scholarly journals Rapid detection method of Skeletonema pseudocostatum and preparation of test strip

2021 ◽  
Author(s):  
Haoran Li ◽  
Kun Meng ◽  
Qiaojie Yu ◽  
Changping Chen ◽  
Jingfeng Chen ◽  
...  
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Red Tide ◽  
Health And Safety ◽  
Field Testing ◽  
Test Strip ◽  
Water Quality Monitoring ◽  
Red Tides ◽  
Easy Method ◽  
Color Development

Abstract In eutrophic waters, harmful algal blooms(HAB) are particularly prone to occur, which will affect the ecological environment and public health and safety. How to quickly detect and monitor marine microalgae is the key to preventing and managing HAB. Our innovative application of colloidal gold immunochromatography(GICG) technology to detect the dominant species in red tide, Skeletonema pseudocostatum, to monitor the outbreak of red tide. The experimental results show that the method and the prepared test strips are extremely sensitive, and can specifically detect the presence of Skeletonema pseudocostatum. The approximate concentration of algae cells is judged by establishing a fitting relationship between the degree of color development and the concentration of algae cells. This test strip provides a quick and easy method for routine environmental monitoring, fishery water quality monitoring, and field testing of red tide monitoring. It effectively warns of the outbreak of red tides and also provides a new application direction for GICG technology.

scholarly journals Tracing the sources of nutrients fueling dinoflagellate red tides occurring along the coast of Korea using radium isotopes

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Hyeong Kyu Kwon ◽  
Guebuem Kim ◽  
Yongjin Han ◽  
Junhyeong Seo ◽  
Weol Ae Lim ◽  
...  
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Red Tide ◽  
Subsurface Layer ◽  
Vertical Mixing ◽  
Eastern Coast ◽  
Nutrient Concentrations ◽  
Red Tides ◽  
Radium Isotopes ◽  
Nutrient Sources

Abstract It is a well held concept that the magnitude of red-tide occurrence is dependent on the amount of nutrient supply if the conditions are same for temperature, salinity, light, interspecific competition, etc. However, nutrient sources fueling dinoflagellate red-tides are difficult to identify since red tides usually occur under very low inorganic-nutrient conditions. In this study, we used short-lived Ra isotopes (223Ra and 224Ra) to trace the nutrient sources fueling initiation and spread of Cochlodinium polykrikoides blooms along the coast of Korea during the summers of 2014, 2016, and 2017. Horizontal and vertical distributions of nutrient concentrations correlated well with 224Ra activities in nutrient-source waters. The offshore red-tide areas showed high 224Ra activities and low-inorganic and high-organic nutrient concentrations, which are favorable for blooming C. polykrikoides in competition with diatoms. Based on Ra isotopes, the nutrients fueling red-tide initiation (southern coast of Korea) are found to be transported horizontally from inner-shore waters. However, the nutrients in the spread region (eastern coast of Korea), approximately 200 km from the initiation region, are supplied continuously from the subsurface layer by vertical mixing or upwelling. Our study highlights that short-lived Ra isotopes are excellent tracers of nutrients fueling harmful algal blooms in coastal waters.

scholarly journals Fish Kill Incidents and Harmful Algal Blooms in Omani Waters

2011 ◽  
Vol 16 ◽  
pp. 23 ◽  
Author(s):  
Hamed Mohammed Al Gheilani ◽  
Kazumi Matsuoka ◽  
Abdulaziz Yahya AlKindi ◽  
Shehla Amer ◽  
Colin Waring
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Red Tide ◽  
Ecological Impacts ◽  
Red Tides ◽  
Fish Kill ◽  
Noctiluca Scintillans ◽  
Trichodesmium Erythraeum ◽  
Toxic Species ◽  
Desalination Plants

Red tide, one of the harmful algal blooms (HABs) is a natural ecological phenomenon and often this event is accompanied by severe impacts on coastal resources, local economies, and public health. The occurrence of red tides has become more frequent in Omani waters in recent years. Some of them caused fish kill, damaged fishery resources and mariculture, threatened the marine environment and the osmosis membranes of desalination plants. However, a number of them have been harmless. The most common dinoflagellate Noctiluca scintillans is associated with the red tide events in Omani waters. Toxic species like Karenia selliformis, Prorocentrum arabianum, and Trichodesmium erythraeum have also been reported recently. Although red tides in Oman have been considered a consequence of upwelling in the summer season (May to September), recent phytoplankton outbreaks in Oman are not restricted to summer. Frequent algal blooms have been reported during winter (December to March). HABs may have contributed to hypoxia and/or other negative ecological impacts. 

