scholarly journals Myocardial Remodeling in Ischemic Heart Disease – Imaging from Cell to Organ Level by Synchrotron Propagation Based X-Ray Phase-Contrast Imaging

2021 ◽  
Author(s):  
Ivo Planinc ◽  
Patricia Garcia-Canadilla ◽  
Hector Dejea ◽  
Ivana Ilic ◽  
Eduard Guasch ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Imaging Modality ◽  
Left Ventricular ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
Organ Level

Abstract Background: Cardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail.The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction.Methods and Results: Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8µm and 0.65µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas.Conclusions: Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

scholarly journals Comprehensive assessment of myocardial remodeling in ischemic heart disease by synchrotron propagation based X-ray phase contrast imaging

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ivo Planinc ◽  
Patricia Garcia-Canadilla ◽  
Hector Dejea ◽  
Ivana Ilic ◽  
Eduard Guasch ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Imaging Modality ◽  
Left Ventricular ◽  
Comprehensive Assessment ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray

AbstractCardiovascular research is in an ongoing quest for a superior imaging method to integrate gross-anatomical information with microanatomy, combined with quantifiable parameters of cardiac structure. In recent years, synchrotron radiation-based X-ray Phase Contrast Imaging (X-PCI) has been extensively used to characterize soft tissue in detail. The objective was to use X-PCI to comprehensively quantify ischemic remodeling of different myocardial structures, from cell to organ level, in a rat model of myocardial infarction. Myocardial infarction-induced remodeling was recreated in a well-established rodent model. Ex vivo rodent hearts were imaged by propagation based X-PCI using two configurations resulting in 5.8 µm and 0.65 µm effective pixel size images. The acquired datasets were used for a comprehensive assessment of macrostructural changes including the whole heart and vascular tree morphology, and quantification of left ventricular myocardial thickness, mass, volume, and organization. On the meso-scale, tissue characteristics were explored and compared with histopathological methods, while microstructural changes were quantified by segmentation of cardiomyocytes and calculation of cross-sectional areas. Propagation based X-PCI provides detailed visualization and quantification of morphological changes on whole organ, tissue, vascular as well as individual cellular level of the ex vivo heart, with a single, non-destructive 3D imaging modality.

scholarly journals The Quantification of Myocardial remodelling in a Rat Model of Myocardial Infarction by Synchrotron X-ray Phase Contrast Imaging

2018 ◽  
Vol 13 (11-12) ◽  
pp. 433-434
Author(s):  
Ivo Planinc ◽  
Patricia Garcia-Canadilla ◽  
Hector Dejea ◽  
Eduard Guasch ◽  
Marco Stampanoni ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Rat Model ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Contrast Imaging ◽  
X Ray ◽  
Myocardial Remodelling

A non-free-space propagation x-ray phase contrast imaging method sensitive to phase effects in two directions simultaneously

2009 ◽  
Vol 94 (4) ◽  
pp. 044108 ◽  
Author(s):  
A. Olivo ◽  
S. E. Bohndiek ◽  
J. A. Griffiths ◽  
A. Konstantinidis ◽  
R. D. Speller
Keyword(s):  
Free Space ◽  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray

scholarly journals Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron RadiationThree-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

2020 ◽  
Author(s):  
Qiang Tao ◽  
Chen-Chen Gao ◽  
Xue-Hong Tong ◽  
Shizhen Yuan ◽  
Tian-tian Wang ◽  
...  
Keyword(s):  
Synchrotron Radiation ◽  
Phase Contrast ◽  
Three Dimensional ◽  
Image Resolution ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Rat Stomach ◽  
Contrast Imaging ◽  
X Ray ◽  
3 Dimensional

Abstract Objectives This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using X-ray in-line phase-contrast imaging (XILPCI). The aim of the study was to demonstrate that XILPCI is a micron imaging method for gastric structures. Methods The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution. Results The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastroduodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future. Keywords: Synchrotron radiation phase-contrast imaging, 3-dimensional gastric structure images

scholarly journals Tomographic reconstruction using tilted Laue analyser-based X-ray phase-contrast imaging

