scholarly journals Wide Spectrum Analysis of HPV Genotypes in External Anogenital Warts among Hungarian Patients

2021 ◽  
Author(s):  
Orsolya Rideg ◽  
Angéla Oszter ◽  
Evelin Makk ◽  
Endre Kálmán ◽  
Tamás Tornóczky ◽  
...  
Keyword(s):  
High Risk ◽  
Wide Spectrum ◽  
Low Risk ◽  
Anogenital Warts ◽  
Vaccination Programs ◽  
Hpv Dna ◽  
Formalin Fixed Paraffin ◽  
Hpv Genotypes ◽  
Formalin Fixed Paraffin Embedded ◽  
The Many

Abstract Background: External anogenital warts (EGW) are primarily associated with the low-risk Human papillomavirus (HPV) genotype 6 and 11, though coinfection with other low-risk and oncogenic high-risk HPV genotypes also occurs. Though the many studies about HPV-associated disease, the epidemiology, and the prevalence of HPV genotypes associated with EGW are not well characterized. The objective of our study was to determine the incidence of HPV genotypes in a Hungarian cohort diagnosed with EGW.Methods: A total number of 94 patients’ archived formalin-fixed paraffin-embedded (FFPE) blocks were enrolled in our retrospective study. HPV genotypes were determined, applying HPV Direct Flow Chip Test.Results: The overall prevalence of HPV DNA in EGW cases was 100%. 72.34% of the cases were mono while 27.65% were multi infections. Cumulative prevalence of HPV 6 and 11 was 98.93%; while a total of 19.4% of the cases occurred with at least one high-risk genotype. The most frequent HPV genotypes were HPV 6 (86.17%), 11 (12.76%), 42 (6.38%) followed by HPV 40, 56, 59, 66, 73 (3.19%).Conclusion: Data of our study could provide invaluable information concerning the relative frequencies of HPV types in the Hungarian population, enabling improved assessment of the actual and future efficacy of vaccination programs and forecast changes in infection patterns.

scholarly journals Wide Spectrum Analysis of Human Papillomavirus Genotypes in External Anogenital Warts

Vaccines ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 604
Author(s):  
Orsolya Rideg ◽  
Angéla Oszter ◽  
Evelin Makk ◽  
Endre Kálmán ◽  
Kornélia Farkas ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Vaccine Development ◽  
Wide Spectrum ◽  
Low Risk ◽  
Anogenital Warts ◽  
Vaccination Programs ◽  
Hpv Dna ◽  
Hpv Genotypes ◽  
Formalin Fixed Paraffin Embedded

External anogenital warts (EGW) are primarily associated with the low-risk human papillomavirus (HPV) genotypes 6 and 11, though coinfection with other low-risk and oncogenic high-risk HPV genotypes also occurs. Although there have been many studies on HPV-associated disease, the prevalence of HPV genotypes associated with EGW is not well characterized. The objective of our retrospective study was to determine the prevalence of HPV genotypes among patients diagnosed with EGW in the south-west of Hungary. Archived formalin-fixed paraffin-embedded tissues from 94 patients were processed in our study. HPV genotypes were determined, applying HPV Direct Flow CHIP test. The overall prevalence of HPV DNA in the EGW samples was 100%, yielding 131 infections among the 94 samples. Of these cases, 72.3% were mono while 27.6% were multi-infections. Out of the 131 infections, the cumulative prevalence of HPV 6 and 11 was 71%. A total of 98.9% of the samples were carrying at least one of these genotypes, while 19.1% of the cases occurred with at least one high-risk genotype. Data from our study could provide invaluable information concerning the prevalence of HPV types among patients with EGW, enabling improved assessment of the actual and future efficacy of vaccination programs, vaccine development, and forecast changes in infection patterns.

scholarly journals Retrospective Analysis of Prevalence of High-risk and Low-risk Human Papillomavirus (HPV) Genotypes in Iranian Women During 2013-2016

2017 ◽  
Vol 2 (4) ◽  
pp. 85-90 ◽  
Author(s):  
Siavash Chalabiani ◽  
Mina Khodadad Nazari ◽  
Mahdi Shabani ◽  
Neda Razavi Davoodi ◽  
Abdolfattah Sarafnejad ◽  
...  
Keyword(s):  
High Risk ◽  
Age Groups ◽  
Hpv Infection ◽  
Low Risk ◽  
Age Group ◽  
Cancer Etiology ◽  
Cross Sectional ◽  
Hpv Dna ◽  
Hpv Prevalence ◽  
Hpv Genotypes

