scholarly journals In Vitro Assessment of Biofield Energy Healing Treatment on Hair Growth by Enhanced Proliferation of Human Follicular Dermal Papilla Cells (HFDPCs)

2018 ◽  
Vol 4 (1) ◽  
pp. 30-44
Author(s):  
Dahryn Trivedi ◽  
Snehasis Jana
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Growth Promoter ◽  
Androgenic Alopecia ◽  
Dermal Papilla Cells ◽  
Test Items ◽  
Energy Healing ◽  
The One

Patterned hair loss or androgenic alopecia is a disorder affecting millions of peoples, in which hair sheds without adequate regrowth. Authors propose a new approach to increase hair growth. In this study, authors examined the in vitro potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test items, Dulbecco’s Modified Eagle Medium (DMEM) on the Human Follicular Dermal Papilla Culture Cells (HFDPC) for the assessment of growth and development. The test items (DMEM) were divided into three parts. First part did not receive any sort of treatment and defined as the untreated DMEM group. The second and third parts were treated with the one-time and two-times Biofield Energy Treatment by a renowned Biofield Energy Healer, Dahryn Trivedi and coded as the one-time Biofield Energy Treated DMEM (BT-I) and two-times Biofield Energy Treated DMEM (BT-II) groups, respectively. The Biofield Energy Healing Treatment was provided by a renowned Biofield Energy Healer (The Trivedi Effect®), remotely for ~5 minutes under standard laboratory conditions through unique energy transmission process. The experimental results showed that one-time Biofield Energy Treated DMEM showed 70.24% and two-times Biofield Energy Treated DMEM significantly (p ≤ 0.001) increased the proliferation of dermal papilla cells by 207.62% as compared to the untreated DMEM group. The overall results demonstrated that the Biofield Energy Healing Treatment significantly increased the proliferation of human hair follicle dermal papilla cells in vitro. Therefore, the Consciousness Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter in vivo against different types of skin injuries, hair disorders, and opens new research venues.

scholarly journals Establishment of 3D Spheroid Human Dermal Papilla Cells as an Effective Model for Hair Growth Screening Compounds Compared with the 2D System: An Example of Minoxidil and 3,4,5-Tri-O-caffeoylquinic acid (TCQA)

2021 ◽  
Author(s):  
Meriem Bejaoui ◽  
Aprill Kee Oliva ◽  
May Sin Ke ◽  
Farhana Ferdousi ◽  
Hiroko Isoda
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
In Vitro Model ◽  
3D Model ◽  
Dermal Papilla ◽  
Dermal Papilla Cells ◽  
Caffeoylquinic Acid ◽  
Vitro Model

Abstract IntroductionDermal papilla cells (DPc) is an important element in studying the hair follicle (HF) niche. The human hair follicle dermal papilla cells (HFDPC) are widely used as an in vitro model to study hair growth related research. These cells are usually grown in 2D culture, nevertheless, this system did not show efficient therapeutic effect on HF regeneration and growth, and key differences were observed between cell activity in vitro and in vivo. ObjectiveRecent studies have showed that HFDPC grown in 3D hanging spheroids is more morphologically akin to intact DPc microenvironment. This current study showed that the 3D model is applicable to the commercial cell line with new insights on its variability by comparing to previous studies of gene signature restored by 3D culture.Methods and Results Our data demonstrated that HFDPCS grown in 3D in vitro model can influence not only hair growth-related pathways but also immune system -related pathways compared to 2D cell monolayer. Furthermore, we compared the expression of signalling molecules and metabolism-associated proteins of HFDPC in minoxidil (FDA approved drug for hair loss treatment) and 3,4,5-tri-O-caffeoylquinic acid (TCQA) (recently found to induce hair growth in vitro and in vivo) treated 3D and 2D cell cultures using microarray analysis. Conclusion Further validation of the results confirms the suitability of this cell line for 3D model while providing new insights such as to the mechanisms behind the hair growth effects of 3D spheroid treated with hair growth promoting agents.

scholarly journals Assessment of Hair Growth Treatment with the Consciousness Energy Healing Treated Williams Medium E Using Mouse Vibrissae Hair Follicle Organ Culture

2019 ◽  
Vol 1 (3) ◽  
pp. 12-19
Author(s):  
Mahendra Kumar Trivedi ◽  
Snehasis Jana
Keyword(s):  
Organ Culture ◽  
Hair Follicle ◽  
Test Item ◽  
Hair Growth ◽  
Sexual Communication ◽  
Growth Promotion ◽  
Growth Promoter ◽  
Energy Healing ◽  
Hair Follicle Organ Culture

