scholarly journals Melanin Production and Laccase Mediated Oxidative Stress Alleviation During Fungal-fungal Interaction Among Basidiomycetes Fungi

2021 ◽  
Author(s):  
Samim Dullah ◽  
Dibya Jyoti Hazarika ◽  
Gunajit Goswami ◽  
Tanushree Borgohain ◽  
Alokesh Ghosh ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Metabolite Profile ◽  
Laccase Production ◽  
Melanin Production ◽  
Potential Implication ◽  
Hyphal Morphology ◽  
Stress Related Genes ◽  
Laccase Enzyme ◽  
The One ◽  
Increased Activity

Abstract Fungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction - Trametes coccinea (F3) with Leiotrametes lactinea (F9) and Trametes coccinea (F3) with Trametes versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of Trametes coccinea (F3) with Leiotrametes lactinea (F9) and Trametes coccinea (F3) with Trametes versicolor (F1) was analysed through Liquid Chromatography coupled to Mass Spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the change in the hyphal morphology of the one participating fungus. The bipartite fungal interaction resulted in the production of a dark brown colour pigment – melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal-fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal– fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production.

scholarly journals Melanin production and laccase mediated oxidative stress alleviation during fungal-fungal interaction among basidiomycete fungi

IMA Fungus ◽  
2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Samim Dullah ◽  
Dibya Jyoti Hazarika ◽  
Gunajit Goswami ◽  
Tanushree Borgohain ◽  
Alokesh Ghosh ◽  
...  
Keyword(s):  
Metabolite Profile ◽  
Laccase Production ◽  
Melanin Production ◽  
Potential Implication ◽  
Brown Colour ◽  
Hyphal Morphology ◽  
Stress Related Genes ◽  
Laccase Enzyme ◽  
Increased Activity ◽  
Basidiomycete Fungi

AbstractFungal-fungal interaction often leads to the change in metabolite profile of both the interacting fungus which may have potential implication in industry or agriculture. In the present study, we performed two sets of fungal-fungal interaction—Trametes coccinea (F3) with Leiotrametes lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) to understand the changes in the metabolite profile during the interaction process and how this process impacts the hyphal/mycelial morphology of the participating fungi. The metabolites produced during interaction of T. coccinea (F3) with L. lactinea (F9) and T. coccinea (F3) with T. versicolor (F1) was analysed through liquid chromatography coupled to mass spectroscopy (LC-MS). Most of the metabolites secreted or produced during interaction are associated with defensive response. Further, visualization with scanning electron microscopy revealed that interaction between the tested fungi led to the changes in the hyphal morphology. The bipartite fungal interaction resulted in the production of a dark brown colour pigment—melanin as confirmed by the LC-MS, FTIR and NMR analysis. Moreover, the fungal–fungal interaction also led to increase in the production of laccase, a group of multicopper oxidases involved in detoxification of toxic compounds. Further, increased activity of superoxide dismutase, an enzyme that catalyzes the dismutation of the superoxide anion to hydrogen peroxide was also recorded during fungal–fungal interaction. Quantitative real-time PCR revealed upregulation of lcc1 (encoding a laccase enzyme) and few other stress related genes of T. versicolor during its hyphal interaction with T. coccinea, suggesting a direct correlation between laccase production and melanin production.

scholarly journals Chitosan multiple addition enhances laccase production from Trametes versicolor

2015 ◽  
Vol 38 (10) ◽  
pp. 1973-1981 ◽  
Author(s):  
Abiodun Emmanuel Adekunle ◽  
Feng Wang ◽  
Jianhua Hu ◽  
Anzhou Ma ◽  
Chen Guo ◽  
...  
Keyword(s):  
Trametes Versicolor ◽  
Laccase Production ◽  
Multiple Addition

scholarly journals Characterization of highly active 2-keto-3-deoxy-L-arabinonate and 2-keto-3-deoxy-D-xylonate dehydratases in terms of the biotransformation of hemicellulose sugars to chemicals

2020 ◽  
Vol 104 (16) ◽  
pp. 7023-7035
Author(s):  
Samuel Sutiono ◽  
Bettina Siebers ◽  
Volker Sieber
Keyword(s):  
Racemic Mixture ◽  
Highly Active ◽  
Key Points ◽  
Deoxy Sugar ◽  
Weimberg Pathway ◽  
One Step ◽  
The One ◽  
Increased Activity ◽  
Enzyme Class

