scholarly journals Significance of Cytoplasmic Expression of Telomerase Reverse Transcriptase in Patients with Hepatocellular Carcinoma Undergoing Liver Resection

2021 ◽  
Author(s):  
Yusuke Nishi ◽  
Taku Aoki ◽  
Takayuki Shimizu ◽  
Shun Sato ◽  
Takatsugu Matsumoto ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Reverse Transcriptase ◽  
Telomerase Reverse Transcriptase ◽  
Liver Carcinogenesis ◽  
Dependent Protein Kinase ◽  
Free Survival ◽  
Poor Tumor Differentiation ◽  
Tumor Tissues ◽  
Dependent Protein ◽  
Tert Expression

Abstract Telomerase reverse transcriptase (TERT) is reportedly expressed in various types of cancer. However, the significance of TERT expression in hepatocellular carcinoma (HCC) has not been fully evaluated. Herein, we evaluated TERT expression in resected HCC tumor tissues using immunohistochemistry. TERT expression was assessed in both the cytoplasm and the nucleus of HCC cells. The relationships between TERT expression and clinical characteristics were investigated. Among the 135 HCCs, TERT expression was positive only in the cytoplasm in 86 tumors (63.7%), was positive only in the nucleus in 3 tumors (2.2%), was positive in both the cytoplasm and the nucleus in 5 tumors (3.7%) and was negative in 41 tumors (30.4%). Cytoplasmic TERT expression was significantly associated with HBs antigen, poor tumor differentiation, and DNA-dependent protein kinase catalytic unit (DNA-PKcs) expression. However, TERT expression in the cytoplasm or in the nucleus was not significantly correlated with the overall or the recurrence-free survival periods. In conclusion, TERT was mainly expressed in the cytoplasm of HCC tissues. Cytoplasmic TERT expression is closely associated with HBV-related HCC and DNA-PKcs expression. The overexpression of TERT and DNA-PKcs arise from cccDNA and may be related to liver carcinogenesis in the presence of HBV infection.

1207-P: Tracing Telomerase Reverse Transcriptase (Tert) Expression to a Dormant Preadipogenic Origin

Diabetes ◽  
2021 ◽  
Vol 70 (Supplement 1) ◽  
pp. 1207-P
Author(s):  
MATTHEW LYNES ◽  
DIANA L. CARLONE ◽  
KRISTY L. TOWNSEND ◽  
DAVID BREAULT ◽  
YU-HUA TSENG
Keyword(s):  
Reverse Transcriptase ◽  
Telomerase Reverse Transcriptase ◽  
Tert Expression

scholarly journals CDCA3 Is a Novel Prognostic Biomarker Associated with Immune Infiltration in Hepatocellular Carcinoma

2021 ◽  
Vol 2021 ◽  
pp. 1-19
Author(s):  
Zhihuai Wang ◽  
Shuai Chen ◽  
Gaochao Wang ◽  
Sun Li ◽  
Xihu Qin
Keyword(s):  
Hepatocellular Carcinoma ◽  
T Cells ◽  
Immune Cell ◽  
Disease Free Survival ◽  
In Silico Analysis ◽  
Gene Markers ◽  
Free Survival ◽  
Immune Infiltration ◽  
Normal Tissues ◽  
Tumor Tissues

Cell division cycle-associated protein-3 (CDCA3) contributes to the regulation of the cell cycle. CDCA3 plays an important role in the carcinogenesis of various cancers; however, the association between CDCA3 expression, prognosis of patients, and immune infiltration in the tumor microenvironment is still unknown. Here, we demonstrated that CDCA3 was differentially expressed between the tumor tissues and corresponding normal tissues using in silico analysis in the ONCOMINE and Tumor Immune Estimation Resource (TIMER) databases. We analyzed the relationship between the expression of CDCA3 and prognosis of patients with hepatocellular carcinoma (HCC) using the Kaplan–Meier plotter database and Gene Expression Profiling Interactive Analysis (GEPIA). Furthermore, we determined the prognostic value of CDCA3 expression using univariate and multivariate analyses. We observed that CDCA3 expression closely correlated with immune infiltration and gene markers of infiltrating immune cells in the TIMER database. CDCA3 was highly expressed in the tumor tissues than in the adjacent normal tissues in various cancers, including HCC. Increased expression of CDCA3 was accompanied by poorer overall survival (OS), relapse-free survival (RFS), progression-free survival (PFS), and disease-specific survival (DSS). The correlation between CDCA3 expression and OS and disease-free survival (DFS) was also studied using GEPIA. CDCA3 expression was associated with the levels of immune cell infiltration and was positively correlated with tumor purity. Moreover, CDCA3 expression was associated with gene markers such as PD-1, CTLA4, LAG3, and TIM-3 from exhausted T cells, CD3D, CD3E, and CD2 from T cells, and TGFB1 and CCR8 located on the surface of Tregs. Thus, we demonstrated that CDCA3 may be a potential target and biomarker for the management and diagnosis of HCC.

Telomerase reverse transcriptase gene amplification in hepatocellular carcinoma

2004 ◽  
Vol 19 (11) ◽  
pp. 1300-1304 ◽  
Author(s):  
YOSHITAKA TAKUMA ◽  
KAZUHIRO NOUSO ◽  
YOSHIYUKI KOBAYASHI ◽  
SHINICHIRO NAKAMURA ◽  
HIRONORI TANAKA ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Reverse Transcriptase ◽  
Gene Amplification ◽  
Telomerase Reverse Transcriptase ◽  
Reverse Transcriptase Gene
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