scholarly journals Molecular characterization of a novel polymycovirus from the phytopathogenic fungus Setosphaeria turcica

2021 ◽  
Author(s):  
Zhongnan Gao ◽  
Manyu Zhang ◽  
Tingting Yu ◽  
Xiaoyan Wang ◽  
Xin Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Novel Species ◽  
Plasmopara Viticola ◽  
Phytopathogenic Fungus ◽  
Phylogeny Reconstruction ◽  
Setosphaeria Turcica ◽  
Sequence Identity ◽  
A Genome ◽  
The Family

Abstract A putative polymycovirus tentatively named Setosphaeria turcica polymycovirus 1 (StPmV1) was discovered from the phytopathogenic fungus Setosphaeria turcica. StPmV1 has a genome comprising 5 double stranded RNAs (dsRNAs). DsRNA1-3 each encodes a protein sharing significant but lower than 64% sequence identity with corresponding proteins from other polymycoviruses. DsRNA4-5 each encodes a protein with a sequence not conserved among polymycoviral proteins. However, the protein encoded by dsRNA4 is rich in proline (P), alanine (A), and serine (S) residues, which is a feature shared by the so-called PAS-rich proteins encoded by all polymycoviruses. Phylogeny reconstruction using the RNA-dependent RNA polymerases (RdRp) of accepted or putative polymycoviruses revealed that StPmV1 is most closely related to Plasmopara viticola lesion associated Polymycovirusmyco 1 (PvaPolymyco1), a putative polymycovirus recovered from the phytopathogenic oomycetes Plasmopara viticola. These data suggest that StPmV1 may represent a novel species of the genus Polymycovirus under the family Polymycoviridae. To our knowledge, this is the first polymycovirus reported from S. turcica.

scholarly journals Molecular characterization of a novel polymycovirus from the phytopathogenic fungus Setosphaeria turcica

2021 ◽  
Author(s):  
Zhongnan Gao ◽  
Manyu Zhang ◽  
Tingting Yu ◽  
Xiaoyan Wang ◽  
Xin Wang ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Phytopathogenic Fungus ◽  
Setosphaeria Turcica

Molecular characterization of a novel endornavirus from the phytopathogenic fungus Botrytis cinerea

2016 ◽  
Vol 162 (1) ◽  
pp. 313-316 ◽  
Author(s):  
Fangmin Hao ◽  
Ziliang Zhou ◽  
Mingde Wu ◽  
Guoqing Li
Keyword(s):  
Botrytis Cinerea ◽  
Molecular Characterization ◽  
Phytopathogenic Fungus

scholarly journals Molecular characterization of a novel mycovirus of the family Tymoviridae isolated from the plant pathogenic fungus Fusarium graminearum

Virology ◽  
2016 ◽  
Vol 489 ◽  
pp. 86-94 ◽  
Author(s):  
Pengfei Li ◽  
Yanhong Lin ◽  
Hailong Zhang ◽  
Shuangchao Wang ◽  
Dewen Qiu ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Fusarium Graminearum ◽  
Pathogenic Fungus ◽  
Plant Pathogenic Fungus ◽  
The Family

scholarly journals Characterization of a new picornavirus isolated from the freshwater fish Lepomis macrochirus

2014 ◽  
Vol 95 (3) ◽  
pp. 601-613 ◽  
Author(s):  
Marisa Barbknecht ◽  
Sol Sepsenwol ◽  
Eric Leis ◽  
Maren Tuttle-Lau ◽  
Mark Gaikowski ◽  
...  
Keyword(s):  
Freshwater Fish ◽  
New Genus ◽  
Lepomis Macrochirus ◽  
Leader Peptide ◽  
Fish Kill ◽  
Sequence Identity ◽  
Phylogenetic Cluster ◽  
The Family ◽  
Sport Fish

The freshwater fish Lepomis macrochirus (bluegill) is common to North American waters, and important both ecologically and as a sport fish. In 2001 an unknown virus was isolated from bluegills following a bluegill fish kill. This virus was identified as a picornavirus [termed bluegill picornavirus (BGPV)] and a diagnostic reverse transcriptase PCR was developed. A survey of bluegills in Wisconsin waters showed the presence of BGPV in 5 of 17 waters sampled, suggesting the virus is widespread in bluegill populations. Experimental infections of bluegills confirmed that BGPV can cause morbidity and mortality in bluegills. Molecular characterization of BGPV revealed several distinct genome characteristics, the most unusual of which is the presence of a short poly(C) tract in the 3′ UTR. Additionally, the genome encodes a polyprotein lacking a leader peptide and a VP0 maturation cleavage site, and is predicted to encode two distinct 2A proteins. Sequence comparison showed that the virus is most closely related to a phylogenetic cluster of picornaviruses that includes the genera Aquamavirus, Avihepatovirus and Parechovirus. However, it is distinct enough, for example sharing only about 38 % sequence identity to the parechoviruses in the 3D region, that it may represent a new genus in the family Picornaviridae.

