scholarly journals Characterization of the content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds by SR-FTIR

2021 ◽  
Author(s):  
Qiang Shi ◽  
Can Chen ◽  
Muzhi Li ◽  
Yang Chen ◽  
Yan Xu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Rotator Cuff ◽  
Load Transfer ◽  
Mechanical Test ◽  
Three Dimensional ◽  
Decellularized Extracellular Matrix ◽  
Quantitative Mapping ◽  
Extracellular Matrix Molecules ◽  
Histological Morphology

Abstract Background: Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.Methods: Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcified. The decellularized book-shaped enthesis scaffolds were conducted and intrinsic ultrastructure was evaluated by histological staining and scanning electron microscopy (SEM), respectively. The content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR.Results: The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtaine©d. Histomorphology and SEM evaluated the decellularized effect and the structure of extracellular matrix during decellularization. After mechanical test, we found the failure load in the NET group was higher than that in the DEM group (P < 0.05), reached 1.32 times as much as that in the DEM group. Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis.Conclusion: SR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, it can be used for quantitative mapping of the content and distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.

scholarly journals Characterization of the Content and Distribution of Collagen and Proteoglycan in the Decellularized Book-Shaped Enthesis Scaffolds by SR-FTIR

2020 ◽  
Author(s):  
Hongbin Lu ◽  
qiang shi ◽  
Can Chen ◽  
Muzhi Li ◽  
Yang Chen ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Rotator Cuff ◽  
Load Transfer ◽  
Mechanical Test ◽  
Three Dimensional ◽  
Decellularized Extracellular Matrix ◽  
Quantitative Mapping ◽  
Extracellular Matrix Molecules ◽  
Histological Morphology

Abstract Background: Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported.Methods: Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcified. The decellularized book-shaped enthesis scaffolds were conducted and intrinsic ultrastructure was evaluated by histological staining and scanning electron microscopy (SEM), respectively. The content and distribution of collagen and proteoglycan in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR.Results: The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtained. Histomorphology and SEM evaluated the decellularized effect and the structure of extracellular matrix during decellularization. After mechanical test, we found the failure load in the NET group was higher than that in the DEM group (P < 0.05), reached 1.32 times as much as that in the DEM group. Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis.Conclusion: SR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, it can be used for quantitative mapping of the content and distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.

scholarly journals Characterization of the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds by SR-FTIR

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Qiang Shi ◽  
Can Chen ◽  
Muzhi Li ◽  
Yang Chen ◽  
Yan Xu ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Rotator Cuff ◽  
Load Transfer ◽  
Three Dimensional ◽  
Decellularized Extracellular Matrix ◽  
Quantitative Mapping ◽  
Extracellular Matrix Molecules ◽  
Histological Staining ◽  
Histological Morphology

Abstract Background Bone-tendon interface (enthesis) plays a pivotal role in relaxing load transfer between otherwise structurally and functionally distinct tissue types. Currently, decellularized extracellular matrix (DEM) from enthesis provide a natural three-dimensional scaffold with tissue-specific orientations of extracellular matrix molecules for enthesis regeneration, however, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff by SR-FTIR have not been reported. Methods Native enthesis tissues (NET) harvested from rabbit rotator cuff were sectioned into cuboid (about 30 mm × 1.2 mm × 10 mm) for decalcification. The decellularized book-shaped enthesis scaffolds and intrinsic ultrastructure were evaluated by histological staining and scanning electron microscopy (SEM), respectively. The distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were also measured innovatively by SR-FTIR. Results The decellularized book-shaped enthesis scaffolds from rabbit rotator cuff were successfully obtained. Histomorphology and SEM evaluated the effect of decellularization and the structure of extracellular matrix during decellularization. After mechanical testing, the failure load in the NET group showed significantly higher than that in the DEM group (P < 0.05). Meanwhile, the stiffness of the DEM group was significantly lower than the NET group. Furthermore, the distributions of collagen and PGs content in the decellularized book-shaped enthesis scaffolds were decreased obviously after decellularization by SR-FTIR quantitative analysis. Conclusion SR-FTIR was applied innovatively to characterize the histological morphology of native enthesis tissues from rabbit rotator cuff. Moreover, this technology can be applied for quantitative mapping of the distribution of collagen and PGs content in the decellularized book-shaped enthesis scaffolds.

A bioartificial rat heart tissue: Perfusion decellularization and characterization

2019 ◽  
Vol 42 (12) ◽  
pp. 757-764 ◽  
Author(s):  
Busra Ozlu ◽  
Mert Ergin ◽  
Sevcan Budak ◽  
Selcuk Tunali ◽  
Nuh Yildirim ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Tissue Perfusion ◽  
Heart Tissue ◽  
Cell Nuclei ◽  
Tissue Sections ◽  
The Past ◽  
Significant Difference ◽  
Structural Preservation