Hair is playing an interesting part in human for social and sexual communication. Loss of hair follicle leads to various skin disorders. For this consequence, the present study has investigated the potential of the Biofield Energy Healing (The Trivedi Effect®) Treated test item (William’s Medium E) on the vibrissae hair follicle organ culture cells for the assessment of hair cell growth and development in vitro. The test item was divided into two parts. One part was defined as the untreated test item, where no Biofield Energy Treatment provided, while the other part was defined as the Biofield Energy Treated test item, which received the Biofield Energy Healing Treatment by renowned Biofield Energy Healer, Mahendra Kumar Trivedi. The study parameters like bulb thickness and formation of telogen were assessed using cell-based assay with the help of UTHSCSA Image tool version 3. The experimental results showed that the untreated test item group showed 20.9% and 28.2% increased bulb thickness on day 5 and 7, respectively compared to the day 1, while did not produce telogen follicles upto day 7. Besides, the percentage of telogen follicle was found as 43%, 57%, and 71% on day 3, 5, and 7, respectively of the Biofield Energy Treated test item group compared to the day 1. The overall results demonstrated that the Biofield Energy Treatment has the potential for hair growth promotion as evident via increased the formation of telogen. Therefore, the Biofield Energy Healing (The Trivedi Effect®) Treatment might be useful as a hair growth promoter for various treatment of skin injuries and skin-related disorders like necrotizing fasciitis, actinic keratosis, sebaceous cysts, diaper rash, decubitus ulcer etc.

scholarly journals HNG, A Humanin Analogue, Promotes Hair Growth by Inhibiting Anagen-to-Catagen Transition

2020 ◽  
Vol 21 (12) ◽  
pp. 4553
Author(s):  
Sung Min Kim ◽  
Jung-Il Kang ◽  
Hoon-Seok Yoon ◽  
Youn Kyung Choi ◽  
Ji Soo Go ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Cell Cycle Progression ◽  
Dermal Papilla ◽  
Follicle Cell ◽  
Hair Shaft ◽  
Promoting Effect ◽  
Dermal Papilla Cells ◽  
Anagen Phase

The hair follicle goes through repetitive cycles including anagen, catagen, and telogen. The interaction of dermal papilla cells (DPCs) and keratinocytes regulates the hair cycle and hair growth. Humanin was discovered in the surviving brain cells of patients with Alzheimer’s disease. HNG, a humanin analogue, activates cell growth, proliferation, and cell cycle progression, and it protects cells from apoptosis. This study was performed to investigate the promoting effect and action mechanisms of HNG on hair growth. HNG significantly increased DPC proliferation. HNG significantly increased hair shaft elongation in vibrissa hair follicle organ culture. In vivo experiment showed that HNG prolonged anagen duration and inhibited hair follicle cell apoptosis, indicating that HNG inhibited the transition from the anagen to catagen phase mice. Furthermore, HNG activated extracellular signal-regulated kinase (Erk)1/2, Akt, and signal transducer and activator of transcription (Stat3) within minutes and up-regulated vascular endothelial growth factor (VEGF) levels on DPCs. This means that HNG could induce the anagen phase longer by up-regulating VEGF, which is a Stat3 target gene and one of the anagen maintenance factors. HNG stimulated the anagen phase longer with VEGF up-regulation, and it prevented apoptosis by activating Erk1/2, Akt, and Stat3 signaling.

scholarly journals Preclinical and Clinical Studies Demonstrate That the Proprietary Herbal Extract DA-5512 Effectively Stimulates Hair Growth and Promotes Hair Health

2017 ◽  
Vol 2017 ◽  
pp. 1-11 ◽  
Author(s):  
Jae Young Yu ◽  
Biki Gupta ◽  
Hyoung Geun Park ◽  
Miwon Son ◽  
Joon-Ho Jun ◽  
...  
Keyword(s):  
Clinical Study ◽  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Follicles ◽  
Herbal Extract ◽  
Control Group ◽  
Dependent Manner ◽  
Ki 67 ◽  
Dermal Papilla Cells

