The influence of RNA-synthesis inhibitors on in vivo pollen tube growth and the self-incompatibility reaction in Lilium longiflorum thunb

1971 ◽  
Vol 41 (2) ◽  
pp. 75-78 ◽  
Author(s):  
Peter D. Ascher
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Rna Synthesis ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Incompatibility Reaction

Pollen‒pistil interaction in Lilium longiflorum : the role of the pistil in controlling pollen tube growth following cross- and self-pollinations

1982 ◽  
Vol 215 (1198) ◽  
pp. 45-62 ◽  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Active Component ◽  
Lilium Longiflorum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
System A ◽  
Pollen Tube Elongation

By cytophysiological methods, the self-incompatibility mechanism of the breeding system in Lilium longiflorum has been examined with particular reference to the synthesis, location and nature of the stylar factors involved in the control of pollen tube development. A ‘bioassay’ has been developed by which the effect of stylar extracts on pollen tube elongation may be investigated. With use of this system, a crude fraction of proteins from the stylar fluid has been shown to inhibit pollen tube growth only when protein fractions from ‘self’ styles are used. The proteins of this fraction have been analysed by thin-layer gel electrofocusing. Changes in the profiles thus obtained following selfing and a heat treatment known to inactivate the self-incompatibility response indicate a highly polarized glycoprotein to be an active component of the system. The various ways by which such a glycoprotein could control pollen tube elongation are considered in detail, and these events in Lilium are discussed in the light of our knowledge of other self-incompatibility systems operating in angiosperms.

scholarly journals Treatment of isolated pistils with protease inhibitors overcomes the self-incompatibility response in buckwheat

2007 ◽  
Vol 59 (1) ◽  
pp. 45-49 ◽  
Author(s):  
Jovanka Miljus-Djukic ◽  
Svetlana Radovic ◽  
Vesna Maksimovic
Keyword(s):  
Pollen Tube ◽  
Protease Inhibitors ◽  
Pollen Tube Growth ◽  
Fluorescence Microscope ◽  
Fagopyrum Esculentum ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Fagopyrum Esculentum Moench ◽  
Pepstatin A

Isolated pistils of distylous buckwheat (Fagopyrum esculentum Moench) were treated with protease inhibitors (PMSF, pepstatin A, and antipain). Pistils were cross- or self- pollinated, and growth of pollen tubes was observed under a fluorescence microscope. Treatments with all inhibitors suppressed inhibition of self-pollen tube growth, suggesting that activity of proteases is involved in rejection of self-pollen during the SI response.

scholarly journals Pollen tube growth and fruit set in quince (Cydonia oblonga Mill.)

2020 ◽  
Vol 18 (2) ◽  
pp. e0702
Author(s):  
Aleksandar Radovic ◽  
Radosav Cerovic ◽  
Dragan Milatovic ◽  
Dragan Nikolic
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Pollen Tubes ◽  
The Self ◽  
Tube Growth ◽  
Open Pollination ◽  
Self Pollination ◽  
High Yields

Aim of study: To determine the self-compatibility level of eight quince cultivars.Area of study: The region of Belgrade (Central Serbia).Material and methods: Pollen tube growth in vivo and fruit set in two pollination variants (self- and open-pollination) were studied in eight quince cultivars. The quantitative parameters of pollen tube growth (average number of pollen tubes in the upper and middle third of the style, base of the style and in the ovary; the dynamics of pollen tube growth through these parts of the pistil) was determined using the fluorescence microscopy.Main results: The parameters of pollen tube growth and fruit set were primarily dependent on the genotype and variants of pollination. All studied parameters were significantly higher in the open-pollination variant compared with the self-pollination in all cultivars. In the self-pollination variant, ʻLeskovackaʼ and ʻVranjskaʼ had the highest number of pollen tubes that penetrated the ovary (2.10 and 0.54 in average, respectively), as well as the largest percentage of pistils with the penetration of pollen tubes in the nucellus of ovules six days after pollination (40.09% and 14.74%). Also, they had the highest percentage of initial fruit set (17.01% and 28.52%) and final fruit set (9.32% and 9.86%). Based on this, ʻLeskovackaʼ and ʻVranjskaʼ can be classified as self-compatible cultivars, while the others are self-incompatible.Research highlights: The majority of quince cultivars were self-incompatible. When establishing new orchards with these cultivars, care should be taken about the choice of pollenisers in order to achieve high yields.

scholarly journals Seasonal Changes in the Self-Incompatibility and Pollen Tube Growth in Japanese Pears (Pyrus serotina Rehd.)

