scholarly journals Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

2020 ◽  
Author(s):  
Jingxiu Ye ◽  
Haidong Liu ◽  
Zhi Zhao ◽  
Liang Xu ◽  
Kaixiang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Chlorophyll Content ◽  
Fine Mapping ◽  
Agronomic Traits ◽  
Economic Value ◽  
Chlorophyll Synthesis ◽  
Brassica Napus L ◽  
Oil Crops ◽  
Initial Location ◽  
Dh Lines

Abstract Background: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits.Results: The cqSPDA2 locus was mapped to an interval of 21.87-22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these indel primers. Among the indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis. Conclusions: The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89-22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found.

scholarly journals Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

2020 ◽  
Vol 20 (1) ◽  
Author(s):  
Jingxiu Ye ◽  
Haidong Liu ◽  
Zhi Zhao ◽  
Liang Xu ◽  
Kaixiang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Chlorophyll Content ◽  
Fine Mapping ◽  
Agronomic Traits ◽  
Economic Value ◽  
Chlorophyll Synthesis ◽  
Brassica Napus L ◽  
Oil Crops ◽  
Initial Location ◽  
Dh Lines

Abstract Background Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus, there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. Results The cqSPDA2 locus was mapped to an interval of 21.87–22.91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2, we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these indel primers. Among these indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval, and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis. Conclusions The locus cqSPDA2, a dominant QTL for chlorophyll content in Brassica napus, was fine-mapped to a 21.89–22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) that may be responsible for chlorophyll synthesis were found.

scholarly journals Fine mapping of the QTL cqSPDA2 for chlorophyll content in Brassica napus L.

2020 ◽  
Author(s):  
Jingxiu Ye ◽  
Haidong Liu ◽  
Zhi Zhao ◽  
Liang Xu ◽  
Kaixiang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Chlorophyll Content ◽  
Fine Mapping ◽  
Agronomic Traits ◽  
Economic Value ◽  
Chlorophyll Synthesis ◽  
Brassica Napus L ◽  
Oil Crops ◽  
Initial Location ◽  
Dh Lines

Abstract Background Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Breeding Brassica napus for high light efficiency by improving photosynthetic efficiency has considerable social and economic value. In Brassica napus , there have been studies of the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We constructed near-isogenic lines (NIL), fine-mapped a chlorophyll locus, and evaluated the effect of this dominant locus on agronomic traits. Results The cqSPDA2 locus was mapped to an interval of 21.87-22. 91 Mb on the chromosome A02 of Brassica napus using doubled haploid (DH) lines. To fine-map cqSPDA2 , we built NIL and designed Indel primers covering the mapping interval. The 469 individuals in the BC 3 F 2 population were analyzed using these indel primers. Among the indel primers, 15 could narrow the mapping interval to 188 kb between Indel3 and Indel15. Next, 16 indel primers and 19 SSR primers were designed within the new narrower mapping interval , and 5 of the primer-amplified fragments were found to be polymorphic and tightly linked to the cqSPDA2 locus in the BC 4 F 2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. By gene expression analysis, we found three annotated genes in the mapping interval, including BnaA02g30260D , BnaA02g30290D and BnaA02g30310D , which may be responsible for chlorophyll synthesis. Conclusions The locus cqSPDA2 , a dominant QTL for chlorophyll content in Brassica napus , was fine-mapped to a 21.89-22.04 Mb interval on A02 . Three annotated genes ( BnaA02g30260D , BnaA02g30290D and BnaA02g30310D ) that may be responsible for chlorophyll synthesis were found.

scholarly journals Fine Mapping of the QTL cqSPDA2 for Chlorophyll Content in Brassica Napus L.

2020 ◽  
Author(s):  
Jingxiu Ye ◽  
Haidong Liu ◽  
Zhi Zhao ◽  
Liang Xu ◽  
Kaixiang Li ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Chlorophyll Content ◽  
Fine Mapping ◽  
Agronomic Traits ◽  
Light Condition ◽  
Chlorophyll Synthesis ◽  
Tibet Plateau ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Initial Location

