scholarly journals Genome Characterization of the Novel Lytic Genome Sequence of Phage YUEEL01 of the Myoviridae Family

2021 ◽  
Author(s):  
Gurusamy Raman ◽  
Ayyaru Sivasankaran ◽  
SeonJoo Park ◽  
Young-Ho Ahn
Keyword(s):  
Wastewater Treatment ◽  
Municipal Wastewater ◽  
Animal Health ◽  
Gc Content ◽  
Phenotypic Characterization ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Urban Hospital ◽  
Protein Coding ◽  
Antibiotic Resistant

Abstract Antimicrobial resistance is a global concern due to its rapid emergence in the environment and the associated high risk to human and animal health. Municipal wastewater, including urban, hospital, and pharmaceutical effluent, is the main source of antibiotic and antibiotic resistant bacteria (ARB) contamination, and biological processes are commonly used for wastewater treatment. Biologically based strategies seem to be a promising approach to effective integrated ARB control since they can focus on the bases of antibiotic resistance. To develop an effective bacteriophage against multi-drug resistance (MDR) microbes in municipal wastewater, phage YUEEL01 was isolated from a livestock farm, and its genome sequenced. Phenotypic characterization suggested that the phage is a member of the Myoviridae family, and that it encodes 168,266 bp with an overall GC content of 35.4% had 259 putative protein-coding genes, 11 tRNA and six hypothetical genes. A number of putative DNA replication and regulation, DNA packing and structure and host lysis genes were identified. Further, whole genome phylogenetic analysis demonstrated that phage YUEEL01 is closely related to phage slur03. Elucidation of the YUEEL01 phage might be helpful when developing antibacterial tools for controlling MDR bacteria in wastewater treatment systems.

scholarly journals Removal of antibiotic resistant E. coli in two Norwegian wastewater treatment plants and by nano- and ultra-filtration processes

2017 ◽  
Vol 77 (4) ◽  
pp. 1115-1126 ◽  
Author(s):  
Carsten Ulrich Schwermer ◽  
Pawel Krzeminski ◽  
Aina Charlotte Wennberg ◽  
Christian Vogelsang ◽  
Wolfgang Uhl
Keyword(s):  
Wastewater Treatment ◽  
Membrane Filtration ◽  
Municipal Wastewater ◽  
Wastewater Treatment Plants ◽  
Considerable Change ◽  
Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli ◽  
Physical Chemical ◽  
Ultra Filtration

Abstract The effectivity of different treatment stages at two large wastewater treatment plants (WWTPs) located in Oslo, Norway, to remove antibiotic resistant Escherichia coli from municipal wastewater was investigated. The WWTPs were effective in reducing the total cultivable E. coli. The E. coli in WWTP samples were mainly resistant to ampicillin (6–27%) and trimethoprim-sulfamethoxazole (5–24%), and, to a lesser extent, tetracycline (3–14%) and ciprofloxacin (0–7%). In the first WWTP, a clear decrease in the percentage of E. coli resistant to these antibiotics was found, with the main removal occurring during physical/chemical treatment. In the second WWTP, the percentage of cultivable resistant E. coli did not display a considerable change. During laboratory-scale membrane filtration of WWTP effluents using ultrafiltration (UF) and nanofiltration (NF) membranes, all E. coli, including those resistant to antibiotics, were removed completely. The results imply that UF and NF processes are potent measures to remove antibiotic resistant bacteria (ARB) during post-treatment of WWTP effluents, thus reducing the potential spread of antibiotic resistance in the receiving aquatic environment.

scholarly journals The occurrence of antibiotic-resistant bacteria, including Escherichia coli, in municipal wastewater and river water

2019 ◽  
Vol 100 ◽  
pp. 00061 ◽  
Author(s):  
Adriana Osińska ◽  
Ewa Korzeniewska ◽  
Monika Harnisz ◽  
Sebastian Niestępski ◽  
Piotr Jachimowicz
Keyword(s):  
Escherichia Coli ◽  
Wastewater Treatment ◽  
River Water ◽  
Municipal Wastewater ◽  
High Efficiency ◽  
Treated Wastewater ◽  
Resistant Bacteria ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
E Coli

