Proteomics Analysis of Serum Proteins in Gestational Diabetes

Abstract Purpose The purpose of this study was to screen serum proteins for biomarkers of gestational diabetes mellitus (GDM) and to investigate its pathogenesis by analyzing the differences in serum proteomics between pregnant women with GDM and healthy pregnant women. Methods High performance liquid chromatography-mass spectrometry was used to identify differentially expressed serum proteins between pregnant women with GDM and healthy pregnant women, and bioinformatics analysis was then performed on the identified proteins. Results A total of 1152 quantifiable proteins were detected; among them, 15 were up-regulated in the serum of GDM pregnant women, while 26 were down-regulated. The subsequent parallel reaction monitoring (PRM) assay validated the expression levels of 12 out of 41 differentially expressed proteins. Moreover, bioinformatics analysis revealed that the differentially expressed proteins are involved in multiple biological processes and signaling pathways related to lipid metabolism, glycan degradation, immune response, and platelet aggregation. Conclusions This study identified 41 serum proteins with differential expression between pregnant women with GDM and healthy pregnant women, providing new candidate molecules for elucidating GDM pathogenesis and screening therapeutic targets.

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Serum proteomics analysis reveals the thermal fitness of dairy buffalo to chronic heat stress

10.21203/rs.2.13215/v1 ◽

2019 ◽

Author(s):

Liu Ping ◽

Guo Lulu ◽

Mao Huaming ◽

Gu Zhaobing

Keyword(s):

Heat Stress ◽

Oxygen Delivery ◽

Production Efficiency ◽

Serum Proteins ◽

Differentially Expressed ◽

Blood Oxygen ◽

Label Free ◽

Serum Proteomics ◽

Parallel Reaction Monitoring ◽

Dairy Buffalo

Abstract Background: Chronic heat stress (CHS), aggravated by global warming, reduces the production efficiency of the buffalo dairy industry. CHS changes protein abundance, and low-abundant proteins take important roles in biological processes. Results: The objective of the study was to assess differences in low-abundant serum proteins in dairy buffaloes at thermoneutral (TN) or under chronic heat stress (CHS) conditions with proteomic approaches. Six dairy buffaloes as reference animal raised in TN season, and another six dairy buffaloes raised in CHS to discover the molecular mechanism of thermal fitness in hot season with serum proteomics. After the removal of multiple high-abundant proteins in serum, 344 low-abundant proteins were identified in serum with label-free quantification. Of these, 17 low-abundant differentially expressed serum proteins with known functions were detected, and five of these differentially expressed proteins were validated with parallel reaction monitoring. These five proteins were associated with various aspects of heat stress, including decreased heat production, increased blood oxygen delivery, and enhanced natural disease resistance. Conclusions: Lipase (LPL), glutathione peroxidase 3 (GPX3), cathelicidin-2 (CATHL2), ceruloplasmin (CP), and hemoglobin subunit alpha 1 (HBA1) were shown to play cooperative roles in CHS fitness in dairy buffalo. Dairy buffaloes adapt to CHS and hypoxia with high levels of RBCs, HBA1 and CP increased blood oxygen delivery capacity and thermal fitness.

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Screening of differentially expressed proteins from syncytiotrophoblast for severe early-onset preeclampsia in women with gestational diabetes mellitus using tandem mass tag quantitative proteomics

BMC Pregnancy and Childbirth ◽

10.1186/s12884-018-2066-9 ◽

2018 ◽

Vol 18 (1) ◽

Cited By ~ 4

Author(s):

Xiaotong Sun ◽

Tao Qu ◽

Xiyan He ◽

Xueping Yang ◽

Nan Guo ◽

...

