scholarly journals Plant Regeneration from Protoplasts Isolated from Embryogenic Callus of Satsuma

HortScience ◽  
1990 ◽  
Vol 25 (8) ◽  
pp. 970-972 ◽  
Author(s):  
Jing-Tian Ling ◽  
Nobumasa Nito ◽  
Masao Iwamasa ◽  
Hisato Kunitake
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Embryogenic Callus ◽  
Colony Formation ◽  
Somatic Embryos ◽  
Malt Extract ◽  
Citrus Unshiu

Embryos were obtained from unfertilized and undeveloped seeds of satsuma (Citrus unshiu Marc.) cultured on a modified Murashige and Tucker (MT) medium. Embryogenic callus was induced from the hypocotyl region of the embryos. The callus was successfully maintained through subculturing on MT medium with 185 μm ade-nine, 2.8 μm GA3 and 400 mg malt extract/liter, solidified with Gelrite. Somatic embryogenesis occurred from callus subculture on medium containing 50 g lactose/liter and in the absence of plant growth regulators. Somatic embryos developed into plants on medium with sucrose and GA3. Protoplasts isolated from this callus produced somatic embryos through colony formation: subsequently, normal, entire plants were regenerated.

Somatic Embryogenesis and Plant Development in Eight Cultivars of Pecan

HortScience ◽  
1990 ◽  
Vol 25 (5) ◽  
pp. 573-576 ◽  
Author(s):  
I.E. Yates ◽  
C.C. Reilly
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Plant Development ◽  
Fruit Development ◽  
Somatic Embryos ◽  
Carya Illinoensis ◽  
Appreciable Variation

The influence of stage of fruit development and plant growth regulators on somatic embryogenesis and the relation of cultivar response on somatic embryogenesis and subsequent plant development have been investigated in eight cultivars of pecan [Carya illinoensis (Wangenh.) C. Koch]. Explants from the micropylar region of the ovule were more embryogenic when removed from fruits in the liquid endosperm stage than were intact ovules from less-mature fruits or from cotyledonary segments of more-mature fruits. Explants conditioned on medium containing auxin alone or auxin + cytokinin produced more somatic embryos than medium containing cytokinin alone. Under the conditions of this study, frequency of embryogenesis, as well as the germination of somatic embryos leading to plant development, indicated appreciable variation among cultivars. Plant development was greatest by far from somatic embryos of `Schley' than other cultivars studied.

scholarly journals EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS

2017 ◽  
Vol 17 (1) ◽  
pp. 9
Author(s):  
Yosi Zendra Joni ◽  
Riry Prihatini ◽  
Darda Efendi ◽  
Ika Roostika
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Malt Extract ◽  
Mass Propagation ◽  
Embryogenic Callus Induction

<p>Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.</p>

scholarly journals EFFECT OF PLANT GROWTH REGULATORS ON SOMATIC EMBRYOGENESIS OF EGGPLANT (SOLANUM MELONGENA L.)

2009 ◽  
Vol 12 (17) ◽  
pp. 71-80
Author(s):  
Nam Ngoc Trinh ◽  
Sanh Du Nguyen
Keyword(s):  
Somatic Embryogenesis ◽  
Survival Rate ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Somatic Embryos ◽  
Solanum Melongena ◽  
Ms Medium

On the MS medium containing only 2,4-D, callus induction was inhibited. Moreover, these calli were friable and turned brown after two weeks of culture. the three-week old calli were then transferred to the MS medium containing NAA 0.5 mg/l and kinetin 1 mg/l. The somatic embryos with globular shape appeared after 10 days of culture, while the heart shape, torpedo_shape and cotyledonary_shape embryos appeared successively after 15 days of culture. The abnormal embryos occupied at a rate of 34.3% and rarely germinated to plantlets. On the MS medium without plant growth regulators or only with NAA, somatic embryos could not be induced. On MS medium supplemented with ethephon 3 mg/l somatic embryogenesis from calli was inhibited. Plantlets derived from the eggplant somatic embryos had a survival rate up to 95% when transferred to the pots.

scholarly journals In vitro organogenesis and plant regeneration of Passiflora xishuangbannaensis, a species with extremely small populations

2021 ◽  
Author(s):  
Yuan-yuan Meng ◽  
Shi-jie Song ◽  
Sven Landrein
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Basal Medium ◽  
Ex Situ ◽  
Naphthaleneacetic Acid ◽  
South American ◽  
South American Species

Abstract Passiflora xishuangbannaensis (Passifloraceae) is endemic to a few sites of Mengyang nature reserve in Yunnan, Xishuangbanna and less than 40 individuals have been recorded. Nine Passiflora species are endemic to Yunnan with most species occurring in South America, making P. xishuangbannaensis highly significant and emblematic to the conservation work in the region. This study is designed to provide the first protocol for in vitro organogenesis and plant regeneration for ex situ conservation and reintroduction for an Asian Passiflora species. Using internodes, petioles and tendrils we optimize calli formation and root elongation using several plant growth regulators, individually or in combination. We also assess the genetic stability of regenerated cells. The maximum callus induction and shoot bud differentiation were both achieved on half Murashige and Skoog basal medium supplemented with 4.44 µM 6-Benzylaminopurine and 1.08 µM 1-Naphthaleneacetic acid. The best rooting was achieved from 30 days old, regenerated shoots on half Murashige and Skoog basal medium supplemented with 1.08 µM 1-Naphthaleneacetic acid. Micropropagated plants were subjected to inter simple sequence repeat markers analyses. Collectively, 86 bands were generated from 6 primers of which 12 bands were polymorphic, showing genetic variation between the regenerated plantlets and the original plant. Response to plant growth regulators was more specific than most other studies using South American species, which could be explained by the morphological and physiological differences between South American and Asian Passiflora species

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration in Anther Culture of Rice

2004 ◽  
Vol 7 (3) ◽  
pp. 331-334 ◽  
Author(s):  
Md. Monirul Islam ◽  
Sanjay Kumar Adhikar . ◽  
Purnendu Gain . ◽  
Md. Mizanur Rahman . ◽  
Noor-e-Alam Siddique .
Keyword(s):  
Plant Regeneration ◽  
Plant Growth ◽  
Anther Culture ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction
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