scholarly journals Diversity and distribution of sulfate-reducing bacteria and its ecological effect in Shimen Realgar Mine

2021 ◽  
Author(s):  
Xianbin Zhu ◽  
Liyuan Chen ◽  
Hongzhong Pan ◽  
Lei Wang ◽  
Xun Zhang ◽  
...  
Keyword(s):  
Research Area ◽  
Ecological Effect ◽  
Sulfite Reductase ◽  
Sulfate Reducing ◽  
Microbial Sulfate Reduction ◽  
Dissimilatory Sulfite Reductase ◽  
New Type ◽  
The Rich ◽  
Reducing Bacteria ◽  
Insight Into

Abstract Microbial sulfate reduction, a vital mechanism for microorganisms living in anaerobic, sulfate-rich environments, is an essential aspect of the sulfur biogeochemical cycle. However, there has been no detailed investigation of the diversity and distribution of SRBs and its effect in Shimen Realgar Mine, which is characterized as a hot research area rich in sulfate. To elucidate this issue, soils sample from Shimen Realgar Mine were collected. Further, a total of 55 new or new-type dissimilatory sulfite reductase genes and 5 new families of DrsAB proteins were successfully identified, which demonstrate the rich and unique diversity of the sulfate reducing microbes in this environment. We also isolate a novel DSRP strain, Desulfotomaculum sp. JL90 from the soils, which can efficiently respiratory reduces sulfate and arsenate. JL90 also can promote the generation of yellow precipitations in the presence of multiple electron acceptors (both contain sulfate and As(V) in the cultures). Moreover, microbial community compositions also indicated the biogenesis contribution of SRBs to the Shimen realgar mine. The results of this study provided a new insight into the diversity and distribution of SRBs and its ecological effect in Shimen Realgar Mine.

scholarly journals Phylogeography of Sulfate-Reducing Bacteria among Disturbed Sediments, Disclosed by Analysis of the Dissimilatory Sulfite Reductase Genes (dsrAB)

2005 ◽  
Vol 71 (2) ◽  
pp. 1004-1011 ◽  
Author(s):  
J. R. Pérez-Jiménez ◽  
L. J. Kerkhof
Keyword(s):  
New York ◽  
Distribution Patterns ◽  
Sulfate Reducing Bacteria ◽  
Sulfite Reductase ◽  
Geographic Proximity ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Polluted Sites ◽  
Eukaryotic Organisms ◽  
Reducing Bacteria

ABSTRACT Sediment samples were collected worldwide from 16 locations on four continents (in New York, California, New Jersey, Virginia, Puerto Rico, Venezuela, Italy, Latvia, and South Korea) to assess the extent of the diversity and the distribution patterns of sulfate-reducing bacteria (SRB) in contaminated sediments. The SRB communities were examined by terminal restriction fragment (TRF) length polymorphism (TRFLP) analysis of the dissimilatory sulfite reductase genes (dsrAB) with NdeII digests. The fingerprints of dsrAB genes contained a total of 369 fluorescent TRFs, of which <20% were present in the GenBank database. The global sulfidogenic communities appeared to be significantly different among the anthropogenically impacted (petroleum-contaminated) sites, but nearly all were less diverse than pristine habitats, such as mangroves. A global SRB indicator species of petroleum pollution was not identified. However, several dsrAB gene sequences corresponding to hydrocarbon-degrading isolates or consortium members were detected in geographically widely separated polluted sites. Finally, a cluster analysis of the TRFLP fingerprints indicated that many SRB microbial communities were most similar on the basis of close geographic proximity (tens of kilometers). Yet, on larger scales (hundreds to thousands of kilometers) SRB communities could cluster with geographically widely separated sites and not necessarily with the site with the closest proximity. These data demonstrate that SRB populations do not adhere to a biogeographic distribution pattern similar to that of larger eukaryotic organisms, with the greatest species diversity radiating from the Indo-Pacific region. Rather, a patchy SRB distribution is encountered, implying an initially uniform SRB community that has differentiated over time.

scholarly journals Congruent Phylogenies of Most Common Small-Subunit rRNA and Dissimilatory Sulfite Reductase Gene Sequences Retrieved from Estuarine Sediments

2001 ◽  
Vol 67 (7) ◽  
pp. 3314-3318 ◽  
Author(s):  
Catherine Joulian ◽  
Niels B. Ramsing ◽  
Kjeld Ingvorsen
Keyword(s):  
Target Genes ◽  
Small Subunit ◽  
Estuarine Sediments ◽  
Sulfite Reductase ◽  
Small Subunit Rrna ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Phylogenetic Affiliation ◽  
Reductase Gene ◽  
Reducing Bacteria

ABSTRACT The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and producedDesulfosarcina variabilis-like sequences from the inoculum but Desulfomicrobium baculatum-like sequences from a high dilution in natural media. Related organisms were subsequently cultivated from the site. PCR bias appear to be limited (or very similar) for the two primersets and target genes. However, the DSR primers showed a much higher phylogenetic specificity. DSR gene analysis is thus a promising and specific approach for investigating SRB diversity in complex habitats.

