Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

Abstract Background: The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle.Results: MFC assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. Firstly, an acid tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV.Conclusions: A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.

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Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

10.21203/rs.3.rs-41281/v3 ◽

2020 ◽

Author(s):

Tianwen Zheng ◽

Bin Xu ◽

Yaliang Ji ◽

Wenming Zhang ◽

Fengxue Xin ◽

...

Keyword(s):

Fuel Cell ◽

Microbial Fuel Cell ◽

Unsaturated Fatty Acids ◽

Neutral Red ◽

Transmembrane Transport ◽

Electron Shuttle ◽

Succinate Production ◽

Terminal Electron Acceptor ◽

Glycerol Utilization ◽

Acid Tolerant

Abstract Background: The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle.Results: MFC assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. Firstly, an acid tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV.Conclusions: A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.

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Microbial fuel cell-assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

Biotechnology for Biofuels ◽

10.1186/s13068-021-01882-5 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Tianwen Zheng ◽

Bin Xu ◽

Yaliang Ji ◽

Wenming Zhang ◽

Fengxue Xin ◽

...

Keyword(s):

Fuel Cell ◽

Microbial Fuel Cell ◽

Unsaturated Fatty Acids ◽

Neutral Red ◽

Transmembrane Transport ◽

Electron Shuttle ◽

Succinate Production ◽

Terminal Electron Acceptor ◽

Glycerol Utilization ◽

Acid Tolerant

Abstract Background The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid-tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle. Results MFC-assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. First, an acid-tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV. Conclusions A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.

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Microbial fuel cell assisted utilization of glycerol for succinate production by mutant of Actinobacillus succinogenes

10.21203/rs.3.rs-41281/v2 ◽

2020 ◽

Author(s):

Tianwen Zheng ◽

Bin Xu ◽

Yaliang Ji ◽

Wenming Zhang ◽

Fengxue Xin ◽

...

Keyword(s):

Fuel Cell ◽

Microbial Fuel Cell ◽

Unsaturated Fatty Acids ◽

Neutral Red ◽

Transmembrane Transport ◽

Electron Shuttle ◽

Succinate Production ◽

Terminal Electron Acceptor ◽

Glycerol Utilization ◽

Acid Tolerant

Abstract Background: The global production of glycerol is increasing year by year since the demands of biodiesel is rising. It is benefit for high-yield succinate synthesis due to its high reducing property. A. succinogenes, a succinate-producing candidate, cannot grow on glycerol anaerobically, as it needs a terminal electron acceptor to maintain the balance of intracellular NADH and NAD+. Microbial fuel cell (MFC) has been widely used to release extra intracellular electrons. However, A. succinogenes is a non-electroactive strain which need the support of electron shuttle in MFC, and pervious research showed that acid tolerant A. succinogenes has higher content of unsaturated fatty acids, which may be beneficial for the transmembrane transport of lipophilic electron shuttle.Results: MFC assisted succinate production was evaluated using neutral red as an electron shuttle to recover the glycerol utilization. Firstly, an acid tolerant mutant JF1315 was selected by atmospheric and room temperature plasma (ARTP) mutagenesis aiming to improve transmembrane transport of neutral red (NR). Additionally, MFC was established to increase the ratio of oxidized NR to reduced NR. By combining these two strategies, ability of JF1315 for glycerol utilization was significantly enhanced, and 23.92 g/L succinate was accumulated with a yield of 0.88 g/g from around 30 g/L initial glycerol, along with an output voltage above 300 mV.Conclusions: A novel MFC-assisted system was established to improve glycerol utilization by A. succinogenes for succinate and electricity production, making this system as a platform for chemicals production and electrical supply simultaneously.

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Improved Neomycin Sulfate Potency in Streptomyces fradiae Using Atmospheric and Room Temperature Plasma (ARTP) Mutagenesis and Fermentation Medium Optimization

Microorganisms ◽

10.3390/microorganisms10010094 ◽

2022 ◽

Vol 10 (1) ◽

pp. 94

Author(s):

Fei Yu ◽

Min Zhang ◽

Junfeng Sun ◽

Fang Wang ◽

Xiangfei Li ◽

...

