scholarly journals Bioconversion of poly-γ-glutamic acid (γ-PGA) from fulvic acid powder produced from the wastewater of yeast molasses fermentation

2020 ◽  
Author(s):  
Yazhou Li ◽  
Jianghan Wang ◽  
Luxin Ke ◽  
Xiuyun Zhao ◽  
Gaofu Qi
Keyword(s):  
Fulvic Acid ◽  
Low Cost ◽  
Value Added ◽  
Fermentation Medium ◽  
Systemic Resistance ◽  
Callose Deposition ◽  
Wastewater Disposal ◽  
Industry Waste ◽  
A Value ◽  
Ethylene Signalling

Abstract Background: Molasses is a wildly used feedstock for fermentation, but it poses a severe wastewater-disposal problem worldwide. Recently, the wastewater produced by yeast during molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied in soil. In this study, the low-cost FA powder was bioconverted into a value-added product, γ-PGA, by a glutamate independent producer, Bacillus velezensis GJ11. Results: With FA powder, the substrates of sodium glutamate and citrate sodium used in medium were decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium. In the optimized FA powder fermentation medium, the γ-PGA was produced with its maximum concentration at 42.55 g/L and a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolyzates was dependent on the ethylene signalling and NPR1. Conclusions: To our knowledge, this is the first report of using the industry waste, FA powder, as a sustainable substrate for the microbial synthesis of γ-PGA. This bioprocess can not only develop a new way of FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

scholarly journals Microbial synthesis  of poly- γ -glutamic acid ( γ -PGA) with  fulvic acid powder, the waste from  yeast molasses fermentation

2020 ◽  
Author(s):  
Yazhou Li ◽  
Jianghan Wang ◽  
Na Liu ◽  
Luxin Ke ◽  
Xiuyun Zhao ◽  
...  
Keyword(s):  
Fulvic Acid ◽  
Low Cost ◽  
Value Added ◽  
Fermentation Medium ◽  
Systemic Resistance ◽  
Sodium Glutamate ◽  
Microbial Synthesis ◽  
Callose Deposition ◽  
Wastewater Disposal ◽  
Industry Waste

Abstract Background: Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate independent producer of Bacillus velezensis GJ11.Results: FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+ and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g. H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signalling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions: To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

scholarly journals Microbial synthesis of poly-γ-glutamic acid (γ-PGA) with fulvic acid powder, the waste from yeast molasses fermentation

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Yazhou Li ◽  
Jianghan Wang ◽  
Na Liu ◽  
Luxin Ke ◽  
Xiuyun Zhao ◽  
...  
Keyword(s):  
Fulvic Acid ◽  
Low Cost ◽  
Value Added ◽  
Fermentation Medium ◽  
Systemic Resistance ◽  
Sodium Glutamate ◽  
Microbial Synthesis ◽  
Callose Deposition ◽  
Wastewater Disposal ◽  
Industry Waste

Abstract Background Molasses is a wildly used feedstock for fermentation, but it also poses a severe wastewater-disposal problem worldwide. Recently, the wastewater from yeast molasses fermentation is being processed into fulvic acid (FA) powder as a fertilizer for crops, but it consequently induces a problem of soil acidification after being directly applied into soil. In this study, the low-cost FA powder was bioconverted into a value-added product of γ-PGA by a glutamate-independent producer of Bacillus velezensis GJ11. Results FA power could partially substitute the high-cost substrates such as sodium glutamate and citrate sodium for producing γ-PGA. With FA powder in the fermentation medium, the amount of sodium glutamate and citrate sodium used for producing γ-PGA were both decreased around one-third. Moreover, FA powder could completely substitute Mg2+, Mn2+, Ca2+, and Fe3+ in the fermentation medium for producing γ-PGA. In the optimized medium with FA powder, the γ-PGA was produced at 42.55 g/L with a productivity of 1.15 g/(L·h), while only 2.87 g/L was produced in the medium without FA powder. Hydrolyzed γ-PGA could trigger induced systemic resistance (ISR), e.g., H2O2 accumulation and callose deposition, against the pathogen’s infection in plants. Further investigations found that the ISR triggered by γ-PGA hydrolysates was dependent on the ethylene (ET) signaling and nonexpressor of pathogenesis-related proteins 1 (NPR1). Conclusions To our knowledge, this is the first report to use the industry waste, FA powder, as a sustainable substrate for microbial synthesis of γ-PGA. This bioprocess can not only develop a new way to use FA powder as a cheap feedstock for producing γ-PGA, but also help to reduce pollution from the wastewater of yeast molasses fermentation.

