scholarly journals The Use of Autologous Skeletal Muscle Derived Cells as a Sling in the Treatment of Induced Stress Urinary Incontinence, An Experimental Study in Dogs

2021 ◽  
Author(s):  
Bassem S Wadie ◽  
Haytham G Aamer ◽  
Sherry M Khater ◽  
Mahmoud M Gabr
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscles ◽  
Culture Medium ◽  
Canine Model ◽  
Polyglycolic Acid ◽  
Urethral Pressure ◽  
Histological Differentiation ◽  
Skeletal Muscle Biopsy ◽  
Induced Stress

Abstract Introduction:This is an experimental pre-clinical study, testing the applicability of autologous skeletal muscle derived cells as a treatment of SUI in canine modelMethods:10 Mongrel dogs included. Skeletal muscle biopsy was harvested in 4. 1 month later, incontinence was induced in 8 dogs through urethrolysis. Muscle biopsy was incubated and expanded for 8 weeks. Muscle derived cells were collected and covered a Polyglycolic acid (PGA) scaffold immersed in culture medium and coated with matrigel to be used as a sling. Placed suburethral in 8 dogs; 4 had cell- seeded and 4 had scaffold only. Urethral pressure (UP) measurement was done at baseline 2 &6 weeks after sling insertion. The urethra was harvested 4 weeks after sling insertion for histopathology.Results:UP shows increase of maximum urethral pressure during static measurement in all dogs with a scaffold inserted. The increase ranged from 5-40 cmH20 Histopathology shows significant periurethral proliferation of skeletal muscles in 4 dogs with cell-seeded scaffold. This was maximum in dogs # 1& 2. This was not the case in the 4 dogs that had sling only.Conclusion: Use of skeletal muscle –seeded PGA scaffold is a practical technique with preserved integrity of histological differentiation in canine model.

scholarly journals The use of autologous skeletal muscle-derived cells as a sling in the treatment of induced stress urinary incontinence, an experimental study

2021 ◽  
Author(s):  
Bassem S Wadie ◽  
Haytham Aamer ◽  
Sherry Khater ◽  
Mahmoud Gabr
Keyword(s):  
Skeletal Muscle ◽  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Muscle Biopsy ◽  
Culture Medium ◽  
Biceps Femoris ◽  
Canine Model ◽  
Polyglycolic Acid ◽  
Urethral Pressure ◽  
Skeletal Muscle Biopsy

Introduction & hypothesis: This is an experimental pre-clinical study testing for the applicability of autologous skeletal muscle derived cells as a seeded sling for the treatment of Stress urinary incontinence in canine model. Methods: 10 Mongrel dogs: In 4, skeletal muscle biopsy was harvested from Biceps Femoris. 1 month later, incontinence was induced in 8 dogs through surgical disruption of the pubourethral ligaments. Muscle biopsy was incubated in medium and after expansion for 8 weeks, Muscle derived cells were collected. Polyglycolic acid scaffold was immersed in culture medium, coated with matrigel and cells were seeded. The sling was placed suburethral in 8 dogs; 2 of which were cell-seeded and 4 had the scaffold only. Urethral pressure measurement was done at baseline and 2 weeks after insertion of the sling. The urethra with its surrounding was harvested 4 weeks after sling insertion for histopathology. 2 dogs were considered as control, in which no urethrolysis or insertion of slings was carried out. Results: Urethral pressure shows increase of maximum urethral pressure during static measurement in all dogs with a scaffold inserted. The increase ranged from 5-40 centimeter water (Median: 23 cmH20). Histopathology shows significant periurethral proliferation of skeletal muscles in 4 dogs with cell-seeded scaffold, as demonstrated by Desmin. This was maximum in dogs numbers 1and 2. This was not the case in the 4 dogs that had Polyglycolic acid sling only. Conclusion: The use of skeletal muscle-seeded scaffold is a practical technique with preserved integrity of histological differentiation in canine model at short term.

Internalized capillaries in skeletal muscle biopsy

2015 ◽  
Vol 25 (1) ◽  
pp. 94-95
Author(s):  
Andreas Hawlik ◽  
Anette Wassner ◽  
Albert C. Ludolph ◽  
Jan Lewerenz ◽  
Angela Rosenbohm
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Skeletal muscle biopsy

1982 ◽  
Vol 1 (3) ◽  
pp. 127-127 ◽  
Author(s):  
Victor J. Ojeda ◽  
Dominic V. Spagnolo ◽  
Keith Cole ◽  
Phillip F. Jacobsen
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

Enzymic Analysis of Human Skeletal Muscle Biopsy Samples

1981 ◽  
Vol 60 (3) ◽  
pp. 19P-19P
Author(s):  
F. Martin ◽  
J. Levi ◽  
G. Slavin ◽  
T. J. Peters
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Human Skeletal Muscle ◽  
Skeletal Muscle Biopsy

Role of repeat skeletal muscle biopsy: How useful is it?

