Effect of halotolerant rhizobacteria isolated from halophytes on the growth of sugar beet (Beta vulgaris L.) under salt stress

AbstractSalt tolerance is largely dependent on a plant’s ability to maintain optimal water status in leaves. The adjustment of water relations under salinity involves changes in the transcriptional activity of genes encoding plasma membrane aquaporins (PIPs). Here, we report the effects of long-term or short-term treatments with moderate or strong salt stress on the expression of BvPIP1;1, BvPIP2;1 and BvPIP2;2 in the leaves of sugar beet, Beta vulgaris cv. Huzar, and its halophyte relative, Beta vulgaris ssp. maritima. Plants subjected to long-term treatment were watered with salt-supplemented media during a 32 day long culture period. Short-term salt treatments were executed either by immersing the petioles of excised leaves into salt solutions for 48h, or incubating excised leaf blades in salt-supplemented media for 20h. B. vulgaris ssp. maritima reacted to long-term salt treatment with a decrease in BvPIP1;1, BvPIP2;1 and BvPIP2;2 expression. Contrastingly, only BvPIP2;2 transcript was down-regulated by salinity in leaves of B. vulgaris cv. Huzar, whereas BvPIP1;1 and BvPIP2;1 did not vary in response to salt-treatments. On the other hand, the expression of BvPIP1;1, BvPIP2;1 and BvPIP2;2 was enhanced by salinity if salt solutions was supplied through leaf petioles, irrespective of genotype. PIP expression in excised leaf blades revealed a complex pattern of changes. BvPIP1;1 and BvPIP2;1 expression underwent a period of transient increase in both the control and salt-treated leaves. Furthermore, BvPIP1;1 expression was enhanced by strong salinity. BvPIP2;2 expression was up-regulated by strong salinity or up- or down-regulated by moderate salinity during the treatment period.

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Genome-Wide Identification of Na+/H+ Antiporter (NHX) Genes in Sugar Beet (Beta vulgaris L.) and Their Regulated Expression under Salt Stress

Genes ◽

10.3390/genes10050401 ◽

2019 ◽

Vol 10 (5) ◽

pp. 401 ◽

Cited By ~ 11

Author(s):

Guo-Qiang Wu ◽

Jin-Long Wang ◽

Shan-Jia Li

Keyword(s):

Salt Stress ◽

Sugar Beet ◽

Beta Vulgaris ◽

Molecular Mechanisms ◽

Expression Patterns ◽

Pcr Analysis ◽

Functional Identification ◽

Interacting Protein ◽

Protein Protein Interaction ◽

Moderate Salinity

Salinity is one of the major environment factors that limits the growth of plants and the productivity of crops worldwide. It has been shown that Na+ transporters play a central role in salt tolerance and development of plants. The objective of this study was to identify Na+/H+ antiporter (NHX) genes and investigate their expression patterns in sugar beet (Beta vulgaris L.) subjected to various concentrations of NaCl. A total of five putative NHX genes were identified and distributed on four chromosomes in sugar beet. Phylogenetic analysis revealed that these BvNHX genes are grouped into three major classes, viz Vac- (BvNHX1, -2 and -3), Endo- (BvNHX4), and PM-class NHX (BvNHX5/BvSOS1), and within each class the exon/intron structures are conserved. The amiloride-binding site is found in TM3 at N-terminus of Vac-class NHX proteins. Protein-protein interaction (PPI) prediction suggested that only BvNHX5 putatively interacts with calcineurin B-like proteins (CBL) and CBL-interacting protein kinases (CIPK), implying it might be the primary NHX involved in CBL-CIPK pathway under saline condition. It was also found that BvNHX5 contains one abscisic acid (ABA)-responsive element (ABRE), suggesting that BvNHX5 might be involved in ABA signal responsiveness. Additionally, the qRT-PCR analysis showed that all the BvNHX genes in both roots and leaves are significantly up-regulated by salt, and the transcription levels under high salinity are significantly higher than those under either low or moderate salinity. Taken together, this work gives a detailed overview of the BvNHX genes and their expression patterns under salt stress. Our findings also provide useful information for elucidating the molecular mechanisms of Na+ homeostasis and further functional identification of the BvNHX genes in sugar beet.

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Growth and Gas Exchange Response of Sugar Beet (Beta vulgaris L.) Cultivars Grown Under Salt Stress

Photosynthesis. Energy from the Sun ◽

10.1007/978-1-4020-6709-9_308 ◽

2008 ◽

pp. 1431-1434 ◽

Cited By ~ 1

Author(s):

Alireza Dadkhah ◽

Seyed Hashem Moghtader

Keyword(s):

Salt Stress ◽

Gas Exchange ◽

Sugar Beet ◽

Beta Vulgaris

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Effect of long term salt stress on gas exchange and leaf carbohydrate contents in two sugar beet (Beta vulgaris L.) cultivars

Russian Agricultural Sciences ◽

10.3103/s1068367415060038 ◽

2015 ◽

Vol 41 (6) ◽

pp. 423-428

Author(s):

Alireza Dadkhah

Keyword(s):

Salt Stress ◽

Gas Exchange ◽

Sugar Beet ◽

Beta Vulgaris ◽

Carbohydrate Contents

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iTRAQ protein profile analysis of leaves and roots of sugar beet (Beta vulgaris) differing in response to salt stress

10.21203/rs.3.rs-17371/v1 ◽

2020 ◽

Author(s):

Junliang Li ◽

Jie Cui ◽

Dayou Cheng ◽

Cuihong Dai ◽

Tianjiao Liu ◽

...

Keyword(s):

Salt Stress ◽

Information Processing ◽

Sugar Beet ◽

Molecular Mechanism ◽

Beta Vulgaris ◽

Protein Profile ◽

Betaine Aldehyde Dehydrogenase ◽

Higher Plant ◽

Metabolism Regulation ◽

Leaves And Roots

Abstract Background Salinity is one of the most serious threat to agriculture worldwide. Sugar beet is an important sugar-yielding crop and has a certain tolerance to salt. However, the molecular mechanism of salt tolerance in beta vulgaris are poorly understood. Proteomics can provide a new perspective and deeper understanding for the research of beet salt-tolerant. Results Here, leaves and roots were used to identify the differentially abundant protein species between salt-stress and control conditions in beta vulgaris. As a result, 70 and 76 DAPs were identified in leaves and roots, respectively. The functions were determined for the classification of the DAPs, mainly involved in cellular processes, environmental information processing, genetic information processing and metabolism. These processes can work cooperatively to reconstruct the favorable equilibrium of physiological and cellular homeostasis under salt stress. Some candidate DAPs are closely related to salt resistance such as choline monooxygenase, betaine aldehyde dehydrogenase, glutathione S-transferase (GST) and F-type H+-transporting ATPase. The expressional pattern of 10 DAPs encoding genes were consistent with the iTRAQ data. Conclusions Our results demonstrated that during adaptation of beet to salt stress, leaves and roots have distinct mechanisms of molecular metabolism regulation. This study provided some significative insights into the molecular mechanism underlying the response of higher plant to salt stress, and identified some candidate proteins against salt stress.

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