Identification of Potential Crucial Immune-related Genes and Key Pathways in Oral Lichen Planus

Abstract Oral lichen planus (OLP) is a chronic autoimmune oral mucosal disease which seriously affect the life quality of the patients. But till now, the exact etiology and pathogenesis of OLP still remains unclear. The aim of our study was to find the key molecules and pathway involved in the pathogenesis mechanisms of OLP, therefore, provide reference for identifying more effective therapeutic strategies for OLP. Data from GSE52130 were downloaded from GEO data sets for analysis, and a total of 255 differentially expressed genes were selected out. Then by means of a series of gene enrichment analysis, we illustrated that most of these important genes were enriched in the focal adhesion pathway and metabolism pathways. In addition, we also constructed a protein-protein interaction (PPI) network and identified 8 hub genes which may play the important role in the development of OLP, including THBS2, COL3A1, IGFBP3, COL1A2, COL1A1, PTGS2, IL1B, ARG1. Lastly, we also predicted transcription factors of these hub genes, then constructed the corresponding transcription regulatory network. Taken together, our data indicated that the focal adhesion pathway and metabolism pathways, as well as 8 crucial immune-related genes might be most likely involved in the development of OLP, which would provide important information and objective basis for elucidating the pathogenesis of OLP and providing more effective targeted immunotherapy strategies for OLP.

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RNA-Seq based transcriptome analysis in oral lichen planus

Hereditas ◽

10.1186/s41065-021-00202-z ◽

2021 ◽

Vol 158 (1) ◽

Author(s):

Haoyu Wang ◽

Yiwen Deng ◽

Siqi Peng ◽

Li Yan ◽

Hui Xu ◽

...

Keyword(s):

T Cell ◽

Wnt Signaling ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Wnt Signaling Pathway ◽

Enrichment Analysis ◽

Rna Seq ◽

Hub Genes ◽

Mucosal Tissues ◽

Oral Lichen

Abstract Objectives Oral lichen planus (OLP) is a T cell-mediated autoimmune disease recognized as an oral potential malignant disorder (OPMD) with the precise mechanism unknown. This study focused on the transcriptional profiles of OLP to elucidate its potential pathogenesis. Methods We conducted RNA sequencing on matched 6 OLP tissues and 6 normal oral mucosal tissues. Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and weighted gene co-expression network analysis (WGCNA) were performed on differentially expressed genes (DEGs). We utilized qRT-PCR to validated the top dysregulated genes and hub genes in another 10 pairs of specimens. Results A total of 153 DEGs (p-values< 0.05) were detected from RNA-Seq. According to GO and KEGG analysis, the dysregulated genes were mainly related to T cell related pathway and Wnt signaling. Based on the WGCNA analysis, 5 modules with high intramodular connectivity and hub genes in each module were gained. Conclusions RNA-Seq and bioinformatic methods offered a valuable understanding of the biological pathways and key genes in the regulation of OLP. The identified DEGs and hub genes categorized into 2 groups including T cell regulation and inflammation and Wnt signaling pathway may serve as potential novel molecular targets for therapy.

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RNA-Seq based transcriptome analysis in oral lichen planus

10.21203/rs.3.rs-351759/v1 ◽

2021 ◽

Author(s):

Haoyu Wang ◽

Yiwen Deng ◽

Siqi Peng ◽

Li Yan ◽

Hui Xu ◽

...

Keyword(s):

T Cell ◽

Wnt Signaling ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Wnt Signaling Pathway ◽

Enrichment Analysis ◽

Rna Seq ◽

Hub Genes ◽

Mucosal Tissues ◽

Oral Lichen

Abstract Objectives: Oral lichen planus (OLP) is a T cell-mediated autoimmune disease recognized as an oral potential malignant disorder (OPMD) with the precise mechanism unknown. This study focused on the transcriptional profiles of OLP to elucidate its potential pathogenesis. Methods: We conducted RNA sequencing on matched 6 OLP tissues (n = 6) and 6 normal oral mucosal tissues (n = 6). Gene Ontology (GO) enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway and weighted gene co-expression network analysis (WGCNA) were performed on differentially expressed genes (DEGs). We utilized qRT-PCR to validated the top dysregulated genes and hub genes in another 10 pairs of specimens.Results: A total of 153 DEGs (p-values< 0.05) were detected from RNA-Seq. According to GO and KEGG analysis, the dysregulated genes were mainly related to T cell related pathway and Wnt signaling. Based on the WGCNA analysis, 5 modules with high intramodular connectivity and hub genes in each module were gained.Conclusions: RNA-Seq and bioinformatic methods offered a valuable understanding of the biological pathways and key genes in the regulation of OLP. The identified DEGs and hub genes categorized into 2 groups including T cell regulation and inflammation and Wnt signaling pathway may serve as potential novel molecular targets for therapy.

