The Effect of the mGlu8 Receptor Agonist, S-3,4-DCPG on Acquisition and Expression of Morphine-induced Conditioned Place Preference in Male rats

Abstract Background: The nucleus accumbens (NAc) plays a principal role in drug reward. It has been reported that metabotropic glutamate receptors (mGlu receptors) play a key role in the rewarding pathway(s). Previous studies have shown the vast allocation of the different types of mGlu receptors, including mGlu8 receptors, in regions that are associated with opioid rewards, such as the NAc. The aim of the present study was to evaluate the role of mGlu8 receptors within the NAc in the acquisition and expression phases of morphine induced conditioned place preference (CPP). Adult male Wistar rats were bilaterally implanted by two cannulas' in the NAc and were evaluated in a CPP paradigm. Selective mGlu8 receptor allosteric agonist (S-3,4-DCPG) was administered at doses of 0.03, 0.3, and 3 μg/0.5 μL saline per side into the NAc on both sides during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase, or before place preference test, or post-conditioning (expression) phase of morphine-induced CPP. Results: The results revealed that intra-accumbal administration of S-3,4-DCPG (0.3 and 3 μg) markedly decreased the acquisition in a dose-dependent manner but had no effect on expression of morphine-induced CPP. Conclusions: The findings suggest that activation of mGlu8 receptors in the NAc dose-dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine which may be related to the glutamate activity into the NAc and in reward pathway(s). These data suggest that mGlu8 receptor may be involved in conditioned morphine reward.

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The Effect of the mGluR8 Agonist, S-3,4-DCPG on Acquisition and Expression of Morphine-Induced Conditioned Place Preference in Male Rats

10.21203/rs.3.rs-114995/v1 ◽

2020 ◽

Author(s):

Nazanin Kahvandi ◽

Zahra Ebrahimi ◽

Seyed Asaad Karimi ◽

Siamak Shahidi ◽

Iraj Salehi ◽

...

Keyword(s):

Conditioned Place Preference ◽

Metabotropic Glutamate Receptors ◽

Preference Test ◽

Place Preference ◽

Male Rats ◽

Dependent Manner ◽

Metabotropic Glutamate ◽

Male Wistar Rats ◽

Dose Dependent

Abstract BackgroundThe nucleus accumbens (NAc) plays a principal role in drug reward. It has been reported that metabotropic glutamate receptors (mGluRs) play a key role in the rewarding pathway(s). Previous studies have shown the vast allocation of the different types of mGluRs, including mGluR8, in regions that are associated with opioid rewards, such as the NAc. The aim of the present study was to evaluate the role of mGlu8 receptors within the NAc in the acquisition and expression phases of morphine induced conditioned place preference (CPP). Adult male Wistar rats were bilaterally implanted by two cannulas' in the NAc and were evaluated in a CPP paradigm. Selective mGluR8 allosteric agonist (S-3,4-DCPG) was administered at doses of 0.03, 0.3, and 3μg/0.5 μL saline per side into the NAc on both sides during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase, or before place preference test, or post-conditioning (expression) phase of morphine-induced CPP. ResultsThe results revealed that intra-accumbal administration of S-3,4-DCPG (0.3 and 3 μg) markedly decreased the acquisition in a dose-dependent manner but had no effect on expression of morphine-induced CPP. ConclusionsThe findings suggest that activation of mGlu8 receptors in the NAc dose-dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine which may be related to the glutamate activity into the NAc and/or synaptic plasticity of this system in reward pathway(s).

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The effect of the mGlu8 receptor agonist, (S)-3,4-DCPG on acquisition and expression of morphine-induced conditioned place preference in male rats

Behavioral and Brain Functions ◽

10.1186/s12993-021-00174-0 ◽

2021 ◽

Vol 17 (1) ◽

Author(s):

Nazanin Kahvandi ◽

Zahra Ebrahimi ◽

Seyed Asaad Karimi ◽

Siamak Shahidi ◽

Iraj Salehi ◽

...

Keyword(s):

Conditioned Place Preference ◽

Metabotropic Glutamate Receptors ◽

Preference Test ◽

Place Preference ◽

Male Rats ◽

Dependent Manner ◽

Metabotropic Glutamate ◽

Male Wistar Rats ◽

Dose Dependent

Abstract Background The nucleus accumbens (NAc) plays a principal role in drug reward. It has been reported that metabotropic glutamate receptors (mGlu receptors) play a key role in the rewarding pathway(s). Previous studies have shown the vast allocation of the different types of mGlu receptors, including mGlu8 receptors, in regions that are associated with opioid rewards, such as the NAc. The aim of the present study was to evaluate the role of mGlu8 receptors within the NAc in the acquisition and expression phases of morphine induced conditioned place preference (CPP). Adult male Wistar rats were bilaterally implanted by two cannulas' in the NAc and were evaluated in a CPP paradigm. Selective mGlu8 receptor allosteric agonist (S-3,4-DCPG) was administered at doses of 0.03, 0.3, and 3 μg/0.5 μL saline per side into the NAc on both sides during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase, or before place preference test, or post-conditioning (expression) phase of morphine-induced CPP. Results The results revealed that intra-accumbal administration of S-3,4-DCPG (0.3 and 3 μg) markedly decreased the acquisition in a dose-dependent manner but had no effect on expression of morphine-induced CPP. Conclusions The findings suggest that activation of mGlu8 receptors in the NAc dose-dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine which may be related to the glutamate activity into the NAc and in reward pathway(s). These data suggest that mGlu8 receptor may be involved in conditioned morphine reward.