1985 ◽  
Vol 53 (4) ◽  
pp. 377-382 ◽  
Author(s):  
Shin HIRATSUKA ◽  
Makoto HIROTA ◽  
Eikichi TAKAHASHI ◽  
Naomi HIRATA
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Seasonal Changes ◽  
The Self ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Pyrus Serotina

scholarly journals Polyamines Involved in Regulating Self-Incompatibility in Apple

Genes ◽  
2021 ◽  
Vol 12 (11) ◽  
pp. 1797
Author(s):  
Jie Yu ◽  
Baoan Wang ◽  
Wenqi Fan ◽  
Songbo Fan ◽  
Ya Xu ◽  
...  
Keyword(s):  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Pollen Tubes ◽  
Inhibitory Effect ◽  
Tube Growth ◽  
Calcium Signal ◽  
Self Incompatibility ◽  
Sequencing Data ◽  
Incompatibility Reaction ◽  
Polyamine Oxidases

Apple exhibits typical gametophytic self-incompatibility, in which self-S-RNase can arrest pollen tube growth, leading to failure of fertilization. To date, there have been few studies on how to resist the toxicity of self-S-RNase. In this study, pollen tube polyamines were found to respond to self-S-RNase and help pollen tubes defend against self-S-RNase. In particular, the contents of putrescine, spermidine, and spermine in the pollen tube treated with self-S-RNase were substantially lower than those treated with non-self-S-RNase. Further analysis of gene expression of key enzymes in the synthesis and degradation pathways of polyamines found that the expression of DIAMINE OXIDASE 4 (MdDAO4) as well as several polyamine oxidases such as POLYAMINE OXIDASES 3 (MdPAO3), POLYAMINE OXIDASES 4 (MdPAO4), and POLYAMINE OXIDASES 6 (MdPAO6) were significantly up-regulated under self-S-RNase treatment, resulting in the reduction of polyamines. Silencing MdPAO6 in pollen tubes alleviates the inhibitory effect of self-S-RNase on pollen tube growth. In addition, exogenous polyamines also enhance pollen tube resistance to self-S-RNase. Transcriptome sequencing data found that polyamines may communicate with S-RNase through the calcium signal pathway, thereby regulating the growth of the pollen tubes. To summarize, our results suggested that polyamines responded to the self-incompatibility reaction and could enhance pollen tube tolerance to S-RNase, thus providing a potential way to break self-incompatibility in apple.

X-RAY INACTIVATION OF THE STYLAR COMPONENT OF THE SELF-INCOMPATIBILITY REACTION IN LILIUM LONGIFLORUM

1968 ◽  
Vol 10 (4) ◽  
pp. 941-944 ◽  
Author(s):  
J. E. Hopper ◽  
S. J. Peloquin
Keyword(s):  
Growth Rate ◽  
Pollen Tube ◽  
Pollen Tube Growth ◽  
Lilium Longiflorum ◽  
Tube Growth ◽  
X Ray ◽  
Pollen Tube Growth Rate ◽  
X Irradiation ◽  
Incompatibility Reaction ◽  
Incompatible Pollen Tube

Styles of self-incompatible Lilium longiflorum cultivars were X-irradiated to determine the effect on subsequent pollen tube growth. Incompatible pollen tube growth rate in styles exposed to 24 to 70 kr prior to pollination was as high as that of compatible tubes growing in untreated styles. No effect was detected following 6 kr or less; following 6 kr to 24 kr there was a corresponding increase in growth rate. The growth rate of compatible tubes was unaffected by X-irradiation of the styles. Inactivation of the stylar component of the incompatibility reaction by X-irradiation provides a method for obtaining self-seed as well as a possible means of exploring the biochemical nature of this pollen-style interaction.

scholarly journals 753 PB 143 POLLEN-PISTIL INCOMPATIBILITY DIFFERENCES BETWEEN MANZANILLO AND FRANTOIO OLIVE (Olea europaea L.) CULTIVARS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 541a-541 ◽  
Author(s):  
J. Cuevas ◽  
L. Rallo ◽  
H.F. Rapoport
Keyword(s):  
Pollen Tube ◽  
Reproductive Behavior ◽  
Pollen Tube Growth ◽  
Fruit Set ◽  
Tube Growth ◽  
Self Incompatibility ◽  
Olive Cultivars ◽  
Self Fertilization ◽  
Incompatibility Reaction ◽  
Reproductive Processes

We have compared reproductive processes and fruit set in Manzanillo and Frantoio olive cultivars which are reported in the literature respectively as incompatible and partially compatible. The same incompatibility reaction was observed in both cultivars. Pollen tube growth was almost completely inhibited beyond the stigma, but some degree of self-fertilization was accomplished. However, in both cultivars cross-pollination provided a earlier and higher level of fertilization. Differences in self-incompatibility behavior seemed related to the level and the amount of delay in self-fertilization. In the compatible variety, Frantoio, self-pollen tube growth was accomplished more rapidly and showed a higher level of self-fertilization than in the incompatible Manzanillo cultivar. Fruit set matched reproductive behavior.

Sign in / Sign up

Export Citation Format

Share Document