Background and objective: Considering the importance of HPV in cancer etiology, awareness of HPV prevalence and frequency of high-risk genotypes could help improve health care system management. We analyzed HPV prevalence in women forms different provinces of Iran that is the largest sample till now. Material and Methods: In this retrospective cross-sectional study, 2969 outpatient and suspicious women aged 17- 78 referred to Noor pathobiology laboratory from 24 provinces of Iran were studies. DNA extraction and PCR were performed on samples and then HPV genotypes were also determined using hybridization kit. Results: HPV DNA was detected in 29.3% of valid liquid-based samples. Among HPV positive cases, 67.2% and 52.0% accounted for high-risk and low-risk HPV subtypes, respectively. In patients with high-risk HPV types, HPV16 was confirmed as predominate type (30.5%) followed by HPV53 (17.3%) and HPV39 (13.3 %). HPV6 was found as the most common low-risk HPV type with 60.6% frequency rate followed by HPV11 (17.9%) and HPV81 (8.6%) in this age group. It was apparent that age group >25 years accounted the highest frequency of HPV positivity. The prevalence of HPV was significantly different among different age groups (p<0.0001). Conclusion: It can be concluded that HPV infection is currently at a considerable high level in Iran. Looking at the high risk and oncogenic HPV subtypes frequency especially in younger age groups, a concern could be noticed about HPV relevant cancers which can be prevented by commercial and approved HPV vaccines.

Laboratory-Developed L1 Sequencing and Type-Specific, Real-Time Polymerase Chain Reaction for the Detection and Typing of Human Papillomaviruses in Formalin-Fixed, Paraffin-Embedded Tissues

2013 ◽  
Vol 137 (1) ◽  
pp. 50-54 ◽  
Author(s):  
Anne Mills ◽  
Rajeev Balasubramaniam ◽  
Teri A. Longacre ◽  
Christina S. Kong ◽  
Benjamin A. Pinsky
Keyword(s):  
Polymerase Chain Reaction ◽  
High Risk ◽  
Real Time ◽  
Clinical Laboratory ◽  
Low Risk ◽  
Chain Reaction ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Polymerase Chain ◽  
Formalin Fixed

Context.—The detection and typing of high-risk and low-risk human papillomavirus (HPV) in archival formalin-fixed, paraffin-embedded tissues by nucleic acid amplification testing is an important adjunct to immunohistochemical staining in evaluation of squamous cell proliferations of the oropharynx, larynx, and anal canal. Objective.—To evaluate semiautomated, xylene-free extraction from formalin-fixed, paraffin-embedded tissues combined with laboratory-developed HPV L1 sequencing and type-specific HPV 6, 11, 16, and 18 real-time polymerase chain reaction for identification and typing of HPV in the clinical laboratory. Design.—We evaluated the adequacy of extraction using β-globin amplification and compared L1 sequencing and real-time polymerase chain reaction methods for typing accuracy using 68 formalin-fixed, paraffin-embedded tissues, including 56 anorectal biopsy or surgical resection specimens and 12 laryngeal papilloma specimens from patients with recurrent respiratory papillomatosis. Results.—Adequate DNA was obtained from 68 of 68 specimens analyzed and all were HPV positive. In 47 cases where L1 sequencing demonstrated that the predominant HPV type was 6, 11, 16, or 18, type-specific, real-time polymerase chain reaction provided concordant results. Sequencing revealed additional low-risk (HPV 40) and high-risk HPV types (HPV 31, 33, 56, and 58) in anorectal specimens, whereas HPV 6 or 11 were the types found in laryngeal papillomas. Conclusion.—Both L1 sequencing and type-specific, real-time polymerase chain reaction are suitable methods for routine HPV testing of formalin-fixed, paraffin-embedded tissues in a clinical laboratory setting.

scholarly journals Incorporation of Cervista Human Papillomavirus 16/18 Assay Into Algorithms for Classifying Human Papillomavirus Status in Formalin-Fixed, Paraffin-Embedded Head and Neck Squamous Carcinoma Specimens

2018 ◽  
Vol 143 (3) ◽  
pp. 356-361
Author(s):  
Ming Guo ◽  
Abha Khanna ◽  
Jianping Wang ◽  
Michelle D. Williams ◽  
Neda Kalhor ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Human Papillomavirus ◽  
Ffpe Tissue ◽  
Hpv 16 ◽  
Hpv Dna ◽  
Formalin Fixed Paraffin ◽  
Hpv Status ◽  
Formalin Fixed Paraffin Embedded ◽  
Hpv Assays ◽  
Formalin Fixed