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The Role of mGluR4 Receptors Within the Nucleus Accumbens in the Acquisition and Expression of Morphine-induced Conditioned Place Preference in Male Rats

10.21203/rs.3.rs-147897/v1 ◽

2021 ◽

Author(s):

Zahra Ebrahimi ◽

Nazanin Kahvandi ◽

Alireza Komaki ◽

Seyed Asaad Karimi ◽

Marzieh Naderishahab ◽

...

Keyword(s):

Nucleus Accumbens ◽

Metabotropic Glutamate Receptors ◽

Place Preference ◽

Male Rats ◽

Dependent Manner ◽

Metabotropic Glutamate ◽

Male Wistar Rats ◽

Glutamate Neurotransmission ◽

Reward Pathways

Abstract Background: Several studies have shown that glutamate neurotransmission in the nucleus accumbens (NAc) is required for the development of morphine-induced conditional place preference (CPP). Also, metabotropic glutamate receptors (mGluRs) into the NAc play important roles in the reward pathways. However, the precise role of mGluR4 in different steps of the morphine-induced CPP is less well known. In the present study we investigated the effect of bilateral intra-accumbal infusion of VU0155041, as a specific mGluR4 agonist on the acquisition and expression of morphine induced CPP in male Wistar rats. Animals were bilaterally implanted with guide cannulae above the NAc. In the first part of the study, the VU0155041 was administered at doses of 10, 30 and 50 μg/0.5 μL saline per side into the NAc during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase of morphine-induced CPP. In the next part of the study, the rats bilaterally received VU0155041 at the dose of 50 μg/0.5 μL, 5 min before the post-conditioning test to check the effect of VU0155041 on the expression of morphine-induced CPP. Results: The results showed that the intra-accumbal injection of VU0155041 inhibits the acquisition of morphine-induced CPP in a dose dependent manner, but had no effect on expression Our data indicated that intra-NAc administration of VU0155041 dose-dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine.These effects may be related to changes in glutamate activity in the NAC and/or learning dependent mechanism of glutamate neurotransmission in reward pathway(s).

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The role of mGlu4 receptors within the nucleus accumbens in acquisition and expression of morphine-induced conditioned place preference in male rats

BMC Neuroscience ◽

10.1186/s12868-021-00627-2 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Zahra Ebrahimi ◽

Nazanin Kahvandi ◽

Alireza Komaki ◽

Seyed Asaad Karimi ◽

Marzieh Naderishahab ◽

...

Keyword(s):

Nucleus Accumbens ◽

Metabotropic Glutamate Receptors ◽

Place Preference ◽

Male Rats ◽

Dependent Manner ◽

Metabotropic Glutamate ◽

Male Wistar Rats ◽

Glutamate Neurotransmission ◽

Reward Pathways

Abstract Background Several studies have shown that glutamate neurotransmission in the nucleus accumbens (NAc) is required for the development of morphine-induced conditional place preference (CPP). In addition, metabotropic glutamate receptors (mGluRs) in NAc play important roles in the reward pathways. However, the precise role of mGluR4 in different steps of the morphine-induced CPP is less well known. In the present study the effect of bilateral intra-accumbal infusion of VU0155041, as a specific mGluR4 agonist on the acquisition and expression of morphine induced CPP in male Wistar rats was investigated. The animals were bilaterally implanted with guide cannulae above the NAc. In the first step of the study, the VU0155041 was administered at doses of 10, 30 and 50 μg/0.5 μL saline per side into the NAc during the 3 days of morphine (5 mg/kg) conditioning (acquisition) phase of morphine-induced CPP. In the second step of the study, the rats bilaterally received VU0155041 at the dose of 50 μg/0.5 μL, 5 min before the post-conditioning test in order to check the effect of VU0155041 on the expression of morphine-induced CPP. Results The results showed that the intra-accumbal injection of VU0155041 inhibits the acquisition of morphine-induced CPP in a dose dependent manner, but had no effect on expression. Conclusions The data indicated that intra-NAc administration of VU0155041 dose dependently blocks the establishment of morphine-induced CPP and reduces the rewarding properties of morphine. These effects may be related to changes in glutamate activity in the NAC and/or learning dependent mechanism of glutamate neurotransmission in reward pathway(s).

