scholarly journals Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA)

2021 ◽  
Author(s):  
Meriem Bejaoui ◽  
Aprill Kee Oliva ◽  
May Sin Ke ◽  
Farhana Ferdousi ◽  
Hiroko Isoda
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
In Vitro Model ◽  
3D Model ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Caffeoylquinic Acid ◽  
Vitro Model

Abstract IntroductionDermal papilla cells (DPc) is an important element in studying the hair follicle (HF) niche. The human hair follicle dermal papilla cells (HFDPC) are widely used as an in vitro model to study hair growth related research. These cells are usually grown in 2D culture, nevertheless, this system did not show efficient therapeutic effect on HF regeneration and growth, and key differences were observed between cell activity in vitro and in vivo. ObjectiveRecent studies have showed that HFDPC grown in 3D hanging spheroids is more morphologically akin to intact DPc microenvironment. This current study showed that the 3D model is applicable to the commercial cell line with new insights on its variability by comparing to previous studies of gene signature restored by 3D culture.Methods and Results Our data demonstrated that HFDPCS grown in 3D in vitro model can influence not only hair growth-related pathways but also immune system -related pathways compared to 2D cell monolayer. Furthermore, we compared the expression of signalling molecules and metabolism-associated proteins of HFDPC in minoxidil (FDA approved drug for hair loss treatment) and 3,4,5-tri-O-caffeoylquinic acid (TCQA) (recently found to induce hair growth in vitro and in vivo) treated 3D and 2D cell cultures using microarray analysis. Conclusion Further validation of the results confirms the suitability of this cell line for 3D model while providing new insights such as to the mechanisms behind the hair growth effects of 3D spheroid treated with hair growth promoting agents.

scholarly journals The Effect of JAK Inhibitor on the Survival, Anagen Re-Entry, and Hair Follicle Immune Privilege Restoration in Human Dermal Papilla Cells

2020 ◽  
Vol 21 (14) ◽  
pp. 5137
Author(s):  
Jung Eun Kim ◽  
Yu Jin Lee ◽  
Hye Ree Park ◽  
Dong Geon Lee ◽  
Kwan Ho Jeong ◽  
...  
Keyword(s):  
Growth Factors ◽  
Hair Follicle ◽  
Dermal Papilla ◽  
Immune Privilege ◽  
Jak Inhibitors ◽  
Dermal Papilla Cells ◽  
Ifn Γ ◽  
Vitro Model ◽  
Stat Pathway

Topical or systemic administration of JAK inhibitors has been shown to be a new treatment modality for severe alopecia areata (AA). Some patients show a good response to JAK inhibitors, but frequently relapse after cessation of the treatment. There have been no guidelines about the indications and use of JAK inhibitors in treating AA. The basic pathomechanism of AA and the relevant role of JAK inhibitors should support how to efficiently use JAK inhibitors. We sought to investigate the effect of JAK1/2 inhibitor on an in vitro model of AA and to examine the possible mechanisms. We used interferon gamma-pretreated human dermal papilla cells (hDPCs) as an in vitro model of AA. Ruxolitinib was administered to the hDPCs, and cell viability was assessed. The change of expression of the Wnt/β-catenin pathway, molecules related to the JAK-STAT pathway, and growth factors in ruxolitinib-treated hDPCs was also examined by reverse transcription PCR and Western blot assay. We examined immune-privilege-related molecules by immunohistochemistry in hair-follicle culture models. Ruxolitinib did not affect the cell viability of the hDPCs. Ruxolitinib activated several molecules in the Wnt/β-catenin signaling pathway, including Lef1 and β-catenin, and suppressed the transcription of DKK1 in hDPCs, but not its translation. Ruxolitinib reverted IFN-γ-induced expression of caspase-1, IL-1β, IL-15, and IL-18, and stimulated several growth factors, such as FGF7. Ruxolitinib suppressed the phosphorylation of JAK1, JAK2 and JAK3, and STAT1 and 3 compared to IFN-γ pretreated hDPCs. Ruxolitinib pretreatment showed a protective effect on IFN-γ-induced expression of MHC-class II molecules in cultured hair follicles. In conclusion, ruxolitinib modulated and reverted the interferon-induced inflammatory changes by blocking the JAK-STAT pathway in hDPCs under an AA-like environment. Ruxolitinib directly stimulated anagen-re-entry signals in hDPCs by affecting the Wnt/β-catenin pathway and promoting growth factors in hDPCs. Ruxolitinib treatment prevented IFN-γ-induced collapse of hair-follicle immune privilege.

