scholarly journals A broad-range survey of Borrelia burgdorferi sensu lato infection in small mammals in Yunnan Province, China

2020 ◽  
Author(s):  
Zhihai He ◽  
Baogui Jiang ◽  
Zihou Gao ◽  
Zongti Shao ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Yunnan Province ◽  
23S Rrna ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Tropical Region ◽  
Prevalence Rates ◽  
High Genetic Diversity ◽  
Landscape Types ◽  
Pcr Targeting

Abstract Background: Lyme disease, caused by Borrelia burgdorferi sensu lato (BBSL), is commonly found in wild and domestic mammals worldwide. In China, human cases of B. burgdorferi infections have been identified across a wide distribution, but little direct surveillance of potential small mammal reservoirs has been performed in Yunnan Province, a tropical region in southwestern China. Here we report a thorough investigation of BBSL in small mammals collected from 2011 to 2016 from this region.Methods: Small mammals were captured using snap traps in 23 counties located in Yunnan Province. DNA was extracted from spleen tissue using DNA blood and tissue kits. A nested PCR targeting the 5S-23S rRNA intergenic spacer gene of BBSL was used for pathogen detection. Amplicons of 252bp expected sizes were sequenced directly and analyzed using BLAST algorithm. A phylogenetic tree was constructed using MEGA software and statistical analysis were conducted using SPSS version 17.0.Results: Overall, 3659 mammals belonging to 57 species were captured at 159 sample sites located in 23 counties in Yunnan Province. Borrelia burgdorferi s.l. was found in 146 mammals (3.99%), from 30 different species, 20 of which represent the first reported detection in that species. Sequence analysis revealed five genotypes of B. burgdorferi s.l., including B. afzelii, B. burgdorferi sensu stricto, B. japonica, B. garinii and B. valaisiana.Conclusions: Significant differences in prevalence rates of BBSL were observed at varying landscape types and altitudes. Small mammals in forested areas had higher prevalence rates than other landscape types, as did small mammals found at altitudes greater than 2500 meters. The five genotypes of BBSL detected, suggests high genetic diversity within this province.

A broad-range survey of Borrelia burgdorferi sensu lato infection in small mammals in Sino-Burmese border area, Yunnan Province, China

2020 ◽  
Author(s):  
Zhihai He ◽  
Baogui Jiang ◽  
Zihou Gao ◽  
Zongti Shao ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Nested Pcr ◽  
Yunnan Province ◽  
Intergenic Spacer ◽  
23S Rrna ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Prevalence Rates ◽  
Landscape Types

Abstract Background: Lyme disease is caused by Borrelia burgdorferi sensu lato which is usually found in wild and domestic mammals worldwide. In China, Human cases of B. burgdorferi infections have been identified in a wide distribution, but little direct surveillance of potential rodent reservoirs has been performed in Yunnan Province, the tropical region, Sino-Burmese border area, southwestern China. Here we report a thoroughly investigation of B. burgdorferi sensu lato in small mammals collected in 2011 to 2016 in the region.Methods: A nested PCR was done for the 5S-23S rRNA intergenic spacer gene of Borrelia burgdorferi sensu lato. The PCR-positive amplicons were directly sequenced. Sequence analysis was carried out using a FASTA search on the Genbank database, with phylogenetic trees constructed using MEGA software, version 6.06. Statistical Analysis were conducted using SPSS.version 17.0.Results: Totally 57 species, 3659 mammals were captured at 159 sample sites located in 23 counties in Yunnan Province. Thirty species, 146 (3.99%) mammals were tested positive for B. bourgdorferi s.l . by nested PCR based on 5S-23S rRNA intergenic spacer gene. 20 species mammals were first reported infected with B. burgdorferi s.l. Sequence analysis revealed that five genotypes of B. burgdorferi s.l. were detected, including B. afzelii , B. burgdorferi sensu stricto, B. japonica , B. garinii and B. valaisiana.Conclusions: Significant differences in prevalence rates of B. bourgdorferi s.l were observed at varying landscape types and altitudes. Small mammals in forested areas had higher prevalence rates than other landscape types as did small mammals found at altitudes greater than 2500 meters. The 5S-23S rRNA intergenic spacer gene revealed that there were five genotypes of B. burgdorferi s.l. detected, indicating high genetic diversity within this province.