scholarly journals Candida dubliniensis sp. nov.: phenotypic and molecular characterization of a novel species associated with oral candidosis in HIV-infected individuals

Microbiology ◽  
1995 ◽  
Vol 141 (7) ◽  
pp. 1507-1521 ◽  
Author(s):  
Derek J. Sullivan ◽  
Ken A. Haynes ◽  
Désirée E. Bennett ◽  
David C. Coleman ◽  
Thea J. Westerneng
Keyword(s):  
Molecular Characterization ◽  
Novel Species ◽  
Candida Dubliniensis ◽  
Oral Candidosis

scholarly journals Molecular characterization of the largest mycoviral-like double-stranded RNAs associated with Amasya cherry disease, a disease of presumed fungal aetiology

2006 ◽  
Vol 87 (10) ◽  
pp. 3113-3117 ◽  
Author(s):  
Z. Kozlakidis ◽  
L. Covelli ◽  
F. Di Serio ◽  
A. Citir ◽  
S. Açıkgöz ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Unknown Function ◽  
Functional Relationship ◽  
Rna Polymerases ◽  
Partial Characterization ◽  
The Family

The sequence of the four large (L) double-stranded RNAs (dsRNAs) associated with Amasya cherry disease (ACD), which has a presumed fungal aetiology, is reported. ACD L dsRNAs 1 (5121 bp) and 2 (5047 bp) potentially encode proteins of 1628 and 1620 aa, respectively, that are 37 % identical and of unknown function. ACD L dsRNAs 3 (4458 bp) and 4 (4303 bp) potentially encode proteins that are 68 % identical and contain the eight motifs conserved in RNA-dependent RNA polymerases (RdRp) of dsRNA mycoviruses, having highest similarity with those of members of the family Totiviridae. Both terminal regions share extensive conservation in all four RNAs, suggesting a functional relationship between them. As ACD L dsRNAs 1 and 2 do not encode RdRps, both are probably replicated by those from either ACD L dsRNA 3 or 4. Partial characterization of the equivalent L dsRNAs 3 and 4 associated with cherry chlorotic rusty spot revealed essentially identical sequences.

Molecular characterization of a virus from the family Luteoviridae associated with cotton blue disease

2005 ◽  
Vol 150 (7) ◽  
pp. 1357-1367 ◽  
Author(s):  
R. L. Corrêa ◽  
T. F. Silva ◽  
J. L. Simões-Araújo ◽  
P. A. V. Barroso ◽  
M. S. Vidal ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
The Family ◽  
Cotton Blue ◽  
Cotton Blue Disease

scholarly journals IND-6, a Highly Divergent IND-Type Metallo-β-Lactamase from Chryseobacterium indologenes Strain 597 Isolated in Burkina Faso

2009 ◽  
Vol 53 (10) ◽  
pp. 4320-4326 ◽  
Author(s):  
Boukaré Zeba ◽  
Filomena De Luca ◽  
Alain Dubus ◽  
Michael Delmarcelle ◽  
Jacques Simporé ◽  
...  
Keyword(s):  
Burkina Faso ◽  
Posttranslational Modification ◽  
Human Pathogens ◽  
Turnover Rates ◽  
Chryseobacterium Indologenes ◽  
Sequence Identity ◽  
New Variant ◽  
The Family ◽  
Identification And Characterization

ABSTRACT The genus Chryseobacterium and other genera belonging to the family Flavobacteriaceae include organisms that can behave as human pathogens and are known to cause different kinds of infections. Several species of Flavobacteriaceae, including Chryseobacterium indologenes, are naturally resistant to β-lactam antibiotics (including carbapenems), due to the production of a resident metallo-β-lactamase. Although C. indologenes presently constitutes a limited clinical threat, the incidence of infections caused by this organism is increasing in some settings, where isolates that exhibit multidrug resistance phenotypes (including resistance to aminoglycosides and quinolones) have been detected. Here, we report the identification and characterization of a new IND-type variant from a C. indologenes isolate from Burkina Faso that is resistant to β-lactams and aminoglycosides. The levels of sequence identity of the new variant to other IND-type metallo-β-lactamases range between 72 and 90% (for IND-4 and IND-5, respectively). The purified enzyme exhibited N-terminal heterogeneity and a posttranslational modification consisting of the presence of a pyroglutamate residue at the N terminus. IND-6 shows a broad substrate profile, with overall higher turnover rates than IND-5 and higher activities than IND-2 and IND-5 against ceftazidime and cefepime.

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