Keyword(s):  
Blood Flow ◽  
Skin Blood Flow ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Lower Limb Ischemia

scholarly journals Pathogenesis of ischemia/reperfusion syndrome

2018 ◽  
Vol 99 (4) ◽  
pp. 640-644 ◽  
Author(s):  
D E Kutepov ◽  
M S Zhigalova ◽  
I N Pasechnik
Keyword(s):  
Blood Flow ◽  
Lower Limb ◽  
Ischemia Reperfusion ◽  
Vascular Complications ◽  
Limb Ischemia ◽  
Biologically Active ◽  
Lower Limbs ◽  
Ion Pump ◽  
Critical Ischemia ◽  
Lower Limb Ischemia

Ischemia/reperfusion syndrome is a collective concept that combines various pathological conditions developing against the background of the restoration of the main blood flow in the organ or limb segment, which has long been subjected to ischemia or traumatic amputation. Intensive care physicians often see ischemia/reperfusion syndrome after restoration of blood supply in patients with critical ischemia of the lower limb. The rate of critical ischemia of the lower limbs ranges from 400 to 1000 cases per 1 million of the population. The number of lower limb amputations due to critical lower limb ischemia in economically developed countries is 13.7-32.3 cases per 100,000 of the population. The main etiological factors of critical lower limb ischemia are atherosclerosis of peripheral vessels and vascular complications of diabetes. The pathogenesis of ischemia/reperfusion syndrome is based on a complex of pathophysiological changes resulting from the restoration of blood flow in previously ischemic lower limb. Restoration of blood circulation leads to massive flow into the systemic bloodstream of anaerobic metabolism products, free myoglobin, biologically active substances and inflammatory mediators. The main sources of reperfusion damage are activated forms of oxygen, in particular, superoxide radical О2-, nitric oxide, lipid peroxidation products. In the conditions of primary ischemia, and then tissue reperfusion, excessive production of activated oxygen forms leads to damage of biological structures (lipids, proteins, deoxyribonucleic acid), which causes disruption of normal cell functioning or its death due to necrosis or apoptosis, ion pump dysfunction, adhesion of leukocytes and increased vascular permeability.

Cardiac troponin I in patients with acute upper and lower limb ischemia

VASA ◽  
2008 ◽  
Vol 37 (4) ◽  
pp. 327-332 ◽  
Author(s):  
Koutouzis ◽  
Sfyroeras ◽  
Moulakakis ◽  
Kontaras ◽  
Nikolaou ◽  
...  
Keyword(s):  
Upper Limb ◽  
Lower Limb ◽  
Cardiac Troponin ◽  
Cardiac Involvement ◽  
Troponin I ◽  
Limb Ischemia ◽  
Elevated Troponin ◽  
Troponin Elevation ◽  
Lower Limb Ischemia ◽  
Ischemia Group

Background: The aim of this study was to investigate the presence, etiology and clinical significance of elevated troponin I in patients with acute upper or lower limb ischemia. The high sensitivity and specificity of cardiac troponin for the diagnosis of myocardial cell damage suggested a significant role for troponin in the patients investigated for this condition. The initial enthusiasm for the diagnostic potential of troponin was limited by the discovery that elevated cardiac troponin levels are also observed in conditions other than acute myocardial infarction, even conditions without obvious cardiac involvement. Patients and Methods: 71 consecutive patients participated in this study. 31 (44%) of them were men and mean age was 75.4 ± 10.3 years (range 44–92 years). 60 (85%) patients had acute lower limb ischemia and the remaining (11; 15%) had acute upper limb ischemia. Serial creatine kinase (CK), isoenzyme MB (CK-MB) and troponin I measurements were performed in all patients. Results: 33 (46%) patients had elevated peak troponin I (> 0.2 ng/ml) levels, all from the lower limb ischemia group (33/60 vs. 0/11 from the acute upper limb ischemia group; p = 0.04). Patients with lower limb ischemia had higher peak troponin I values than patients with upper limb ischemia (0.97 ± 2.3 [range 0.01–12.1] ng/ml vs. 0.04 ± 0.04 [0.01–0.14] ng/ml respectively; p = 0.003), higher peak CK values (2504 ± 7409 [range 42–45 940] U/ml vs. 340 ± 775 [range 34–2403] U/ml, p = 0.002, respectively, in the two groups) and peak CK-MB values (59.4 ± 84.5 [range 12–480] U/ml vs. 21.2 ± 9.1 [range 12–39] U/ml, respectively, in the two groups; p = 0.04). Peak cardiac troponin I levels were correlated with peak CK and CK-MB values. Conclusions: Patients with lower limb ischemia often have elevated troponin I without a primary cardiac source; this was not observed in patients presenting with acute upper limb ischemia. It is very important for these critically ill patients to focus on the main problem of acute limb ischemia and to attempt to treat the patient rather than the troponin elevation per se. Cardiac troponin elevation should not prevent physicians from providing immediate treatment for limb ischaemia to these patients, espescially when signs, symptoms and electrocardiographic findings preclude acute cardiac involvement.

Acute limb ischemia in a patient with pre-fibrotic myelofibrosis complicated by heparin-induced thrombocytopenia and thrombosis – case report and systematic review of dabigatran use

VASA ◽  
2020 ◽  
pp. 1-6 ◽  
Author(s):  
Marina Di Pilla ◽  
Stefano Barco ◽  
Clara Sacco ◽  
Giovanni Barosi ◽  
Corrado Lodigiani
Keyword(s):  
Systematic Review ◽  
Case Report ◽  
Lower Limb ◽  
Limb Ischemia ◽  
Acute Limb Ischemia ◽  
Review Of The Literature ◽  
Heparin Induced Thrombocytopenia ◽  
Lower Limb Ischemia ◽  
Vein Thrombosis ◽  
Left Lower Limb

Summary: A 49-year-old man was diagnosed with pre-fibrotic myelofibrosis after acute left lower-limb ischemia requiring amputation and portal vein thrombosis. After surgery he developed heparin-induced thrombocytopenia (HIT) with venous thromboembolism, successfully treated with argatroban followed by dabigatran. Our systematic review of the literature supports the use of dabigatran for suspected HIT.

Sign in / Sign up

Export Citation Format

Share Document