scholarly journals HNG, A Humanin Analogue, Promotes Hair Growth by Inhibiting Anagen-to-Catagen Transition

2020 ◽  
Vol 21 (12) ◽  
pp. 4553
Author(s):  
Sung Min Kim ◽  
Jung-Il Kang ◽  
Hoon-Seok Yoon ◽  
Youn Kyung Choi ◽  
Ji Soo Go ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Cell Cycle Progression ◽  
Dermal Papilla ◽  
Follicle Cell ◽  
Hair Shaft ◽  
Promoting Effect ◽  
Dermal Papilla Cells ◽  
Anagen Phase

The hair follicle goes through repetitive cycles including anagen, catagen, and telogen. The interaction of dermal papilla cells (DPCs) and keratinocytes regulates the hair cycle and hair growth. Humanin was discovered in the surviving brain cells of patients with Alzheimer’s disease. HNG, a humanin analogue, activates cell growth, proliferation, and cell cycle progression, and it protects cells from apoptosis. This study was performed to investigate the promoting effect and action mechanisms of HNG on hair growth. HNG significantly increased DPC proliferation. HNG significantly increased hair shaft elongation in vibrissa hair follicle organ culture. In vivo experiment showed that HNG prolonged anagen duration and inhibited hair follicle cell apoptosis, indicating that HNG inhibited the transition from the anagen to catagen phase mice. Furthermore, HNG activated extracellular signal-regulated kinase (Erk)1/2, Akt, and signal transducer and activator of transcription (Stat3) within minutes and up-regulated vascular endothelial growth factor (VEGF) levels on DPCs. This means that HNG could induce the anagen phase longer by up-regulating VEGF, which is a Stat3 target gene and one of the anagen maintenance factors. HNG stimulated the anagen phase longer with VEGF up-regulation, and it prevented apoptosis by activating Erk1/2, Akt, and Stat3 signaling.

scholarly journals Transcriptome Profiling of Human Follicle Dermal Papilla Cells in response to Porphyra-334 Treatment by RNA-Seq

2021 ◽  
Vol 2021 ◽  
pp. 1-15
Author(s):  
Su Yeon Kim ◽  
Won Kyong Cho ◽  
Hye-In Kim ◽  
Seung Hye Paek ◽  
Sung Joo Jang ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Transcriptome Profiling ◽  
Dermal Papilla ◽  
Clinical Test ◽  
Dermal Papilla Cells ◽  
Epidermal Structure ◽  
Vitro Assay

Porphyra-334 is a kind of mycosporine-like amino acid absorbing ultraviolet-A. Here, we characterized porphyra-334 as a potential antiaging agent. An in vitro assay revealed that porphyra-334 dramatically promoted collagen synthesis in fibroblast cells. The effect of porphyra-334 on cell proliferation was dependent on the cell type, and the increase of cell viability by porphyra-334 was the highest in keratinocyte cells among the three tested cell types. An in vivo clinical test with 22 participants demonstrated the possible role of porphyra-334 in the improvement of periorbital wrinkles. RNA-sequencing using human follicle dermal papilla (HFDP) cells upon porphyra-334 treatment identified the upregulation of metallothionein- (MT-) associated genes, confirming the antioxidant role of porphyra-334 with MT. Moreover, the expression of genes involved in nuclear chromosome segregation and the encoding of components of kinetochores was upregulated by porphyra-334 treatment. Furthermore, we found that several genes associated with the hair follicle cycle, the hair follicle structure, the epidermal structure, and stem cells were upregulated by porphyra-334 treatment, suggesting the potential role of porphyra-334 in hair follicle growth and maintenance. In summary, we provided several new pieces of evidence of porphyra-334 as a potential antiaging cosmetic agent and elucidated the expression network in HFDP cells upon porphyra-334.

scholarly journals Hair follicle-derived mesenchymal stem cells decrease alopecia areata mouse hair loss and reduce inflammation around the hair follicle

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Weiyue Deng ◽  
Yuying Zhang ◽  
Wei Wang ◽  
Aishi Song ◽  
Omar Mukama ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hair Follicle ◽  
Hair Loss ◽  
Alopecia Areata ◽  
In Vitro Model ◽  
Ifn Γ ◽  
Vitro Model