Preliminary Development of a Competitive Fluorescent Quantum Dot-Based Immunochromatographic Test Strip for Sensitive Saxitoxin Detection

2020 ◽  
Vol 18 (6) ◽  
pp. 459-463
Author(s):  
John G. Bruno ◽  
Jeremy John
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Dynamic Range ◽  
Red Tide ◽  
Coupling Reaction ◽  
Test Strip ◽  
Paralytic Shellfish Poisoning ◽  
Shellfish Poisoning ◽  
Band Shift ◽  
Portable Detection

Rapid and portable detection of saxitoxin (STX) and its many congeners is highly desirable to prevent paralytic shellfish poisoning due to red tide or harmful algal blooms. In this work, we describe successful preliminary efforts to develop a very sensitive general STX family test strip employing highly fluorescent red quantum dots (Qdot 655) to detect as little as 0.5 to 1 part per billion (ppb or ng/ml) of STX with a dynamic range extending to 20,000 ppb after the prototype dipstick assay was optimized. A competitive format was necessitated by the small molecule nature of STXs having only one epitope, but the decrease in Qdot fluorescence was clearly visible to the naked eye as a function of increasing STX concentration in aqueous buffer. The competitive displacement assay format required conjugation of a primary amine in STX to carboxyl-Qdot 655 via a covalent carbodiimide coupling reaction which was validated by an electrophoretic mobility band shift assay.

scholarly journals Performance of a Handheld Chlorophyll-a Fluorometer: Potential Use for Rapid Algae Monitoring

Water ◽  
2021 ◽  
Vol 13 (10) ◽  
pp. 1409
Author(s):  
Hamdhani Hamdhani ◽  
Drew E. Eppehimer ◽  
David Walker ◽  
Michael T. Bogan
Keyword(s):  
Chlorophyll A ◽  
Surface Waters ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Water Quality Monitoring ◽  
Ambient Light ◽  
Calibration Equation ◽  
Field Samples ◽  
Potential Use

Chlorophyll-a measurements are an important factor in the water quality monitoring of surface waters, especially for determining the trophic status and ecosystem management. However, a collection of field samples for extractive analysis in a laboratory may not fully represent the field conditions. Handheld fluorometers that can measure chlorophyll-a in situ are available, but their performance in waters with a variety of potential light-interfering substances has not yet been tested. We tested a handheld fluorometer for sensitivity to ambient light and turbidity and compared these findings with EPA Method 445.0 using water samples obtained from two urban lakes in Tucson, Arizona, USA. Our results suggested that the probe was not sensitive to ambient light and performed well at low chlorophyll-a concentrations (<25 µg/L) across a range of turbidity levels (50–70 NTU). However, the performance was lower when the chlorophyll-a concentrations were >25 µg/L and turbidity levels were <50 NTU. To account for this discrepancy, we developed a calibration equation to use for this handheld fluorometer when field monitoring for potential harmful algal blooms in water bodies.