Abstract2-keto-3-L-arabinonate dehydratase (L-KdpD) and 2-keto-3-D-xylonate dehydratase (D-KdpD) are the third enzymes in the Weimberg pathway catalyzing the dehydration of respective 2-keto-3-deoxy sugar acids (KDP) to α-ketoglutaric semialdehyde (KGSA). The Weimberg pathway has been explored recently with respect to the synthesis of chemicals from L-arabinose and D-xylose. However, only limited work has been done toward characterizing these two enzymes. In this work, several new L-KdpDs and D-KdpDs were cloned and heterologously expressed in Escherichia coli. Following kinetic characterizations and kinetic stability studies, the L-KdpD from Cupriavidus necator (CnL-KdpD) and D-KdpD from Pseudomonas putida (PpD-KdpD) appeared to be the most promising variants from each enzyme class. Magnesium had no effect on CnL-KdpD, whereas increased activity and stability were observed for PpD-KdpD in the presence of Mg2+. Furthermore, CnL-KdpD was not inhibited in the presence of L-arabinose and L-arabinonate, whereas PpD-KdpD was inhibited with D-xylonate (I50 of 75 mM), but not with D-xylose. Both enzymes were shown to be highly active in the one-step conversions of L-KDP and D-KDP. CnL-KdpD converted > 95% of 500 mM L-KDP to KGSA in the first 2 h while PpD-KdpD converted > 90% of 500 mM D-KDP after 4 h. Both enzymes in combination were able to convert 83% of a racemic mixture of D,L-KDP (500 mM) after 4 h, with both enzymes being specific toward the respective stereoisomer. Key points• L-KdpDs and D-KdpDs are specific toward L- and D-KDP, respectively.• Mg2+affected activity and stabilities of D-KdpDs, but not of L-KdpDs.• CnL-KdpD and PpD-KdpD converted 0.5 M of each KDP isomer reaching 95 and 90% yield.• Both enzymes in combination converted 0.5 M racemic D,L-KDP reaching 83% yield.

scholarly journals ON THE OCCURRENCE OF CATALASE IN HUMAN TISSUES AND ITS VARIATIONS IN DISEASES

1908 ◽  
Vol 10 (6) ◽  
pp. 759-781 ◽  
Author(s):  
M. C. Winternitz ◽  
C. R. Meloy
Keyword(s):  
Catalytic Activity ◽  
Clinical Symptoms ◽  
Congenital Syphilis ◽  
Marked Change ◽  
The Other ◽  
Human Tissues ◽  
Subsequent Work ◽  
Simple Method ◽  
The One ◽  
Increased Activity

1. In work along this line it is most important to have a simple method of determining the catalytic activity of tissues. The method must be such that several observations can be made during a single experiment, so that a better idea can be obtained of the velocity of the reaction at various intervals. 2. The catalytic activity of human tissues varies greatly in diseases. A. Nephritis.—The kidney always shows the most marked reduction although the other tissues examined, blood, lung, liver, spleen, likewise show decrease in their power of decomposing hydrogen peroxide. This reduction varies directly with the severity of the pathological lesion in the kidney and the clinical symptoms. The urine also in cases of nephritis shows a much greater inhibiting power than normal urine. This may be accounted for by the reaction of the urine, and subsequent work must prove whether or not the kidney takes any more active part in nephritis and secretes into the blood and urine a substance which manifests itself by a reduction in the catalytic activity. B. The catalytic activity of the blood in the two cases of eclampsia which we have studied was not reduced. This is the most important fact we have so far obtained, if it can be substantiated, since it can furnish us with a ready ante-mortem means of differentiating eclampsia and nephritis. C.Pneumonia.—The lung in the stage of red hepatization has an increased catalytic activity. This increase varies directly with the number of intact red blood cells in the exudate, and in the engorged capillaries. Strength is given to this conclusion by the fact, that on the one hand, there is no increased activity in gray hepatization, while on the other hand, there is an enormously increased activity in the fresh hemorrhagic infarct. D. Tuberculosis.—The decreased activity of the lung in tuberculosis is probably due, for the most part, to the lack of blood in the diseased area, while the lowered activity which is present in the other organs is to be explained by the anæmia and emaciation which accompanies the process. Whether there is a specific catalytic inhibiting substance generated by the process, has not been determined. E. There was no reduction of the catalytic activity in the cases of diabetes mellitus and jaundice studied. F. In the one case of asphyxiation by illuminating gas, there was decided decrease in the catalytic activity of the blood. G. The tissues in the one case of congenital syphilis showed a marked lowering of the catalytic activity. 3. There is a slight decrease in the catalytic activity of the tissues due to post-mortem change, but this is so slight that it is hardly to be taken into consideration in the interpretation of the results obtained. 4. There is no marked change in the catalytic activity due to age. In concluding, we wish to thank Dr. A. S. Loevenhart, at whose suggestion this enzyme was studied, for the unfailing interest with which he has followed the progress of this work, and for the many valuable suggestions he has given us.

