Genomic characteristics of a chicken infectious anemia virus strain isolated from contaminated chicken pox attenuated vaccine

Abstract Chicken infectious anemia virus (CIAV) can be transmitted by contaminated live vaccines, and causes huge economic losses. This study evaluated the contamination status of CIAV in 24 batches of vaccines by recombinase-aided amplification assay (RAA), fluorescence quantitative PCR and dot blot assay, and then determined a contaminated avian pox attenuated vaccine. The whole genome of the CIAV contaminant was then sequenced and named as JS2020-PFV (Genbank accession number: MW234428, 2296bp). It showed 94.5–99.9% identities with reference strains, and shared the closest evolution relationship with AB1K which was isolated from a chicken farm in Turkey. All these suggested that the use of CIAV contaminated live vaccine may be the reason for its epidemic in poultry.

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Newcastle disease virus-attenuated vaccine co-contaminated with fowl adenovirus and chicken infectious anemia virus results in inclusion body hepatitis-hydropericardium syndrome in poultry

Veterinary Microbiology ◽

10.1016/j.vetmic.2018.03.019 ◽

2018 ◽

Vol 218 ◽

pp. 52-59 ◽

Cited By ~ 10

Author(s):

Qi Su ◽

Yang Li ◽

Fanfeng Meng ◽

Zhizhong Cui ◽

Shuang Chang ◽

...

Keyword(s):

Newcastle Disease Virus ◽

Disease Virus ◽

Inclusion Body ◽

Newcastle Disease ◽

Attenuated Vaccine ◽

Hydropericardium Syndrome ◽

Inclusion Body Hepatitis ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus

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A New Strategy for the Detection of Chicken Infectious Anemia Virus Contamination in Attenuated Live Vaccine by Droplet Digital PCR

BioMed Research International ◽

10.1155/2019/2750472 ◽

2019 ◽

Vol 2019 ◽

pp. 1-9

Author(s):

Qiuchen Li ◽

Yubiao Zhang ◽

Fanfeng Meng ◽

Hui Jiang ◽

Guanlong Xu ◽

...

Keyword(s):

Digital Pcr ◽

Economic Losses ◽

Lymphoid Tissues ◽

Detection Technology ◽

Chicken Infectious Anemia Virus ◽

New Strategy ◽

Chicken Infectious Anemia ◽

Polymerase Chain ◽

Anemia Virus ◽

Digital Polymerase Chain Reaction

Chicken infectious anemia virus (CIAV) causes the atrophy of bone marrow hematopoietic and lymphoid tissues in chicks, leading to huge economic losses all over the world. The using of attenuated vaccine contaminated with CIAV increased the mortality and the pathogenicity of other diseases in many farms. However, it is difficult to detect the CIAV contamination by general detection technology due to the extremely low dose of CIAV in vaccines. In this study, we established a new method called droplet digital Polymerase Chain Reaction (ddPCR) to detect CIAV contamination of vaccines more sensitively and accurately. The lowest detection limitation of this method is 2.4 copies of CIAV plasmid or CIAV contamination at 0.1 EID50/1000 feathers in vaccines without any positive signals of other viruses. Besides, the sensitivity of ddPCR is 100 times greater than that of conventional PCR and 10 times greater than that of real-time PCR. The ddPCR technique is more sensitive and more intuitive. Therefore, it could be valuable for the detection of CIAV contamination in vaccines.

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Prevalence of Fowl Adenovirus Serotype 4 and Co-Infection by Immunosuppressive Viruses in Fowl with Hydropericardium Hepatitis Syndrome in Shandong Province, China

Viruses ◽

10.3390/v11060517 ◽

2019 ◽

Vol 11 (6) ◽

pp. 517 ◽

Cited By ~ 2

Author(s):

Guanliu Yu ◽

Yun Lin ◽

Yanguo Dou ◽

Yi Tang ◽

Youxiang Diao

Keyword(s):