2013 ◽  
Vol 47 (6) ◽  
pp. 835-839 ◽  
Author(s):  
Stephen A. Goutman ◽  
Richard A. Prayson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Skeletal Muscle Biopsy

scholarly journals Optimization of the pre-analytical stage of material processing for histochemical examination of skeletal muscle biopsies in the diagnosis of neuromuscular diseases

2019 ◽  
Vol 9 (2) ◽  
pp. 21-29
Author(s):  
A. M. Sycheva ◽  
V. D. Nazarova ◽  
S. V. Lapin ◽  
M. G. Rybakova ◽  
D. I. Rudenko
Keyword(s):  
Skeletal Muscle ◽  
Muscle Biopsy ◽  
Morphological Changes ◽  
Clinical Manifestations ◽  
Neuromuscular Diseases ◽  
Optimal Method ◽  
Tissue Samples ◽  
Important Place ◽  
Skeletal Muscle Biopsy ◽  
Muscle Biopsies

Diagnosis of neuromuscular diseases is complicated by the variety of clinical manifestations and requires the use of additional methods, an important place among which is the pathomorphological study of skeletal muscle biopsy. Despite the fact that the procedure for taking a muscle biopsy is not technically difficult, to obtain informative material a multitude of conditions must be observed at the stages of pre-analytical processing of the obtained tissue samples. Violation of the technology of taking, storing and fixing the material contributes to the formation of artifacts that limit the possibilities for further analysis of the morphological changes in tissue biopsy. A comparison was made of the effectiveness of various methods for cryoprocessing of muscle tissue samples and the manufacture of histological specimens with a subsequent assessment of morphological changes. As a result, the main causes of artifacts were identified. The optimal method for processing muscle biopsy specimens is indicated, which makes it possible to prevent the appearance of artifacts as much as possible and to ensure the preservation of tissue for research.

scholarly journals Gene expression profiling in human skeletal muscle during recovery from eccentric exercise

2008 ◽  
Vol 294 (6) ◽  
pp. R1901-R1910 ◽  
Author(s):  
D. J. Mahoney ◽  
A. Safdar ◽  
G. Parise ◽  
S. Melov ◽  
Minghua Fu ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Real Time ◽  
Muscle Damage ◽  
Muscle Biopsy ◽  
Muscle Growth ◽  
Lipid Synthesis ◽  
Rt Pcr ◽  
Protein Z ◽  
Skeletal Muscle Biopsy ◽  
Exercise Induced

We used cDNA microarrays to screen for differentially expressed genes during recovery from exercise-induced muscle damage in humans. Male subjects ( n = 4) performed 300 maximal eccentric contractions, and skeletal muscle biopsy samples were analyzed at 3 h and 48 h after exercise. In total, 113 genes increased 3 h postexercise, and 34 decreased. At 48 h postexercise, 59 genes increased and 29 decreased. On the basis of these data, we chose 19 gene changes and conducted secondary analyses using real-time RT-PCR from muscle biopsy samples taken from 11 additional subjects who performed an identical bout of exercise. Real-time RT-PCR analyses confirmed that exercise-induced muscle damage led to a rapid (3 h) increase in sterol response element binding protein 2 ( SREBP-2), followed by a delayed (48 h) increase in the SREBP-2 gene targets Acyl CoA:cholesterol acyltransferase ( ACAT)-2 and insulin-induced gene 1 ( insig-1). The expression of the IL-1 receptor, a known regulator of SREBP-2, was also elevated after exercise. Taken together, these expression changes suggest a transcriptional program for increasing cholesterol and lipid synthesis and/or modification. Additionally, damaging exercise induced the expression of protein kinase H11, capping protein Z alpha ( capZα), and modulatory calcineurin-interacting protein 1 ( MCIP1), as well as cardiac ankryin repeat protein 1 ( CARP1), DNAJB2, c-myc, and junD, each of which are likely involved in skeletal muscle growth, remodeling, and stress management. In summary, using DNA microarrays and RT-PCR, we have identified novel genes that respond to skeletal muscle damage, which, given the known biological functions, are likely involved in recovery from and/or adaptation to damaging exercise.

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