scholarly journals LncRNA RHPN1-AS1 promotes cholangiocarcinoma progression and predicts poor clinical outcome through miR-345-5p/YAP1 axis

2020 ◽  
Author(s):  
Lifeng Ma ◽  
Tao Li ◽  
Guochao Liu ◽  
Jianlong Wang ◽  
Zhaoqiang Yin ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Clinical Practice ◽  
Clinical Outcome ◽  
Carcinoma Cell ◽  
Poor Clinical Outcome ◽  
Xenograft Growth ◽  
Poor Outcome ◽  
New Strategies

Abstract Background LncRNAs have proven to be involved in the initiation and progression of cholangiocarcinoma (CCA), although the mechanism by which this occurs remains unknown. Methods The current study reveals that RHPN1-AS1 was overexpressed in CCA patient samples, which predicted poor outcome of CCA patients. RHPN1-AS1 increased in vitro pancreatic carcinoma cell proliferation as well as promoted xenograft growth in vivo. Mechanistically, DANCR upregulated expression of YAP1 by competitively binding to miR-345-5p. Importantly, RHPN1-AS1 level was positively correlated with YAP1 expression level in CCA tissues. Moreover, YAP1 overexpression could predicted a poor outcome of CCA patients. Results Taken together, our results suggested that RHPN1-AS1 might be a remarkable biomarker to evaluate prognosis in CCA. Conclusion The RHPN1-AS1/YAP1 axis may provide new strategies for CCA clinical practice.

scholarly journals Circular RNA CDR1as Exerts Oncogenic Properties Partially through Regulating MicroRNA 641 in Cholangiocarcinoma

2020 ◽  
Vol 40 (15) ◽  
Author(s):  
Dingyang Li ◽  
Zhe Tang ◽  
Zhiqiang Gao ◽  
Pengcheng Shen ◽  
Zhaochen Liu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Target Genes ◽  
Circular Rna ◽  
Tumor Xenograft ◽  
Mrna Levels ◽  
Cell Behaviors ◽  
Xenograft Growth ◽  
Tumor Xenograft Growth

ABSTRACT It has been found that the circular RNA (circRNA) CDR1as is upregulated in cholangiocarcinoma (CCA) tissues. In this study, we tried to explore the roles of CDR1as in CCA. CDR1as was overexpressed or knocked down in human CCA cells to assess the effects of CDR1as on cell behaviors and tumor xenograft growth. In vitro, the CDR1as level was significantly increased in CCA cell lines. The results showed that CDR1as promoted the cell proliferation, migration, invasion, and activation of the AKT3/mTOR pathway in CCA cells. Moreover, miR-641, a predicted target microRNA (miRNA) of CDR1as, could partially reverse the effects of CDR1as on cell behaviors in CCA cells. Furthermore, CDR1as improved tumor xenograft growth, and it could be attenuated by miR-641 in vivo. Additionally, CDR1as expression was inversely correlated with miR-641 in CCA cells, and miR-641 could directly bind with CDR1as and its target genes, the AKT3 and mTOR genes. Mechanistically, CDR1as could bind with miR-641 and accelerate miR-641 degradation, which possibly leads to the upregulation of the relative mRNA levels of AKT3 and mTOR in RBE cells. In conclusion, our findings indicated that CDR1as might exert oncogenic properties, at least partially, by regulating miR-641 in CCA. CDR1as and miR-641 could be considered therapeutic targets for CCA.

T1374 EZH2 Knock Down by Lentiviral shRNA Inhibits Pancreatic Ductal Adeno-Carcinoma Cell Proliferation In Vitro and Tumor Growth In Vivo

2010 ◽  
Vol 138 (5) ◽  
pp. S-548
Author(s):  
Ramesh B. Batchu ◽  
Aamer Qazi ◽  
Shelly Seward ◽  
Masood A. Shammas ◽  
Sreedhar Chamala ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Carcinoma Cell ◽  
Knock Down ◽  
Adeno Carcinoma ◽  
Proliferation In Vitro

Dehydroepiandrosterone-sulfate inhibits pancreatic carcinoma cell proliferation in vitro and in vivo

Surgery ◽  
1997 ◽  
Vol 121 (4) ◽  
pp. 392-397 ◽  
Author(s):  
W.Scott Melvin ◽  
Laszlo G Boros ◽  
Peter Muscarella ◽  
James L Brandes ◽  
Jerome A Johnson ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Pancreatic Carcinoma ◽  
Carcinoma Cell ◽  
Dehydroepiandrosterone Sulfate ◽  
Proliferation In Vitro

miR-92b-3p Promotes Colorectal Carcinoma Cell Proliferation, Invasion, and Migration by Inhibiting FBXW7 In Vitro and In Vivo

2018 ◽  
Vol 37 (5) ◽  
pp. 501-511 ◽  
Author(s):  
Lei Gong ◽  
Mingyang Ren ◽  
Zhenbing Lv ◽  
Yuling Yang ◽  
Ziwei Wang
Keyword(s):  
Cell Proliferation ◽  
Colorectal Carcinoma ◽  
Carcinoma Cell ◽  
Invasion And Migration ◽  
And Migration

Inhibition of mammary carcinoma cell proliferation in vitro and tumor growth in vivo by bee venom

Toxicon ◽  
2003 ◽  
Vol 41 (7) ◽  
pp. 861-870 ◽  
Author(s):  
Nada Oršolić ◽  
Lidija Šver ◽  
Srđan Verstovšek ◽  
Svjetlana Terzić ◽  
Ivan Bašić
Keyword(s):  
Cell Proliferation ◽  
Tumor Growth ◽  
Mammary Carcinoma ◽  
Carcinoma Cell ◽  
Bee Venom ◽  
Mammary Carcinoma Cell ◽  
Proliferation In Vitro
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