Impact of Superovulation And In Vitro Fertilization On LINE-1 Copy Number And Telomere Length In C57BL/6J Mice Blastocysts

Abstract Objective: Millions of babies have been conceived by IVF, yet debate about its safety to offspring continues. We hypothesized that superovulation and in vitro fertilization (IVF) promote genomic changes, including altered telomere length (TL) and activation of the retrotransposon LINE-1 (L1), and tested this hypothesis in a mouse model. Material and methods: Experimental study analyzing TL and L1 copy number in C57BL/6J mouse blastocysts in vivo produced from natural mating cycles (N), in vivo produced following superovulation (S), or in vitro produced following superovulation (IVF). We also examined the effects of prolonged culture on TL and L1 copy number in the IVF group comparing blastocysts cultured 96 hours versus blastocysts cultured 120 hours. TL and L1 copy number were measured by Real Time PCR. Results: TL in S (n=77; Mean: 1.50± 1.15; p=0.0007) and IVF (n=82; Mean: 1.72± 1.44; p<0.0001) exceeded that in N (n=16; Mean: 0.61± 0.27). TL of blastocysts cultured 120 hours (n=15, Mean: 2.14± 1.05) was significantly longer than that of embryos cultured for 96 hours (n=67, Mean: 1.63 ± 1.50; p=0.0414). L1 copy number of blastocysts cultured for 120 hours (n=15, Mean: 1.71± 1.49) exceeded that of embryos cultured for 96 hours (n=67, Mean: 0.95 ± 1.03; p=0.0162). Conclusions: Intriguingly ovarian stimulation, alone or followed by IVF, produced embryos with significantly longer telomeres compared to in vivo, natural cycle-produced embryos. The significance of this enriched telomere endowment for the health and longevity of offspring born from IVF merit future studies.

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IMPACT OF SUPEROVULATION AND IN VITRO FERTILIZATION ON LINE 1 COPY NUMBER AND TELOMERE LENGTH IN C57BL/6J MICE BLASTOCYSTS

Fertility and Sterility ◽

10.1016/j.fertnstert.2021.07.465 ◽

2021 ◽

Vol 116 (3) ◽

pp. e168-e169

Author(s):

Thalita S. Berteli ◽

Fang Wang ◽

Fabiana B. Kohlrausch ◽

Caroline M Da Luz ◽

Fernanda V. Oliveira ◽

...

Keyword(s):

In Vitro Fertilization ◽

Telomere Length ◽

Copy Number ◽

Vitro Fertilization ◽

On Line

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Prevalence of pathogenic copy number variants among children conceived by donor oocyte

Scientific Reports ◽

10.1038/s41598-021-86242-x ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Sandra Monfort ◽

Carmen Orellana ◽

Silvestre Oltra ◽

Mónica Rosello ◽

Alfonso Caro-Llopis ◽

...

Keyword(s):

In Vitro Fertilization ◽

Copy Number ◽

Assisted Reproductive Technologies ◽

Copy Number Variants ◽

Reproductive Technologies ◽

Significant Excess ◽

Vitro Fertilization ◽

Donor Oocyte ◽

Increased Risk

AbstractDevelopment of assisted reproductive technologies to address infertility has favored the birth of many children in the last years. The majority of children born with these treatments are healthy, but some concerns remain on the safety of these medical procedures. We have retrospectively analyzed both the fertilization method and the microarray results in all those children born between 2010 and 2019 with multiple congenital anomalies, developmental delay and/or autistic spectrum disorder (n = 486) referred for array study in our center. This analysis showed a significant excess of pathogenic copy number variants among those patients conceived after in vitro fertilization with donor oocyte with respect to those patients conceived by natural fertilization (p = 0.0001). On the other hand, no significant excess of pathogenic copy number variants was observed among patients born by autologous oocyte in vitro fertilization. Further studies are necessary to confirm these results and in order to identify the factors that may contribute to an increased risk of genomic rearrangements, as well as consider the screening for genomic alterations after oocyte donation in prenatal diagnosis.

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In Vitro Fertilization of In Vitro and In Vivo Matured Oocytes from Prepubertal Meishan Gilts

Nihon Chikusan Gakkaiho ◽

10.2508/chikusan.61.1011 ◽

1990 ◽

Vol 61 (11) ◽

pp. 1011-1016

Author(s):

Takashi MIYANO ◽

Kiyoshi YOSHIKAWA ◽

Seishiro KATO ◽

Hiroshi HARAYAMA ◽

Iwao NANJO ◽

...

Keyword(s):

In Vitro Fertilization ◽

Vitro Fertilization

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Piglets produced by transfer of embryos obtained by in vitro fertilization of oocytes matured in vitro with thymosin: A case report

Medycyna Weterynaryjna ◽

10.21521/mw.6356 ◽

2020 ◽

Vol 76 (03) ◽

pp. 6356-2020 ◽

Cited By ~ 2

Author(s):

KATARZYNA PONIEDZIAŁEK-KEMPNY ◽

BARBARA GAJDA ◽

IWONA RAJSKA ◽

LECHOSŁAW GAJDA ◽

ZDZISŁAW SMORĄG

Keyword(s):

Case Report ◽

In Vitro Fertilization ◽

Control Group ◽

First Birth ◽

Vitro Fertilization ◽

Research Material ◽

Preliminary Study ◽

Experimental Group

The aim of the study was to examine the in vivo viability of in vitro-produced (IVP) porcine embryos obtained from oocytes matured with thymosin. The research material for this study consisted of immature pig oocytes obtained from ovaries after slaughter and ejaculated semen obtained from one boar. The immature oocytes were cultured in vitro until the metaphase II stage in a medium supplemented with thymosin (TMS). The presumptive zygotes obtained were cultured in vitro for 4-40 hours. The presumptive zygotes and 2-4-cell embryos were evaluated in vivo after transferring them to synchronized recipients. After the transfer of embryos from the experimental group into 2 recipients (50 embryos into each gilt) and the transfer of 50 embryos from the control group into 1 recipient, both gilts that had received embryos obtained by in vitro fertilization of oocytes matured with TMS became pregnant and delivered a total of 16 live piglets. After the transfer of embryos from the control group, no pregnancy was achieved. In conclusion, the results of our preliminary study suggest that the maturation of pig oocytes with thymosin supports the in vivo survival of in vitro produced embryos. It is important to note, that this was the first birth of piglets obtained after transfer of IVP embryos in Poland.

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