The proprietary DA-5512 formulation comprises six herbal extracts from traditional oriental plants historically associated with therapeutic and other applications related to hair. Here, we investigated the effects of DA-5512 on the proliferation of human dermal papilla cells (hDPCs) in vitro and on hair growth in C57BL/6 mice and conducted a clinical study to evaluate the efficacy and safety of DA-5512. DA-5512 significantly enhanced the viability of hDPCs in a dose-dependent manner (p<0.05), and 100 ppm of DA-5512 and 1 μM minoxidil (MXD) significantly increased the number of Ki-67-positive cells, compared with the control group (p<0.05). MXD (3%) and DA-5512 (1%, 5%) significantly stimulated hair growth and increased the number and length of hair follicles (HFs) versus the controls (each p<0.05). The groups treated with DA-5512 exhibited hair growth comparable to that induced by MXD. In clinical study, we detected a statistically significant increase in the efficacy of DA-5512 after 16 weeks compared with the groups treated with placebo or 3% MXD (p<0.05). In conclusion, DA-5512 might promote hair growth and enhance hair health and can therefore be considered an effective option for treating hair loss.

scholarly journals Vibrissa dermal papilla cell aggregative behaviour in vivo and in vitro

Development ◽  
1984 ◽  
Vol 79 (1) ◽  
pp. 211-224
Author(s):  
Colin A. B. Jahoda ◽  
Roy F. Oliver
Keyword(s):  
Dermal Papilla ◽  
Experimental Period ◽  
Cell Types ◽  
Hair Follicles ◽  
Skin Fibroblasts ◽  
Dermal Papilla Cells ◽  
Adult Rat

Parallel cultures of adult rat vibrissa dermal papilla cells and skin fibroblasts revealed differences between the two cell types with respect to a number of criteria. In particular the dermal papilla cells demonstrated a distinctive single cell morphology, and at confluence formed cell aggregates radically different from regular fibroblast multilayering and patterning. This finding confirmed repeated observations of papilla cell clumping in short-term culture. The dermal papilla cells which are mitotically quiescent in situ were also shown to have a lower proliferative capacity than the skin fibroblasts. The affinity shown by papilla cells towards each other in culture reflected the behaviour demonstrated by isolated dermal papillae transplanted into ear dermis and into the collagenous capsule of the vibrissa follicle. In the absence of epidermal contact the papilla cells remained as recognizable rounded aggregates for the experimental period of up to nine months. Synthesis of extracellular material typical of that seen in situ was observed, particularly during the first weeks following transplantation. The collective behaviour of the dermal papilla cells revealed in this study may be significant for the morphogenetic activity of the papilla, and for papilla size during the hair cycle. It may also reflect the retention of embryonic-like properties by the dermal component of adult hair follicles.

scholarly journals Nature-derived lignan compound VB-1 exerts hair growth-promoting effects by augmenting Wnt/β-catenin signaling in human dermal papilla cells

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4737 ◽  
Author(s):  
Jieshu Luo ◽  
Mengting Chen ◽  
Yingzi Liu ◽  
Hongfu Xie ◽  
Jian Yuan ◽  
...  
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Hair Shaft ◽  
Cell Counting ◽  
Dependent Manner ◽  
Rt Pcr ◽  
Concentration Dependent Manner ◽  
Dermal Papilla Cells ◽  
Growth Promoting

Background Vitexin is a kind of lignan compound which has been shown to possess a variety of pharmacological effects, such as anti-inflammatory, anti-oxidative and anti-cancer activities. However the effect of vitexin on hair regeneration has not been elaborated. Methods The proliferation of human dermal papilla cells (hDPCs) was examined by cell counting and continuous cell culture after vitexin compound 1 (VB-1) was treated. The expression of lef1, wnt5a, bmp2, bmp4, alpl and vcan was examined by RT-PCR. The expression of dkk1, tgf-β1, active-β-Catenin, and AXIN2 was examined by RT-PCR or immunoblotting. Hair shaft growth was measured in the absence or presence of VB-1. Results We demonstrated that VB-1 significantly promotes the proliferation of hDPCs in a concentration-dependent manner within a certain concentration range. Among the hair growth-related genes investigated, dkk1 was clearly down-regulated in hDPCs treated with VB-1. The increased active β-Catenin and decreased AXIN2 protein levels suggest that VB-1 facilitates Wnt/β-catenin signaling in hDPCs in vitro. The expression of DP signature genes was also upregulated after VB-1 treatment. Our study further indicated that VB-1 promotes human hair follicle (HF) growth by HF organ culture assay. Discussion VB-1 may exert hair growth-promoting effects via augmenting Wnt/β-catenin signaling in hDPCs.

scholarly journals Review of Hair Follicle Dermal Papilla cells as in vitro screening model for hair growth

