scholarly journals Expression of Substance P, Calcitonin Gene-Related Peptide and Vascular Endothelial Growth Factor in Human Dental Pulp Under Different Clinical Stimuli

2020 ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Luis Fernando Lopez-Moncayo ◽  
Hernan Dario Munoz-Alvear ◽  
Jose Francisco Gomez-Sosa ◽  
Luis Eduardo Diaz-Barrera ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Control Group ◽  
Vascular Endothelial ◽  
Calcitonin Gene ◽  
Registration Number ◽  
Human Dental Pulp ◽  
Orthodontic Forces ◽  
Post Hoc ◽  
Endothelial Growth Factor ◽  
Occlusal Interferences

Abstract Background To quantify the expression of SP, CGRP, and VEGF in human dental pulps as a response to occlusal interferences, moderate orthodontic forces, and occlusal interferences plus moderate orthodontic forces simultaneously.Methods Forty human dental pulp samples were collected from healthy premolars indicated for extraction for orthodontic reasons. The teeth were divided into four groups with 10 samples each: Healthy pulp control group, occlusal interference group, moderate orthodontic forces group; and occlusal interferences plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The ANOVA test was performed to establish significant differences between groups and Tukey’s HSD post hoc comparisons were also performed.Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal interferences and the control groups, with statistically significant differences between all groups (ANOVA p<0.001) and post-hoc comparisons (Tukey HSD p<0.001).Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal interferences combined with moderate orthodontic forces, as compared with when these two stimuli are applied independently. Peptides expression is directly proportional to the magnitude of the stimulus.Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive ResponseTrial registration number: NCT03804034Date of registration: 01/15/2019 Retrospectively registeredURL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1

scholarly journals Expression of Substance P, Calcitonin Gene-Related Peptide and Vascular Endothelial Growth Factor in human dental pulp under different clinical stimuli

2020 ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Luis Fernando Lopez-Moncayo ◽  
Hernan Dario Munoz-Alvear ◽  
Jose Francisco Gomez-Sosa ◽  
Luis Eduardo Diaz-Barrera ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Control Group ◽  
Vascular Endothelial ◽  
Calcitonin Gene ◽  
Registration Number ◽  
Human Dental Pulp ◽  
Orthodontic Forces ◽  
Post Hoc ◽  
Endothelial Growth Factor ◽  
Trauma Group

Abstract Background To quantify the expression of SP, CGRP, and VEGF in human dental pulps as a response to occlusal trauma, moderate orthodontic forces, and occlusal trauma plus moderate orthodontic forces simultaneously.Methods Forty human dental pulp samples were collected from healthy premolars indicated for extraction for orthodontic reasons in 20 patients. Patients were divided into four groups with 10 samples each (5 lower and 5 upper premolars from each patient): Healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The ANOVA test was performed to establish significant differences between groups and Tukey’s HSD post hoc comparisons were also performed.Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statistically significant differences between all groups (ANOVA p<0.001). All pairwise post-hoc comparisons were also significant (Tukey HSD p<0.001).Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently. Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive ResponseTrial registration number: NCT03804034Date of registration: 01/15/2019 Retrospectively registeredURL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1

scholarly journals Expression of substance P, calcitonin gene-related peptide and vascular endothelial growth factor in human dental pulp under different clinical stimuli

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Javier Caviedes-Bucheli ◽  
Luis Fernando Lopez-Moncayo ◽  
Hernan Dario Muñoz-Alvear ◽  
Jose Francisco Gomez-Sosa ◽  
Luis Eduardo Diaz-Barrera ◽  
...  
Keyword(s):  
Dental Pulp ◽  
Defense Mechanism ◽  
Initial Step ◽  
Control Group ◽  
List Type ◽  
Registration Number ◽  
Human Dental Pulp ◽  
Orthodontic Forces ◽  
Post Hoc ◽  
Endothelial Growth Factor