scholarly journals Multiple Lateral Transfers of Dissimilatory Sulfite Reductase Genes between Major Lineages of Sulfate-Reducing Prokaryotes

2001 ◽  
Vol 183 (20) ◽  
pp. 6028-6035 ◽  
Author(s):  
Michael Klein ◽  
Michael Friedrich ◽  
Andrew J. Roger ◽  
Philip Hugenholtz ◽  
Susan Fishbain ◽  
...  
Keyword(s):  
Sulfite Reductase ◽  
Beta Subunits ◽  
Transfer Event ◽  
Sulfate Reducers ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Rrna Phylogeny ◽  
Diversity Studies ◽  
Reducing Bacteria ◽  
Reference Strains

ABSTRACT A large fragment of the dissimilatory sulfite reductase genes (dsrAB) was PCR amplified and fully sequenced from 30 reference strains representing all recognized lineages of sulfate-reducing bacteria. In addition, the sequence of thedsrAB gene homologs of the sulfite reducerDesulfitobacterium dehalogenans was determined. In contrast to previous reports, comparative analysis of all available DsrAB sequences produced a tree topology partially inconsistent with the corresponding 16S rRNA phylogeny. For example, the DsrAB sequences of several Desulfotomaculum species (low G+C gram-positive division) and two members of the genusThermodesulfobacterium (a separate bacterial division) were monophyletic with δ-proteobacterial DsrAB sequences. The most parsimonious interpretation of these data is thatdsrAB genes from ancestors of as-yet-unrecognized sulfate reducers within the δ-Proteobacteria were laterally transferred across divisions. A number of insertions and deletions in the DsrAB alignment independently support these inferred lateral acquisitions of dsrAB genes. Evidence for adsrAB lateral gene transfer event also was found within the δ-Proteobacteria, affecting Desulfobacula toluolica. The root of the dsr tree was inferred to be within the Thermodesulfovibrio lineage by paralogous rooting of the alpha and beta subunits. This rooting suggests that the dsrAB genes inArchaeoglobus species also are the result of an ancient lateral transfer from a bacterial donor. Although these findings complicate the use of dsrAB genes to infer phylogenetic relationships among sulfate reducers in molecular diversity studies, they establish a framework to resolve the origins and diversification of this ancient respiratory lifestyle among organisms mediating a key step in the biogeochemical cycling of sulfur.

scholarly journals Diversity of Sulfate-Reducing Bacteria in Oxic and Anoxic Regions of a Microbial Mat Characterized by Comparative Analysis of Dissimilatory Sulfite Reductase Genes

1999 ◽  
Vol 65 (10) ◽  
pp. 4666-4671 ◽  
Author(s):  
Dror Minz ◽  
Jodi L. Flax ◽  
Stefan J. Green ◽  
Gerard Muyzer ◽  
Yehuda Cohen ◽  
...  
Keyword(s):  
Comparative Analysis ◽  
Sequence Analysis ◽  
Sulfate Reducing Bacteria ◽  
Microbial Mat ◽  
Sulfite Reductase ◽  
Sulfate Reducing ◽  
Dissimilatory Sulfite Reductase ◽  
Genes Encoding ◽  
Reducing Bacteria

ABSTRACT Sequence analysis of genes encoding dissimilatory sulfite reductase (DSR) was used to identify sulfate-reducing bacteria in a hypersaline microbial mat and to evaluate their distribution in relation to levels of oxygen. The most highly diverse DSR sequences, most related to those of the Desulfonema-like organisms within the δ-proteobacteria, were recovered from oxic regions of the mat. This observation extends those of previous studies by us and others associating Desulfonema-like organisms with oxic habitats.

A comparison of the electrophoretic properties of the ATP-sulfurylases, APS-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria

1973 ◽  
Vol 19 (3) ◽  
pp. 375-380 ◽  
Author(s):  
G. W. Skyring ◽  
P. A. Trudinger
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Disc Electrophoresis ◽  
Sulfite Reductase ◽  
Electrophoretic Variation ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Reducing Bacteria ◽  
Atp Sulfurylases

ATP-sulfurylases, APS-reductases, and sulfite reductases (SO3−2 → S−2) have been detected by gel disc electrophoresis in 13 cultures of dissimilatory sulfate-reducing bacteria and their electrophoretic properties have been compared. With respect to these three enzymes only, the results were indicative of some interspecies and intergenus homologies. In the Desulfovibrio strains (except Desulfovibrio desulfuricans 8301 which does not contain desulfoviridin), the major sulfite reductase was electrophoretically coincident with desulfoviridin and, in the Desulfotomaculum strains, with a brown protein. Some distinct patterns of electrophoretically distinguishable forms of APS-reductase were found. Considerable electrophoretic variation was found among the ATP-sulfurylases.

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