Meeting the Manatee Challenge: The Feasibility of Using Crittercam on Wild Manatees

2007 ◽  
Vol 41 (4) ◽  
pp. 14-18 ◽  
Author(s):  
Nicole M. Adimey ◽  
Kyler Abernathy ◽  
Joseph C. Gaspard, III ◽  
Greg Marshall
Keyword(s):  
Endangered Species ◽  
Data Collection ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Habitat Degradation ◽  
Field Testing ◽  
Imaging Data ◽  
Water Control ◽  
Productive Research ◽  
West Indian Manatee

The West Indian manatee, Trichechus manatus, is currently listed as an endangered species throughout its range. Threats to the subspecies in Florida include watercraft collisions, the potential loss of warm water refuges, exposure to harmful algal blooms, entanglement in fishery gear, habitat degradation, freshwater diversions and withdrawals, and the crushing or isolation caused by water control structures. A study was initiated in 2001 to investigate several of these threats and develop methods to aid in endangered species recovery needs by exploring the feasibility of using Crittercam, an animal-borne imaging and data-collection device, on Florida manatees. While the actual Crittercam technology was appropriate for the goals of this study, the unique morphology of the species and its variable habitat made finding a method of attachment extremely challenging. An appropriate and reliable method of attachment to the manatee is necessary for the successful application of animal-borne imaging data collection. Methods previously used successfully on other species were explored, including various combinations of suction cups and adhesives, a nylon harness and a peduncle belt with a trailing tether. Over a span of several years, captive trials and field testing determined that the peduncle belt with connecting tether was the most appropriate method of attachment. Several iterations of this design were made in order to provide the necessary functionality for ensuring productive research with the Crittercam on wild manatees.

scholarly journals Unsteady Multi-Element Time Series Analysis and Prediction Based on Spatial-Temporal Attention and Error Forecast Fusion

2020 ◽  
Vol 12 (2) ◽  
pp. 34
Author(s):  
Xiaofan Wang ◽  
Lingyu Xu
Keyword(s):  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Short Term Memory ◽  
Sudden Change ◽  
Back Propagation ◽  
Red Tides ◽  
Model Errors ◽  
Chl A ◽  
Error Forecast ◽  
Concentration Prediction

Harmful algal blooms (HABs) often cause great harm to fishery production and the safety of human lives. Therefore, the detection and prediction of HABs has become an important issue. Machine learning has been increasingly used to predict HABs at home and abroad. However, few of them can capture the sudden change of Chl-a in advance and handle the long-term dependencies appropriately. In order to address these challenges, the Long Short-Term Memory (LSTM) based spatial-temporal attentions model for Chlorophyll-a (Chl-a) concentration prediction is proposed, a model which can capture the correlation between various factors and Chl-a adaptively and catch dynamic temporal information from previous time intervals for making predictions. The model can also capture the stage of Chl-a when values soar as red tide breaks out in advance. Due to the instability of the current Chl-a concentration prediction model, the model is also applied to make a prediction about the forecast reliability, to have a basic understanding of the range and fluctuation of model errors and provide a reference to describe the range of marine disasters. The data used in the experiment is retrieved from Fujian Marine Forecasts Station from 2009 to 2011 and is combined into 8-dimension data. Results show that the proposed approach performs better than other Chl-a prediction algorithms (such as Attention LSTM and Seq2seq and back propagation). The result of error prediction also reveals that the error forecast method possesses established advantages for red tides prevention and control.

scholarly journals Harmful algal blooms and red tide problems on the U.S. west coast

1997 ◽  
Vol 42 (5part2) ◽  
pp. 1076-1088 ◽  
Author(s):  
Rita A. Horner ◽  
David L. Garrison ◽  
F. Gerald Plumley
Keyword(s):  
West Coast ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Red Tide ◽  
The U.S

scholarly journals Red Tides

EDIS ◽  
2008 ◽  
Vol 2008 (3) ◽  
Author(s):  
Jorge R. Rey
Keyword(s):  
Human Health ◽  
Harmful Algal Blooms ◽  
Algal Blooms ◽  
Marine Ecosystems ◽  
Red Tides ◽  
And Control

ENY-851, a 5-page illustrated factsheet by Jorge R. Rey, explains what these “harmful algal blooms” are, what causes them, their impacts on marine ecosystems, human health, and coastal economies, and strategies for mitigation and control. Includes references. Published by UF Entomology and Nematology Department, February 2008.

Sign in / Sign up

Export Citation Format

Share Document