scholarly journals iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

2020 ◽  
Author(s):  
Junliang Li ◽  
Jie Cui ◽  
Dayou Cheng ◽  
Cuihong Dai ◽  
Tianjiao Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Information Processing ◽  
Sugar Beet ◽  
Molecular Mechanism ◽  
Beta Vulgaris ◽  
Protein Profile ◽  
Betaine Aldehyde Dehydrogenase ◽  
Higher Plant ◽  
Metabolism Regulation ◽  
Leaves And Roots

Abstract Background Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in Beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of salt-tolerant sugar beet. Results Here, leaves and roots were used to identify the differentially abundant protein species (DAPs) between salt-stress and control conditions in beta vulgaris . As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H + -transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data. Conclusions Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.

scholarly journals iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

2020 ◽  
Author(s):  
Junliang Li ◽  
Jie Cui ◽  
Dayou Cheng ◽  
Cuihong Dai ◽  
Tianjiao Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Information Processing ◽  
Sugar Beet ◽  
Molecular Mechanism ◽  
Beta Vulgaris ◽  
Protein Profile ◽  
Betaine Aldehyde Dehydrogenase ◽  
Higher Plant ◽  
Metabolism Regulation ◽  
Leaves And Roots

Abstract Background Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of beet salt-tolerant. Results Here, leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data. Conclusions Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.

scholarly journals iTRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots

2020 ◽  
Author(s):  
Junliang Li ◽  
Jie Cui ◽  
Dayou Cheng ◽  
Cuihong Dai ◽  
Tianjiao Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Higher Plants ◽  
Profile Analysis ◽  
Protein Profile ◽  
Salt Resistance ◽  
Betaine Aldehyde Dehydrogenase ◽  
Proteomics Data ◽  
Metabolism Regulation ◽  
Leaves And Roots

Abstract Background: Salinity is one of the most serious threats to world agriculture. An important sugar-yielding crop sugar beet, which shows some tolerance to salt via a mechanism that is poorly understood. Proteomics data can provide important clues that can contribute to finally understand this mechanism.Results: Differentially abundant proteins (DAPs) in sugar beet under salt stress treatment were identified in leaves (70 DAPs) and roots (76 DAPs). Functions of these DAPs were predicted, and included metabolism and cellular, environmental information and genetic information processing. We hypothesize that these processes work in concert to maintain cellular homeostasis. Some DAPs are closely related to salt resistance, such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expression pattern of ten DAPs encoding genes was consistent with the iTRAQ data.Conclusions: During sugar beet adaptation to salt stress, leaves and roots cope using distinct mechanisms of molecular metabolism regulation. This study provides significant insights into the molecular mechanism underlying the response of higher plants to salt stress, and identified some candidate proteins involved in salt stress countermeasures.

scholarly journals iTRAQ protein profile analysis of sugar beet under salt stress: different coping mechanisms in leaves and roots

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Junliang Li ◽  
Jie Cui ◽  
Dayou Cheng ◽  
Cuihong Dai ◽  
Tianjiao Liu ◽  
...  
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Profile Analysis ◽  
Protein Profile ◽  
Coping Mechanisms ◽  
Leaves And Roots

Proteome analysis of sugar beet (Beta vulgaris L.) elucidates constitutive adaptation during the first phase of salt stress

2011 ◽  
Vol 168 (6) ◽  
pp. 519-526 ◽  
Author(s):  
Abdul Wakeel ◽  
Abdul R. Asif ◽  
Britta Pitann ◽  
Sven Schubert
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Proteome Analysis

Hydrolytic and pumping activity of H+-ATPase from leaves of sugar beet (Beta vulgaris L.) as affected by salt stress

2010 ◽  
Vol 167 (9) ◽  
pp. 725-731 ◽  
Author(s):  
Abdul Wakeel ◽  
Stefan Hanstein ◽  
Britta Pitann ◽  
Sven Schubert
Keyword(s):  
Salt Stress ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Pumping Activity

The expression patterns of plasma membrane aquaporins in leaves of sugar beet and its halophyte relative, Beta vulgaris ssp. maritima, in response to salt stress

Biologia ◽  
2015 ◽  
Vol 70 (4) ◽  
Author(s):  
Monika Skorupa-Kłaput ◽  
Joanna Szczepanek ◽  
Katarzyna Kurnik ◽  
Andrzej Tretyn ◽  
Jarosław Tyburski
Keyword(s):  
Plasma Membrane ◽  
Salt Stress ◽  
Sugar Beet ◽  
Beta Vulgaris ◽  
Water Status ◽  
Expression Patterns ◽  
Salt Solutions ◽  
Short Term ◽  
Long Term Treatment

AbstractSalt tolerance is largely dependent on a plant’s ability to maintain optimal water status in leaves. The adjustment of water relations under salinity involves changes in the transcriptional activity of genes encoding plasma membrane aquaporins (PIPs). Here, we report the effects of long-term or short-term treatments with moderate or strong salt stress on the expression of BvPIP1;1, BvPIP2;1 and BvPIP2;2 in the leaves of sugar beet, Beta vulgaris cv. Huzar, and its halophyte relative, Beta vulgaris ssp. maritima. Plants subjected to long-term treatment were watered with salt-supplemented media during a 32 day long culture period. Short-term salt treatments were executed either by immersing the petioles of excised leaves into salt solutions for 48h, or incubating excised leaf blades in salt-supplemented media for 20h. B. vulgaris ssp. maritima reacted to long-term salt treatment with a decrease in BvPIP1;1, BvPIP2;1 and BvPIP2;2 expression. Contrastingly, only BvPIP2;2 transcript was down-regulated by salinity in leaves of B. vulgaris cv. Huzar, whereas BvPIP1;1 and BvPIP2;1 did not vary in response to salt-treatments. On the other hand, the expression of BvPIP1;1, BvPIP2;1 and BvPIP2;2 was enhanced by salinity if salt solutions was supplied through leaf petioles, irrespective of genotype. PIP expression in excised leaf blades revealed a complex pattern of changes. BvPIP1;1 and BvPIP2;1 expression underwent a period of transient increase in both the control and salt-treated leaves. Furthermore, BvPIP1;1 expression was enhanced by strong salinity. BvPIP2;2 expression was up-regulated by strong salinity or up- or down-regulated by moderate salinity during the treatment period.

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