Context.— Human papillomavirus (HPV) DNA in situ hybridization (ISH) assay and p16 immunohistochemistry (IHC) are used to determine high-risk HPV status in formalin-fixed, paraffin-embedded (FFPE) tissues in oropharyngeal squamous cell carcinoma (SCC). Although high sensitivity and specificity for HPV can be obtained by combined p16 IHC and HPV DNA ISH, the occasional discrepancy between these assays has prompted evaluation of Cervista HPV assays in FFPE tissue from patients with oropharyngeal SCC. Objective.— To compare the efficacy of Cervista HPV 16/18 and Cervista HPV HR assay to that of HPV DNA ISH assay and p16 IHC in FFPE tissue in head and neck squamous cell carcinoma of oropharyngeal origin. Design.— Archived FFPE tissue from 84 patients with SCC of oropharyngeal origin and available HPV DNA ISH and p16 IHC test results were tested with the Cervista HPV 16/18 assay and further verified by polymerase chain reaction (PCR)–based HPV16/18 genotyping tests in cases with discrepancy. Results.— Of the 84 specimens, 75% (63 of 84) were positive and 16% (13 of 84) had discrepant or equivocal findings by p16 IHC and HPV DNA ISH testing. Use of Cervista HPV assays, either to clarify discrepant/equivocal findings or as confirmation after initial p16 IHC/HPV DNA ISH tests, identified 81% (68 of 84) of HPV-positive cases without equivocal HPV results. Five of 13 cases with discrepancy or equivocal HPV DNA ISH results tested positive for HPV16 or HPV18 by Cervista HPV 16/18 assay, which was further confirmed by PCR-based HPV 16/18 genotyping. Conclusions.— The Cervista HPV assays are a reasonable alternative to HPV DNA ISH in determining HPV status in FFPE tissue specimens from patients with oropharyngeal SCC.

Sensation Seeking among High and Low-Risk Male Athletes

1982 ◽  
Vol 4 (3) ◽  
pp. 246-253 ◽  
Author(s):  
William F. Straub
Keyword(s):  
High Risk ◽  
Sport Psychology ◽  
Discriminant Function ◽  
Sensation Seeking ◽  
Low Risk ◽  
Male Athletes ◽  
The Many ◽  
Sensation Seeking Scale ◽  
Discriminant Function Analyses

Many problems are associated with the measurement of athletes in contemporary sport psychology. There is, for example, a dearth of valid and reliable tests to assess the many and diverse behaviors of players. The purpose of this investigation was to attempt to validate Zuckerman's sensation seeking scale (SSS V) using high- and low-risk sport participants. The SSS (Form V) was administered to male hang gliders (n = 33), automobile racers (n = 22), and intercollegiate bowlers (n = 25). It was hypothesized that the high-risk athletes (hang gliders and auto racers) would score significantly higher (.05 level) than the low-risk sport participants (bowlers) in total sensation seeking score and on the four subscales of Zuckerman's test. Stepwise multiple discriminant function analyses found that except for thrill and adventure seeking and disinhibition subscales, the above hypotheses were tenable. Thus, it was concluded that support exists for Zuckerman's SSS (Form V) as a measure of sensation seeking among male athletes.

High-risk Human Papillomavirus Determination in Formalin-fixed, Paraffin-embedded Cervical Tissue Using the Roche Cobas 4800 System

2019 ◽  
Vol 38 (1) ◽  
pp. 11-16 ◽  
Author(s):  
Juan C. Tardío ◽  
Olivia Cambero ◽  
Carolina Sánchez-Estévez ◽  
Ana B. Sánchez-García ◽  
Fernando Angulo ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Cervical Tissue ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed ◽  
Roche Cobas

Detection of human papillomavirus DNA in self-administered vaginal swabs as compared to cervical swabs

2003 ◽  
Vol 14 (8) ◽  
pp. 560-567 ◽  
Author(s):  
Melanie E Palmisano ◽  
Ann M Gaffga ◽  
Jennifer Daigle ◽  
Joeli Brinkman ◽  
Kristina Mire ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Phase I ◽  
Phase Ii ◽  
Pap Smear ◽  
Blot Hybridization ◽  
Epidemiological Data ◽  
Hpv Dna ◽  
Vaginal Swabs ◽  
Hpv Genotypes

The primary risk factor for cervical cancer is infection with high-risk genotypes of human papillomavirus (HPV). This study compared HPV DNA detection between cervical swabs (CX) and self-administered vaginal swabs (SV). Phase I participants were 199 women chosen from a study comparing the detection of Chlamydia trachomatis from various anogenital sites. Phase II participants were 135 women from either the Colposcopy or HIV Outpatient Clinic. HPV DNA testing was performed using polymerase chain reaction and Roche reverse line blot hybridization. In Phase I samples, more CX samples amplified and more HPV genotypes ( P < 0.05) were detected in CX. Genotype 52 were seen more in the cervix, whereas genotype 82 (MM4) was detected solely in the vagina. The presence of high-risk HPV genotypes in the cervix was a predictor of an abnormal Papanicolaou (Pap) smear. In Phase II samples, CX samples amplified more, but similar rates of HPV genotypes were seen in SV and CX samples. Higher concordance rates of high-risk genotypes were seen in Phase II compared to Phase I samples. Phase II demonstrated the feasibility of utilizing SV sampling to reflect cervical status. If validated, a self-vaginal swab method to detect cervical HPV DNA status could be utilized to triage women with indeterminate Pap smears and be a useful method to collect epidemiological data from large populations.

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