scholarly journals Clinic-pathological Features of Epstein-barr Virus Infection, Microsatellite Instability, Tumor Mutation Burden and PD-L1 Status in 2504 Chinese Gastric Cancer Patients

2020 ◽  
Author(s):  
Li Zhang ◽  
Aiwen Wu ◽  
Zhongwu Li
Keyword(s):  
Gastric Cancer ◽  
Next Generation Sequencing ◽  
Microsatellite Instability ◽  
Cancer Patients ◽  
Epstein Barr Virus Infection ◽  
Next Generation ◽  
Barr Virus ◽  
Negative Case ◽  
Gastric Cancer Patients ◽  
Generation Sequencing

Abstract Objectives: Gastric cancer is the 4th most common cancer worldwide. Different subtype showed unique molecular features that could potentially guide therapeutic decisions. The aim of this study was to investigate the Epstein-Barr virus infection, microsatellite instability status, PD-L1 expression and gene mutation in surgically treated gastric cancer patients. Methods: We reviewed all GC patients who underwent potentially curative gastroectomy with lymphadenectomy at Peking University Cancer Hospital between 2013 and 2018 from a prospective collected medical database. We analyzed the clinic-pathological factors associated with immunohistochemistry profiles. We also analyzed gene changes through next-generation sequencing. Results: d-MMR gastric cancer patients are more likely to expression programmed death-ligand 1 (p<0.001, programmed death-ligand 1 cut off value 1%). EBV-positive and d-MMR patients were identified in 4% and 7.5% of the 2504 gastric cancer patients, respectively. The MLH1/PMS2 negative case number was 126. The MSH2/MSH6 negative case number was 14. d-MMR status was related to diffuse/mixed group (p<0.05), but not related to tumor differentiation. In our study, the microsatellite instability results detected by next generation sequencing and d-MMR gastric cancer results detected by immunohistochemistry were in high consistency. d-MMR gastric cancer patients had more microsatellite instability core. Many pathogenic genes were detected in microsatellite instability gastric cancer patients, such as POLE, ETV6, TP53, BRCA, RNF43 and other genes. Conclusion: Through immunohistochemistry and next generation sequencing, we got MSI status, protein expression, TMB and gene changes of GC, which provided a theoretical basis for future G clinical treatment.

Detection of genes related to peritoneal metastasis of primary gastric cancer based on large-panel Next Generation Sequencing.

2021 ◽  
Vol 39 (15_suppl) ◽  
pp. e16026-e16026
Author(s):  
Si Tao ◽  
Xin Liu ◽  
Chunhui Jin ◽  
Rongrong Chen
Keyword(s):  
Gastric Cancer ◽  
Next Generation Sequencing ◽  
Cancer Patients ◽  
Peritoneal Metastasis ◽  
Genetic Alterations ◽  
Next Generation ◽  
Large Panel ◽  
Before And After ◽  
Gastric Cancer Patients ◽  
Generation Sequencing

e16026 Background: Peritoneum is a common site of recurrence and metastasis of gastric cancer. The traditional clinical methods have poor sensitivity for the diagnosis of peritoneal metastasis, and there is no effective marker for predicting peritoneal metastasis of gastric cancer. In this study, the genetic profiles of primary tumors in gastric cancer patients with and without peritoneal metastasis, as well as the genetic alterations before and after metastasis in the same patient were analyzed to explore the genetic alterations associated with peritoneal metastasis. Methods: 232 patients with stage Ⅳ gastric cancer were divided into PM group and non-PM group according to whether there was peritoneal metastasis, DNA based Next Generation Sequencing (NGS) with a 1021 gene panel was performed on their samples. Clinical information was obtained synchronously from physicians and surgeons. Results: There was no significant difference in baseline clinical characteristics between patients in PM and non-PM groups. Molecular analysis revealed that in the PM group, the incidence of CDH1, CDH23, HDAC1, IDH1, KRAS, MED12, RHOA, NOTCH2, TGFBR1 mutations was significantly higher in PM group than that in non-PM group, and these mutated genes were mostly located in wnt/β- catenin and MAPK signaling pathway. On the contrary, the incidence of ERBB2 and CCNE1 amplification was significantly higher in non-PM group than in the other group. Further analysis of samples before and after peritoneal metastasis of 13 patients did not find significant differences in genetic alterations. Conclusions: Compared with other sites, the primary tumor of gastric cancer patients with peritoneal metastasis seems to have its unique molecular characteristics, and large-panel NGS can help us identify this part of patients. Future research may need to clarify whether more radical treatment strategies are needed for these patients.

scholarly journals CDH1 mutations in gastric cancer patients from northern Brazil identified by Next- Generation Sequencing (NGS)