scholarly journals Infection of Borrelia burgdorferi sensu lato of small mammals in Yunnan Province, China

2019 ◽  
Author(s):  
Zhi-hai He ◽  
Bao-gui Jiang ◽  
Zi-hou Gao ◽  
Zong-ti Shao ◽  
Yun Zhang ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Yunnan Province ◽  
Wide Distribution ◽  
Southwestern China ◽  
23S Rrna ◽  
Prevalence Rates ◽  
Landscape Types

AbstractBackgroundLyme disease is caused by Borrelia burgdorferisensulato (BBSL) which is usually found in wild and domestic mammals worldwide. Human cases of B. burgdorferi infections have been identified in China, but little direct surveillance of potential rodent reservoirs has been performed in Yunnan Province, Southwestern China. Yunnan Province is a tropical area with a diverse topographic range and sustains a high biodiversity of small mammals that could potentially play an important role in the transmission of a variety of B. burgdorferigenospecies.Methods3659 small mammals were captured in 159 sample siteslocated 23 countries inYunnan Province and screened for BBSL infection by nested PCR based on 5S-23S rRNA intergenic spacer gene of BBSL.Univariate and multivariate forward stepwise logistic regression analysis was used to access the association between infections and related risk factors.ResultsInfection with BBSL was confirmed in 3.99%(146/3659) of small mammals. Significant differences in prevalence rates of BBSL were observed at varying landscape types and altitudes.Small mammals in forested areas had higher prevalence rates than other landscape types as did small mammals found at altitudes greater than 2500 meters. The 5S-23S rRNA intergenic spacergene revealed that there were 5 genotype of BBSL, including B. afzelii, B. burgdorferisensustricto, B.japonica, B.gariniiand B.valaisiana, which demonstrate the genetic diversity and regional distribution.ConclusionsThere exists a wide distribution and genetic diversity of endemic BBSL in Southwestern China, warranting further investigations and monitoring of clinical disease in individuals presenting with symptoms of Lyme disease in these areas.Author summaryLyme disease is caused by Borrelia burgdorferi sensu lato (BBSL) which is usually found in wild and domestic mammals worldwide. Human cases of Borrelia burgdorferi sensu lato infections have been identified in China, but little direct surveillance of potential rodent reservoirs has been performed in Southwestern China. This study documents potential small mammal reservoir hosts collected from a large of sample sites from different landscape types and altitudes, with PCR and sequencing identifying the wide distribution and genetic diversity of endemic Borrelia burgdorferi sensu lato in Southwestern China. This was the first report that B. japonica was detected in Apodemus draco and Niviventer excelsior in China. This study adds to body of literature on Borrelia burgdorferi sensu lato in China. This work will provide insight regarding small mammals to target for surveillance and we access the association between gender, developmental stage of rodents, environmental landscape and altitude to better prevent human exposure.

scholarly journals Differential Transmission of the Genospecies of Borrelia burgdorferi Sensu Lato by Game Birds and Small Rodents in England

1998 ◽  
Vol 64 (4) ◽  
pp. 1169-1174 ◽  
Author(s):  
Klaus Kurtenbach ◽  
Mick Peacey ◽  
Sjoerd G. T. Rijpkema ◽  
Andrew N. Hoodless ◽  
Patricia A. Nuttall ◽  
...  
Keyword(s):  
Borrelia Burgdorferi ◽  
Lyme Borreliosis ◽  
Sensu Stricto ◽  
Rrna Genes ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Internal Organs ◽  
Borrelia Garinii ◽  
Game Birds ◽  
5S Rrna Genes ◽  
Pcr Targeting

ABSTRACT The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In questingIxodes ricinus ticks, three genospecies of B. burgdorferi sensu lato were detected, with the highest prevalences found for Borrelia garinii and Borrelia valaisiana. B. burgdorferi sensu stricto was rare (<1%) in all tick stages. Borrelia afzelii was not detected in any of the samples. More than 50% of engorged nymphs collected from pheasants were infected with borreliae, mainly B. garinii and/orB. valaisiana. Although 19% of the rodents harboredB. burgdorferi sensu stricto and/or B. gariniiin internal organs, only B. burgdorferi sensu stricto was transmitted to xenodiagnostic tick larvae (it was transmitted to 1% of the larvae). The data indicate that different genospecies of B. burgdorferi sensu lato can be maintained in nature by distinct transmission cycles involving the same vector tick species but different vertebrate host species. Wildlife management may have an influence on the relative risk of different clinical forms of Lyme borreliosis.

