Molecular Profiling of Prostate Cancer Specimens Using Multicolor Quantum Dots

2009 ◽  
Author(s):  
Xiaohu Gao
Keyword(s):  
Prostate Cancer ◽  
Quantum Dots ◽  
Molecular Profiling

Multicolor Quantum Dots in Molecular Profiling of Cancer Cells and Tissues

2009 ◽  
Author(s):  
Pavel Zrazhevskiy ◽  
Xiaohu Gao
Keyword(s):  
Quantum Dots ◽  
Cancer Cells ◽  
Molecular Profiling

A novel aptamer functionalized CuInS2 quantum dots probe for daunorubicin sensing and near infrared imaging of prostate cancer cells

2014 ◽  
Vol 818 ◽  
pp. 54-60 ◽  
Author(s):  
Zihan Lin ◽  
Qiang Ma ◽  
Xiaofang Fei ◽  
Hao Zhang ◽  
Xingguang Su
Keyword(s):  
Prostate Cancer ◽  
Quantum Dots ◽  
Cancer Cells ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Prostate Cancer Cells ◽  
Near Infrared Imaging ◽  
Cuins2 Quantum Dots

Automated quantum dots ISH assay for detection of ERG rearrangement and PTEN deletion in prostate cancer.

2013 ◽  
Vol 31 (15_suppl) ◽  
pp. 5045-5045
Author(s):  
Lei Tang ◽  
Wenjun Zhang ◽  
Antony Hubbard ◽  
Patrick S Brunhoeber ◽  
Yixin Wang
Keyword(s):  
Prostate Cancer ◽  
Quantum Dots ◽  
Emission Spectra ◽  
Pten Gene ◽  
Unfavorable Clinical Outcome ◽  
Pten Deletion ◽  
Prostate Cancers ◽  
Photo Stability ◽  
In Situ Detection ◽  
Erg Rearrangement

5045 Background: ERG rearrangement and PTEN deletion are the two most common genomic events in prostate cancer. Overexpression of the ERG protein caused by rearrangement of the ERG gene has been frequently associated with more aggressive prostate cancers and a poor prognosis. PTEN genomic deletion and absence of PTEN expression are associated with unfavorable clinical outcome. To detect ERG rearrangement and PTEN deletion simultaneously, we developed a four-color multiplex ISH assay using non-organic quantum dots (QD). The photo-stability and narrow emission spectra of QDs makes them desirable for ultrasensitive and multiplexing ISH applications. The automated QD ISH assay allows adequate delivery of QDs to nuclear targets and reproducible detection of ERG and PTEN gene targets. Methods: DNA probes specific for ERG 5’, ERG 3’, PTEN and CEN 10 were labeled with different haptens, digoxigenin (DIG), dinitrophenyl (DNP), thiazole sulfonamide (TS), or nitropyrazole (NP). QDs with 565, 655, 605 and 525nm wavelengths were conjugated to the anti- DIG, DNP, TS or NP antibodies, respectively. The multiplex QD ISH assay was fully automated on the VENTANA BenchMark ULTRA. FFPE prostate tissue slides were hybridized with the labeled probes. The probes were then detected by the QD-conjugated antibodies and visualized under fluorescent microscope. Results: We performed the multiplex QD ISH assay on 386 slides with tissue sections from 10 prostate specimens on 13 BenchMark ULTRA instruments. The 10 cases consisted of either benign prostate tissues or prostate cancer, positive or negative for ERG rearrangement and/or PTEN deletion. 350 (91%) of the slides were successfully stained for all 4 molecular targets. The expected ERG and PTEN status were detected with high reproducibility. Conclusions: We developed an automated QD based multiplex ISH assay to simultaneously detect ERG rearrangement and PTEN deletion in prostate cancer. The assay is highly sensitive and reproducible. It enables investigation of potential clinical use of ERG and PTEN as predictive or prognostic markers. In addition, the same technology is expected to enable multiplex in situ detection of other molecular biomarkers using standard clinical specimens.

Molecular profiling of single cells and tissue specimens with quantum dots

2003 ◽  
Vol 21 (9) ◽  
pp. 371-373 ◽  
Author(s):  
Xiaohu Gao ◽  
Shuming Nie
Keyword(s):  
Quantum Dots ◽  
Single Cells ◽  
Molecular Profiling ◽  
Tissue Specimens

ROS-mediated apoptotic cell death in prostate cancer LNCaP cells induced by biosurfactant stabilized CdS quantum dots

Biomaterials ◽  
2012 ◽  
Vol 33 (23) ◽  
pp. 5753-5767 ◽  
Author(s):  
Braj R. Singh ◽  
Brahma N. Singh ◽  
W. Khan ◽  
H.B. Singh ◽  
A.H. Naqvi
Keyword(s):  
Prostate Cancer ◽  
Quantum Dots ◽  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Cds Quantum Dots ◽  
Lncap Cells
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