Rice Husk-Derived Mesoporous Silica Nanostructure for Supercapacitors Application: A Possible Approach for Recycling Bio-Waste into a Value-Added Product

2021 ◽  
Author(s):  
P. Araichimani ◽  
K.M. Prabu ◽  
G. Suresh Kumar ◽  
Gopalu Karunakaran ◽  
S. Surendhiran ◽  
...  
Keyword(s):  
Rice Husk ◽  
Redox Reactions ◽  
Low Cost ◽  
Combustion Process ◽  
Value Added ◽  
Mesoporous Structure ◽  
Rice Husks ◽  
A Value ◽  
Current Densities ◽  
Thermal Combustion

Abstract We synthesized mesoporous SiO2 nanomatrix using rice husks as a precursor through a facile thermal combustion process. XRD, FTIR, EDX, and TEM analyses were used to validate the produced mesoporous SiO2 nanomatrix. Electrochemical measurements were used to determine the specific capacitance of mesoporous SiO2 nanomatrix, and the results showed that the specific capacitances are 216, 204, 182, 163, 152, 142, 135, 133, 124.4, 124 F/g at current densities of 0.5, 1, 2, 4, 6, 8, 10, 12, 14, and 16 A/g. The benefit of impurities, as well as the large surface area and mesoporous structure of rice husk derived SiO2 nanostructures, allow Faradaic redox reactions at the electrode surface and the resulting supercapacitive behavior. This research might lead to a low-cost technique of producing supercapacitor electrodes using rice husk-derived SiO2 as a precursor.

scholarly journals Soro de Queijo para Produção de β-Galactosidase

UNICIÊNCIAS ◽  
2019 ◽  
Vol 23 (1) ◽  
pp. 31
Author(s):  
Alessandra Bosso ◽  
Adriana Aparecida Bosso Tomal ◽  
Josemeyre Bonifácio Da Silva ◽  
Hélio Hiroshi Suguimoto
Keyword(s):  
Dairy Products ◽  
Cheese Whey ◽  
Value Added ◽  
Fermentation Medium ◽  
Nutritional Composition ◽  
World Population ◽  
Surplus Production ◽  
A Value ◽  
De Se ◽  
Main Component

Aproximadamente 75 % da população mundial apresenta intolerância à lactose, doença congênita caracterizada pela incapacidade de absorver o açúcar presente no leite e seus derivados lácteos. Esta incapacidade se deve basicamente a inatividade ou baixa atividade da enzima intestinal β-galactosidase. Até o presente momento, o tratamento para as pessoas intolerantes à lactose inclui evitar o consumo de leite ou fazer uso de leite e produtos lácteos com ausência ou teor reduzido de lactose além do uso da enzima lactase já disponível comercialmente. A enzima β-galactosidase é responsável pela hidrólise das ligações galactosídicas da lactose em seus monossacarídeos, glicose e galactose. É produzida principalmente em microrganismos incluindo fungos filamentosos, bactérias e leveduras. Um dos principais requisitos para produção da enzima a partir de microrganismo é determinar a melhor composição nutricional do meio de fermentação. O soro de queijo é fonte abundante de carbono tendo a lactose como seu principal componente e, portanto, pode ser usado por microrganismos, como meio de cultivo para produção de β-galactosidase. O uso do soro de queijo com esta finalidade pode contribuir para diminuição do impacto econômico e ambiental causado pela sua produção excedente além de, se tornar um produto com valor agregado. Palavras-chave: Intolerância à Lactose. Soro de Queijo. Fermentação. Microrganismos. Abstract75% of the world population is lactose intolerant, a congenital disease characterized by an inability to absorb the sugar present in milk and its dairy products. This inability is basically due to inactivity or low activity of the intestinal β-galactosidase enzyme. The treatment includes avoiding the consumption of milk or making use of milk and dairy products lacking or reduced lactose content. The enzyme β-galactosidase is responsible for the hydrolysis of the galactosidic bonds of lactose in glucose and galactose. It is found mainly in microorganisms including fungi, bacteria and yeast. One of the main requirements for the production of the enzyme from microorganism is to determine the best nutritional composition of the fermentation medium. Cheese whey is an abundant source of carbon having lactose as its main component and therefore can be used as a medium for the cultivation of microorganisms for the production of β-galactosidase. The use of cheese whey for the production of β-galactosidase may contribute to the reduction of the economic and environmental impact caused by its surplus production, in addition to becoming a value-added product. Keywords: Lactose Intolerance. Cheese Whey. Fermentation. Microorganism.

Novel CO2-Thermic Oxidation Process with Mg-Based Intermetallic Compounds and Its Application to Energy Storage Materials

2018 ◽  
Author(s):  
Younghwan Cha ◽  
Jung-In Lee ◽  
Panpan Dong ◽  
Xiahui Zhang ◽  
Min-Kyu Song
Keyword(s):  
Intermetallic Compounds ◽  
Oxidation Process ◽  
Value Added ◽  
Energy Storage Materials ◽  
Reaction Products ◽  
Materials Synthesis ◽  
Energy Materials ◽  
A Value ◽  
Carbon Coated ◽  
Novel Strategy

A novel strategy for the oxidation of Mg-based intermetallic compounds using CO<sub>2</sub> as an oxidizing agent was realized via simple thermal treatment, called ‘CO2-thermic Oxidation Process (CO-OP)’. Furthermore, as a value-added application, electrochemical properties of one of the reaction products (carbon-coated macroporous silicon) was evaluated. Considering the facile tunability of the chemical/physical properties of Mg-based intermetallics, we believe that this route can provide a simple and versatile platform for functional energy materials synthesis as well as CO<sub>2</sub> chemical utilization in an environment-friendly and sustainable way.

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