2018 ◽  
Vol 40 (5) ◽  
pp. 429-450 ◽  
Author(s):  
Alka Madaan ◽  
Ritu Verma ◽  
Anu T. Singh ◽  
Manu Jaggi
Keyword(s):  
Hair Follicle ◽  
Hair Growth ◽  
Dermal Papilla ◽  
In Vitro Screening ◽  
Dermal Papilla Cells ◽  
Screening Model

scholarly journals Broussonetia papyrifera Promotes Hair Growth Through the Regulation of β-Catenin and STAT6 Target Proteins: A Phototrichogram Analysis of Clinical Samples

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 40
Author(s):  
Young Han Lee ◽  
Gaewon Nam ◽  
Myong-Ki Kim ◽  
Seok-Cheol Cho ◽  
Bu Young Choi
Keyword(s):  
Hair Growth ◽  
Dermal Papilla ◽  
Interleukin 4 ◽  
Clinical Samples ◽  
Broussonetia Papyrifera ◽  
Dermal Papilla Cells ◽  
Target Proteins ◽  
Start Date ◽  
Growth Assay

Broussonetia papyrifera (B.papyrifera), belonging to the Moraceae family, is known to elicit anti-inflammatory, antioxidant, anti-tyrosinase, anticancer, antinociceptive, and antimicrobial effects. The present study has been designed to examine the effects of B. papyrifera extract on hair growth through in vitro and clinical samples. Real-time cell growth assay, T-cell factor/lymphoid enhancer-binding factor (TCF/LEF), activation of signal transducer and activator of transcription-6(STAT6) and STAT3 reporter gene function, and Western blotting was performed to examine whether B. papyrifera regulates the expression of target proteins implicated in the proliferation of human hair follicle dermal papilla (hHFDP) cells. In this human trial, using a phototrichogram, the effect of B. papyrifera on hair growth was examined by reconstitution analysis after shaving the hair of the clinical subject’s dorsal skin. B. papyrifera promoted growth equally in hHFDP cells, which is comparable to that of minoxidil and tofacitinib. Treatment with B. papyrifera extract enhanced the TCF/LEF-luciferase activity and increased the level of β-catenin protein. Moreover, B. papyrifera extract significantly suppressed interleukin-4 (IL4)-induced STAT6 phosphorylation. In clinical trial, using a phototrichogram, we assessed the hair density and total hair counts at 0, 6, and 12 weeks after the use of hair tonic containing B. papyrifera extract. After using the hair tonic for 12 weeks, the total hair count was significantly increased as compared with the subjects at the start date (n = 11). B. papyrifera promotes dermal papilla cells proliferation in vitro and clinically among human volunteers through the regulation of WNT-β-catenin and STAT6 pathways.

scholarly journals In Vitro Hair Dermal Papilla Cells Induction by Fagraea berteroana, a Tree of the Marquesan Cosmetopoeia (French Polynesia)

Cosmetics ◽  
2021 ◽  
Vol 8 (1) ◽  
pp. 13
Author(s):  
Kristelle Hughes ◽  
Raimana Ho ◽  
Claire Chazaud ◽  
Stéphanie Hermitte ◽  
Stéphane Greff ◽  
...  
Keyword(s):  
Transforming Growth Factor Beta ◽  
Hair Growth ◽  
Transforming Growth Factor ◽  
Dermal Papilla ◽  
Fold Increase ◽  
French Polynesia ◽  
Bone Morphogenetic Protein 2 ◽  
Dermal Papilla Cells ◽  
Growth State

Fagraea berteroana is a tree used in traditional medicine in various islands of the South Pacific. Here, we studied its hair growth-inducing properties as suggested by one of its Marquesan ethno-uses in haircare. The ethyl acetate extract of the fruits of F. berteroana (FEAE) and four resulting fractions (FEAE-F0, FEAE-F1, FEAE-F2, and FEAE-F3) were tested on hair follicle dermal papilla cells to determine their cell proliferative activity. Furthermore, RT-qPCR analysis enabled gene modulation analysis, while immunostaining of the β-catenin protein was used to follow protein regulation. We found that the plant extracts induced a controlled, dose-dependent cell proliferation. FEAE-F0 simultaneously down-regulated Bone Morphogenetic Protein 2 (BMP2) mRNA expression and upregulated Cyclin-D1 (CCND1) gene expression, which suggests an involvement in the regulation of the Wnt and Transforming Growth Factor beta (TGFβ) pathways that control the hair cycle. FEAE-F0 exhibited a 1.34-fold increase of nuclear β-catenin protein. This is indicative of an active hair growth state. Thus, we conclude that FEAE-F0 could be an innovative candidate in hair care, which opens interesting leads to promote the Marquesan cosmetopoeia.

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