scholarly journals Geographical Distribution of Borrelia burgdorferi sensu lato in Ticks Collected from Wild Rodents in the Republic of Korea

Pathogens ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 866
Author(s):  
Seong Yoon Kim ◽  
Tae-Kyu Kim ◽  
Tae Yun Kim ◽  
Hee Il Lee
Keyword(s):  
Sequence Analysis ◽  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Jeju Island ◽  
Republic Of Korea ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Wild Rodents ◽  
The Republic ◽  
Tick Vectors ◽  
Pcr Targeting

Lyme disease is a tick-borne zoonotic disease caused by Borrelia burgdorferi sensu lato (s. l.) via transmission cycles involving competent tick vectors and vertebrate reservoirs. Here, we determined the prevalence and distribution of Borrelia genospecies in 738 ticks of at least three species from wild rodents in nine regions of the Republic of Korea (ROK). Ticks were analyzed using nested PCR targeting partial flagellin B gene sequences, followed by sequence analysis. The prevalence of Borrelia infection was 33.6%, and the most common genospecies were B. afzelii (62.5%), B. valaisiana (31.9%), B. yangtzensis (2.4%), B. garinii (1.6%), and B. tanukii (1.6%). Borrelia afzelii was found in all regions except Jeju Island; this predominant genospecies was found in the northern and central sampling regions. Borrelia valaisiana, B. yangtzensis, and B. tanukii were found only in the southern regions with B. valaisiana being the most common, whereas B. yangtzensis and B. tanukii were only found on Jeju Island. Our study is the first to describe the nationwide prevalence of B. burgdorferi s. l. in ticks from wild rodents in the ROK. Continuous surveillance in ticks, animals, humans, and different regions is required to avoid disease distribution and possible transmission to humans in the ROK.

scholarly journals LYME BORRELIOSIS IN NORTH BACKA DISTRICT

2017 ◽  
Vol 10 (2) ◽  
pp. 81-94
Author(s):  
Dragica Kovačević Berić ◽  
Tibor Molnar ◽  
Morana Miković ◽  
Sara Savić
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Average Rate ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Prevalence Rates ◽  
Serological Tests ◽  
Causative Agents ◽  
Vector Borne ◽  
Epidemiological Characteristics ◽  
Moderate Climate

Lyme disease is the most common vector borne disease in regions with moderate climate. The cause of the disease is Borrelia burgdorferi sensu lato (B. burgdorferi s.l.), whereas infection is spread via bite from a tick carrying the causative agent. The objective of this work is the examination of descriptive-epidemiological characteristics of Lyme disease in North Backa region in the period from 2012 to 2016. In terms of classifi cation, Lyme dis-ease does not fall into the category of diseases that must be reported when infection occurs among animals. The average rate of incidence in humans during the observed five-year period is 7/100.000. Th e highest incidence of this disease among humans was observed in 2013 (In 12.2/100,000) with 22 reported cases, whereas the lowest incidence was in 2015 (In 5.0/100,000) with 9 reported cases. The highest rate of incidence was recorded in Bačka Topola municipality – 15.9/100.000; followed by Mali Iđoš with 7.2/100.000; while the lowest rate was recorded in Subotica being 5.0 cases per 100,000 residents. Th e presence of the disease was confi rmed in all patients via labo-ratory (serological) tests. Women were more likely to become infected, with the male-female patient ratio being 41%-59%. The disease was documented predominantly among people aged 50-69. The majority of cases were re-corded during spring and summer, in the period from May to June (71%). Among animals, the disease was documented in Subotica and Mali Iđoš municipality, with average prevalence rates among dogs and horses being 0.07%; and 1.52% respectively. Subotica municipality has the most accurate records of cases and a prevalence of 0.06% among dogs, and 1.43% among horses, while Mali Iđoš municipality has higher prevalence, being 2.99% among horses and 0.14% among dogs. The highest occurrence of Lyme disease among animals was documented in 2015, with a prevalence of 2.85% among horses. The presence of causative agents among examined ticks in Mali Iđoš municipality was 16.28% on average, predominantly in 2015 (in 21.21% of examined ticks).