Keyword(s):

Diabetes Mellitus ◽

Gestational Diabetes ◽

Gestational Diabetes Mellitus ◽

Early Onset ◽

Quantitative Proteomics ◽

Differentially Expressed ◽

Tandem Mass ◽

Differentially Expressed Proteins ◽

Tandem Mass Tag ◽

Early Onset Preeclampsia

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Initial Proteomics Analysis of Differentially Expressed Proteins from Mycoplasma gallisepticum Vaccine Strains ts-11 and F Detected by Western Blotting

International Journal of Poultry Science ◽

10.3923/ijps.2006.330.336 ◽

2006 ◽

Vol 5 (4) ◽

pp. 330-336 ◽

Cited By ~ 3

Author(s):

S.D. Collier . ◽

G.T. Pharr . ◽

S.L. Branton . ◽

J.D. Evans . ◽

S.A. Leigh . ◽

...

Keyword(s):

Western Blotting ◽

Mycoplasma Gallisepticum ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

Proteomics Analysis

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Identification of differentially expressed proteins in the injured lung from zinc chloride smoke inhalation based on proteomics analysis

Respiratory Research ◽

10.1186/s12931-019-0995-0 ◽

2019 ◽

Vol 20 (1) ◽

Cited By ~ 3

Author(s):

Xiaowei Xie ◽

Jingan Zhao ◽

Lixin Xie ◽

Haiyan Wang ◽

Yan Xiao ◽

...

Keyword(s):

Zinc Chloride ◽

Differentially Expressed ◽

Smoke Inhalation ◽

Differentially Expressed Proteins ◽

Proteomics Analysis ◽

Injured Lung

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Serum proteins differentially expressed in early- and late-onset preeclampsia assessed using iTRAQ proteomics and bioinformatics analyses

PeerJ ◽

10.7717/peerj.9753 ◽

2020 ◽

Vol 8 ◽

pp. e9753

Author(s):

Chengcheng Tu ◽

Feng Tao ◽

Ying Qin ◽

Mingzhu Wu ◽

Ji Cheng ◽

...

Keyword(s):

Mass Spectrometry ◽

Lipid Metabolism ◽

Pregnant Women ◽

Serum Proteins ◽

Late Onset ◽

Differentially Expressed ◽

Coagulation Cascade ◽

Enzyme Linked Immunosorbent Assay ◽

Serum Samples ◽

Bioinformatics Analyses

Background Preeclampsia remains a serious disorder that puts at risk the lives of perinatal mothers and infants worldwide. This study assessed potential pathogenic mechanisms underlying preeclampsia by investigating differentially expressed proteins (DEPs) in the serum of patients with early-onset preeclampsia (EOPE) and late-onset preeclampsia (LOPE) compared with healthy pregnant women. Methods Blood samples were collected from four women with EOPE, four women with LOPE, and eight women with normal pregnancies, with four women providing control samples for each preeclampsia group. Serum proteins were identified by isobaric tags for relative and absolute quantitation combined with liquid chromatography–tandem mass spectrometry. Serum proteins with differences in their levels compared with control groups of at least 1.2 fold-changes and that were also statistically significantly different between the groups at P < 0.05 were further analyzed. Bioinformatics analyses, including gene ontology and Kyoto Encyclopedia of Genes and Genomes signaling pathway analyses, were used to determine the key proteins and signaling pathways associated with the development of PE and to determine those DEPs that differed between women with EOPE and those with LOPE. Key protein identified by mass spectrometry was verified by enzyme linked immunosorbent assay (ELISA). Results Compared with serum samples from healthy pregnant women, those from women with EOPE displayed 70 proteins that were differentially expressed with significance. Among them, 51 proteins were significantly upregulated and 19 proteins were significantly downregulated. In serum samples from women with LOPE, 24 DEPs were identified , with 10 proteins significantly upregulated and 14 proteins significantly downregulated compared with healthy pregnant women. Bioinformatics analyses indicated that DEPs in both the EOPE and LOPE groups were associated with abnormalities in the activation of the coagulation cascade and complement system as well as with lipid metabolism. In addition, 19 DEPs in the EOPE group were closely related to placental development or invasion of tumor cells. Downregulationof pregnancy-specific beta-1-glycoprotein 9 (PSG9) in the LOPE group was confirmed by ELISA. Conclusion The pathogenesis of EOPE and LOPE appeared to be associated with coagulation cascade activation, lipid metabolism, and complement activation. However, the pathogenesis of EOPE also involved processes associated with greater placental injury. This study provided several new proteins in the serum which may be valuable for clinical diagnosis of EOPE and LOPE, and offered potential mechanisms underpinning the development of these disorders.