Abstract Background Alopecia areata (AA) is a common autoimmune hair loss disease with increasing incidence. Corticosteroids are the most widely used for hair loss treatment; however, long-term usage of hormonal drugs is associated with various side effects. Mesenchymal stem cells (MSCs) therapy has been studied extensively to curb autoimmune diseases without affecting immunity against diseases. Methods Hair follicle-derived MSCs (HF-MSCs) were harvested from the waste material of hair transplants, isolated and expanded. The therapeutic effect of HF-MSCs for AA treatment was investigated in vitro AA-like hair follicle organ model and in vivo C3H/HeJ AA mice model. Results AA-like hair follicle organ in vitro model was successfully established by pre-treatment of mouse vibrissa follicles by interferon-γ (IFN-γ). The AA-like symptoms were relieved when IFN-γ induced AA in vitro model was co-cultured with HF-MSC for 2 days. In addition, when skin grafted C3H/HeJ AA mice models were injected with 106 HF-MSCs once a week for 3 weeks, the transcription profiling and immunofluorescence analysis depicted that HF-MSCs treatment significantly decreased mouse hair loss and reduced inflammation around HF both in vitro and in vivo. Conclusions This study provides a new therapeutic approach for alopecia areata based on HF-MSCs toward its future clinical application.

Clinical, Trichoscopic And Immunohistochemical Evaluation of Autologous Adipose Derived Adult Stem Cell Injection in the Treatment of Female Pattern Hair Loss

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Hoda Ahmed Moneib ◽  
Ghada Fathy Mohamed ◽  
Naglaa Samir Ahmed ◽  
Mahy El-Bassiouny El-Sayed Abou-Noor
Keyword(s):  
Hair Follicle ◽  
Hair Loss ◽  
Adult Stem Cells ◽  
Adult Stem Cell ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Dermal Papilla Cells ◽  
Female Pattern Hair Loss

Abstract Background Cellular and cell-derived components of adipose-derived tissue for the purposes of dermatologic and aesthetic rejuvenation applications have become increasingly studied and integrated into clinical practice. The hair follicle goes through phases of growth, regression, and quiescence, and it is suspected that adipocytes secrete factors to promote activation of hair follicles dermal papilla cells, increasing migration, and proliferation in vitro; as well as increasing conversion of hair follicles from the telogen to anagen phase in vivo. Objectives Evaluation of efficacy and safety of adipose-derived adult stem cells (ADSCs) injection in hair follicle regeneration in female pattern hair loss (FPHL). Methods 33 patients were included and divided into 3 groups according to Sinclair’s classification according to severity. ADSCs were extracted from lipoaspirate and injected into the frontoparietal scalp. Patients were assessed clinically, trichoscopically and immunohistochemically. Results At week 24, there was improvement of hair thickness and count, both in frontal and occipital areas. Histopathological and immunohistochemical assessment at week 12 showed decrease of perifollicular inflammation and decrease of DKK-1 immunostaining. Conclusion The use of ADSCs in treatment of FPHL in subjects included in this study showed improvement of perifollicular inflammation, in addition to density and thickness of hair.

scholarly journals In Vitro Assessment of Biofield Energy Healing Treatment on Hair Growth by Enhanced Proliferation of Human Follicular Dermal Papilla Cells (HFDPCs)

2018 ◽  
Vol 4 (1) ◽  
pp. 30-44
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Promoter ◽  
Androgenic Alopecia ◽  
Dermal Papilla Cells ◽  
Test Items ◽  
Energy Healing ◽  
The One

Patterned hair loss or androgenic alopecia is a disorder affecting millions of peoples, in which hair sheds without adequate regrowth. Authors propose a new approach to increase hair growth. In this study, authors examined the in vitro potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test items, Dulbecco’s Modified Eagle Medium (DMEM) on the Human Follicular Dermal Papilla Culture Cells (HFDPC) for the assessment of growth and development. The test items (DMEM) were divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third parts were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. The Biofield Energy Healing Treatment was provided by a renowned Biofield Energy Healer (The Trivedi Effect®), remotely for ~5 minutes under standard laboratory conditions through unique energy transmission process. The experimental results showed that one-time Biofield Energy Treated DMEM showed 70.24% and two-times Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased the proliferation of dermal papilla cells by 207.62% as compared to the untreated DMEM group. The overall results demonstrated that the Biofield Energy Healing Treatment significantly increased the proliferation of human hair follicle dermal papilla cells in vitro. Therefore, the Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter in vivo against different types of skin injuries, hair disorders, and opens new research venues.

Sign in / Sign up

Export Citation Format

Share Document