2016 ◽  
Vol 39 (2) ◽  
pp. 189-198 ◽  
Author(s):  
Antonette El-Husny ◽  
Milene Raiol-Moraes ◽  
Marcos Amador ◽  
André M. Ribeiro-dos-Santos ◽  
André Montagnini ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Next Generation Sequencing ◽  
Cancer Patients ◽  
Next Generation ◽  
Northern Brazil ◽  
Gastric Cancer Patients ◽  
Next Generation Sequencing Ngs ◽  
Generation Sequencing

scholarly journals Metagenomic Next-Generation Sequencing for the Diagnosis of Epstein-Barr virus Pleurisy: A Case Report

2021 ◽  
Author(s):  
Hui Lv ◽  
Jin Liu ◽  
Ruoyu Zhang
Keyword(s):  
Next Generation Sequencing ◽  
Epstein Barr Virus ◽  
Effective Means ◽  
Epstein Barr Virus Infection ◽  
Next Generation ◽  
Barr Virus ◽  
Microbial Infections ◽  
Epstein Barr ◽  
Barr Virus Infection ◽  
Generation Sequencing

Chronic active Epstein-Barr virus infection (CAEBV) progresses rapidly in the later stage and has a poor prognosis. The treatments of CAEBV have no unified standard and a bad effect. Only few person could cure. It is important to diagnosis early. Metagenomic next-generation sequencing (mNGS) offers an effective means for the diagnosis of difficult, critical and rare pathogenic microbial infections. Here, we report a case of viral pleurisy caused by CAEBV identified by mNGS in an 88-year-old Chinese male patient. 

Abstract 4621: Validation and calibration of next-generation sequencing to identify Epstein-Barr Virus-positive gastric cancer

2015 ◽  
Author(s):  
M. Constanza Camargo ◽  
Reanne Bowlby ◽  
Andy Chu ◽  
Chandra S. Pedamallu ◽  
Vesteinn Thorsson ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Next Generation Sequencing ◽  
Epstein Barr Virus ◽  
Next Generation ◽  
Barr Virus ◽  
Epstein Barr ◽  
Generation Sequencing

scholarly journals Detection of activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted next-generation sequencing: a pilot study

2021 ◽  
Vol 33 (1) ◽  
Author(s):  
Neemat Kassem ◽  
Hebatallah Kassem ◽  
Loay Kassem ◽  
Mohamed Hassan
Keyword(s):  
Breast Cancer ◽  
Next Generation Sequencing ◽  
Microsatellite Instability ◽  
Cancer Patients ◽  
Liquid Biopsy ◽  
Missense Mutations ◽  
Next Generation ◽  
Breast Cancer Patients ◽  
Activating Mutations ◽  
Generation Sequencing

Abstract Background Breast cancer (BC) is the 2nd most prevalent malignancy worldwide and is the most prevalent cancer among Egyptian women. The number of newly described cancer-associated genes has grown exponentially since the emergence of next-generation sequencing (NGS) technology. We aim to identify activating mutations in liquid biopsy of Egyptian breast cancer patients using targeted NGS technology. We also demonstrate the microsatellite instability (MSI) status using BAT25, BAT26, and NR27 markers which are tested on the Bioanalyzer 2100 system. Results Twenty-one variants were detected in 15 genes: 7 Substitution-Missense, 12 Substitution-coding silent, and 2 Substitution-intronic. Regarding ClinVar database, out of 21 variants there were 14 benign variants, 3 variants with conflicting interpretations of pathogenicity, 3 variants not reported, and 1 drug response variant. TP53 p.(Pro72Arg) missense mutations were found in 75% of patients. PIK3CA p.(Ile391Met), KDR p.(Gln472His) missense mutations were detected in 25% of patients each. Two patients revealed APC gene missense mutation with p.(Ile1307Lys) and p.(Glu1317Gln) variants. Only one patient showed ATM p.(Phe858Leu) gene mutation and one showed FGFR3 p.(Ala719Thr) variant. Regarding microsatellite instability (MSI) status, 2/8 (25%) patients were MSS, 3/8 (37.5%) patients were MSI-L, and 3/8 (37.5%) patients were MSI-HI. Conclusion It is essential to use and validate minimally invasive liquid biopsy for activating mutations detection by next-generation sequencing especially in patients with inoperable disease or bone metastasis. This work should be extended with larger patient series with comparison of genetic mutations in liquid-based versus tissue-based biopsy and longer follow up period.

scholarly journals Occurrence of Helicobacter pyloriand Epstein-Barr virus infection in endoscopic and gastric cancer patients from Northern Brazil

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Carolina Rosal Teixeira de Souza ◽  
Kátia Soares de Oliveira ◽  
Jefferson José Sodré Ferraz ◽  
Mariana Ferreira Leal ◽  
Danielle Queiroz Calcagno ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Virus Infection ◽  
Cancer Patients ◽  
Epstein Barr Virus ◽  
Epstein Barr Virus Infection ◽  
Barr Virus ◽  
Northern Brazil ◽  
Epstein Barr ◽  
Barr Virus Infection ◽  
Gastric Cancer Patients
Sign in / Sign up

Export Citation Format

Share Document