Design And Preparation of New Decellularized Extracellular Matrix/Alginate Composite Membranes For Biomimetic Bioinks Via Supercritical Carbon Dioxide Fluid Treatments

Mapping Intimacies ◽

10.21203/rs.3.rs-622568/v1 ◽

2021 ◽

Author(s):

Ching-Cheng Huang

Keyword(s):

Carbon Dioxide ◽

Extracellular Matrix ◽

Supercritical Carbon Dioxide ◽

Thermo Gravimetric Analysis ◽

Composite Membranes ◽

Gravimetric Analysis ◽

Time Saving ◽

Decellularized Extracellular Matrix ◽

Cellular Components ◽

Supercritical Carbon

Abstract Tissue-engineering was important and popular which combine medical applications and engineering materials knowledge, just like decellularization techniques were employed to remove the cellular components from porcine elastic cartilages, leaving a native decellularized extracellular matrix(dECM) composition and architecture integrity of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans. Particularly, an extraction process of supercritical carbon dioxide(ScCO2) was used to remove cellular components from porcine skins. The porcine skins must remove lipids and other impurities by using ScCO2 procedure. In this study, a series of new composite membranes with decellularized scaffolds could be designed and obtained from porcine skin tissue by using supercritical carbon dioxide fluid technology. The retain decellularized extracellular matrix (dECM) and integrity scaffold-structure could be observed in the new composite membranes. This work provides a simple and time-saving method process for preparation of biomedical composite membranes with dECM scaffolds for biomimetic bioinks, which were further characterized by Fourier transform infrared spectroscopy (FTIR), thermo-gravimetric analysis (TGA), and scanning electron microscope(SEM).

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New Designed Decellularized Scaffolds for Scaffold-based Gene Therapy from Elastic Cartilages via Supercritical Carbon Dioxide Fluid and Alkaline/Protease Treatments

Current Gene Therapy ◽

10.2174/1566523219666210618151843 ◽

2021 ◽

Vol 21 ◽

Author(s):

Ching-Cheng Huang

Keyword(s):

Carbon Dioxide ◽

Tissue Engineering ◽

Gene Therapy ◽

Supercritical Carbon Dioxide ◽

Potential Application ◽

Alkaline Protease ◽

Thermo Gravimetric Analysis ◽

Gravimetric Analysis ◽

Cellular Components ◽

Supercritical Carbon

Background: Scaffold-based gene therapy provides a promising approach for tissue engineering, which is important and popular, that combines medical applications with engineering materials knowledge. Objective: The decellularization techniques were employed to remove the cellular components from porcine elastic cartilages, leaving a native decellularized extracellular matrix(dECM) composition and architecture integrity of largely insoluble collagen, elastin, and tightly bound glycosaminoglycans. For newly designed collagen scaffold samples, elastic cartilages was hydrolyzed by protease with different concentrations. In this way, it could gain state completely and clearly. Methods: An extraction process of supercritical carbon dioxide(ScCO2) was used to remove cellular components from porcine elastic cartilage. The dECM scaffolds with collagen must be characterized by Fourier transform infrared spectroscopy(FTIR), thermo-gravimetric analysis (TGA), and scanning electron microscope(SEM). Results: The study provided a new treatment combined with supercritical carbon dioxide and alkaline/protease to prepare dECM scaffolds with hole-scaffold microstructures and introduce into a potential application on osteochondral tissue engineering using scaffold-based gene therapy. The new process is simple and efficient. The pore-scaffold microstructures were observed in dECM scaffolds derived from porcine elastic cartilages. The Tdmax values of the resulting dECM scaffolds were observed over 330oC. Conclusion: A series of new scaffolds were successfully obtained from porcine tissue by using ScCO2 and alkaline/enzyme treatments such as an aqueous mixing solution of NH4OH and papain. The dECM scaffolds with high thermal stability were obtained. The resulting scaffold with clean pore-scaffold microstructure could be a potential application for scaffold-based gene therapy.

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Characteristics and Preparation of Designed Alginate-Based Composite Scaffold Membranes with Decellularized Fibrous Micro-Scaffold Structures from Porcine Skin

Polymers ◽

10.3390/polym13203464 ◽

2021 ◽

Vol 13 (20) ◽

pp. 3464

Author(s):

Ching-Cheng Huang

Keyword(s):

Composite Scaffold ◽

Thermo Gravimetric Analysis ◽

Biological Properties ◽

Extracellular Matrices ◽

Gravimetric Analysis ◽

Porcine Skin ◽

Thermo Gravimetric ◽

Time Saving ◽

Decellularized Extracellular Matrix ◽

Scaffold Structure

Alginate-based composite scaffold membranes with various ratios of decellularized extracellular matrices could be designed and obtained from porcine skin tissue by using supercritical carbon dioxide fluid technology. Retention of decellularized extracellular matrix (dECM) and scaffold-structure integrity was observed. This work provides a simple and time-saving process for the preparation of biomedical alginate-based composite scaffold membranes with fibrous dECM micro-scaffolds, which were further characterized by Fourier transform infrared spectroscopy (FTIR), thermo-gravimetric analysis (TGA), and scanning electron microscope (SEM). The introduction of fibrous dECM micro-scaffolds enhanced the thermal stability and provided expected effects on the biological properties of the designed composite scaffold membranes in regenerative applications.

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