Wastewater treatment plants (WWTPs) are major reservoirs of antibiotic-resistant bacteria (ARB) which are transported to the natural environment with discharged effluents. Samples of untreated wastewater (UWW) and treated wastewater (TWW) from four municipal WWTPs and samples of river water collected upstream (URW) and downstream (DRW) from the effluent discharge point were analyzed in the study. The total counts of bacteria resistant to β-lactams and tetracyclines and the counts of antibiotic-resistant Escherichia coli were determined. Antibiotic-resistant bacteria, including antibiotic-resistant E. coli, were removed with up to 99.9% efficiency in the evaluated WWTPs. Despite the above, ARB counts in TWW samples were high at up to 1.25x105 CFU/mL in winter and 1.25x103 CFU/mL in summer. Antibiotic-resistant bacteria were also abundant (up to 103 CFU/ml) in URW and DRW samples collected in winter and summer. In both UWW and TWW samples, the counts of ARB and antibiotic-resistant E. coli were at least one order of magnitude lower in summer than in winter. The study revealed that despite the high efficiency of bacterial removal in the wastewater treatment processes, considerable amounts of ARB are released into the environment with TWW and that the percentage of ARB in total bacterial counts increases after wastewater treatment.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

scholarly journals Antibiotic resistant and extended-spectrum β-lactamase producing faecal coliforms in wastewater treatment plant effluent

2019 ◽  
Author(s):  
Cian Smyth ◽  
Aidan O’Flaherty ◽  
Fiona Walsh ◽  
Thi Thuy Do
Keyword(s):  
Antibiotic Resistance ◽  
Wastewater Treatment ◽  
Control Point ◽  
Treated Wastewater ◽  
Multi Drug Resistance ◽  
Resistant Bacteria ◽  
Faecal Coliforms ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Extended Spectrum

AbstractWastewater treatment plants (WWTPs) provide optimal conditions for the maintenance and spread of antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARGs). In this work we describe the occurrence of antibiotic resistant faecal coliforms and their mechanisms of antibiotic resistance in the effluent of two urban WWTPs in Ireland. Effluent samples were collected from two WWTPs in Spring and Autumn of 2015 and 2016. The bacterial susceptibility patterns to 13 antibiotics were determined. The phenotypic tests were carried out to identify AmpC or extended-spectrum β-lactamase (ESBL) producers. The presence of ESBL genes were detected by PCR. Plasmids carrying ESBL genes were transformed into Escherichia coli DH5α recipient and underwent plasmid replicon typing to identify incompatibility groups. More than 90% of isolated faecal coliforms were resistant to amoxicillin and ampicillin, followed by tetracycline (up to 39.82%), ciprofloxacin (up to 31.42%) and trimethoprim (up to 37.61%). Faecal coliforms resistant to colistin and imipenem were detected in all effluent samples. Up to 53.98% of isolated faecal coliforms expressed a multi-drug resistance (MRD) phenotype. AmpC production was confirmed in 5.22% of isolates. The ESBL genes were confirmed for 11.84% of isolates (9.2% of isolates carried blaTEM, 1.4% blaSHV-12, 0.2% blaCTX-M-1 and 1% blaCTX-M-15). Plasmids extracted from 52 ESBL isolates were successfully transformed into recipient E. coli. The detected plasmid incompatibility groups included the IncF group, IncI1, IncHI1/2 and IncA/C. These results provide evidence that treated wastewater is polluted with ARB and MDR faecal coliforms and are sources of ESBL-producing, carbapenem and colistin resistant Enterobacteriaceae.ImportanceAntibiotic resistant bacteria (ARB) are an emerging environmental concern with a potential impact on human health. The results provide the evidence that treated wastewater is polluted with antibiotic resistant bacteria containing mobile resistance mechanisms of importance to clinical treatment of pathogens and multi-drug resistant (MDR) faecal coliforms. They are sources of relatively high proportions of ESBL-producing Enterobacteriaceae, and include carbapenem and colistin resistant Enterobacteriaceae. The significance of this study is the identification of the role of WWTPs as a potential control point to reduce or stop the movement of ESBL, MDR and colistin resistant bacteria into the environment from further upstream sources, such as human or animal waste.

Sign in / Sign up

Export Citation Format

Share Document