scholarly journals Inline monitoring of high cell density cultivation of Scenedesmus rubescens in a mesh ultra-thin layer photobioreactor by Photon Density Wave spectroscopy

Author(s):  
Michael Sandmann

Abstract Objective Due to multiple light scattering that occurs inside and between cells, quantitative optical spectroscopy in turbid biological suspensions is still a major challenge. This includes also optical in-line determination of biomass in bioprocessing. Photon Density Wave (PDW) spectroscopy, a technique based on multiple light scattering, enables the independent and absolute determination of optical key parameters of concentrated cell suspensions, which allow to determine biomass during cultivation. Results A unique reactor type, called “mesh ultra-thin layer PBR” was used to create a highly concentrated algal suspension. PDW spectroscopy measurements were carried out continuously in the reactor without any need of sampling or sample preparation, over 3 weeks, and with 10-minutes time resolution. Conventional dry matter content and coulter counter measurements have been employed as established off-line reference analysis. The PBR allowed peak cell dry weight (CDW) of 33.4 g L−1. It is shown that the reduced scattering coefficient determined by PDW spectroscopy is strongly correlated with the biomass concentration in suspension and is thus suitable for process understanding. The reactor in combination with the fiber-optical measurement approach will lead to a better process management.

2020 ◽  
pp. 72-75
Author(s):  
I. T. Balashova ◽  
L. V. Bespalko ◽  
A. V. Molchanova ◽  
E. V. Pinchuk ◽  
N. E. Maschenko

Relevance. Vertical farming – is a new and advanced direction in greenhouse vegetable cultivation. Expansion biodiversity of plants for vertical farming occur with help of green leafy vegetables. Plants of Lamiaceae family are well known as aromatic and medicinal plants with high content of substances with antioxidant activity. It allows use these plants as a base for the functional nutrition. Leafy parts the plants of Lamiaceae family may be used as aromatic and healthy additions to traditional foods, such as salads, soups and sauces.  Goal of the study: analysis the biochemical composition of leafy parts plants of Lamiaceaefamily, cultivated at the multi circle hydroponic construction. Materials and methods. 1) Plants: Monarda fistulosa L. (the breeding sample №5 U.P.), Monarda citriodora Cerv. ex Lag. (Simka variety), Melissa officinalisL. (Zhemchuzhina variety). 2) secondary metabolites: flavonoid glycoside linarozid and steroid glycoside moldstim. Methods. 1) cultivation of plants Lamiaceae family at the 5 circles hydroponic construction; 2) analytic methods: determination of dry matter content, determination of ascorbic acid content, determination sum of chlorophylls and carotenoids, determination sum of antioxidants; 3) statistical methods. Results. First experiment the cultivation of plants Lamiaceae family at the multi circle hydroponic construction was successful. Location of plants at different circles of hydroponic installation didn’t influence the content of dry matter, ascorbic acid and sum of chlorophylls in leafy parts of plants Monarda fistulosaL. The content of dry matter and sum of antioxidants in leafy parts of Monarda fistulosaL. plants of the first cutting is significantly higher than of other cuttings. So, we can recommend the leaves of Monarda fistulosa L. plants of the first cutting for the healthy additions in the scheme of the functional nutrition. Seed treatment with water solutions of secondary metabolites changed significantly the weight of leaves: it was increased in Monarda citriodoraCerv. ex Lag. and in Melissa officinalisL. 


2002 ◽  
Vol 46 (10) ◽  
pp. 105-114 ◽  
Author(s):  
L.H. Mikkelsen ◽  
T. Mascarenhas ◽  
P.H. Nielsen

The fraction of extractable extracellular polymeric substances (EPS) and the shear sensitivity (kSS) are key parameters with respect to sludge dewatering, affecting the dry matter content of dewatered sludge and the dewatering rate and conditioner demand, respectively. Methods are described for determination of the two key parameters by use of the same laboratory test reactor. The implications of such characterisation with respect to dewatering are discussed based on examples of application to sludge processing and novel process development for sludge minimisation.


2017 ◽  
Vol 20 (2) ◽  
Author(s):  
SIMONA GHINITA CONSTANTIN ◽  
MIRELA PRAISLER ◽  
GABRIELA IORDĂCHESCU

<p>Thyme (<em>Satureja hortensis</em>) is a popular spice for food, which is also often used as a medicine for various ailments. This paper presents an artificial intelligence method applied for the objective determination of the most important physico-chemical variables affecting the quality of thyme, i.e. Principal Component Analysis (PCA). The results show that the main properties which significantly influence the nutritional value of thyme are moisture (MOIST), dry matter content (DRYM), protein content (PROT) and, to a lesser extent, carbohydrate content (CARB). Humidity is strongly and negatively correlated with the latter three variables. The main variable that ensures the similarity between the thyme samples having the same geographical origin is the monosodium glutamate content, which generates its delicious (umami) taste.</p>


2021 ◽  
pp. 27-30
Author(s):  
Haver A. Haveri

In many livestock farms that are engaged in the breeding of cattle, both meat and dairy directions fresh pulp is mixed with molasses, and then this mass is given to the animals. Beet pulp is a by product of the beet sugar production process, representing desaccharified beet chips (80-82% by weight of processed sugar beet with a dry matter content of about 6.5-7%). The main direction of using sugar beet pulp is the use of it in the feeding of cattle for meat and milk directions. Beet pulp remains one of the most valuable feed for feeding cattle. Pulp is a highly digestible source of carbon, its main components are pectin and hemicellulose. At present, the issue of the quality of beet pulp plays a crucial role in its use for feed purposes. The volume of its implementation in feed production depends on it.


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