scholarly journals ALLOZYME DIVERSITY IN CHESTNUT (CASTANEA SPP.).

HortScience ◽  
1994 ◽  
Vol 29 (7) ◽  
pp. 736a-736
Author(s):  
Hongwen Huang ◽  
Fenny Dane ◽  
Joseph Norton

Allozyme genetic variability in three chestnut (Castanea) species was investigated using 19 loci from ten enzyme systems. G-tests of heterogeneity of isozymic allele distribution showed significant differences in both intraspecific and interspecific populations. C. mollissima was found to possess a significantly higher value of mean gene heterozygosity (H=0.3050±0.0419), percentage of polymorphic loci (P=84.21%) and average number of alleles per locus (A=2.05) than any other species in the Castanea section Eucustanon. When the genetic variability of populations of C. mollissimo from four regions in China was investigated, population from the Changjiang river region showed a markedly higher mean gene heterozygosity (H=0.3480±0.0436) than populations from the other regions. An approximately identical genetic distance between the population from the Changjiang river region and populations from three other regions was observed, while populations from the latter regions showed almost the same genetic distance from each other.

2009 ◽  
Vol 7 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Juliana S. Philippsen ◽  
Erasmo Renesto ◽  
Ana Maria Gealh ◽  
Roberto F. Artoni ◽  
Oscar A. Shibatta ◽  
...  

Four samples of Neoplecostomus yapo were analyzed through the allozyme electrophoresis technique in corn starch gel. The allozyme pattern was similar to those found in N. paranensis with 24 loci scored. Two samples (ribeirão Atlântico and ribeirão Uraí) showed monomorphic bands for all 24 loci, whereas the other two (rio Verde and rio Fortaleza) showed 8.3% of polymorphic loci. The He genetic variability estimates for the rios Verde and Fortaleza populations were 0.0195 and 0.0179, respectively, too much inferior to the mean heterozygosity summed to species from the whole world (0.051). The Wright statistical values F IS = 0.5181, F IT = 0.5681 and F ST = 0.1039 and the genetic distance of Nei values showed that the four samples are genetically very similar to each other and that there is homozygote excess in the polymorphic loci.


1994 ◽  
Vol 84 (2) ◽  
pp. 269-273
Author(s):  
Tomáš Pavlíček

AbstractGenetic variability in four polymorphic loci and variation in phenotypic plasticity in relation to the relative size of wings, were compared and contrasted among eight local populations of Sitonasulcifrons Thunberg from the Czech and Slovak republics. The distances between localities from which samples were taken were between 49 and 492 kilometres. The results demonstrated that: 1. The variability between populations was 5.5% and the other 94.5% of the total variability was realized within populations. 2. Significant differences in allozyme frequencies among populations existed when distances were more than 100 km. A similar trend was also found in variability of relative wing size in males among populations when distances were more than 200 km. 3. Overall, a significant deficiency of heterozygotes in relation to Hardy-Weinberg predictions existed from all samples. The results support the idea that, at least inagricultural areas, gene flow in S. sulcifrons does not seem to be strong enough to stabilize one large interbreeding population in distances over 100 kilometres.


1993 ◽  
Vol 41 (3) ◽  
pp. 355 ◽  
Author(s):  
J Playford ◽  
JC Bell ◽  
GF Moran

A study of allozyme variation in Acacia melanoxylon R. Br. in 27 populations from across the geographic range of the species indicated high genetic diversity compared to other Australian trees and plants generally. Clines of increasing genetic variability with increasing latitude were found for four measures of genetic diversity. Most of the genetic diversity is found within populations but there was an unusually high level of the variation between populations (37.7%). A distinct genetic separation between the northern and southern populations was located approximately at the Hunter River region, where there is also a disjunction in the distribution of the species. The Nei (1978) genetic distance between these populations within this species is larger than observed between some plant species. Clearly the species has evolved separately in the two regions for a considerable time.


2017 ◽  
Vol 16 (4) ◽  
pp. 531-537 ◽  
Author(s):  
Stefano Pallotti ◽  
Antonietta La Terza ◽  
Attilio De Cosmo ◽  
Dario Pediconi ◽  
Irene Pazzaglia ◽  
...  

2016 ◽  
Vol 14 (1) ◽  
pp. 122-133
Author(s):  
Md. Harun-Or-Rashid ◽  
Md. Shafikur Rahman ◽  
Sudhir Chandra Nath ◽  
S S R M Mahe Alam Sorwar ◽  
Md. Tanvir Ahmed

Seven individuals of introgressed Brassica lines (Binasarisha-5/Daulot) and two of their parental lines were used for this study to estimate genetic variability using three randomly amplified polymorphic DNA (RAPD) markers (61AB10G1, 72AB10G12 and 73AB10T13). A total of 23 clear bands were scored, of which 21 (91.30%) bands were proved to be polymorphic. The highest proportion of polymorphic loci and gene diversity values were 43.48% and 0.187, respectively in the line five of Binasarisha-5/Daulot. The lower proportion of polymorphic loci and gene diversity values were 8.70% and 0.034; 8.70% and 0.026 in the line seven of the cross and one parent, Daulot, respectively. The co-efficient of gene differentiation (Gst) and gene flow (Nm) values were 0.677 and 0.237, found respectively from the Popgene analysis. Result of cluster analysis indicated that the nine accessions were capable of being classified into two major groups - one consists of only one parent Daulot (Brassica juncea) while another consists of Binasarisha-5 (Brassica napus) and all introgressed lines of C6 generation (treated with colchicine in C1 generation) resulted from the cross B. napus and B. juncea. Introgressed line seven and Binasarisha-5 showed the lowest genetic distance of 0.077. Higher similarity was found between Binasarisha-5 and introgressed progenies. Introgressed line one and Daulot showed the highest genetic distance of 0.709, which can be used as germplasm for breeding program that aim to improve Brassica. It was concluded that RAPD markers can be used for the study of molecular characterization and diversity in Brassica.The Agriculturists 2016; 14(1) 122-133