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Bioinformatics analysis of differentially expressed genes and their potentially interacting miRNAs in oral lichen planus

10.21203/rs.3.rs-952216/v2 ◽

2021 ◽

Author(s):

Churen Zhang ◽

Ruoran Sun

Keyword(s):

Lichen Planus ◽

Differentially Expressed Genes ◽

Oral Mucosa ◽

Oral Lichen Planus ◽

Kegg Pathway ◽

Functional Enrichment ◽

Differentially Expressed ◽

Ppi Network ◽

Hub Genes ◽

Oral Lichen

Abstract Background. Among the diseases of oral mucosa, oral lichen planus (OLP) is characterized by chronic autoimmune/autoinflammation. However, the etiology and pathogenesis of OLP were still limited. The aim of this research was to identify the differentially expressed genes and their potentially interacted miRNAs in OLP to provide a possible explanation of the pathogenesis of OLP and therapeutic biomarkers.Methods. The OLP microarray dataset (GSE52130) was download from the Gene Expression Omnibus (GEO) database. R software was used to identify differentially expressed genes between the OLP samples and normal oral mucosa. Functional enrichment analysis of DEGs, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, were conducted. Protein–protein interaction (PPI) network analysis was performed in the STRING database. CytoHubba in the Cytoscape software was applied to determining the top 10 hub genes, whose relative miRNA was identified through RNA Interactome Database.Results. Overall, 627 DEGs was identified in OLP samples, including 351 highly expressed genes and 276 lowly expressed genes. GO analysis indicated that the epidermal differentiation was mostly enriched. For the KEGG pathway, the DEGs in OLP samples were mostly involved in Staphylococcus aureus infection, Estrogen signaling pathway, Serotonergic synapse and Histidine metabolism. Top 10 hub genes including LOR, LCE3D, LCE3E, LCE1B, LCE2B, SPRR2E, SPRR2G, LCE2A, RPTN and CDSN were identified from the PPI network. The miRNA (hsa-miR-98-5p) was regarded as the mostly possible miRNA involved in OLP.

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Gyvenimo kokybė sergant erozineopine ir retikuline plokščiosios kerpligės formomis

Sveikatos mokslai ◽

10.5200/sm-hs.2013.018 ◽

2013 ◽

Vol 23 (1) ◽

pp. 100-102

Author(s):

Arūnas Rimkevičius ◽

Jolanta Aleksejūnienė ◽

Alina Pūrienė ◽

Mindaugas Gaigalas

Keyword(s):

Lichen Planus ◽

Oral Lichen Planus ◽

Life Quality ◽

University Hospital ◽

Quality Aspects ◽

Vilnius University ◽

Physical Comfort ◽

Oral Lichen ◽

Reticular Form

The present work analyses various life quality aspects of 79 patients with oral lichen planus, who were examined in the Žalgiris Clinic of Vilnius University Hospital in the period 2009-2012. Oral lichen planus had different forms of the disease and individual experience, which impacted quality of life. The erosive-ulcerative form of the disease as compared to reticular form had more detrimental influence on psychological, social or physical comfort of patients. A high percentage of patients (48,3% erosive-ulcerative form and 44,5% reticular form) were concerned about their future with oral lichen planus.

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Bioinformatics analysis of differentially expressed genes and their potentially interacting miRNAs in oral lichen planus

10.21203/rs.3.rs-952216/v1 ◽

2021 ◽

Author(s):

Churen Zhang ◽

Ruoran Sun

Keyword(s):

Lichen Planus ◽

Differentially Expressed Genes ◽

Oral Lichen Planus ◽

Kegg Pathway ◽

Microarray Dataset ◽

Epidermal Differentiation ◽

Differentially Expressed ◽

Ppi Network ◽

Hub Genes ◽

Oral Lichen

Abstract Background Oral lichen planus (OLP) was a common oral mucosal disease. However, the etiology and pathogenesis of OLP were still limited. This research was designed to identify the differentially expressed genes and relative miRNAs in OLP. Methods and Results The OLP microarray dataset (GSE52130) was download from the Gene Expression Omnibus (GEO) database. R software was used to identify differentially expressed genes between the OLP samples and normal oral mucosa. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway conducted. Protein–protein interaction (PPI) network analysis was performed in the STRING database. CytoHubba in the Cytoscape software was applied to determining the top 10 hub genes, whose relative miRNA was identified through RNA Interactome Database. Overall, 627 DEGs was identified in OLP samples, including 351 highly expressed genes and 276 lowly expressed genes. GO analysis indicated that the epidermal differentiation was mostly enriched. For the KEGG pathway, the DEGs in OLP samples were mostly involved in Staphylococcus aureus infection. Top 10 hub genes were identified from the PPI network. The miRNA (hsa-miR-98-5p) was regarded as the mostly possible miRNA involved in OLP. Conclusions The epidermal differentiation complex and functional miRNAs (hsa-miR-98-5p, hsa-let-7e-5p, hsa-let-7f-5p) were potential biomarkers of OLP

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