Shandong Province ◽

Economic Losses ◽

Infection Status ◽

Adenovirus Serotype ◽

Fowl Adenovirus ◽

Chicken Infectious Anemia Virus ◽

Bursal Disease ◽

Chicken Infectious Anemia ◽

Anemia Virus ◽

Fowl Adenovirus Serotype 4

Fowl adenovirus serotype 4 (FAdV-4) is the pathogenic agent of hydropericardium hepatitis syndrome (HHS) in chickens and ducks, which has caused huge economic losses for the Chinese poultry industry since 2015. In order to objectively determine the prevalence and co-infection status of the virus in Shandong province in China, we analyzed a total of 679 clinical cases of chickens and ducks from 36 farms in the province. The results showed that the FAdV-4 infection rate was 65.2% (443/679), and the rate in breeder ducks was almost two-fold higher than that in breeder chickens (68.57% vs. 34.30%). Notably, co-infection by H9N2 avian influenza virus, infectious bursal disease virus, and/or chicken infectious anemia virus was very common in the 443 FAdV-4-positive cases. Furthermore, phylogenetic analysis of the hexon genes of four Shandong FAdV-4 isolates revealed that these strains clustered into Indian reference strains, indicating that the Shandong FAdV-4 strains might have originated in India. These findings provide the first data on the prevalence and co-infection status of FAdV-4 in Shandong province, which may serve as a foundation for the prevention of FAdV-4 in the field.

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SEROLOGICAL STUDIES ON CHICKEN INFECTIOUS ANEMIA VIRUS IN GHARBEIA PROVINCE

Kafrelsheikh Veterinary Medical Journal ◽

10.21608/kvmj.2008.116233 ◽

2008 ◽

Vol 6 (2) ◽

pp. 196-208

Author(s):

Moshira, A. El-abasy ◽

Gamal, R. Hassab El-nabby ◽

Ahmed, S. Hegasy ◽

Salwa, M. Helmy

Keyword(s):

Chicken Infectious Anemia Virus ◽

Serological Studies ◽

Chicken Infectious Anemia ◽

Anemia Virus

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Genetic Heterogeneity among Chicken Infectious Anemia Viruses Detected in Italian Fowl

Animals ◽

10.3390/ani11040944 ◽

2021 ◽

Vol 11 (4) ◽

pp. 944

Author(s):

Giulia Quaglia ◽

Giulia Mescolini ◽

Elena Catelli ◽

Giacomo Berto ◽

Filippo Muccioli ◽

...

Keyword(s):

Dna Amplification ◽

Molecular Characteristics ◽

Distribution Map ◽

Present Survey ◽

Broiler Breeder ◽

Non Invasive ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus ◽

Backyard Chicken

Chicken infectious anemia virus (CIAV) is a pathogen of chickens associated with immunosuppression and with a disease named chicken infectious anemia. The present survey reports an epidemiological study on CIAV distribution in Italian broiler, broiler breeder and backyard chicken flocks. Twenty-five strains were detected by a specifically developed nested PCR protocol, and molecularly characterized by partial VP1 gene or complete genome sequencing. Viral DNA amplification was successfully obtained from non-invasive samples such as feathers and environmental dust. Sequence and phylogenetic analysis showed the circulation of field or potentially vaccine-derived strains with heterogeneous sequences clustered into genogroups II, IIIa, and IIIb. Marker genome positions, reported to be correlated with CIAV virulence, were evaluated in field strains. In conclusion, this is the first survey focused on the molecular characteristics of Italian CIAVs, which have proved to be highly heterogeneous, implementing at the same time a distribution map of field viruses worldwide.

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Depletion of CD4 + and CD8 + T Lymphocyte Subpopulations by CIA-1, a Chicken Infectious Anemia Virus

Avian Diseases ◽

10.2307/1591677 ◽

1993 ◽

Vol 37 (2) ◽

pp. 492 ◽

Cited By ~ 26

Author(s):

Liang-biao Hu ◽

Benjamin Lucio ◽

Karel A. Schat

Keyword(s):

T Lymphocyte ◽

Lymphocyte Subpopulations ◽

T Lymphocyte Subpopulations ◽

Cd8 T Lymphocyte ◽

Chicken Infectious Anemia Virus ◽

Chicken Infectious Anemia ◽

Anemia Virus

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Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding δEF1

Journal of General Virology ◽

10.1099/vir.0.2008/003103-0 ◽

2008 ◽

Vol 89 (12) ◽

pp. 2998-3003 ◽

Cited By ~ 12

Author(s):

Myrna M. Miller ◽

Keith W. Jarosinski ◽

Karel A. Schat

Keyword(s):

Fluorescent Protein ◽

Transcription Start ◽

Upstream Promoter ◽

Chicken Infectious Anemia Virus ◽

E Box ◽

Promoter Construct ◽

Chicken Infectious Anemia ◽

Lmh Cells ◽

Green Fluorescent ◽

Anemia Virus

Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (δEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and δEF1.

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