Spike Architecture Traits Associated With Type II Resistance to Fusarium Head Blight in Bread Wheat

Abstract Fusarium head blight (FHB) remains a devastating disease in bread wheat (Triticum aestivum L.) and other small grains. Genetic resistance to FHB is a complex trait; in addition to active physiological resistance, plant developmental and morphological traits may indirectly affect disease progression and provide a passive mechanism of resistance. In this study, we investigated the relationship between FHB type II resistance and spike architecture traits in a recombinant inbred line (RIL) population of bread wheat. Disease resistance traits were FHB severity at 21 days post inoculation (dpi) and area under the disease progress curve (AUDPC). Spike architecture traits measured were rachis length, spike density, number of spikelets per spike, florets per spike and florets per spikelet. The RIL population showed significant variation for all traits. Heritability values were moderate to high for FHB severity (0.69) and AUDPC (0.63) and high for the spike architecture traits (0.74 - 0.92). FHB severity and AUDPC showed a moderate and significant association with the number of florets per spike (r = 0.38 and r = 0.31, respectively) and with the number of florets per spikelet (r = 0.28 and r = 0.27, respectively), reflecting a greater spread of the fungus in spikes with higher floret number. These results suggest that the number of florets per spike and the number of florets per spikelet should be considered in FHB resistance breeding efforts, because selection of lines with higher number of florets could lead to a correlated selection response towards increased FHB levels under field conditions.

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Spike architecture traits associated with type II resistance to fusarium head blight in bread wheat

Euphytica ◽

10.1007/s10681-021-02936-3 ◽

2021 ◽

Vol 217 (12) ◽

Author(s):

M. F. Franco ◽

G. A. Lori ◽

G. Cendoya ◽

M. P. Alonso ◽

J. S. Panelo ◽

...

Keyword(s):

Fusarium Head Blight ◽

Bread Wheat ◽

Type Ii ◽

Head Blight ◽

Type Ii Resistance

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Using anthesis date as a covariate to accurately assessing type II resistance to Fusarium head blight in field-grown bread wheat

Crop Protection ◽

10.1016/j.cropro.2020.105504 ◽

2021 ◽

Vol 142 ◽

pp. 105504

Author(s):

M.F. Franco ◽

G.A. Lori ◽

M.G. Cendoya ◽

J.S. Panelo ◽

M.P. Alonso ◽

...

Keyword(s):

Fusarium Head Blight ◽

Bread Wheat ◽

Type Ii ◽

Head Blight ◽

Type Ii Resistance

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Genome-wide association mapping revealed syntenic loci QFhb-4AL and QFhb-5DL for Fusarium head blight resistance in common wheat (Triticum aestivum L.)

10.21203/rs.2.12667/v3 ◽

2019 ◽

Author(s):

Wenjing Hu ◽

Derong Gao ◽

Hongya Wu ◽

Jian Liu ◽

Chunmei Zhang ◽

...

Keyword(s):

Genome Wide Association Study ◽

Resistance Breeding ◽

Snp Markers ◽

Genome Wide Association ◽

Type Ii ◽

Head Blight ◽

Genome Wide ◽

A Genome ◽

Fhb Resistance ◽

Type Ii Resistance

Abstract Background: Fusarium head blight (FHB), primarily caused by Fusarium graminearum, is a major threat to wheat production and food security worldwide. Breeding stably and durably resistant cultivars is the most effective approach for managing and controlling the disease. The success of FHB resistance breeding relies on identification of an effective resistant germplasm. We performed a genome-wide association study (GWAS) using the high-density wheat 90K single nucleotide polymorphism (SNP) assays to better understand the genetic basis of FHB resistance in natural population and identify associated molecular markers. Results: The resistance to FHB fungal spread along the rachis (Type II resistance) was evaluated on 171 wheat cultivars in the 2016-2017 (abbr. as 2017) and 2017-2018 (abbr. as 2018) growing seasons. Using Illumina Infinum iSelect 90K SNP genotyping data, a genome-wide association study (GWAS) identified 26 loci (88 marker-trait associations), which explained 6.65-14.18% of the phenotypic variances. The associated loci distributed across all chromosomes except 2D, 6A, 6D and 7D, with those on chromosomes 1B, 4A, 5D and 7A being detected in both years. New loci for Type II resistance were found on syntenic genomic regions of chromsome 4AL (QFhb-4AL, 621.85-622.24 Mb) and chromosome 5DL (QFhb-5DL, 546.09-547.27 Mb) which showed high collinearity in gene content and order. SNP markers wsnp_JD_c4438_5568170 and wsnp_CAP11_c209_198467 of 5D, reported previously linked to a soil-borne wheat mosaic virus (SBWMV) resistance gene, were also associated with FHB resistance in this study. Conclusion: The syntenic FHB resistant loci and associated SNP markers identified in this study are valuable for FHB resistance breeding via marker-assisted selection.

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Cell Wall Biomolecular Composition Plays a Potential Role in the Host Type II Resistance to Fusarium Head Blight in Wheat

Frontiers in Microbiology ◽

10.3389/fmicb.2016.00910 ◽

2016 ◽

Vol 7 ◽

Cited By ~ 14

Author(s):

Rachid Lahlali ◽

Saroj Kumar ◽

Lipu Wang ◽

Li Forseille ◽

Nicole Sylvain ◽

...

