A Unique Breast Cancer Cell Model for Studying Reported Functions of Membrane-Localized Estrogen Receptor

2006 ◽  
Author(s):  
Adrian Lee
Keyword(s):  
Breast Cancer ◽  
Estrogen Receptor ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Model ◽  
Breast Cancer Cell Model

Potential Mechanisms of miR-143/Krupple Like Factor 5 Axis in Impeding the Proliferation of Michigan Cancer Foundation-7 Breast Cancer Cell Line

2021 ◽  
Vol 11 (2) ◽  
pp. 326-332
Author(s):  
Le Ma ◽  
Zhenyu Liu ◽  
Zhimin Fan
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Breast Cancer Cells ◽  
Cell Model ◽  
Cancer Cell Line ◽  
Breast Cancer Cell Model ◽  
Underlying Mechanisms ◽  
Mcf 7

Breast cancer is one of the most prevailing cancers in females, while the cancerous heterogeneity hinders its early diagnosis and subsequent therapy. miR-143-3p is a critical mediator in malignancy development and tumorigenesis as a tumor suppressor. Its role in various tumor entities has been investigated, such as colon cancer and breast cancer. Using MCF-7 breast cancer cell model, we planned to explore the underlying mechanisms of miR-143/KLF-5 axis in retarding breast cancer cells growth. Bioinformatics analysis searched the target KLF5 of miR-143, and the miR-143-targeted mimic and inhibitor were employed to detect the changes of KLF5. After transfection of mimic miR-143, the CCK-8 reagent assessed cell proliferation. Based on optimal stimulation time, miR-143 stimulation model was established, followed by determining expression of KLF5, EGFR and PCNA via western blot and qPCR. Eventually, siRNA-KLF5 was applied to silencing KLF5 level to evaluate its role in MCF-7 cells. The transcription and translation levels of KLF5 were diminished in miR-143-mimic transfected MCF-7 cells, while enhanced in miR-143-inhibitor transfected MCF-7 cells. When MCF-7 cells were transfected with miR-143-mimic at different time points, 48 hours was found to be the optimal transfection time, with reduced transcription and translation levels of KLF5, EGFR and PCNA. The transcription and translation levels of PNCA and EGFR were declined after silencing KLF5 by siRNA. miR-143/KLF5 axis could retard the proliferation of MCF-7 breast cancer cells.

The effect of tomatine on metastasis related matrix metalloproteinase (MMP) activities in breast cancer cell model

Gene ◽  
2017 ◽  
Vol 627 ◽  
pp. 408-411 ◽  
Author(s):  
Besra Özmen Yelken ◽  
Tuğçe Balcı ◽  
Sunde Yılmaz Süslüer ◽  
Çağla Kayabaşı ◽  
Çığır Biray Avcı ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Matrix Metalloproteinase ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Model ◽  
Breast Cancer Cell Model

Effects on AMPK Activation in a Breast Cancer Cell Model by 1,25D3

2011 ◽  
pp. P1-205-P1-205
Author(s):  
Luz E Tavera-Mendoza ◽  
Thomas Westerling ◽  
Myles Brown
Keyword(s):  
Breast Cancer ◽  
Cancer Cell ◽  
Breast Cancer Cell ◽  
Cell Model ◽  
Ampk Activation ◽  
Breast Cancer Cell Model
Sign in / Sign up

Export Citation Format

Share Document