Despite remarkable advancement in the past decades, heart-related defects are still prone to progress irreversibly and can eventually lead to heart failure. A personalized extracellular matrix–based bioartificial heart created by allografts/xenografts emerges as an alternative as it can retain the original three-dimensional architecture combined with a preserved natural heart extracellular matrix. This study aimed at developing a procedure for decellularizing heart tissue harvested from rats and evaluating decellularization efficiency in terms of residual nuclear content and structural properties. Tissue sections showed no or little visible cell nuclei in decellularized heart, whereas the native heart showed dense cellularity. In addition, there was no significant variation in the alignment of muscle fibers upon decellularization. Furthermore, no significant difference was detected between native and decellularized hearts in terms of fiber diameter. Our findings demonstrate that fiber alignment and diameter can serve as additional parameters in the characterization of biological heart scaffolds as these provide valuable input for evaluating structural preservation of decellularized heart. The bioartificial scaffold formed here can be functionalized with patient’s own material and utilized in regenerative engineering.

scholarly journals Printing three-dimensional tissue analogues with decellularized extracellular matrix bioink

2014 ◽  
Vol 5 (1) ◽  
Author(s):  
Falguni Pati ◽  
Jinah Jang ◽  
Dong-Heon Ha ◽  
Sung Won Kim ◽  
Jong-Won Rhie ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Decellularized Extracellular Matrix

Characterization of interconnectivity of gelatin methacrylate hydrogels using photoacoustic imaging

Lab on a Chip ◽  
2022 ◽  
Author(s):  
Wenxiu Zhao ◽  
Haibo Yu ◽  
Zhixing Ge ◽  
Xiaoduo Wang ◽  
Yuzhao Zhang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Photoacoustic Imaging ◽  
Three Dimensional

Hydrogels can provide a three-dimensional microenvironment for cells and thus serve as an extracellular matrix in a biofabrication process. The properties of hydrogels, such as their porosity and mechanical properties,...

Fabrication and Characterization of P34HB Scaffolds with Oriented Microtubules

2014 ◽  
Vol 898 ◽  
pp. 318-321 ◽  
Author(s):  
Xin Hui Wang ◽  
Lin Sang ◽  
Zhi Yong Wei ◽  
Li Jie Zhai ◽  
Min Qi
Keyword(s):  
Mechanical Property ◽  
Extracellular Matrix ◽  
Pore Size ◽  
Three Dimensional ◽  
Dimensional Structure ◽  
Three Dimensional Structure ◽  
Significant Potential ◽  
Fabrication And Characterization

Sponge-like scaffold with a specific three-dimensional structure resembling the actual extracellular matrix of a particular tissue show significant potential for the regeneration and repair of damaged anisotropic tissues. In this research, an oriented microtubular P34HB scaffold was prepared successfully. The mechanical property showed that anisotropy of modulus is much greater than a typical non-oriented scaffold. Altering the P34HB concentration allowed P34HB scaffolds to be produced with complex pore orientations, and anisotropy in pore size and alignment.

Structural characterization of heparinase III from Bacteroides thetaiotaomicron

2014 ◽  
Vol 70 (a1) ◽  
pp. C480-C480
Author(s):  
Thirumalaiselvi Ulaganathan ◽  
Maia Cherney ◽  
Deqiang Yao ◽  
Guoyun Li ◽  
Lingyun Li ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Three Dimensional ◽  
Nutrient Content ◽  
Site Directed Mutagenesis ◽  
Bacteroides Thetaiotaomicron ◽  
Connective Tissues ◽  
Crystal Forms ◽  
Repeat Units ◽  
Polysaccharide Lyases

Glycosaminoglycans (GAGs) are linear polysaccharides comprised of disaccharide repeat units, a hexuronic acid, glucuronic acid (GlcA) or iduronic acid (IdoA), linked to a hexosamine, N-acetylglucosamine (GlcNAc) or N-acetylgalactosamine (GalNAc). GAGs undergo further modification such as epimerization and sulfation. These polysaccharides are abundant in the extracellular matrix and connective tissues. GAGs function in stabilization of the fibrillar extracellular matrix (ECM), control of hydration, regulation of tissue, organism development by controlling cell cycle, cell behavior, and differentiation. Niche adapted bacteria expresses enzymes called polysaccharide lyases (PL), which degrade GAGs for their nutrient content. Polysaccharide lyases have been classified into 23 sequence-related families. Comparison of three-dimensional structures of the prototypic members of these families allowed identification of distant evolutionary relationships between lyases that were unrecognized at the sequence level and identified occurrences of convergent evolution. We have characterized structurally and enzymatically Heparinase III (HepIII) from Bacteroides thetaiotaomicron, which is classified within the PL12 family. HepIII is a 72.5KDa protein. We will present the X-Ray structures of two crystal forms of HepIII of resolution 1.8 Å and 2.6 Å. HepIII contains two domains, the N-terminal α-helical domain forming a toroid and the C-terminal β-sheet domain. Comparison with recently determined structures of two other heparinases from the same PL12 family allowed us to identify structural flexibility in the arrangement of the domains indicating open-close movement. Based on comparison with other GAG lyases we identified Tyr301 as the main catalytic residue and confirmed this by site-directed mutagenesis. We have characterized substrate preference of HepIII toward sulfate poor heparan sulcate substrate.

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