Keyword(s):

High Throughput Screening ◽

Room Temperature ◽

Screening Method ◽

Fermentation Medium ◽

Soluble Starch ◽

High Yield ◽

Streptomyces Fradiae ◽

Temperature Plasma ◽

Artp Mutagenesis ◽

Neomycin Sulfate

To improve the screening efficiency of high-yield neomycin sulfate (NM) Streptomyces fradiae strains after mutagenesis, a high-throughput screening method using streptomycin resistance prescreening (8 μg/mL) and a 24-deep well plates/microplate reader (trypan blue spectrophotometry) rescreening strategy was developed. Using this approach, we identified a high-producing NM mutant strain, Sf6-2, via six rounds of atmospheric and room temperature plasma (ARTP) mutagenesis and screening. The mutant displayed a NM potency of 7780 ± 110 U/mL and remarkably stable genetic properties over six generations. Furthermore, the key components (soluble starch, peptone, and (NH4)2SO4) affecting NM potency in fermentation medium were selected using Plackett-Burman and optimized by Box-Behnken designs. Finally, the NM potency of Sf6-2 was increased to 10,849 ± 141 U/mL at the optimal concentration of each factor (73.98 g/L, 9.23 g/L, and 5.99 g/L, respectively), and it exhibited about a 40% and 100% enhancement when compared with before optimization conditions and the wild-type strain, respectively. In this study, we provide a new S. fradiae NM production strategy and generate valuable insights for the breeding and screening of other microorganisms.

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ARTP Mutagenesis of Schizochytrium sp. PKU#Mn4 and Clethodim-Based Mutant Screening for Enhanced Docosahexaenoic Acid Accumulation

Marine Drugs ◽

10.3390/md19100564 ◽

2021 ◽

Vol 19 (10) ◽

pp. 564

Author(s):

Lu Liu ◽

Mohan Bai ◽

Sai Zhang ◽

Jiantao Li ◽

Xianhua Liu ◽

...

Keyword(s):

Docosahexaenoic Acid ◽

Large Scale ◽

High Yield ◽

Scale Production ◽

Temperature Plasma ◽

Medium Components ◽

Yield Production ◽

Large Scale Production ◽

Acid Accumulation ◽

Artp Mutagenesis

Schizochytrium species are one of the best oleaginous thraustochytrids for high-yield production of docosahexaenoic acid (DHA, 22:6). However, the DHA yields from most wild-type (WT) strains of Schizochytrium are unsatisfactory for large-scale production. In this study, we applied the atmospheric and room-temperature plasma (ARTP) tool to obtain the mutant library of a previously isolated strain of Schizochytrium (i.e., PKU#Mn4). Two rounds of ARTP mutagenesis coupled with the acetyl-CoA carboxylase (ACCase) inhibitor (clethodim)-based screening yielded the mutant A78 that not only displayed better growth, glucose uptake and ACCase activity, but also increased (54.1%) DHA content than that of the WT strain. Subsequent optimization of medium components and supplementation improved the DHA content by 75.5% and 37.2%, respectively, compared with that of mutant A78 cultivated in the unoptimized medium. Interestingly, the ACCase activity of mutant A78 in a medium supplemented with biotin, citric acid or sodium citrate was significantly greater than that in a medium without supplementation. This study provides an effective bioengineering approach for improving the DHA accumulation in oleaginous microbes.

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Microbial fuel cells: Devices for real wastewater treatment, rather than electricity production

The Science of The Total Environment ◽

10.1016/j.scitotenv.2021.145904 ◽

2021 ◽

Vol 775 ◽

pp. 145904

Author(s):

Jaecheul Yu ◽

Younghyun Park ◽

Evy Widyaningsih ◽

Sunah Kim ◽

Younggy Kim ◽

...

Keyword(s):

Wastewater Treatment ◽

Fuel Cells ◽

Microbial Fuel Cells ◽

Electricity Production ◽

Real Wastewater

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Characterizing the Anoxic Phenotype of Pseudomonas putida Using a Bioelectrochemical System

Methods and Protocols ◽

10.3390/mps2020026 ◽

2019 ◽

Vol 2 (2) ◽

pp. 26 ◽

Cited By ~ 4

Author(s):

Bin Lai ◽

Anh Nguyen ◽

Jens Krömer

Keyword(s):

Pseudomonas Putida ◽

High Yield ◽

Gas Transfer ◽

Bioelectrochemical System ◽

Terminal Electron Acceptor ◽

Industrial Fermentation ◽

Electrochemical Technology ◽

Poor Understanding ◽

Cultivation Technology ◽

Obligate Aerobe

Industrial fermentation in aerobic processes is plagued by high costs due to gas transfer limitations and substrate oxidation to CO2. It has been a longstanding challenge to engineer an obligate aerobe organism, such as Pseudomonas putida, into an anaerobe to facilitate its industrial application. However, the progress in this field is limited, due to the poor understanding of the constraints restricting its anoxic phenotype. In this paper, we provide a methodological description of a novel cultivation technology for P. putida under anaerobic conditions, using the so-called microbial electrochemical technology within a bioelectrochemical system. By using an electrode as the terminal electron acceptor (mediated via redox chemicals), glucose catabolism could be activated without oxygen present. This (i) provides an anoxic-producing platform for sugar acid production at high yield and (ii) more importantly, enables systematic and quantitative characterizations of the phenotype of P. putida in the absence of molecular oxygen. This unique electrode-based cultivation approach offers a tool to understand and in turn engineer the anoxic phenotype of P. putida and possibly also other obligate aerobes.

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