scholarly journals Circulation of Tick-Borne Spirochetes in Tick and Small Mammal Communities in Santa Barbara County, California, USA

2020 ◽  
Vol 57 (4) ◽  
pp. 1293-1300 ◽  
Author(s):  
Andrew J MacDonald ◽  
Sara B Weinstein ◽  
Kerry E O’Connor ◽  
Andrea Swei
Keyword(s):  
Lyme Disease ◽  
Borrelia Burgdorferi ◽  
Small Mammals ◽  
Small Mammal ◽  
Southern California ◽  
Preliminary Evidence ◽  
Transmission Risk ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Disease Agent ◽  
Lyme Disease Agent

Abstract A diversity of Borrelia burgdorferi sensu lato (Johnson, Schmid, Hyde, Steigerwalt & Brenner) (Spirochaetales: Spirochaetaceae) genomospecies, including the Lyme disease agent, Borrelia burgdorferi sensu stricto (s.s.), have been identified in the western United States. However, enzootic transmission of B. burgdorferi s.l. in small mammals and ticks is poorly characterized throughout much of the region. Here we report prevalence of B. burgdorferi s.l. in small mammal and tick communities in the understudied region of southern California. We found B. burgdorferi s.l. in 1.5% of Ixodes species ticks and 3.6% of small mammals. Infection was uncommon (~0.3%) in Ixodes pacificus Cooley and Kohls (Acari: Ixodidae), the primary vector of the Lyme disease agent to humans in western North America, but a diversity of spirochetes—including Borrelia bissettiae, Borrelia californiensis, Borrelia americana, and B. burgdorferi s.s.—were identified circulating in Ixodes species ticks and their small mammal hosts. Infection with B. burgdorferi s.l. is more common in coastal habitats, where a greater diversity of Ixodes species ticks are found feeding on small mammal hosts (four species when compared with only I. pacificus in other sampled habitats). This provides some preliminary evidence that in southern California, wetter coastal areas might be more favorable for enzootic transmission than hotter and drier climates. Infection patterns confirm that human transmission risk of B. burgdorferi s.s. is low in this region. However, given evidence for local maintenance of B. burgdorferi s.l., more studies of enzootic transmission may be warranted, particularly in understudied regions where the tick vector of B. burgdorferi s.s. occurs.

scholarly journals Antibodies Related to Borrelia burgdorferi sensu lato, Coxiella burnetii, and Francisella tularensis Detected in Serum and Heart Rinses of Wild Small Mammals in the Czech Republic

Pathogens ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 419
Author(s):  
Alena Žákovská ◽  
Eva Bártová ◽  
Pavlína Pittermannová ◽  
Marie Budíková
Keyword(s):  
Czech Republic ◽  
Borrelia Burgdorferi ◽  
Francisella Tularensis ◽  
Coxiella Burnetii ◽  
Small Mammals ◽  
Zoonotic Diseases ◽  
Myodes Glareolus ◽  
Borrelia Burgdorferi Sensu Lato ◽  
Enzyme Linked Immunosorbent Assay ◽  
The Czech Republic

Wild small mammals are the most common reservoirs of pathogenic microorganisms that can cause zoonotic diseases. The aim of the study was to detect antibodies related to Borrelia burgdorferi sensu lato, Coxiella burnetii, and Francisella tularensis in wild small mammals from the Czech Republic. In total, sera or heart rinses of 211 wild small mammals (168 Apodemus flavicollis, 28 Myodes glareolus, 9 A. sylvaticus, and 6 Sorex araneus) were examined by modified enzyme-linked immunosorbent assay. Antibodies related to B.burgdorferi s.l., C. burnetii, and F. tularensis were detected in 15%, 19%, and 20% of animals, respectively. The prevalence of B. burgdorferi and F. tularensis statistically differed in localities and F. tularensis also differed in sex. Antibodies against 2–3 pathogens were found in 17% of animals with a higher prevalence in M. glareolus. This study brings new data about the prevalence of the above-mentioned pathogens.

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