753 PB 143 POLLEN-PISTIL INCOMPATIBILITY DIFFERENCES BETWEEN MANZANILLO AND FRANTOIO OLIVE (Olea europaea L.) CULTIVARS

We have compared reproductive processes and fruit set in Manzanillo and Frantoio olive cultivars which are reported in the literature respectively as incompatible and partially compatible. The same incompatibility reaction was observed in both cultivars. Pollen tube growth was almost completely inhibited beyond the stigma, but some degree of self-fertilization was accomplished. However, in both cultivars cross-pollination provided a earlier and higher level of fertilization. Differences in self-incompatibility behavior seemed related to the level and the amount of delay in self-fertilization. In the compatible variety, Frantoio, self-pollen tube growth was accomplished more rapidly and showed a higher level of self-fertilization than in the incompatible Manzanillo cultivar. Fruit set matched reproductive behavior.

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Compatibility Relationships in `Manzanillo' Olive

HortScience ◽

10.21273/hortsci.32.6.1056 ◽

1997 ◽

Vol 32 (6) ◽

pp. 1056-1058 ◽

Cited By ~ 15

Author(s):

Julián Cuevas ◽

Vito S. Polito

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Olea Europaea ◽

Fruit Set ◽

Fold Increase ◽

Tube Growth ◽

Self Incompatibility ◽

Central California ◽

Self Pollination ◽

Olea Europaéa

We investigated pollination and fruit set parameters in `Manzanillo' olive (Olea europaea L.) following self-pollination and pollination with `Sevillano', `Ascolano', and `Mission' pollen. Results of analyses and experiments conducted over 2 years in central California indicated that `Manzanillo' behaves as a self-incompatible cultivar (index of self-incompatibility = 0.22 to 0.24). Pollination with `Sevillano' resulted in a more than 4-fold increase in fruit set over self-pollination. When `Mission' or `Ascolano' pollen was used, there was no increase over self-pollinated samples. Analyses of pollen tube growth, fertilization, initial fruit set, and final fruit set were consistent with `Manzanillo' being considered as a self-incompatible cultivar cross-incompatible with `Mission' and `Ascolano'. Our results indicate that `Manzanillo' is likely to be more productive when interplanted with `Sevillano' rather than when planted without a pollinizer or with `Mission' or `Ascolano'.

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Comparison of different classical and molecular methods for identifying self-incompatibility in two olive cultivars

Spanish Journal of Agricultural Research ◽

10.5424/sjar/2019173-14761 ◽

2019 ◽

Vol 17 (3) ◽

pp. e0804 ◽

Cited By ~ 1

Author(s):

Elham Aslmoshtaghi ◽

Ali Reza Shahsavar ◽

Majid Talebi ◽

Aziz Dazeh

Keyword(s):

Pollen Tube ◽

Molecular Analysis ◽

Pollen Tube Growth ◽

Tube Growth ◽

Research Station ◽

Self Incompatibility ◽

Controlled Pollination ◽

Cross Pollination ◽

Self Pollination ◽

Olive Cultivars

Aim of study: To determine compatibility relationships and select suitable pollinizers for two olive (Olea europaea L.) cultivars (‘Amygdalolia’ and ‘Konservalia’).Area of study: The Kazeroun Olive Research Station, Fars, Iran.Material and methods: Emasculated flowers from ‘Amygdalolia’ and ‘Konservalia’ cultivars were treated with self-pollination, open-pollination, and cross-pollination with pollen from cultivars such as ‘Dacal’, ‘Amygdalolia’,‘Konservalia’, ‘Koroniki’, and ‘Manzanilla’. Controlled pollination, pollen tube growth, and molecular analysis were employed.Main results: Controlled pollination, pollen tube growth, and molecular analysis showed that cross-pollination was beneficial for ‘Amygdalolia’ compared to self-pollination. The results showed that this cultivar is self-incompatible, and its best pollinator is the ‘Dacal’ cultivar. Experiment results indicated that ‘Konservalia’ behaves as a self-compatible cultivar. The highest fruit percentage and higher pollen tube growth rates were found in self-pollination treatments. Molecular attempts to isolate candidates for sporophytic self-incompatibility (SSI) led the researchers to analyze the expression of SRK and SLG genes.Research highlights: The results indicated an antagonist transcriptional expression pattern in the flowers of ‘Amygdalolia’, classified as a self-incompatible cultivar, and ‘Konservalia’, classified as a self-compatible cultivar, for the SRK and SLG genes.

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Polyamines Involved in Regulating Self-Incompatibility in Apple

Genes ◽

10.3390/genes12111797 ◽

2021 ◽

Vol 12 (11) ◽

pp. 1797

Author(s):

Jie Yu ◽

Baoan Wang ◽

Wenqi Fan ◽

Songbo Fan ◽

Ya Xu ◽

...