1995 ◽  
Vol 16 (4) ◽  
pp. 331-340 ◽  
Author(s):  
Ross D. MacCulloch ◽  
F.D. Danielyan ◽  
Ilya S. Darevsky ◽  
Robert W. Murphy ◽  

AbstractGenetic diversity at 37 allozyme loci was surveyed from Lacerta valentini (4 populations), L. portschinskii and L. rudis (1 population each). The number of polymorphic loci ranged from 1 (L. valentini) to 11 (L. rudis). Mean heterozygosity (direct count) ranged from 0.003 (L. valentini) to 0.071 (L. rudis). Nei's (1978) genetic distance ranged from 0-0.03 among populations of L. valentini, 0.127-0.163 between L. valentini and L. rudis and 0.366-0.487 between L. portschinskii and the two other taxa. Indices of genetic variability for species having disjunct distributions were lower than in species with contiguous distributions, similar to the case of insular populations, which have lower values than do mainland populations.


2000 ◽  
Vol 80 (1) ◽  
pp. 9-17 ◽  
Author(s):  
A. Farid ◽  
E. O'Reilly ◽  
C. Dollard ◽  
C.R. Kelsey Jr.

The genetic variability of 257 sheep from 10 breeds; North Country Cheviot (NCC), Cheviot (CHE), Dorset (DOR), Suffolk (SUF), Scottish Blackface (SBF), Texel (TEX), Romanov (ROM), Finnish Landrace (FIN), Icelandic (ICE) and Red Masai (MAS) was assessed using 10 microsatellite loci. The average number of alleles per locus was 4.3 in ROM, 5.0 in MAS, and spanned a narrow range (5.4 to 6.0) in the other breeds. Estimates of expected heterozygosity (HE) of the breeds varied within 0.05 point of each other (0.62 in FIN to 0.67 in CHE), except for ROM (0.53) which was lower (P < 0.05) than those of the other breeds, and in MAS (0.57), which was lower (P < 0.05) than those of NCC, CHE and SBF. Estimates of observed heterozygosity (HO) of the breeds were the lowest in MAS, ROM and SUF (0.50 to 0.53) and the highest in NCC and CHE (0.64 and 0.67). The HE was greater (P < 0.01) than HO only in SUF. The results suggest that there have not been drastic losses of genetic variability in the intensely selected breeds. The low genetic variability of ROM was probably due to a small number of animals imported to North America. The British breeds (NCC, CHE, SUF, DOR, SBF) were genetically close to each other, as were the North European breeds (ROM, ICE, FIN). MAS was remotely related to the British breeds, but it was surprisingly close to the North European breeds. TEX was more closely related to the British breeds than to the North European breeds. More than 90% of 1000 simulated individuals from each breed were assigned to the correct breed, indicating that this panel of markers is useful for the identification of breed membership of individual animals, and could be used to protect the integrity of registered breeds. Key words: Sheep, genetic variability, genetic distance, microsatellites


2010 ◽  
Vol 43 (4) ◽  
pp. 409-415 ◽  
Author(s):  
Raquel Borges Moroni ◽  
Juracy de Freitas Maia ◽  
Wanderli Pedro Tadei ◽  
Joselita Maria Mendes dos Santos

INTRODUCTION: Isoenzymatic analyses were performed involving species of the Nyssorhynchus and Anopheles subgenera in order to estimate the intra and interspecies genetic variability. METHODS: Mosquitoes were caught at different localities in the Amazon region. The collection and rearing of mosquitoes in the laboratory followed specific protocols. For the genetic variability analyses, the technique of horizontal electrophoresis on starch and starch-agarose gel with appropriate buffer systems was used. The alloenzyme variation was estimated using the Biosys-1 software. RESULTS: Out of the 13 loci, eight were polymorphic. Anopheles nuneztovari presented the largest number of alleles per locus, while the smallest number was detected in Anopheles marajoara from Macapá. The largest number of polymorphic loci was found for Anopheles marajoara from Maruanum and the smallest for Anopheles benarrochi (Guayará Mirim). Anopheles darlingi (Macapá) presented the greatest heterozygosity (Ho = 0.167 ± 0.071), while the lowest heterozygosity (Ho = 0.045 ± 0.019) was observed in Anopheles intermedius (Pacoval) of the subgenus Anopheles. Wright's F coefficient revealed considerable genetic structuring between the populations of Anopheles darlingi (Fst = 0.110) and between the populations of Anopheles marajoara (Fst = 0.082). CONCLUSIONS: Considering all the species studied, the genetic distance ranged from 0.008 to 1.114. The greatest distance was between Anopheles mattogrossensis and Anopheles oswaldoi, while the smallest was between the Anopheles benarrochi populations.


2013 ◽  
Vol 20 (1-2) ◽  
pp. 1-8
Author(s):  
MM Rahman ◽  
L Rahman ◽  
SN Begum ◽  
F Nur

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F3 rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F3 line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F3 lines. So, relatively high level of genetic variation was found in 05-6 F3 line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (Nm) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Nei’s genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F3 lines and they remain in different cluster.DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839 Progress. Agric. 20(1 & 2): 1 – 8, 2009


2010 ◽  
Vol 30 (3) ◽  
pp. 1-6 ◽  
Author(s):  
Andong WANG ◽  
Shaoming PAN ◽  
Yongzhan ZHANG ◽  
Zhiyong LIU

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