Screening of supports and inducers for laccase production by Trametes versicolor in semi-solid-state conditions

2002 ◽  
Vol 38 (2) ◽  
pp. 249-255 ◽  
Author(s):  
Susana Rodrı́guez Couto ◽  
Marı́a Gundı́n ◽  
Miriam Lorenzo ◽  
M.Ángeles Sanromán
Keyword(s):  
Solid State ◽  
Trametes Versicolor ◽  
Laccase Production ◽  
Semi Solid

Heat Shock Treatment Improves Trametes versicolor Laccase Production

2012 ◽  
Vol 168 (2) ◽  
pp. 256-265 ◽  
Author(s):  
Feng Wang ◽  
Chen Guo ◽  
Tao Wei ◽  
Tian Zhang ◽  
Chun-Zhao Liu
Keyword(s):  
Heat Shock ◽  
Trametes Versicolor ◽  
Shock Treatment ◽  
Laccase Production ◽  
Heat Shock Treatment

Lignin-Enhanced Laccase Production from Trametes versicolor

2016 ◽  
Vol 8 (4) ◽  
pp. 1061-1066 ◽  
Author(s):  
Abiodun E. Adekunle ◽  
Chen Guo ◽  
Chun-Zhao Liu
Keyword(s):  
Trametes Versicolor ◽  
Laccase Production

Polarisation cathodique en milieu alcalin des alliages Nb–Co

1981 ◽  
Vol 59 (8) ◽  
pp. 1261-1266 ◽  
Author(s):  
Y. Umetsu ◽  
D. L. Piron ◽  
G. Bélanger
Keyword(s):  
Solid Solution ◽  
Optical Microscopy ◽  
Hydrogen Evolution ◽  
Hydrogen Evolution Reaction ◽  
Cathodic Polarization ◽  
Electrochemical Activity ◽  
Surface Degradation ◽  
Δ Phase ◽  
The One ◽  
Increased Activity

NbCo alloys were prepared and their composition characterized by optical microscopy. These alloys were evaluated for their electrochemical activity towards the hydrogen evolution reaction (HER) at 25 °C in a 25% KOH solution. The alloys between 20 and 70wt% Co showed an increased activity compared to their parent metals. However the activity is below the one observed for pure Ni. As for the NbNi alloys, the NbCo undergo some surface degradation under prolonged (6 h) cathodic polarization at 400–700 mA cm−2. This disintegration of the surfaces is observed for alloys below 50% in Co. Above this proportion, the alloys remain stable towards the RDH. This degradation is associated with the presence of the δ phase in the alloy: this phase is the solid solution of Nb in the alloy.

scholarly journals Characterization of antioxidant properties and metabolite profile of Agave atrovirens extracts

2017 ◽  
Author(s):  
Victor Olvera-Garcia ◽  
Ana Belen Granado-Serrano ◽  
Mariona Jove ◽  
Anna Cassanye ◽  
Anaberta Cardador-Martinez ◽  
...  
Keyword(s):  
Oxidative Damage ◽  
Crude Extract ◽  
Antioxidant Properties ◽  
Antioxidant Defense System ◽  
Metabolite Profile ◽  
Antioxidant Response ◽  
Nuclear Extracts ◽  
Antioxidant Response Elements ◽  
Cell Culture Assay ◽  
The One

Agave spp. is widely analyzed because several functional properties have been described. Some minor bioactive compounds such as polyphenols, saponins and Maillard compounds produced during extraction procedures have been reported to exert antioxidant properties. The objective of this study was to elucidate the antioxidant properties of three different Agave atrovirens extracts in a HepG2 cell culture assay. The three extracts analyzed mostly showed antioxidant properties with an increase in NRF2 content in nuclear extracts. However, a differential response was observed in the reduction of protein oxidative damage in the three extracts analyzed, the crude extract being the one that mainly induced a reduction in oxidative damage. Metabolomic analysis was performed to elucidate the potential molecules responsible for the antioxidant properties, where 2-amino-4-methylphenol could be the main candidate responsible for inducing the transcription of cellular antioxidant response elements. It could be concluded that crude extract of Agave atrovirens may increase the cellular antioxidant defense system, with a reduction in oxidative damage.

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