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Pollen Tubes ◽

Inhibitory Effect ◽

Tube Growth ◽

Calcium Signal ◽

Self Incompatibility ◽

Sequencing Data ◽

Incompatibility Reaction ◽

Polyamine Oxidases

Apple exhibits typical gametophytic self-incompatibility, in which self-S-RNase can arrest pollen tube growth, leading to failure of fertilization. To date, there have been few studies on how to resist the toxicity of self-S-RNase. In this study, pollen tube polyamines were found to respond to self-S-RNase and help pollen tubes defend against self-S-RNase. In particular, the contents of putrescine, spermidine, and spermine in the pollen tube treated with self-S-RNase were substantially lower than those treated with non-self-S-RNase. Further analysis of gene expression of key enzymes in the synthesis and degradation pathways of polyamines found that the expression of DIAMINE OXIDASE 4 (MdDAO4) as well as several polyamine oxidases such as POLYAMINE OXIDASES 3 (MdPAO3), POLYAMINE OXIDASES 4 (MdPAO4), and POLYAMINE OXIDASES 6 (MdPAO6) were significantly up-regulated under self-S-RNase treatment, resulting in the reduction of polyamines. Silencing MdPAO6 in pollen tubes alleviates the inhibitory effect of self-S-RNase on pollen tube growth. In addition, exogenous polyamines also enhance pollen tube resistance to self-S-RNase. Transcriptome sequencing data found that polyamines may communicate with S-RNase through the calcium signal pathway, thereby regulating the growth of the pollen tubes. To summarize, our results suggested that polyamines responded to the self-incompatibility reaction and could enhance pollen tube tolerance to S-RNase, thus providing a potential way to break self-incompatibility in apple.

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The influence of RNA-synthesis inhibitors on in vivo pollen tube growth and the self-incompatibility reaction in Lilium longiflorum thunb

Theoretical and Applied Genetics ◽

10.1007/bf00277048 ◽

1971 ◽

Vol 41 (2) ◽

pp. 75-78 ◽

Cited By ~ 12

Author(s):

Peter D. Ascher

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Rna Synthesis ◽

Lilium Longiflorum ◽

The Self ◽

Tube Growth ◽

Self Incompatibility ◽

Incompatibility Reaction

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The effects of pollinizers on pollen tube growth and fruit set of European plum (Prunus domestica L.) in a Nordic climate

Scientia Horticulturae ◽

10.1016/j.scienta.2021.110390 ◽

2021 ◽

Vol 288 ◽

pp. 110390

Author(s):

Radosav Cerović ◽

Milica Fotirić Akšić ◽

Milena Đorđević ◽

Mekjell Meland

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Fruit Set ◽

Tube Growth ◽

Prunus Domestica

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Testing of self-(in)compatibility in apricot cultivars from European breeding programmes

Horticultural Science ◽

10.17221/219/2012-hortsci ◽

2013 ◽

Vol 40 (No. 2) ◽

pp. 65-71 ◽

Cited By ~ 3

Author(s):

D. Milatović ◽

D. Nikolić ◽

B. Krška

Keyword(s):

Fluorescence Microscopy ◽

Pollen Tube ◽

Pollen Tube Growth ◽

Reliable Method ◽

Pollen Tubes ◽

Tube Growth ◽

Self Incompatibility ◽

Breeding Programmes

Self-(in)compatibility was tested in 40 new apricot cultivars from European breeding programmes. Pollen-tube growth in pistils from laboratory pollinations was analysed using the fluorescence microscopy. Cultivars were considered self-compatible if at least one pollen tube reached the ovary in the majority of pistils. Cultivars were considered self- incompatible if the growth of pollen tubes in the style stopped along with formation of characteristic swellings. Of the examined cultivars, 18 were self-compatible and 22 were self-incompatible. Fluorescence microscopy provides a relatively rapid and reliable method to determine self-incompatibility in apricot cultivars.      

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Pollen tube incompatibility reaction on the stigma in selfpollinated Sinapis alba L.

Acta Societatis Botanicorum Poloniae ◽

10.5586/asbp.1996.017 ◽

2014 ◽

Vol 65 (1-2) ◽

pp. 101-105 ◽

Cited By ~ 1

Author(s):

Renata Śnieżko ◽

Krystyna Winiarczyk

Keyword(s):

Pollen Tube ◽

Pollen Tube Growth ◽

Pollen Grains ◽

Pollen Tubes ◽

Sinapis Alba ◽

Pollen Grain ◽

Tube Growth ◽

Sinapis Alba L ◽

Growth Changes ◽

Incompatibility Reaction

After selfpollination of <em>Sinapis alba</em> L. pollen tubes growth is inhibited on the stigma. The pollen grains germinate 3-4 hours after pollination. The pollen give rise to one or more pollen tubes. They grow along the papillae. In the place of contact between the papilla and pollen tube the pellicula is digested. Then the direction of pollen tube growth changes completely. Pollen tubes grow back on the exine of their own pollen grain, or turn into the air. The pollen tubes growth was inhibited in 6-8 hours after selfpollination. After crosspollination usually there is no incompatibility reaction.

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