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Isobaric Tags for Relative and Absolute Quantitation-Based Quantitative Serum Proteomics Analysis in Ischemic Stroke Patients With Hemorrhagic Transformation

Frontiers in Cellular Neuroscience ◽

10.3389/fncel.2021.710129 ◽

2021 ◽

Vol 15 ◽

Author(s):

Zhifeng Qi ◽

Shuhua Yuan ◽

Xixi Zhou ◽

Xunming Ji ◽

Ke Jian Liu

Keyword(s):

Ischemic Stroke ◽

Interaction Analysis ◽

Hemorrhagic Transformation ◽

Differentially Expressed ◽

Differentially Expressed Proteins ◽

Stroke Patients ◽

Absolute Quantitation ◽

Metabolic Pathway Analysis ◽

Serum Proteomics ◽

Isobaric Tags

Hemorrhagic transformation (HT), which occurs with or without reperfusion treatments (thrombolysis and/or thrombectomy), deteriorates the outcomes of ischemic stroke patients. It is essential to find clinically reliable biomarkers that can predict HT. In this study, we screened for potential serum biomarkers from an existing blood bank and database with 243 suspected acute ischemic stroke (AIS) patients. A total of 37 patients were enrolled, who were diagnosed as AIS without receiving reperfusion treatment. They were divided into two groups based on whether they were accompanied with HT or not (five HT and 32 non-HT). Serum samples were labeled by isobaric tags for relative and absolute quantitation (iTRAQ) and analyzed by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and compared under NCBInr database. A total of 647 proteins in sera samples were captured, and the levels of 17 proteins (12 upregulated and five downregulated) were significantly different. These differentially expressed proteins were further categorized with Gene Ontology functional classification annotation and Kyoto Encyclopedia of Genes and Genomes metabolic pathway analysis into biological processes. Further protein–protein interaction analysis using String database discovered that, among the differentially expressed proteins, 10 pairs of proteins were found to have crosstalk connections, which may have direct (physical) and indirect (functional) interactions for the development of HT. Our findings suggest that these differentially expressed proteins could serve as potential biomarkers for predicting HT after ischemic stroke.

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Comparative anlysis of the differential expressed proteins in serumbetween healthy and Brucellaovis infected sheep

Indian Journal of Animal Research ◽

10.18805/ijar.10978 ◽

2016 ◽

Author(s):

Guoping Wang ◽

Run Wu

Keyword(s):

Gel Electrophoresis ◽

High Performance ◽

Ion Trap ◽

Serum Proteins ◽

Differentially Expressed ◽

High Abundance ◽

Potential Candidate ◽

Material Transport ◽

Two Dimensional Gel Electrophoresis ◽

Amyloid A

Disease-related serum proteins in sheep infected with Brucellaovis were assessed by conventional two-dimensional gel electrophoresis (2-DE). High-abundance proteins in serum were depleted by Albumin & IgG Depletion SpintrapTM, with the remaining proteins retrieved using the 2-D clean-up kit. Differentially expressed proteins were detected with the PDQUest8.0 software and identified by a high performance liquid chromatography system equipped with an ion trap mass spectrometer. Both SDS-PAGE and 2-DE gel electrophoresis confirmed that high-abundance protein components were removed thoroughly by the 2-D clean-up kit and Albumin & IgG Depletion SpintrapTM. A total of 9 differentially expressed protein spots were successfully identified as haptoglobin, serum amyloid A, Ig heavy chain, Igl-1 chain, and apolipoprotein C-III, known to be involved in stress response, energy metabolism, and material transport. These findings provide a valuable clue for investigating host immune response to pathogens at the protein level; further understanding of these novel proteins identified would be helpful for finding out potential candidate targets for diagnosis and developing new vaccines for brucellosis.

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