Abstract Background: Chlorophyll is the most important factor enabling plants to absorb, transfer and transform light energy and plays an important role in yield formation. Brassica napus is one of the most important oil crops. Qinghai Province is located in the Qinghai-Tibet Plateau in China. Solar radiation and its intensity are higher, and the light duration is longer. Under this light condition, conducting high light efficiency breeding of Brassica napus from the perspective of improving photosynthetic efficiency has considerable social and economic value. At present, the fine mapping of chlorophyll quantitative trait loci (QTLs) in many crops has been studied, especially in rice. In Brassica napus, there have been studies on the initial location of chlorophyll in seed embryos and pericarps, but there are few reports on the fine mapping of chlorophyll QTLs. We built near-isogenic lines, fine mapped the chlorophyll locus, and evaluated the effect of the dominant locus on agronomic traits.Results: The cqSPDA2 locus was mapped in an interval of 21.87-22.91 Mb on the A02 chromosome of Brassica napus using doubled haploid (DH) lines. To fine map cqSPDA2, we built near-isogenic lines and designed Indel primers covering the mapping interval. The 469 individuals in the BC3F2 population were analyzed using these Indel primers. Among the Indel primers, 15 narrowed the mapping interval to 5.2 cM between Indel3 and Indel15. Next, 16 Indel primers and 19 SSR primers were designed within the new narrow mapping interval, and 7 primers were polymorphic and tightly linked to the cqSPDA2 locus in the BC4F2 population. The mapping interval was narrowed to 152 kb on A02 between SSR2 and Indel15. We found three annotated genes in the mapping interval, including BnaA02g30260D, BnaA02g30290D and BnaA02g30310D, which may be responsible for chlorophyll synthesis by gene expression analysis. Conclusions: The locus cqSPDA2, the dominant QTL for chlorophyll content in Brassica napus, was fine mapped to the 21.89-22.04 Mb interval on A02. Three annotated genes (BnaA02g30260D, BnaA02g30290D and BnaA02g30310D) which may be responsible for chlorophyll synthesis were found.

Genes and Quantitative Trait Loci Mapping for Major Agronomic Traits in Brassica napus L.

2018 ◽  
pp. 41-85 ◽  
Author(s):  
Régine Delourme ◽  
Anne Laperche ◽  
Anne-Sophie Bouchet ◽  
Mélanie Jubault ◽  
Sophie Paillard ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Quantitative Trait Loci ◽  
Quantitative Trait ◽  
Agronomic Traits ◽  
Quantitative Trait Loci Mapping ◽  
Brassica Napus L ◽  
Trait Loci

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population

scholarly journals Fine-mapping and transcriptome analysis of a candidate gene controlling plant height in Brassica napus L.

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Xiaodong Wang ◽  
Ming Zheng ◽  
Hongfang Liu ◽  
Liang Zhang ◽  
Feng Chen ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Candidate Gene ◽  
Plant Height ◽  
Fine Mapping ◽  
Transcriptome Analysis ◽  
Brassica Napus L

QTL analysis of yield-related traits and their association with functional markers in Brassica napus L.

2007 ◽  
Vol 58 (8) ◽  
pp. 759 ◽  
Author(s):  
Yuanyuan Li ◽  
Jinxiong Shen ◽  
Tonghua Wang ◽  
Qingfang Chen ◽  
Xingguo Zhang ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Agronomic Traits ◽  
High Yield ◽  
Functional Markers ◽  
Multiple Traits ◽  
Brassica Napus L ◽  
Breeding Programs ◽  
F2 Population ◽  
Expressed Sequence ◽  
Simple Sequence

Yield is one of the most important traits in Brassica napus breeding programs. Quantitative trait loci (QTLs) for yield-related traits based on genetic mapping would help breeders to develop high-yield cultivars. In this study, a genetic linkage map of B. napus, containing 142 sequence-related amplified polymorphism (SRAP) markers, 163 functional markers, 160 simple sequence repeat (SSR) markers, and 117 amplified fragment length polymorphism (AFLP) markers, was constructed in an F2 population of 184 individuals resulting from the cross SI-1300 × Eagle. This map covered 2054.51 cM with an average marker interval of 3.53 cM. Subsequently, QTLs were detected for 12 yield-related traits in Wuhan and Jingmen. In total, 133 QTLs were identified, including 14 consistent ones across the 2 locations. Fifteen of 20 linkage groups (LGs) were found to have QTLs for the 12 traits investigated, and most of the QTLs were clustered, especially on LGs N2 and N7, where similar QTL positions were identified for multiple traits. Eight of 10 QTLs for yield per plant (YP) were also associated with number of seeds per silique (SS), number of siliques per plant (SP), and/or 1000-seed weight (SW). In addition, 45 functional markers involved in 39 expressed sequence tags (ESTs) were linked to the QTLs of 12 traits. The present results may serve as a valuable basis for further molecular dissection of agronomic traits in B. napus, and the markers related to QTLs may offer promising possible makers for marker assisted selection.

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