Abstract Background The aim of this study was to measure the dental pulp inflammatory response through neuropeptides (SP and CGRP) as a response to occlusal trauma, orthodontic movements and a combination of both, as well as the angiogenic defense mechanism through VEGF expression, which could be the initial step to mineralized tissue formation. Methods Forty human dental pulp samples were collected from healthy first premolars with extraction indicated due to orthodontic reasons from a sample of 20 patients. Patients were divided into four groups with 10 premolars each (1 mandibular and 1 maxillary premolar from each patient): healthy pulp control group, occlusal trauma group, moderate orthodontic forces group; and occlusal trauma plus moderate orthodontic forces group. Stimuli were applied for 24 h before tooth extraction in all experimental groups. All samples were processed, and SP, CGRP, and VEGF were measured by radioimmunoassay. The Kruskal–Wallis test was performed to assess significant differences among groups and Mann–Whitney’s U post hoc pairwise comparisons were also performed. Results The highest increase in SP, CGRP, and VEGF expressions was found in the occlusal trauma plus orthodontic forces group, followed by the moderate orthodontic forces, the occlusal trauma and the control groups, with statistically significant differences between all groups for each of the 3 peptides analyzed (Kruskal–Wallis p < 0.001). All possible pairwise post-hoc comparisons were also significant for each peptide analyzed (Mann–Whitney’s U p < 0.001). Conclusion SP, CGRP, and VEGF expressions significantly increase in human dental pulps when stimulated by occlusal trauma combined with moderate orthodontic forces, as compared with these two stimuli applied independently. Name of the registry: Importance of Neurogenic Inflammation in the Angiogenic Response of the Dental Pulp as a Defensive Response. Trial registration number: NCT03804034. Date of registration: 01/15/2019 Retrospectively registered. URL of trial registry record: https://clinicaltrials.gov/ct2/show/NCT03804034?term=NCT03804034&draw=2&rank=1.

Analysis of endothelial precursor cells in chronic migraine: A case-control study

Cephalalgia ◽  
2012 ◽  
Vol 33 (4) ◽  
pp. 236-244 ◽  
Author(s):  
Agustin Oterino ◽  
Maria Toriello ◽  
Enrique Palacio ◽  
Vicente G Quintanilla ◽  
Nuria Ruiz-Lavilla ◽  
...  
Keyword(s):  
Surrogate Marker ◽  
Vascular Risk Factor ◽  
Endothelial Activation ◽  
Endothelial Damage ◽  
Control Group ◽  
Vascular Endothelial ◽  
Circulating Cells ◽  
Colony Forming Units ◽  
Calcitonin Gene ◽  
Endothelial Growth Factor

Background: Migraine has been considered a vascular risk factor especially in young women. Factors predisposing to endothelial damage in migraine are still being debated. The insufficiency of circulating endothelial precursor circulating cells (EPCs) suggested a link between migraine and cardiovascular risk. This research aimed to study a subtype of EPCs, those expressing e-selectin, to assess endothelial activation and, therefore, endothelial dysfunction in migraine. Methods: Consecutive headache patients ( n = 99) and 35 adjusted controls were recruited. Total EPCs, defined as CD34+/KDR+ cells, and EPC colony-forming units (CFUs) were assayed. We identified as “early” EPCs those CD62E– EPCs, and “late” EPCs, CD62E+, a surrogate marker for endothelial damage. Plasmatic calcitonin-gene related protein (CGRP) and vascular-endothelial growth factor (VEGF) were analyzed. Results: We did not find differences in the total number of CFUs among clinical groups. Means of total CD34+/KDR+ and “early” EPCs were not significant among clinical groups. Nevertheless, the mean of “late” EPCs was lower (log10-transformed mean = 1.715; SD = 0.393) in the control group than in the migraine patients (log10-transformed mean = 2.167; SD = 0.685), even after adjustment by VEGF plasma level and other confounding factors. Linear regression analyses disclosed significant predictors for “late” EPCs for controls vs migraine (β = 0.452 SE ± 0.13; p = 0.001). We did not observe differences between migraine with or without aura. Conclusion: We observed higher number of activated EPCs in migraine patients than in controls. CD62E+ EPCs might be considered a marker for vascular damage in migraine patients.

