A Liquid Chromatographic Method for Determination of Acetamiprid and Buprofezin Residues and Their Dissipation Kinetics in Paddy Matrices and Soil

Abstract The present study was conducted to investigate the residue status of two insecticides (acetamiprid and buprofezin) and their dissipation kinetics in three matrices viz. paddy grain, straw and soil. The extraction protocol for residues of these two insecticides was executed using acetonitrile solvent. The analytical method was validated, which showed good linearity with the limit of quantification (LOQ) value of 0.01 and 0.02 mg kg− 1 for acetamiprid and buprofezin respectively. The recovery range was 79.67–98.33 % concerning all the matrices in both the insecticides. Acetamiprid (20% SP) and Buprofezin (25% SC) were applied separately in the paddy field in two doses: single dose (recommended dose) and double dose along with untreated control throughout the experiment. Residue analysis of these two insecticides in paddy (grain and straw) and soil was accomplished employing high-pressure liquid chromatography (HPLC) with a UV detector. The dissipation data showed that acetamiprid exhibited higher dissipation in comparison with buprofezin. However, their persistence was found slightly higher in soil. The dissipation dynamics in the rice and soil were discussed with biological half-lives of both the insecticides. Consumer risk assessment study was also made considering its fate to the consumers.

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A Liquid Chromatographic Method for Determination of Acetamiprid and Buprofezin Residues and Their Dissipation Kinetics in Paddy Matrices and Soil

10.21203/rs.3.rs-225672/v1 ◽

2021 ◽

Author(s):

Sujan Majumder ◽

Somnath Mandal ◽

Biswajit Majumder ◽

Anindita Paul ◽

Tarun Paul ◽

...

Keyword(s):

Chromatographic Method ◽

Residue Analysis ◽

Double Dose ◽

Uv Detector ◽

Limit Of Quantification ◽

Dissipation Kinetics ◽

Assessment Study ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic

Abstract The present study was conducted to investigate the residue status of two insecticides (acetamiprid and buprofezin) and their dissipation kinetics in three matrices viz. paddy grain, straw and soil. The extraction protocol for residues of these two insecticides was executed using acetonitrile solvent. The analytical method was validated, which showed good linearity with the limit of quantification (LOQ) value of 0.01 and 0.02 mg kg-1 for acetamiprid and buprofezin respectively. The recovery range was 79.67-98.33 % concerning all the matrices in both the insecticides. Acetamiprid (20% SP) and Buprofezin (25% SC) were applied separately in the paddy field in two doses: single dose (recommended dose) and double dose along with untreated control throughout the experiment. Residue analysis of these two insecticides in paddy (grain and straw) and soil was accomplished employing high-pressure liquid chromatography (HPLC) with a UV detector. The dissipation data showed that acetamiprid exhibited higher dissipation in comparison with buprofezin. However, their persistence was found slightly higher in soil. The dissipation dynamics in the rice and soil were discussed with biological half-lives of both the insecticides. Consumer risk assessment study was also made considering its fate to the consumers.

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High performance liquid chromatographic method for the determination of guaifenesin in pharmaceutical syrups and in environmental samples

Baghdad Science Journal ◽

10.21123/bsj.10.3.1014-1022 ◽

2013 ◽

Vol 10 (3) ◽

pp. 1014-1022

Author(s):

Baghdad Science Journal

Keyword(s):

High Performance ◽

Chromatographic Method ◽

Limit Of Detection ◽

Industrial Effluent ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Limit Of Quantification ◽

Liquid Chromatographic Method ◽

Liquid Chromatographic

A simple, precise, rapid, and accurate reversed – phase high performance liquid chromatographic method has been developed for the determination of guaifenesin in pure from pharmaceutical formulations.andindustrial effluent. Chromatography was carried out on supelco L7 reversed- phase column (25cm × 4.6mm), 5 microns, using a mixture of methanol –acetonitrile-water: (80: 10 :10 v/v/v) as a mobile phase at a flow rate of 1.0 ml.min-1. Detection was performed at 254nm at ambient temperature. The retention time for guaifenesin was found 2.4 minutes. The calibration curve was linear (r= 0.9998) over a concentration range from 0.08 to 0.8mg/ml. Limit of detection (LOD) and limit of quantification ( LOQ) were found 6µg/ml and 18µg/ml respectively. The method was validated for its linearity, precision and accuracy .The proposed method was successfully applied for the determination of guaifenesin in syrups and industrial effluent samples.

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