2021 ◽  
Vol 28 (1) ◽  
pp. 283-291
Author(s):  
M. C. Chalmers ◽  
M. J. Kitchen ◽  
K. Uesugi ◽  
G. Falzon ◽  
P. Quin ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Tomographic Reconstruction ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
Phase Gradient ◽  
X Ray ◽  
Sensitive Phase ◽  
Phase Contrast Method ◽  
Phase Sensitive

Analyser-based phase-contrast imaging (ABPCI) is a highly sensitive phase-contrast imaging method that produces high-contrast images of weakly absorbing materials. However, it is only sensitive to phase gradient components lying in the diffraction plane of the analyser crystal [i.e. in one dimension (1-D)]. In order to accurately account for and measure phase effects produced by the wavefield-sample interaction, ABPCI and other 1-D phase-sensitive methods must achieve 2-D phase gradient sensitivity. An inclined geometry method was applied to a Laue geometry setup for X-ray ABPCI through rotation of the detector and object about the optical axis. This allowed this traditionally 1-D phase-sensitive phase-contrast method to possess 2-D phase gradient sensitivity. Tomographic datasets were acquired over 360° of a multi-material phantom with the detector and sample tilted by 8°. The real and imaginary parts of the refractive index were reconstructed for the phantom.

scholarly journals Applications of a non-interferometric x-ray phase contrast imaging method with both synchrotron and conventional sources

2013 ◽  
Vol 8 (05) ◽  
pp. C05008-C05008 ◽  
Author(s):  
M Endrizzi ◽  
P C Diemoz ◽  
P R T Munro ◽  
C K Hagen ◽  
M B Szafraniec ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray

scholarly journals Edge-illumination X-ray phase contrast imaging: matching the imaging method to the detector technology

2014 ◽  
Vol 9 (11) ◽  
pp. C11004-C11004 ◽  
Author(s):  
M. Endrizzi ◽  
P.C. Diemoz ◽  
C.K. Hagen ◽  
F.A. Vittoria ◽  
P.R.T. Munro ◽  
...  
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Imaging ◽  
Imaging Method ◽  
Contrast Imaging ◽  
X Ray ◽  
Detector Technology

scholarly journals Synchrotron- and laboratory-based X-ray phase-contrast imaging for imaging mouse articular cartilage in the absence of radiopaque contrast agents

2014 ◽  
Vol 372 (2010) ◽  
pp. 20130127 ◽  
Author(s):  
Massimo Marenzana ◽  
Charlotte K. Hagen ◽  
Patricia Das Neves Borges ◽  
Marco Endrizzi ◽  
Magdalena B. Szafraniec ◽  
...  
Keyword(s):  
Articular Cartilage ◽  
Soft Tissue ◽  
Phase Contrast ◽  
Ex Vivo ◽  
Phase Changes ◽  
Phase Contrast Imaging ◽  
Laboratory System ◽  
Micro Ct ◽  
Contrast Imaging ◽  
X Ray

The mouse model of osteoarthritis (OA) has been recognized as the most promising research tool for the identification of new OA therapeutic targets. However, this model is currently limited by poor throughput, dependent on the extremely time-consuming histopathology assessment of the articular cartilage (AC). We have recently shown that AC in the rat tibia can be imaged both in air and in saline solution using a laboratory system based on coded-aperture X-ray phase-contrast imaging (CAXPCi). Here, we explore ways to extend the methodology for imaging the much thinner AC of the mouse, by means of gold-standard synchrotron-based phase-contrast methods. Specifically, we have used analyser-based phase-contrast micro-computed tomography (micro-CT) for its high sensitivity to faint phase changes, coupled with a high-resolution (4.5 μm pixel) detector. Healthy, diseased (four weeks post induction of OA) and artificially damaged mouse AC was imaged at the Elettra synchrotron in Trieste, Italy, using the above method. For validation, we used conventional micro-CT combined with radiopaque soft-tissue staining and standard histomorphometry. We show that mouse cartilage can be visualized correctly by means of the synchrotron method. This suggests that: (i) further developments of the laboratory-based CAXPCi system, especially in terms of pushing the resolution limits, might have the potential to resolve mouse AC ex vivo and (ii) additional improvements may lead to a new generation of CAXPCi micro-CT scanners which could be used for in vivo longitudinal pre-clinical imaging of soft tissue at resolutions impossible to achieve by current MRI technology.

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