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Conditioned place preference of kisspeptin-10

Reviews on Clinical Pharmacology and Drug Therapy ◽

10.17816/rcf19147-53 ◽

2021 ◽

Vol 19 (1) ◽

pp. 47-53

Author(s):

Ilia Yu. Tissen ◽

Polina A. Chepik ◽

Andrei A. Lebedev ◽

Leila A. Magarramova ◽

Eugenii R. Bychkov ◽

...

Keyword(s):

Conditioned Place Preference ◽

Open Field Test ◽

Physiological Saline ◽

Preference Test ◽

Brain Regions ◽

Place Preference ◽

Male Rats ◽

Plus Maze ◽

Male Wistar Rats ◽

Positive State

INTRODUCTION: Kisspeptins (KISS), a group of brain neuropeptides are involved in sexual behavior. KISS activate the hypothalamic neurons that synthesize gonadotropin releasing hormone. KISS was also detected in the limbic system. Earlier, we showed the activation of sexual motivation after the administration of kisspeptin-10 without increasing the level of testosterone in male rats, which suggests the extrahypothalamic effect of KISS. The aim of this work was to study the possibility of aquisition of conditioned place preference of kisspeptin-10, as well as to study the emotional and investigational behavior in rats after intranasal peptide administration. METHODS: Conditioned place preference test (CPP), open field test (OP) and elevated plus maze (EPM) were used in male Wistar rats. RESULTS: When studying CPP, animals spent 78.6 6.3% of the time in the chamber associated with the administration of KISS compared to control animals with administration of physiological saline (51.2% of the experiment time; p 0.05). After kisspeptin-10 administration locomotor activity was 2-fold increased (p 0.05), and the number of sniffings was 2-fold increased too (p 0.05). The parameters did not significantly differ in animals treated with kisspeptin or saline in PCL. CONCLUSION: Thus repeated intranasal administration of kisspeptin-10 induces the aquisition of CPP in rats. This suggests that kisspeptin-10 can cause activity in the reward system or the activation of brain regions associated with this system, which ultimately leads to the formation of an emotionally positive state.

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Triadimefon triggers circling behavior and conditioned place preference/aversion in zebrafish in a dose dependent manner

Neurotoxicology and Teratology ◽

10.1016/j.ntt.2021.106979 ◽

2021 ◽

pp. 106979

Author(s):

Susana Paredes-Zúñiga ◽

Fernando Ormeño ◽

Miguel L. Allende

Keyword(s):

Conditioned Place Preference ◽

Place Preference ◽

Dependent Manner ◽

Circling Behavior ◽

Dose Dependent

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Role of neurotensin injected into the ventral pallidum in open field and in conditioned place preference test.

Frontiers in Neuroscience ◽

10.3389/conf.fnins.2011.84.00010 ◽

2011 ◽

Vol 5 ◽

Author(s):

Lénárd L.

Keyword(s):

Conditioned Place Preference ◽

Open Field ◽

Preference Test ◽

Place Preference ◽

Ventral Pallidum ◽

Conditioned Place Preference Test

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Role of metabotropic glutamate receptors in vasopressin and oxytocin release

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.2001.281.2.r452 ◽

2001 ◽

Vol 281 (2) ◽

pp. R452-R458 ◽

Cited By ~ 16

Author(s):

Delmore J. Morsette ◽

Hanna Sidorowicz ◽

Celia D. Sladek

Keyword(s):

Metabotropic Glutamate Receptors ◽

Metabotropic Glutamate Receptor ◽

Dependent Manner ◽

Hormone Release ◽

Concentration Dependent Manner ◽

Group Iii ◽

Metabotropic Glutamate ◽

Group I ◽

Oxytocin Release

The effect of metabotropic glutamate receptor (mGluR) activation on vasopressin (VP) and oxytocin (OT) release was evaluated using explants of the hypothalamoneurohypophysial system. (+/−)-1-Aminocyclopentane- trans-1,3-dicarboxylic acid (t-ACPD), an agonist at groups I and II mGluRs, increased VP and OT release in a concentration-dependent manner. A role for group I mGluRs in VP and OT release was demonstrated by the ability of a group I-specific mGluR antagonist, 1-aminoindan-1,5-idicarboxylic acid (AIDA), to block the effect of t-ACPD and the ability of a group I-specific agonist, ( R, S)-3,5-dihydroxyphenylglycine, to significantly increase both VP ( P = 0.0029) and OT ( P = 0.0032) release. However, AIDA did not alter VP or OT release induced by a ramp increase in osmolality of the perifusion medium. The role of group III mGluRs was examined using L(+)-2-amino-4-phosphonobutyric acid (L-AP4), an agonist of these receptors. L-AP4 did not change basal release of VP or OT and did not prevent osmotically stimulated hormone release. Thus mGluR activation stimulates VP and OT release, but it is not required for osmotic stimulation of hormone release.

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