scholarly journals Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation:In VivoStudy

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Daniel Chavarria-Bolaños ◽  
Hector Flores-Reyes ◽  
Nelson Lombana-Sanchez ◽  
Bernardino Cerda-Cristerna ◽  
Amaury Pozos-Guillen
Keyword(s):  
Dental Pulp ◽  
Endogenous Opioids ◽  
Control Group ◽  
Pulp Tissue ◽  
Calcitonin Gene ◽  
Dental Pulp Tissue ◽  
Human Dental Pulp ◽  
Increasing Trends ◽  
Inflammation Group ◽  
Different Levels

Purpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP),β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion.Methods. Eight patients were selected according to preestablished inclusion criteria. From each patient, two premolars (indicated for extraction due to orthodontic reasons) were randomly assigned to two different groups: the asymptomatic inflammation group (EXPg), which would undergo controlled intrusive force for seven days, and the control group (CTRg), which was used to determine the basal levels of each substance. Once extracted, dental pulp tissue was prepared to determine the expression levels of both neuropeptides and endogenous opioids by radioimmunoassay (RIA).Results. All samples from the CTRg exhibited basal levels of both neuropeptides and endogenous opioids. By day seven, all patients were asymptomatic, even when all orthodontic-intrusive devices were still active. In the EXPg, the SP and CGRP exhibited statistically significant different levels. Although none of the endogenous opioids showed statistically significant differences, they all expressed increasing trends in the EXPg.Conclusions. SP and CGRP were identified in dental pulp after seven days of controlled orthodontic intrusion movement, even in the absence of pain.

scholarly journals Increased VEGF-A Expression of Human Dental Pulp Stem Cells (hDPSCs) Cultured with Advanced Platelet Rich Fibrin (A-PRF)

2021 ◽  
Vol 15 (1) ◽  
pp. 569-574
Author(s):  
Dini Asrianti Bagio ◽  
Indah Julianto ◽  
Anggraini Margono ◽  
Endang Suprastiwi
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Control Group ◽  
Platelet Rich Fibrin ◽  
Significant Difference ◽  
Angiogenesis Process ◽  
Human Dental Pulp ◽  
Post Hoc

Background: VEGF-A expression of human dental pulp stem cells (hDPSCs) can induce the angiogenesis process of dental pulp regeneration. This in vitro study aimed to analyze the effect of various concentrations of Advanced Platelet Rich Fibrin (A-PRF) conditioned media (CM) on the increased expression of vascular endothelial growth factor-A (VEGF-A) of hDPSCs. Methods: hDPSCs were collected from ten third molars extracted from nine healthy donors, cultured, and then harvested at the end of the third passage. The hDPSCs were seeded in four different CM (control group: hDPSCs + DMEM; 1% A-PRF CM group: hDPSCs + 1% A-PRF CM; 5% A-PRF CM group: hDPSCs + 5% A-PRF CM; 10% A-PRF CM group: hDPSCs + 10% A-PRF CM). All of the groups were cultured in biological triplicates (Triplo) and observed for 5, 12, and 24 hours. The VEGF-A protein expression of hDPSCs was measured using human VEGF-A ELISA at a wavelength of 405 nm. Data was analyzed with Kruskal Wallis and post hoc Mann Whitney test with p<0.05. Results: The VEGF-A expression rate of hDPSCs among all groups was statistically significantly different at 5, 12 and 24 hours of observations (p<0.05). Post hoc analysis test showed a statistically significant difference of hDPSCs’s VEGF-A expression between 5% A-PRF groups compared to other groups at 5 and 12 hours of observation (p<0.05). However, there were no statistically significant differences observed of hDPSCs’ VEGF-A expression at 24 hours of observation between 1%, 5% and 10% A-PRF groups (p>0.05). Conclusion: 5% A-PRF CM was superior in increasing VEGF-A expression of hDPSCs at 5, 12 and 24 hours of observations.

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