Keyword(s):

Cell Wall ◽

Fusarium Head Blight ◽

Potential Role ◽

Type Ii ◽

Head Blight ◽

Host Type ◽

Type Ii Resistance

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Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance)

Theoretical and Applied Genetics ◽

10.1007/s001220200009 ◽

2002 ◽

Vol 104 (1) ◽

pp. 84-91 ◽

Cited By ~ 313

Author(s):

H. Buerstmayr ◽

M. Lemmens ◽

L. Hartl ◽

L. Doldi ◽

B. Steiner ◽

...

Keyword(s):

Fusarium Head Blight ◽

Spring Wheat ◽

Molecular Mapping ◽

Fusarium Head Blight Resistance ◽

Blight Resistance ◽

Type Ii ◽

Head Blight ◽

Type Ii Resistance

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Characterizing Winter Wheat Germplasm for Fusarium Head Blight Resistance Under Accelerated Growth Conditions

Frontiers in Plant Science ◽

10.3389/fpls.2021.705006 ◽

2021 ◽

Vol 12 ◽

Author(s):

Mustafa Zakieh ◽

David S. Gaikpa ◽

Fernanda Leiva Sandoval ◽

Marwan Alamrani ◽

Tina Henriksson ◽

...

Keyword(s):

Winter Wheat ◽

Fusarium Head Blight ◽

Fusarium Head Blight Resistance ◽

Resistance Breeding ◽

Growth Conditions ◽

Head Blight ◽

Wheat Germplasm ◽

Progress Curve ◽

Accelerated Growth ◽

Fhb Resistance

Fusarium head blight (FHB) is one of the economically important diseases of wheat as it causes severe yield loss and reduces grain quality. In winter wheat, due to its vernalization requirement, it takes an exceptionally long time for plants to reach the heading stage, thereby prolonging the time it takes for characterizing germplasm for FHB resistance. Therefore, in this work, we developed a protocol to evaluate winter wheat germplasm for FHB resistance under accelerated growth conditions. The protocol reduces the time required for plants to begin heading while avoiding any visible symptoms of stress on plants. The protocol was tested on 432 genotypes obtained from a breeding program and a genebank. The mean area under disease progress curve for FHB was 225.13 in the breeding set and 195.53 in the genebank set, indicating that the germplasm from the genebank set had higher resistance to FHB. In total, 10 quantitative trait loci (QTL) for FHB severity were identified by association mapping. Of these, nine QTL were identified in the combined set comprising both genebank and breeding sets, while two QTL each were identified in the breeding set and genebank set, respectively, when analyzed separately. Some QTLs overlapped between the three datasets. The results reveal that the protocol for FHB evaluation integrating accelerated growth conditions is an efficient approach for FHB resistance breeding in winter wheat and can be even applied to spring wheat after minor modifications.

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Genome-wide association mapping revealed syntenic loci QFhb-4AL and QFhb-5DL for Fusarium head blight resistance in common wheat (Triticum aestivum L.)

10.21203/rs.2.12667/v4 ◽

2019 ◽

Author(s):

Wenjing Hu ◽

Derong Gao ◽

Hongya Wu ◽

Jian Liu ◽

Chunmei Zhang ◽

...

Keyword(s):

Genome Wide Association Study ◽

Resistance Breeding ◽

Snp Markers ◽

Genome Wide Association ◽

Type Ii ◽

Head Blight ◽

Genome Wide ◽

A Genome ◽

Fhb Resistance ◽

Type Ii Resistance

Abstract Background: Fusarium head blight (FHB), primarily caused by Fusarium graminearum, is a major threat to wheat production and food security worldwide. Breeding stably and durably resistant cultivars is the most effective approach for managing and controlling the disease. The success of FHB resistance breeding relies on identification of an effective resistant germplasm. We conducted a genome-wide association study (GWAS) using the high-density wheat 90K single nucleotide polymorphism (SNP) assays to better understand the genetic basis of FHB resistance in natural population and identify associated molecular markers. Results: The resistance to FHB fungal spread along the rachis (Type II resistance) was evaluated on 171 wheat cultivars in the 2016-2017 (abbr. as 2017) and 2017-2018 (abbr. as 2018) growing seasons. Using Illumina Infinum iSelect 90K SNP genotyping data, a genome-wide association study (GWAS) identified 26 loci (88 marker-trait associations), which explained 6.65-14.18% of the phenotypic variances. The associated loci distributed across all chromosomes except 2D, 6A, 6D and 7D, with those on chromosomes 1B, 4A, 5D and 7A being detected in both years. New loci for Type II resistance were found on syntenic genomic regions of chromsome 4AL (QFhb-4AL, 621.85-622.24 Mb) and chromosome 5DL (QFhb-5DL, 546.09-547.27 Mb) which showed high collinearity in gene content and order. SNP markers wsnp_JD_c4438_5568170 and wsnp_CAP11_c209_198467 of 5D, reported previously linked to a soil-borne wheat mosaic virus (SBWMV) resistance gene, were also associated with FHB resistance in this study. Conclusion: The syntenic FHB resistant loci and associated SNP markers identified in this study are valuable for FHB resistance breeding via marker-assisted selection.

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